FastQCFastQC Report
Fri 27 May 2016
SRR939991_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939991_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179720
Sequences flagged as poor quality0
Sequence length125
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC4300.01958.2391108-109
CCGCGCC4800.01186.7028108-109
CTACGCG250.008321522990.63873108-109
CGTCGCT652.6318008E-5936.4704110-111
CCGCTCG1400.0884.49884108-109
CGTCGCC2250.0880.56775108-109
CCGCGCG1551.8189894E-12798.9022108-109
CTCCGCC2750.0774.7164110-111
CTATGCG654.890963E-5762.0298108-109
CCTCGCC6900.0753.7469108-109
TTGTTCG2450.0707.5991108-109
TTGTGCG1402.9905823E-7652.18475110-111
CGCCGCC4550.0602.01666110-111
AAGTTCG1254.3479668E-7594.38324108-109
CCGTGCG851.0930752E-4582.7287108-109
CGGCGCG901.297345E-4550.35486108-109
CCGCGCT951.525575E-4521.3888108-109
CGTGTCT1808.1636244E-7507.2548110-111
CAGCGCG2004.5456545E-9495.31937108-109
CCGCACG1001.779088E-4495.31937108-109
Produced by FastQC (version 0.11.5)