##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939992_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1465508 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.81790478114074 34.0 31.0 34.0 31.0 34.0 2 32.916514955906074 34.0 31.0 34.0 31.0 34.0 3 32.94114395827249 34.0 31.0 34.0 31.0 34.0 4 36.35546377092449 37.0 37.0 37.0 35.0 37.0 5 36.24720574708565 37.0 37.0 37.0 35.0 37.0 6 36.37176255605565 37.0 37.0 37.0 35.0 37.0 7 36.368774513683995 37.0 37.0 37.0 35.0 37.0 8 36.39429808639734 37.0 37.0 37.0 35.0 37.0 9 38.23788133534583 39.0 39.0 39.0 37.0 39.0 10-11 38.2264433220426 39.0 39.0 39.0 37.0 39.0 12-13 38.205935075072944 39.0 39.0 39.0 37.0 39.0 14-15 39.756965161568544 41.0 40.0 41.0 37.0 41.0 16-17 39.7729214033632 41.0 40.0 41.0 37.0 41.0 18-19 39.806664992616895 41.0 40.0 41.0 37.5 41.0 20-21 39.81411974550804 41.0 40.0 41.0 37.5 41.0 22-23 39.76522066068558 41.0 40.0 41.0 37.0 41.0 24-25 39.73201920426227 41.0 40.0 41.0 37.0 41.0 26-27 39.64562731830874 41.0 40.0 41.0 37.0 41.0 28-29 39.56751720222613 41.0 40.0 41.0 37.0 41.0 30-31 39.45752735570191 41.0 39.5 41.0 36.5 41.0 32-33 39.32676928409808 41.0 39.0 41.0 36.0 41.0 34-35 39.24468341353305 41.0 39.0 41.0 35.0 41.0 36-37 39.23976361780352 41.0 39.0 41.0 35.5 41.0 38-39 39.31005835519151 41.0 40.0 41.0 35.0 41.0 40-41 39.260471454267055 41.0 40.0 41.0 35.0 41.0 42-43 39.16606460012501 41.0 40.0 41.0 35.0 41.0 44-45 39.03008581324701 41.0 39.0 41.0 35.0 41.0 46-47 38.86537910403764 41.0 39.0 41.0 35.0 41.0 48-49 38.68489902477503 41.0 39.0 41.0 35.0 41.0 50-51 38.55098470973888 41.0 39.0 41.0 35.0 41.0 52-53 38.438618554112296 41.0 39.0 41.0 35.0 41.0 54-55 38.330173905567214 41.0 39.0 41.0 35.0 41.0 56-57 38.19825446193402 41.0 38.0 41.0 34.0 41.0 58-59 38.04339894425687 40.0 38.0 41.0 34.0 41.0 60-61 37.86983319094812 40.0 37.5 41.0 34.0 41.0 62-63 37.65846416396226 40.0 37.0 41.0 34.0 41.0 64-65 37.42717405841524 40.0 36.5 41.0 34.0 41.0 66-67 37.16397488106513 39.0 36.0 41.0 33.0 41.0 68-69 36.84261498401919 39.0 35.5 41.0 33.0 41.0 70-71 36.49233576343493 38.5 35.0 40.5 33.0 41.0 72-73 36.13373349036648 37.0 35.0 40.0 32.0 41.0 74-75 35.756728383604866 37.0 35.0 39.0 32.0 41.0 76-77 35.357904903965036 36.5 35.0 39.0 32.0 41.0 78-79 34.97169616269581 36.0 35.0 38.5 31.5 39.5 80-81 34.66282374439444 35.5 35.0 37.0 31.5 39.0 82-83 34.40194014635198 35.0 35.0 37.0 32.0 39.0 84-85 34.11650055816823 35.0 35.0 36.5 31.0 38.0 86-87 33.88576827966821 35.0 35.0 36.0 31.0 37.0 88-89 33.670950619170966 35.0 35.0 36.0 31.0 37.0 90-91 33.50423539141376 35.0 35.0 35.5 31.0 36.5 92-93 33.36483014763481 35.0 35.0 35.0 31.0 36.0 94-95 33.26187540429667 35.0 34.5 35.0 31.0 36.0 96-97 33.17628972342696 35.0 34.0 35.0 31.0 36.0 98-99 33.0925081951105 35.0 34.0 35.0 31.0 36.0 100-101 33.04859031816953 35.0 34.0 35.0 31.0 36.0 102-103 32.223486668104165 34.5 33.0 35.0 28.0 35.0 104-105 32.8970537861274 35.0 34.0 35.0 30.5 35.0 106-107 32.97159722089542 35.0 34.0 35.0 31.0 35.0 108-109 32.969866762924525 35.0 34.0 35.0 31.0 35.0 110-111 32.93857420089144 35.0 34.0 35.0 31.0 35.0 112-113 32.87550119139575 35.0 34.0 35.0 31.0 35.0 114-115 32.83351029131195 35.0 34.0 35.0 31.0 35.0 116-117 32.78553034169721 35.0 34.0 35.0 30.5 35.0 118-119 32.71795718617708 35.0 34.0 35.0 30.0 35.0 120-121 32.62910506118015 35.0 34.0 35.0 30.0 35.0 122-123 32.474645310704545 35.0 34.0 35.0 30.0 35.0 124-125 30.52902679480426 33.5 30.5 34.5 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.009483172878688606 1101 2 0.030455496685576122 1101 3 0.04723010510302572 1101 4 0.052416265368144366 1101 5 -0.11759086239933225 1101 6 -0.0033942973949478983 1101 7 -0.009797955138182601 1101 8 0.0615831783594345 1101 9 -0.03550663133436416 1101 10-11 0.050304144260685746 1101 12-13 0.027458466283690086 1101 14-15 0.16327753073618112 1101 16-17 0.11329162659328063 1101 18-19 0.1227834382870654 1101 20-21 0.09082569312901967 1101 22-23 0.08572346606363368 1101 24-25 0.12006074227809904 1101 26-27 0.1924131111126144 1101 28-29 0.23045606750455505 1101 30-31 0.13266609083713377 1101 32-33 0.17179298440376556 1101 34-35 0.1747946998819856 1101 36-37 0.1601547551353235 1101 38-39 0.026885111365942294 1101 40-41 0.09503856458744053 1101 42-43 0.0014760339491814989 1101 44-45 0.07719154806890316 1101 46-47 0.12133328374063979 1101 48-49 0.11806205170973527 1101 50-51 0.1788640038554945 1101 52-53 0.14593706393991113 1101 54-55 0.08122977616946514 1101 56-57 0.11891343167457791 1101 58-59 0.13132209859751498 1101 60-61 0.11284598249004318 1101 62-63 0.03776669187472237 1101 64-65 0.07124101053467058 1101 66-67 0.08010084025477937 1101 68-69 0.028085438542213126 1101 70-71 0.07047121882490615 1101 72-73 0.04798918614066139 1101 74-75 0.04773377307245852 1101 76-77 0.11254362858839784 1101 78-79 0.13235752833363534 1101 80-81 2.1592635633993496E-4 1101 82-83 0.03063070299781856 1101 84-85 6.146365456274339E-4 1101 86-87 -0.00819766454475257 1101 88-89 -0.012164230025057066 1101 90-91 0.09706015458587558 1101 92-93 0.13268564946380224 1101 94-95 0.1257785466703396 1101 96-97 0.02350391589080658 1101 98-99 0.09582369297555715 1101 100-101 0.06539519868030652 1101 102-103 0.1204668020666233 1101 104-105 0.10194498622918502 1101 106-107 0.07199062740131978 1101 108-109 0.07083048651924173 1101 110-111 0.04388376818818074 1101 112-113 0.09130199974848097 1101 114-115 0.017205607840836024 1101 116-117 0.04789922372332711 1101 118-119 0.16383226397949358 1101 120-121 0.1884751667591118 1101 122-123 0.08633749809680324 1101 124-125 0.22163945511674754 1102 1 0.0388240603905885 1102 2 0.06900373048699748 1102 3 0.08620219867751189 1102 4 0.011958571174602639 1102 5 0.02066271449845658 1102 6 0.05093225588613137 1102 7 0.007395321665022436 1102 8 0.0069719604829643345 1102 9 0.01134381634427939 1102 10-11 0.12496736861589142 1102 12-13 0.10775072968093724 1102 14-15 -1.3795641208957932E-4 1102 16-17 0.14488521385518283 1102 18-19 0.050861475890677355 1102 20-21 0.0484181045518568 1102 22-23 0.08144687432378106 1102 24-25 0.091954354404578 1102 26-27 0.0037284706846705262 1102 28-29 -0.025352019758507538 1102 30-31 -0.010590262992828059 1102 32-33 -0.11534958724457312 1102 34-35 -0.02837332659065339 1102 36-37 -0.02412966778562975 1102 38-39 -0.019262621425788495 1102 40-41 -0.03313625897860106 1102 42-43 -0.06525887476063019 1102 44-45 -0.1259058351082274 1102 46-47 -0.13766188509450217 1102 48-49 -0.2012656286107557 1102 50-51 -0.22921129167282572 1102 52-53 -0.17160594365191173 1102 54-55 -0.29163982885550155 1102 56-57 -0.2741293656140229 1102 58-59 -0.21081337386439003 1102 60-61 -0.2135901794411268 1102 62-63 -0.28386514501362825 1102 64-65 -0.265376267792945 1102 66-67 -0.19513659325570387 1102 68-69 -0.20465703178876282 1102 70-71 -0.19648526351078743 1102 72-73 -0.20629170461552349 1102 74-75 -0.16645681510268417 1102 76-77 -0.11302715297276933 1102 78-79 -0.1303185280043948 1102 80-81 -0.14121776073569237 1102 82-83 -0.11654414612994657 1102 84-85 -0.11382970978313267 1102 86-87 -0.13521291559568738 1102 88-89 -0.20602628040023063 1102 90-91 -0.19784051489877186 1102 92-93 -0.21078147235637346 1102 94-95 -0.18251727677719742 1102 96-97 -0.1578521797657899 1102 98-99 -0.22670384977641334 1102 100-101 -0.1382799955306524 1102 102-103 -0.14611671155428496 1102 104-105 -0.19833026700611356 1102 106-107 -0.10940218417860592 1102 108-109 -0.11980962539460904 1102 110-111 -0.10625821554062043 1102 112-113 -0.0862474912247464 1102 114-115 -0.15483405465152345 1102 116-117 -0.14473412130643482 1102 118-119 -0.09958473363396791 1102 120-121 -0.039211892736950915 1102 122-123 -0.027643716363698445 1102 124-125 0.03217192895665022 1103 1 0.0444994940244996 1103 2 0.05942404560308745 1103 3 0.08525648394025609 1103 4 0.042769769499848564 1103 5 -0.10367002866979647 1103 6 0.012360653897133034 1103 7 0.009967922277482444 1103 8 0.0018984494282463515 1103 9 0.1143802271633163 1103 10-11 0.028436668961056455 1103 12-13 0.044712051345335624 1103 14-15 0.04976973963074727 1103 16-17 0.06801479899126406 1103 18-19 0.10775839092293182 1103 20-21 0.08162302162717339 1103 22-23 0.03948831261641317 1103 24-25 0.13758308635893002 1103 26-27 0.1328070802160184 1103 28-29 0.13646655143120512 1103 30-31 0.034383637662465105 1103 32-33 0.07886629865582506 1103 34-35 0.047605656508324046 1103 36-37 0.059989012657148066 1103 38-39 -0.03755323459433413 1103 40-41 0.044519541168440924 1103 42-43 0.02885016301070209 1103 44-45 -0.10393703521999242 1103 46-47 0.04051830688855773 1103 48-49 -0.05000486649614544 1103 50-51 -0.10004427189963394 1103 52-53 0.016650840001815936 1103 54-55 0.0918163473786322 1103 56-57 -0.012794645812647332 1103 58-59 -0.06728186569102235 1103 60-61 -0.04524860441037504 1103 62-63 -0.03141004298682759 1103 64-65 -0.020922292653175134 1103 66-67 0.04013130581002855 1103 68-69 0.006282246866113894 1103 70-71 0.04373097511441415 1103 72-73 0.020102531769644827 1103 74-75 -0.027348453005487272 1103 76-77 -0.023627220311141173 1103 78-79 0.013937096696416518 1103 80-81 -0.07227426312574181 1103 82-83 -0.005825434995522016 1103 84-85 -0.009232033644039461 1103 86-87 0.006126888939803621 1103 88-89 -0.038255592747198364 1103 90-91 0.037828665886429746 1103 92-93 0.08776697347861528 1103 94-95 0.031742997477785195 1103 96-97 -0.008484335899190398 1103 98-99 -0.008478055515752203 1103 100-101 0.038079540979794047 1103 102-103 -0.028320027625483135 1103 104-105 -0.03380075378923664 1103 106-107 0.03988961771832322 1103 108-109 0.014156742066759875 1103 110-111 0.09328381482577441 1103 112-113 0.13266531474683774 1103 114-115 0.03841676245608738 1103 116-117 0.03988318373867372 1103 118-119 0.09890313057156419 1103 120-121 0.14537724013265318 1103 122-123 0.1477788090084431 1103 124-125 0.15690538123917364 1104 1 0.03173414184527701 1104 2 0.03521683929666608 1104 3 0.03638438831392676 1104 4 -0.022690183125867236 1104 5 -0.02226638189401342 1104 6 -0.04258453474613333 1104 7 -0.027118559239553974 1104 8 -0.038640895383807106 1104 9 -0.036247035259606264 1104 10-11 -0.021384416577227228 1104 12-13 -4.2989453967123836E-4 1104 14-15 -0.022795581402142773 1104 16-17 0.0021356538519938795 1104 18-19 -0.009890144801111944 1104 20-21 0.08584171314483768 1104 22-23 0.035793556438243 1104 24-25 0.02538173060848692 1104 26-27 -0.010017339395908209 1104 28-29 0.05279694237965771 1104 30-31 0.03431019601892871 1104 32-33 0.08448546922537048 1104 34-35 0.16280639552591936 1104 36-37 0.13711324583348272 1104 38-39 0.11381165180559805 1104 40-41 0.12005250186654592 1104 42-43 0.07802221908472973 1104 44-45 0.1425466142810876 1104 46-47 0.1681776907397179 1104 48-49 0.1826085986310133 1104 50-51 0.15252950377066554 1104 52-53 0.2709249445667865 1104 54-55 0.26221578143555746 1104 56-57 0.28354669944359756 1104 58-59 0.2506183625089804 1104 60-61 0.2966913743003943 1104 62-63 0.20387773765094863 1104 64-65 0.22738611623804417 1104 66-67 0.23040710130248243 1104 68-69 0.27489041269203796 1104 70-71 0.19766101000361402 1104 72-73 0.2323037547942164 1104 74-75 0.21769047548978193 1104 76-77 0.20583699192989968 1104 78-79 0.22555023354828307 1104 80-81 0.2135883753405139 1104 82-83 0.1727060020096829 1104 84-85 0.14733648077307748 1104 86-87 0.1311202526467099 1104 88-89 0.16550813663715047 1104 90-91 0.10869553830767842 1104 92-93 0.08962978094913154 1104 94-95 0.1526773831207464 1104 96-97 0.21105839696169681 1104 98-99 0.27553615411501653 1104 100-101 0.21120461121390122 1104 102-103 0.11579725248537187 1104 104-105 0.1947107079737691 1104 106-107 0.21387762726396176 1104 108-109 0.22916224134163343 1104 110-111 0.19415569101201413 1104 112-113 0.15966053838548788 1104 114-115 0.2033587302851103 1104 116-117 0.16360658215781854 1104 118-119 0.19618930699608228 1104 120-121 0.13597494008194388 1104 122-123 0.11705752800921232 1104 124-125 0.25165184829794285 1105 1 0.043329660826238126 1105 2 0.05801914733024205 1105 3 0.004434520689933663 1105 4 0.04617206184566669 1105 5 -0.055028913503505805 1105 6 0.03866553417761054 1105 7 -0.10104913839413143 1105 8 0.041975287148325435 1105 9 0.05694614039881429 1105 10-11 0.027943142147925926 1105 12-13 0.05438410041637809 1105 14-15 0.019876382284166993 1105 16-17 0.03558021243888021 1105 18-19 -0.0011492353130506672 1105 20-21 0.04039085004988152 1105 22-23 0.05183592948494464 1105 24-25 -0.04542034668959616 1105 26-27 0.026709580056198945 1105 28-29 0.0474012734206255 1105 30-31 0.0040528835275708275 1105 32-33 0.021496799865090566 1105 34-35 0.012889400923612016 1105 36-37 0.07548979682984225 1105 38-39 0.006487305563233292 1105 40-41 0.04610007918626735 1105 42-43 0.13398554401713625 1105 44-45 -0.012533299091309402 1105 46-47 -0.010511574715529548 1105 48-49 -0.06636528584345314 1105 50-51 -0.07426015961848975 1105 52-53 -0.017126583249989835 1105 54-55 -0.16270717241989274 1105 56-57 0.02848080410080911 1105 58-59 -0.14772091452545766 1105 60-61 -0.02439053340710018 1105 62-63 0.005393678334961294 1105 64-65 -0.0013228083903342736 1105 66-67 -0.0786809081712434 1105 68-69 -0.14042647960622645 1105 70-71 0.005330568781360512 1105 72-73 0.02319759186611492 1105 74-75 -0.050883519771652175 1105 76-77 -0.13910557444260974 1105 78-79 -0.0704975091884279 1105 80-81 -0.21237150976678265 1105 82-83 -0.09884496890322225 1105 84-85 -0.2528405154070583 1105 86-87 -0.2368823965700244 1105 88-89 -0.256092374543762 1105 90-91 -0.14568345237722724 1105 92-93 -0.21373034710907035 1105 94-95 -0.12055511512540562 1105 96-97 -0.1255524549018716 1105 98-99 -0.16486717916460236 1105 100-101 -0.11461840193717876 1105 102-103 -0.16808884222307086 1105 104-105 -0.154957906654694 1105 106-107 -0.13279874245234424 1105 108-109 -0.08339888871938683 1105 110-111 -0.1436853967022529 1105 112-113 -0.22667328243312568 1105 114-115 -0.08846653168875207 1105 116-117 -0.18798055145414594 1105 118-119 -0.29603365559677286 1105 120-121 -0.4297562581563241 1105 122-123 -0.3843354739189593 1105 124-125 -0.3597822618731712 1106 1 0.05086121716458791 1106 2 0.07465914666872919 1106 3 0.007295780297404519 1106 4 0.0243922967662229 1106 5 -0.09012869892453779 1106 6 -0.017135333234087113 1106 7 0.03278219178873343 1106 8 -0.007976959729113275 1106 9 0.04665999376572216 1106 10-11 0.03998633495064041 1106 12-13 0.09464887184223159 1106 14-15 -0.07127895386887673 1106 16-17 0.03574042313144332 1106 18-19 -0.016929161636802803 1106 20-21 0.04393191980251032 1106 22-23 0.0351345332746007 1106 24-25 0.05799276123245534 1106 26-27 0.06864829481501289 1106 28-29 0.05862573790346204 1106 30-31 0.08352586270224549 1106 32-33 0.06225936141700572 1106 34-35 0.1832877363861911 1106 36-37 0.1812261665175683 1106 38-39 0.0452336930705215 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-0.3032716928068453 2216 62-63 -0.27396324037487574 2216 64-65 -0.1837843888326418 2216 66-67 -0.24970052150384703 2216 68-69 -0.3776595626860271 2216 70-71 -0.34372898707029975 2216 72-73 -0.2567858761623256 2216 74-75 -0.26228083461091956 2216 76-77 -0.3241196877495156 2216 78-79 -0.4585038561897221 2216 80-81 -0.4041456578741105 2216 82-83 -0.23186992987678678 2216 84-85 -0.25910875506217224 2216 86-87 -0.28055107672332724 2216 88-89 -0.2006144091988844 2216 90-91 -0.24917616360284 2216 92-93 -0.19914119229503768 2216 94-95 -0.17918000238729093 2216 96-97 -0.21295369276203502 2216 98-99 -0.19768535760003658 2216 100-101 -0.18597724849107067 2216 102-103 -0.10504967048724723 2216 104-105 -0.2491320257026075 2216 106-107 -0.18403040091208567 2216 108-109 -0.1805705572081706 2216 110-111 -0.15168320307087413 2216 112-113 -0.07888294964967457 2216 114-115 -0.20926589592546918 2216 116-117 -0.3212882215447408 2216 118-119 -0.22200719026695737 2216 120-121 -0.23799539941519754 2216 122-123 -0.28433396779078635 2216 124-125 -0.3344654876807631 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 112.0 3 7.0 4 26.0 5 61.0 6 105.0 7 258.0 8 430.0 9 851.0 10 1617.0 11 2549.0 12 3144.0 13 4957.0 14 8148.0 15 8648.0 16 3610.0 17 2450.0 18 2218.0 19 2068.0 20 1965.0 21 2035.0 22 2186.0 23 2468.0 24 2960.0 25 3933.0 26 5084.0 27 6537.0 28 8535.0 29 11123.0 30 14258.0 31 18475.0 32 23901.0 33 31621.0 34 45146.0 35 72400.0 36 158742.0 37 542635.0 38 465692.0 39 4553.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.19832959455337 20.05858293983442 15.158335682156157 23.584751783456053 2 14.059834542015464 20.848879705876733 42.459065388929986 22.63222036317782 3 18.828351670547004 25.64052874498126 29.02154065347989 26.509578930991847 4 11.820133359899776 18.289084740581423 39.348062241898376 30.54271965762043 5 13.02812715633561 37.89500652451647 35.05605618599313 14.020810133154793 6 29.587965401758296 38.207979758554714 19.12715590771255 13.076898931974442 7 27.42734942422696 32.118623712733054 22.393122384865862 18.060904478174123 8 24.224364520698625 37.295395180374314 20.910018914942803 17.570221383984258 9 25.384235364119473 17.573496698755655 21.57709135671726 35.465176580407615 10-11 23.64330320953553 27.465390840582245 28.99062987032483 19.900676079557396 12-13 25.23333205959981 24.616174050226952 29.12846603362111 21.022027856552132 14-15 21.17204409665454 27.09548497858763 27.07770957238036 24.65476135237747 16-17 20.643326629171124 29.530350407623295 28.600467756050378 21.2258552071552 18-19 20.835607857480138 28.02386612696758 30.55858446354438 20.581941552007905 20-21 21.670615434587386 27.485198944205003 30.10656896650553 20.73761665470208 22-23 20.964664716029436 27.999275334628905 30.08442829224622 20.951631657095444 24-25 21.002955971581187 27.610187047767738 30.613753046725094 20.77310393392598 26-27 20.491188038550455 28.144711594887234 30.314709984524136 21.049390382038176 28-29 20.528547097661697 28.249862846193945 29.83095281636129 21.390637239783064 30-31 20.984259383094464 27.639153112777276 30.166297283945227 21.210290220183037 32-33 20.550621354506422 27.976510534231135 30.315358223905974 21.157509887356465 34-35 20.873069270177986 28.2523534501347 30.16786670560652 20.706710574080798 36-37 20.78105339581906 27.771803361018844 30.190111551762257 21.257031691399842 38-39 20.96573338391875 28.1691399842239 29.857905927500905 21.007220704356442 40-41 21.69165913799174 27.557816129287595 30.00420332062329 20.746321412097373 42-43 20.862458615033148 28.10148426347724 29.99031735070706 21.045739770782554 44-45 20.84447850165267 28.25286521806773 29.661796455563533 21.240859824716072 46-47 21.49360494790885 27.944064447276983 29.20594769868196 21.356382906132207 48-49 21.36597002541098 28.11202668289767 29.05739852665424 21.464604765037105 50-51 21.155633404935354 28.724237602251236 28.871592649101878 21.24853634371153 52-53 21.238915106570555 28.43027810151838 29.09093638519885 21.23987040671221 54-55 21.116670806300615 28.288518384068865 29.229113727117152 21.365697082513368 56-57 21.332363931141966 28.29786667831223 28.688516200525687 21.681253190020115 58-59 21.433045742500212 28.327344511254797 28.602539187776525 21.637070558468462 60-61 21.21622672820619 28.01537077927927 29.380289974534428 21.38811251798011 62-63 21.26651645709201 27.974224637463596 29.398952445158947 21.360306460285443 64-65 21.224449132996888 28.280500686451386 28.976709782546394 21.518340398005336 66-67 21.21288317771039 28.60305095570956 28.655524227776308 21.52854163880375 68-69 21.610731568848482 27.944678568796622 29.282474063601153 21.162115798753742 70-71 21.735398237334767 27.953719802280162 28.974150942881238 21.33673101750383 72-73 21.449456434219396 28.205919039677706 28.895611624092123 21.449012902010768 74-75 21.165356995662936 28.56149539954746 29.129489569487166 21.143658035302433 76-77 21.351674641148325 27.951092726890607 29.279164630967557 21.41806800099351 78-79 21.24379396086545 28.109706668267933 28.99530401744651 21.65119535342011 80-81 21.435468110716556 28.377531886554014 28.815844062263736 21.371155940465695 82-83 21.304523755585095 28.387699009490223 29.166541567838593 21.14123566708609 84-85 21.28111890211449 28.301619643154456 29.104992944426094 21.31226851030496 86-87 21.027725539539873 28.70772455694544 28.54283292892294 21.721716974591747 88-89 21.373885369441858 28.3056796687565 28.718710508574503 21.60172445322714 90-91 21.337636771193353 28.406715819569495 28.9568712278104 21.298776181426753 92-93 21.101044825412075 28.525262229888888 29.07609511514096 21.29759782955808 94-95 20.982560194555024 28.571584539862897 28.914300804231736 21.53155446135034 96-97 21.391763129235734 28.196024859639113 28.8502348673634 21.561977143761755 98-99 21.38841170600227 28.117367087743837 28.893184008734295 21.601037197519595 100-101 21.111738122028758 28.137531925811338 29.115589348942038 21.635140603217863 102-103 20.924310313827295 28.34070338283054 28.976901112825725 21.758085190516432 104-105 21.14203459478492 28.144615999798017 28.778810506584527 21.934538898832535 106-107 21.189549347781064 27.916696948077362 28.685691240159393 22.20806246398218 108-109 20.92313602585321 28.246996170943177 28.58619676705189 22.243671036151714 110-111 20.900446282345232 28.35714870619063 28.777295556283438 21.965109455180695 112-113 21.04090670402632 28.103607524385055 28.679151805775298 22.176333965813324 114-115 20.89414028646882 28.277638645312297 28.311383007035477 22.516838061183407 116-117 21.02670692192293 28.291691160891492 28.076198846590692 22.60540307059489 118-119 20.92656051902892 28.072249448313414 28.503397461354645 22.49779257130302 120-121 20.72844862292545 28.227094134553575 28.751148073897063 22.29330916862391 122-123 20.932070184010062 28.328672209065836 28.42184920338958 22.317408403534518 124-125 20.945335044705153 28.3446298885493 28.14295686565117 22.567078201094375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 62.0 1 49.0 2 77.0 3 121.5 4 86.0 5 61.5 6 144.5 7 347.5 8 614.5 9 720.0 10 570.0 11 426.5 12 445.0 13 518.5 14 602.0 15 725.0 16 873.0 17 1033.5 18 1207.5 19 1475.0 20 1827.0 21 2283.0 22 2880.5 23 3681.0 24 4822.5 25 6449.5 26 8496.0 27 10890.5 28 13623.0 29 16720.0 30 20403.5 31 25290.0 32 31244.5 33 37191.0 34 43322.5 35 49294.5 36 54637.5 37 58035.0 38 58289.5 39 57087.5 40 56523.0 41 56499.0 42 56138.5 43 55993.5 44 56231.5 45 56891.0 46 57149.5 47 57454.0 48 57700.5 49 55212.0 50 51363.0 51 47485.0 52 43076.0 53 39154.0 54 36073.5 55 32123.5 56 27293.5 57 22952.5 58 19345.5 59 15742.5 60 11987.0 61 9473.5 62 7676.0 63 5505.0 64 3599.5 65 2590.0 66 1981.5 67 1542.5 68 1152.5 69 817.5 70 604.5 71 422.5 72 299.5 73 272.5 74 230.5 75 142.0 76 77.0 77 43.5 78 27.0 79 16.0 80 7.5 81 5.0 82 7.0 83 4.5 84 1.5 85 2.0 86 1.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 1.0 97 1.5 98 6.0 99 8.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016035395234962894 2 0.0 3 0.0 4 0.0 5 0.12268783247856715 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.411786220204871E-5 18-19 0.0 20-21 0.004912972157095014 22-23 2.7294289761638967E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 8.870644172532665E-4 92-93 0.0 94-95 6.823572440409742E-4 96-97 0.0 98-99 0.0013988323502839971 100-101 6.823572440409742E-5 102-103 3.0706075981843837E-4 104-105 0.0018423645589106302 106-107 0.005970625885358525 108-109 2.3882503541434097E-4 110-111 0.004640029259478625 112-113 0.0076765189954609596 114-115 0.0053223865035195985 116-117 0.0018082466967085816 118-119 1.3647144880819483E-4 120-121 6.823572440409742E-4 122-123 0.0011941251770717048 124-125 0.0011600073148696562 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1465508.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.60472881532965 #Duplication Level Percentage of deduplicated Percentage of total 1 82.35181151607168 59.79130942575526 2 11.95839042944637 17.364713883955744 3 3.1470210395205944 6.854658274515887 4 1.1192028054023222 3.2503766470236712 5 0.48525074680674796 1.7615749439670063 6 0.2550648145150369 1.1111347012917967 7 0.1562496787296915 0.7941125886156144 8 0.09614314183165763 0.5584357392113023 9 0.07075305730786938 0.4623305884824001 >10 0.3258304417777521 4.167526084376512 >50 0.0197156878718897 0.9562430337254102 >100 0.013063053841865088 1.8695461561399256 >500 0.001221664337223583 0.5946218214720523 >1k 2.819225393592884E-4 0.4634161114673757 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3173 0.21651195353420113 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2221 0.15155154390150036 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 6.823572440409742E-5 0.0 7 0.0 0.0 0.0 6.823572440409742E-5 0.0 8 0.0 0.0 0.0 6.823572440409742E-5 0.0 9 0.0 0.0 0.0 6.823572440409742E-5 0.0 10-11 0.0 0.0 0.0 6.823572440409742E-5 0.0 12-13 0.0 0.0 0.0 6.823572440409742E-5 0.0 14-15 0.0 0.0 0.0 6.823572440409742E-5 0.0 16-17 0.0 0.0 0.0 6.823572440409742E-5 0.0 18-19 0.0 0.0 0.0 6.823572440409742E-5 0.0 20-21 0.0 0.0 0.0 1.3647144880819483E-4 0.0 22-23 0.0 0.0 0.0 1.3647144880819483E-4 0.0 24-25 0.0 0.0 0.0 3.0706075981843837E-4 0.0 26-27 0.0 0.0 0.0 4.4353220862663323E-4 0.0 28-29 0.0 0.0 0.0 9.211822794553152E-4 0.0 30-31 0.0 0.0 0.0 0.0020470717321229223 0.0 32-33 0.0 0.0 0.0 0.005902390160954427 0.0 34-35 0.0 0.0 0.0 0.009962415762998222 0.0 36-37 0.0 0.0 0.0 0.018594234900116545 0.0 38-39 0.0 0.0 0.0 0.032104908332127834 0.0 40-41 0.0 0.0 0.0 0.0489591322599399 0.0 42-43 0.0 0.0 0.0 0.06496040963270075 0.0 44-45 0.0 0.0 0.0 0.08389582315483778 0.0 46-47 0.0 0.0 0.0 0.10607243358616944 0.0 48-49 0.0 0.0 0.0 0.13254789465495923 0.0 50-51 0.0 0.0 0.0 0.1641751529162584 0.0 52-53 0.0 0.0 0.0 0.19464240386268788 0.0 54-55 0.0 0.0 0.0 0.2294085054465755 0.0 56-57 0.0 0.0 0.0 0.27028170436462984 0.0 58-59 0.0 0.0 0.0 0.31453257164068704 0.0 60-61 0.0 0.0 0.0 0.3632528788652126 0.0 62-63 0.0 0.0 0.0 0.41671556893582296 0.0 64-65 0.0 0.0 0.0 0.4750229954391242 0.0 66-67 0.0 0.0 0.0 0.5390281049301675 0.0 68-69 0.0 0.0 0.0 0.6121085657669558 0.0 70-71 0.0 0.0 0.0 0.6969596890634511 0.0 72-73 0.0 0.0 0.0 0.7848473020959286 0.0 74-75 0.0 0.0 0.0 0.8729055044394163 0.0 76-77 0.0 0.0 0.0 0.9707896510970939 0.0 78-79 0.0 0.0 0.0 1.0831738891906424 0.0 80-81 0.0 0.0 0.0 1.2062370181534323 0.0 82-83 0.0 0.0 0.0 1.3379660841155423 0.0 84-85 0.0 0.0 0.0 1.4720834004317958 0.0 86-87 0.0 0.0 0.0 1.6107383924209215 0.0 88-89 0.0 0.0 0.0 1.7618122862515935 0.0 90-91 0.0 0.0 0.0 1.9351992619624048 0.0 92-93 0.0 0.0 0.0 2.120186310821913 0.0 94-95 0.0 0.0 0.0 2.310222803287324 0.0 96-97 0.0 0.0 0.0 2.499986352855119 0.0 98-99 0.0 0.0 0.0 2.7168053671491386 0.0 100-101 0.0 0.0 0.0 2.9570974706381676 0.0 102-103 0.0 0.0 0.0 3.215608512543091 0.0 104-105 0.0 0.0 0.0 3.4766442762509655 0.0 106-107 0.0 0.0 0.0 3.73184588552229 0.0 108-109 0.0 0.0 0.0 4.002809947130961 0.0 110-111 0.0 0.0 0.0 4.308574228185721 0.0 112-113 0.0 0.0 0.0 4.644089285080668 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3250 0.0 42.52628 1 GTATCAA 5055 0.0 41.954792 1 ATCAACG 5775 0.0 36.512764 3 TCAACGC 5810 0.0 36.087765 4 CAACGCA 5915 0.0 35.447147 5 TATCAAC 6110 0.0 34.608326 2 AACGCAG 6200 0.0 33.873302 6 CGCAGAG 6985 0.0 29.896147 8 ACGCAGA 7010 0.0 29.874397 7 CGCGATC 40 0.0053661074 29.747091 22-23 GTACATG 5380 0.0 28.347218 1 TACATGG 5295 0.0 27.335924 2 ACATGGG 5050 0.0 26.656952 3 GCAGAGT 8105 0.0 25.6181 9 GAGTACT 4335 0.0 25.252432 12-13 GTCCTAC 2425 0.0 25.0577 1 GTAGGAC 2550 0.0 24.99407 3 GGACCGT 120 0.0012887657 24.789244 6 AGAGTAC 6890 0.0 23.70269 10-11 AGTACTT 4595 0.0 22.981974 12-13 >>END_MODULE