##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939991_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1179720 Sequences flagged as poor quality 0 Sequence length 125 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.534843013596447 33.0 31.0 34.0 30.0 34.0 2 31.61777540433323 34.0 31.0 34.0 29.0 34.0 3 31.757018614586514 34.0 31.0 34.0 30.0 34.0 4 35.0463982979012 37.0 35.0 37.0 33.0 37.0 5 35.05381955040179 37.0 35.0 37.0 32.0 37.0 6 35.19568117858475 37.0 35.0 37.0 33.0 37.0 7 35.100707795070015 37.0 35.0 37.0 33.0 37.0 8 35.09754687552979 37.0 35.0 37.0 33.0 37.0 9 36.74515732546706 39.0 38.0 39.0 33.0 39.0 10-11 36.727467534669245 39.0 38.0 39.0 33.0 39.0 12-13 36.66553292306649 39.0 38.0 39.0 33.0 39.0 14-15 38.123206777879496 41.0 39.0 41.0 33.0 41.0 16-17 38.10847150171227 41.0 39.0 41.0 33.0 41.0 18-19 38.10719196080426 41.0 39.0 41.0 33.0 41.0 20-21 38.16794154545146 41.0 39.0 41.0 33.0 41.0 22-23 38.12829739260164 41.0 39.0 41.0 33.0 41.0 24-25 38.139416556471026 41.0 39.0 41.0 33.0 41.0 26-27 38.112192299867765 41.0 39.0 41.0 33.0 41.0 28-29 38.055799681280305 41.0 39.0 41.0 33.0 41.0 30-31 37.97445580307192 40.0 38.0 41.0 33.0 41.0 32-33 37.8747647746923 40.0 38.0 41.0 33.0 41.0 34-35 37.78520369240159 40.0 38.0 41.0 33.0 41.0 36-37 37.94788848201268 40.5 38.0 41.0 33.0 41.0 38-39 37.924139626352016 41.0 38.0 41.0 33.0 41.0 40-41 37.869520733733424 41.0 38.0 41.0 33.0 41.0 42-43 37.75182755230055 40.5 38.0 41.0 32.5 41.0 44-45 37.621450852744715 40.0 38.0 41.0 32.0 41.0 46-47 37.52333901264707 40.0 38.0 41.0 32.0 41.0 48-49 37.41463355711525 40.0 38.0 41.0 32.0 41.0 50-51 37.2813057335639 40.0 37.5 41.0 31.5 41.0 52-53 37.147582053368595 40.0 37.0 41.0 31.0 41.0 54-55 36.98829976604618 40.0 37.0 41.0 31.0 41.0 56-57 36.80834138609161 40.0 36.5 41.0 31.0 41.0 58-59 36.62730393652731 40.0 36.0 41.0 30.5 41.0 60-61 36.39456057369545 39.5 35.5 41.0 30.0 41.0 62-63 36.132822618926525 39.0 35.0 41.0 30.0 41.0 64-65 35.83842479571423 39.0 35.0 41.0 30.0 41.0 66-67 35.53589283897874 38.5 35.0 40.5 29.0 41.0 68-69 35.184913369274064 37.5 35.0 40.0 29.0 41.0 70-71 34.81095047977486 37.0 35.0 40.0 29.0 41.0 72-73 34.422170515037465 36.5 34.0 39.0 28.5 41.0 74-75 34.00794552944767 36.0 34.0 39.0 28.0 41.0 76-77 33.58657562811514 35.5 34.0 38.5 27.5 40.0 78-79 33.165465110365176 35.0 34.0 37.0 26.5 39.0 80-81 32.76529134031804 35.0 34.0 37.0 26.0 39.0 82-83 32.596405926830094 35.0 34.0 36.5 27.0 39.0 84-85 32.215255738649844 35.0 34.0 36.0 26.0 37.0 86-87 31.57560904282372 35.0 34.0 36.0 13.0 37.0 88-89 16.218646373715796 18.0 16.5 18.5 2.0 19.0 90-91 2.715013732072017 2.0 2.0 2.0 2.0 2.0 92-93 2.2742125250059333 2.0 2.0 2.0 2.0 2.0 94-95 2.0953518631539687 2.0 2.0 2.0 2.0 2.0 96-97 2.000025429762995 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22762497942360582 1101 2 0.34142655703398717 1101 3 0.34958192858461246 1101 4 0.37971717661478976 1101 5 0.3466299666158932 1101 6 0.35114800591259865 1101 7 0.34500800602258863 1101 8 0.3502333194960485 1101 9 0.5154677911825871 1101 10-11 0.44424482793750997 1101 12-13 0.4881947895149992 1101 14-15 0.4570063803461011 1101 16-17 0.4882440947198461 1101 18-19 0.414477820067475 1101 20-21 0.4663228739159706 1101 22-23 0.4979568514572037 1101 24-25 0.3777159867159554 1101 26-27 0.49826009700895213 1101 28-29 0.5596791509533077 1101 30-31 0.4257802326714497 1101 32-33 0.5985366845771551 1101 34-35 0.5869247389323888 1101 36-37 0.5922903553899488 1101 38-39 0.5611066002721188 1101 40-41 0.5883839823286436 1101 42-43 0.49141590593433193 1101 44-45 0.4722833206136201 1101 46-47 0.45168284789055946 1101 48-49 0.5222677983960864 1101 50-51 0.5221641407284991 1101 52-53 0.5247942284280782 1101 54-55 0.5316983979397918 1101 56-57 0.5797943010698461 1101 58-59 0.4859862410876872 1101 60-61 0.49162973617096384 1101 62-63 0.4977473860799364 1101 64-65 0.4637668459295199 1101 66-67 0.5216466588498463 1101 68-69 0.5646219269168569 1101 70-71 0.5722617690700105 1101 72-73 0.44522045266668187 1101 74-75 0.42321140774014054 1101 76-77 0.4196237586259244 1101 78-79 0.5295259153173291 1101 80-81 0.4784103467841945 1101 82-83 0.5439930849474877 1101 84-85 0.4585046173702665 1101 86-87 0.5281686198878717 1101 88-89 0.7276482976980354 1101 90-91 0.003575101129026148 1101 92-93 -0.18959067010443098 1101 94-95 -0.026905545789630114 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.16092069870141756 1102 2 0.2777556678862467 1102 3 0.2695307249348389 1102 4 0.13878095063925144 1102 5 0.25886788158386054 1102 6 0.1634235349360864 1102 7 0.15633105009170123 1102 8 0.21664882134034968 1102 9 0.26742129535653447 1102 10-11 0.33772424940907086 1102 12-13 0.2866714202663019 1102 14-15 0.35627957972486257 1102 16-17 0.25894395882389887 1102 18-19 0.32090982356194786 1102 20-21 0.3200739659369276 1102 22-23 0.34752489649699214 1102 24-25 0.3525176029069854 1102 26-27 0.3252506085601041 1102 28-29 0.2913786995846337 1102 30-31 0.40555357765301636 1102 32-33 0.31048582066723895 1102 34-35 0.3273816890391643 1102 36-37 0.2510732373546176 1102 38-39 0.29647958610011926 1102 40-41 0.21725458997764946 1102 42-43 0.17849123111390952 1102 44-45 0.21943496592520972 1102 46-47 0.26362470384086123 1102 48-49 0.18428124238754862 1102 50-51 0.19127268761260297 1102 52-53 0.17984907214774637 1102 54-55 0.2635951170234705 1102 56-57 0.14516692289084432 1102 58-59 0.21011747774075928 1102 60-61 0.20059098156020383 1102 62-63 0.09583513603139693 1102 64-65 0.2405334931824754 1102 66-67 0.2603988428917887 1102 68-69 0.20997295098985802 1102 70-71 0.31545483837693666 1102 72-73 0.24640590209397573 1102 74-75 0.2416962893165291 1102 76-77 0.17326382851526034 1102 78-79 0.18082238687625107 1102 80-81 0.2583590217987535 1102 82-83 0.15599803544253632 1102 84-85 0.12308734402527932 1102 86-87 0.22354330441514492 1102 88-89 0.8679365912332742 1102 90-91 0.09562943523890777 1102 92-93 -0.08866063224770393 1102 94-95 0.02983404361048647 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.031662715367914984 1103 2 0.07737729174314367 1103 3 0.08555898647220772 1103 4 0.0042521337957524 1103 5 0.12110174816861274 1103 6 0.11979212823933238 1103 7 0.04756193929040364 1103 8 0.08376524658669382 1103 9 0.07308630398150484 1103 10-11 0.15941633806900768 1103 12-13 0.12367799532715651 1103 14-15 0.14801467507210475 1103 16-17 0.09982512331266946 1103 18-19 0.12938902321221946 1103 20-21 0.17906460843794036 1103 22-23 0.1523539921750654 1103 24-25 0.16574201156503676 1103 26-27 0.17411282175039844 1103 28-29 0.15228189909313272 1103 30-31 0.12842775946260332 1103 32-33 0.25119064592303175 1103 34-35 0.24759557051084613 1103 36-37 0.1927491098941374 1103 38-39 0.24868264500982917 1103 40-41 0.20446334963642698 1103 42-43 0.3419602107684412 1103 44-45 0.26414375512155175 1103 46-47 0.15192472865240347 1103 48-49 0.08746007459058092 1103 50-51 0.1584151059117076 1103 52-53 0.067417303911256 1103 54-55 0.09722702585874288 1103 56-57 0.10372572626521759 1103 58-59 0.11446821263525209 1103 60-61 0.2732533790648688 1103 62-63 0.20208690828499698 1103 64-65 0.22117974545687247 1103 66-67 0.21898275319212246 1103 68-69 0.27423412800254 1103 70-71 0.26438587408193825 1103 72-73 0.22432657602294626 1103 74-75 0.2851763160184859 1103 76-77 0.38043732032745226 1103 78-79 0.34979052175656733 1103 80-81 0.34224273833270047 1103 82-83 0.3194256625131189 1103 84-85 0.2719559077909679 1103 86-87 0.2515456496974089 1103 88-89 1.0293689561284722 1103 90-91 -0.49521037666591017 1103 92-93 -0.28977449363384267 1103 94-95 -0.10135407520139461 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.02630345428599057 1104 2 0.06643546703422132 1104 3 0.13223561878536572 1104 4 0.12458589287363964 1104 5 0.09707254531603127 1104 6 0.2737748784733043 1104 7 0.16121368972634542 1104 8 0.2128725199914996 1104 9 0.25745171228380315 1104 10-11 0.22807653702162156 1104 12-13 0.19932564382334306 1104 14-15 0.21891145524853073 1104 16-17 0.2625756986367378 1104 18-19 0.268860663788999 1104 20-21 0.2737036405849693 1104 22-23 0.03969216894533645 1104 24-25 0.2964863628640302 1104 26-27 0.24408225632559777 1104 28-29 0.3033986962803752 1104 30-31 0.29458120955803935 1104 32-33 0.28680206820379084 1104 34-35 0.3461765326176831 1104 36-37 0.3701775606296209 1104 38-39 0.3462046979140396 1104 40-41 0.3423423594816484 1104 42-43 0.3040115788876676 1104 44-45 0.3274582049296839 1104 46-47 0.27895080811397577 1104 48-49 0.26089148088458103 1104 50-51 0.2478847552555976 1104 52-53 0.16520852883239456 1104 54-55 0.28247035451741453 1104 56-57 0.16313238153444587 1104 58-59 0.1323651293533601 1104 60-61 0.12108656210753566 1104 62-63 0.016651923461040496 1104 64-65 0.025345902462738934 1104 66-67 0.18412124343934977 1104 68-69 0.2239330962594508 1104 70-71 0.2163320908816928 1104 72-73 0.2611128257064834 1104 74-75 0.18144695016926704 1104 76-77 0.24640459014803895 1104 78-79 0.3479937896541898 1104 80-81 0.23812188187498862 1104 82-83 0.2926894496673924 1104 84-85 0.3851873658570213 1104 86-87 0.3717930034911312 1104 88-89 0.49241771857723293 1104 90-91 -0.5586250992729465 1104 92-93 -0.28977449363384267 1104 94-95 -0.10135407520139461 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.24643188849051967 1105 2 -0.3309917292611999 1105 3 -0.22843484490861599 1105 4 -0.08434731561443698 1105 5 -0.21221725417048276 1105 6 -0.08030315539551225 1105 7 -0.1706756839736201 1105 8 -0.25106130643868596 1105 9 -0.1948340800052506 1105 10-11 -0.12776402832907507 1105 12-13 -0.1951446820381193 1105 14-15 -0.3597742202948595 1105 16-17 -0.14755055706002906 1105 18-19 -0.06055362484792681 1105 20-21 -0.13773879688592672 1105 22-23 -0.2761588141411977 1105 24-25 -0.16230143721318768 1105 26-27 -0.17630602777136772 1105 28-29 -0.2370580083216538 1105 30-31 -0.2998176514734183 1105 32-33 -0.24868194804285082 1105 34-35 -0.04370950070370583 1105 36-37 -0.34431206457627184 1105 38-39 -0.3213518752094515 1105 40-41 -0.22181364681087956 1105 42-43 -0.2015406483921396 1105 44-45 -0.2058354334785264 1105 46-47 -0.20948634298438407 1105 48-49 -0.195622850833729 1105 50-51 -0.22177463645196838 1105 52-53 -0.20955869551829664 1105 54-55 -0.1678615886207382 1105 56-57 -0.20977298674159073 1105 58-59 -0.2568083503868621 1105 60-61 -0.3557488132277058 1105 62-63 -0.3440340760655545 1105 64-65 -0.2836195646980002 1105 66-67 -0.3529053587937625 1105 68-69 -0.2676695243736802 1105 70-71 -0.36952206221293693 1105 72-73 -0.425776781903771 1105 74-75 -0.39153550376612145 1105 76-77 -0.3597836985573011 1105 78-79 -0.43271164886476043 1105 80-81 -0.4397311079523547 1105 82-83 -0.36181754774143826 1105 84-85 -0.43833290718261253 1105 86-87 -0.2854997956329939 1105 88-89 1.0083878562631163 1105 90-91 -0.700162643969013 1105 92-93 -0.28977449363384267 1105 94-95 -0.10135407520139461 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.17521995326837825 1106 2 -0.10025827277528165 1106 3 0.008752645423307115 1106 4 -0.1265814672038914 1106 5 -0.052460206080098715 1106 6 -0.05920853448890995 1106 7 -0.09521228993224895 1106 8 -0.02822498460923839 1106 9 -0.1219624790515681 1106 10-11 -0.11116737101362162 1106 12-13 -0.042669504669461844 1106 14-15 -0.09829203799909436 1106 16-17 -0.13367443419086555 1106 18-19 -0.0729562080582582 1106 20-21 -0.12364261611309502 1106 22-23 -0.15981405077081234 1106 24-25 -0.21924753250775098 1106 26-27 -0.24646058726135323 1106 28-29 -0.10956114951100204 1106 30-31 -0.19682306759244028 1106 32-33 -0.15873066018016857 1106 34-35 -0.04255132747372414 1106 36-37 -0.009800598112406078 1106 38-39 -0.022308154045269646 1106 40-41 -0.03455335274166771 1106 42-43 -0.42411399853170906 1106 44-45 -0.07840593933226359 1106 46-47 -0.20093452358955233 1106 48-49 -0.17247136217444137 1106 50-51 -0.2342435863316581 1106 52-53 -0.19877735523093065 1106 54-55 -0.20252993990300894 1106 56-57 -0.36229606787160407 1106 58-59 -0.2000868039736048 1106 60-61 -0.040361925485186134 1106 62-63 -0.21134065714416295 1106 64-65 -0.08667971685232345 1106 66-67 -0.04345254948765387 1106 68-69 -0.017911288669203884 1106 70-71 -0.15452698731840542 1106 72-73 -0.1266607061000613 1106 74-75 -0.2001784401070097 1106 76-77 -0.21697560002725425 1106 78-79 -0.23319298186675752 1106 80-81 -0.16947155938542835 1106 82-83 -0.033892349464778704 1106 84-85 -0.1729041535486644 1106 86-87 -0.16629078653227936 1106 88-89 0.8281040464370548 1106 90-91 -0.22514844748973983 1106 92-93 -0.28182446524088434 1106 94-95 -0.10135407520139461 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 2.303053377552544E-4 1107 2 -0.07747580120660658 1107 3 0.026532776491343668 1107 4 0.039726450594656626 1107 5 -0.08838593163529396 1107 6 0.011770024990504169 1107 7 -0.05244827378663075 1107 8 0.005555921509824202 1107 9 -0.02305234660225608 1107 10-11 -0.06097178573498496 1107 12-13 -0.06166113258357342 1107 14-15 0.01977135119931006 1107 16-17 0.02396547786769787 1107 18-19 0.056849971630803964 1107 20-21 0.0713589761947162 1107 22-23 0.1462577258610125 1107 24-25 0.08913788655696919 1107 26-27 -0.022714002090154395 1107 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112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18298.0 3 3031.0 4 2021.0 5 1685.0 6 1883.0 7 1768.0 8 2018.0 9 2325.0 10 2810.0 11 3334.0 12 4058.0 13 4372.0 14 4785.0 15 5209.0 16 5798.0 17 6746.0 18 8474.0 19 10637.0 20 12136.0 21 16319.0 22 19713.0 23 25567.0 24 38562.0 25 64469.0 26 131650.0 27 362225.0 28 405776.0 29 13579.0 30 472.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.27596378801749 19.498355541993014 14.627199674499034 23.598480995490455 2 14.236707416518184 20.34859178210386 41.9313768205622 23.483323980815754 3 19.053853635538022 25.42803333194832 28.50361531397308 27.014497718540582 4 12.067687663240731 17.45463179675045 38.61470099386045 31.862979546148367 5 13.645306328049076 38.080486692118384 34.34888647299161 13.925320506840928 6 30.69313057335639 37.09312379208626 18.895670159020785 13.318075475536567 7 28.299511748550504 31.99403248228393 21.716424236259453 17.990031532906116 8 25.216068219577526 36.46017699115045 20.45180212253755 17.87195266673448 9 25.78077848981114 18.07556030244465 20.653375377208153 35.49028583053606 10-11 24.22913258343152 27.391816632901307 28.089996694102787 20.289054089564384 12-13 25.2418629529447 24.41050540943988 28.19707094279294 22.150560694822484 14-15 21.986285723004496 26.477522834877277 25.87687157837096 25.65931986374727 16-17 21.97458708247331 29.306739339586258 26.85546932945669 21.863204248483743 18-19 22.160348276188003 28.407092511816117 27.881465381131594 21.551093830864286 20-21 22.982527672430308 27.707142699717046 27.575201704848883 21.73512792300376 22-23 22.487065693603903 27.84223091832198 27.40898940122225 22.26171398685187 24-25 22.276514766215712 27.738955006272676 27.782651815685078 22.201878411826534 26-27 21.922744380022376 28.15460448241956 27.58298579323907 22.33966534431899 28-29 21.83431062360797 28.39322459168904 27.341353683010404 22.43111110169258 30-31 22.162674193876512 27.939553453361814 27.497117960193947 22.400654392567727 32-33 21.864171012389548 28.078738395394097 27.464474139275612 22.592616452940735 34-35 22.31927460630422 28.25603237383849 27.364124307673528 22.06056871218376 36-37 22.274414500402216 27.799755704536423 27.47255914332142 22.45327065173995 38-39 22.097785915301937 28.118790899535483 27.366832807784895 22.41659037737768 40-41 22.618799376123146 27.798418268741738 27.415446038042923 22.16733631709219 42-43 22.088970264130474 27.96358457939172 27.41769233377412 22.529752822703692 44-45 22.060950159275354 28.044922600146815 27.506107391766506 22.38801984881133 46-47 22.438036144169804 27.997660461804497 27.111221306750753 22.453082087274947 48-49 22.239688739041338 27.98831076083893 27.276403037167796 22.495597462951935 50-51 22.21249297502202 28.204682640527107 27.274630653570892 22.30819373087998 52-53 22.313769369002813 28.023259756552406 27.359161495948193 22.30380937849659 54-55 22.1061005686608 28.0019988107161 27.53967846951114 22.352222151111956 56-57 22.191070762553828 28.048816668361987 27.29461228088021 22.46550028820398 58-59 22.23095310751704 27.852880344488522 27.241294544468182 22.67487200352626 60-61 22.023954836740923 27.948920082731494 27.646263520157326 22.380861560370256 62-63 22.113891431865188 27.90412131692266 27.679618892618585 22.302368358593565 64-65 21.972883396060084 27.968161936730752 27.48164818770556 22.577306479503612 66-67 22.0286169599566 28.1647763876174 27.281897399382903 22.524709253043095 68-69 22.4170098277724 27.724888490300227 27.67296930283339 22.185132379093986 70-71 22.3586529006883 27.66944698742074 27.454184043671383 22.517716068219578 72-73 22.236674804190827 27.880598786152643 27.468679008578306 22.41404740107822 74-75 22.196834842165938 28.164988302309023 27.420743905333467 22.217432950191572 76-77 22.304021293188214 27.700937510595736 27.563998236869764 22.431042959346286 78-79 22.38988027626988 27.69458920621573 27.45597245438733 22.45955806312706 80-81 22.31605804767233 27.91102973586953 27.409215746109243 22.363696470348895 82-83 22.392560550846774 27.96057678533132 27.553104107700953 22.09375855612095 84-85 22.373698843793445 28.020674397314615 27.508222290034922 22.097404468857018 86-87 22.270044790744826 28.047109962804356 27.32938998464027 22.353455261810552 88-89 31.50276185617331 17.752432813573 32.99468435291441 17.750120977339275 90-91 30.35675576217049 13.522449249487536 15.383421163847752 40.73737382449422 92-93 22.77127400872036 16.53320193065238 33.57978446547267 27.115739595154597 94-95 14.280163619595957 6.301980589561918 25.459479424305968 53.95837636653616 96-97 24.660855063799623 6.737588944084772 13.519912044753893 55.08164394736171 98-99 57.44526923100306 14.054606139086982 18.488999130630386 10.01112549927958 100-101 30.62145634710529 6.681001988127338 11.746957323636975 50.95058434113039 102-103 22.499650054410893 1.8540343082140132 36.003756835227556 39.64255880214754 104-105 41.86158831804188 4.652450938173909 37.88817568195137 15.597785061832848 106-107 4.346044694237527 1.3321697584733405 5.321271660779348 89.00051388650978 108-109 17.211122697860716 9.452460632927409 18.716430544223325 54.61998612498855 110-111 39.34770157789635 2.4289263810947865 22.620439492141177 35.602932548867685 112-113 26.900118182397193 2.69431096831721 36.7692329467047 33.636337902580905 114-115 26.45876190752134 3.62368530340638 21.57631598432827 48.341236804744014 116-117 17.619611085405968 3.688772246536759 18.205561918631847 60.48605474942542 118-119 18.18638336643548 3.9391802595949863 13.567168285689801 64.30726808827973 120-121 23.806176129579317 3.976889265751813 27.774629241871008 44.44230536279786 122-123 32.91727512258232 1.4399587681638335 31.876136198291473 33.76662991096238 124-125 37.01138063054661 1.6068867057550857 17.806566583131843 43.575166080566454 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 8.0 1 28.0 2 76.5 3 126.5 4 155.5 5 163.5 6 168.0 7 164.5 8 150.0 9 135.0 10 129.5 11 126.0 12 126.0 13 125.5 14 142.0 15 179.0 16 219.0 17 269.5 18 386.5 19 568.5 20 834.5 21 1306.0 22 1991.5 23 2955.5 24 4216.0 25 5865.5 26 7842.0 27 9934.0 28 12375.0 29 15162.0 30 18099.0 31 21267.0 32 24495.0 33 27726.0 34 31174.5 35 34105.5 36 36561.5 37 38811.5 38 40534.0 39 42144.5 40 43616.0 41 44720.0 42 45698.5 43 46333.0 44 46795.0 45 47128.0 46 46621.0 47 45431.0 48 44026.5 49 42738.0 50 41328.5 51 39493.0 52 37259.5 53 34482.5 54 30897.5 55 27350.0 56 24287.5 57 21255.0 58 18169.0 59 15207.5 60 12472.0 61 10028.5 62 8042.5 63 6346.5 64 4789.0 65 3591.0 66 2674.5 67 1978.0 68 1525.0 69 1119.5 70 849.5 71 638.5 72 466.5 73 338.0 74 261.0 75 214.0 76 183.0 77 145.0 78 89.5 79 53.5 80 39.0 81 30.0 82 26.5 83 24.5 84 19.5 85 14.5 86 15.0 87 13.5 88 6.5 89 5.0 90 5.0 91 4.0 92 4.5 93 4.0 94 5.0 95 4.0 96 2.5 97 4.5 98 5.0 99 2.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 1.6953175329739262E-4 3 0.03653409283558811 4 0.040687620791374224 5 0.04187434306445598 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.47658766486963E-4 12-13 0.001949615162920015 14-15 4.2382938324348154E-5 16-17 4.238293832434815E-4 18-19 0.006908418946868749 20-21 0.002203912792866104 22-23 7.205099515139185E-4 24-25 0.0 26-27 0.0 28-29 0.0010595734581087036 30-31 0.0 32-33 0.001356254026379141 34-35 1.6953175329739262E-4 36-37 8.476587664869631E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.6953175329739262E-4 46-47 0.0 48-49 2.1191469162174076E-4 50-51 8.476587664869631E-5 52-53 0.0 54-55 0.0015681687180008816 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.6953175329739262E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.3906350659478523E-4 80-81 0.0 82-83 7.628928898382667E-4 84-85 0.0 86-87 0.0010171905197843556 88-89 19.334715017122708 90-91 26.289924727901536 92-93 40.39416132641644 94-95 44.80516563252297 96-97 48.459253043094975 98-99 93.02855762384294 100-101 51.650730681856714 102-103 53.06873664937443 104-105 74.90247685891568 106-107 81.69048587800495 108-109 12.576670735428747 110-111 39.17192215101888 112-113 52.087953073610684 114-115 86.834884548876 116-117 66.47416336079748 118-119 71.81055674227783 120-121 80.40653714440714 122-123 75.82337334282711 124-125 50.00728986539179 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1179720.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.83430816394596 #Duplication Level Percentage of deduplicated Percentage of total 1 80.75673579084672 55.588340377415065 2 12.319689523467876 16.960346102850487 3 3.511287216651518 7.250910789693439 4 1.3316516808778605 3.6665328867433304 5 0.6307385347669046 2.1708225336510423 6 0.36609323624184054 1.511986478412427 7 0.23448584329243558 1.1298469558091975 8 0.15334822490065111 0.8444495175364408 9 0.12034731323381652 0.7455621641255512 >10 0.5334846897801978 6.464995512151734 >50 0.027991318227498056 1.3015532530485017 >100 0.012670465733766715 1.6996968019390923 >500 0.0014761619789411245 0.6649566266238285 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.47658766486963E-5 2 0.0 0.0 0.0 0.0 8.47658766486963E-5 3 0.0 0.0 0.0 0.0 8.47658766486963E-5 4 0.0 0.0 0.0 0.0 8.47658766486963E-5 5 0.0 0.0 0.0 0.0 8.47658766486963E-5 6 0.0 0.0 0.0 0.0 8.47658766486963E-5 7 0.0 0.0 0.0 0.0 8.47658766486963E-5 8 0.0 0.0 0.0 0.0 8.47658766486963E-5 9 0.0 0.0 0.0 0.0 8.47658766486963E-5 10-11 0.0 0.0 0.0 0.0 8.47658766486963E-5 12-13 0.0 0.0 0.0 0.0 8.47658766486963E-5 14-15 0.0 0.0 0.0 0.0 8.47658766486963E-5 16-17 0.0 0.0 0.0 8.47658766486963E-5 8.47658766486963E-5 18-19 0.0 0.0 0.0 8.47658766486963E-5 8.47658766486963E-5 20-21 0.0 0.0 0.0 8.47658766486963E-5 8.47658766486963E-5 22-23 0.0 0.0 0.0 1.695317532973926E-4 8.47658766486963E-5 24-25 0.0 0.0 0.0 1.695317532973926E-4 8.47658766486963E-5 26-27 0.0 0.0 0.0 2.96680568270437E-4 8.47658766486963E-5 28-29 0.0 0.0 0.0 7.628928898382667E-4 8.47658766486963E-5 30-31 0.0 0.0 0.0 0.0024158274844878445 8.47658766486963E-5 32-33 0.0 0.0 0.0 0.00639982368697657 8.47658766486963E-5 34-35 0.0 0.0 0.0 0.011061946902654867 8.47658766486963E-5 36-37 0.0 0.0 0.0 0.01852134404774014 8.47658766486963E-5 38-39 0.0 0.0 0.0 0.03191435255823416 8.47658766486963E-5 40-41 0.0 0.0 0.0 0.05115620655748822 8.47658766486963E-5 42-43 0.0 0.0 0.0 0.07111857050825621 8.47658766486963E-5 44-45 0.0 0.0 0.0 0.0904875733224833 8.47658766486963E-5 46-47 0.0 0.0 0.0 0.11430678466076696 8.47658766486963E-5 48-49 0.0 0.0 0.0 0.14215237513986367 8.47658766486963E-5 50-51 0.0 0.0 0.0 0.1758891940460448 8.47658766486963E-5 52-53 0.0 0.0 0.0 0.20924456650730683 8.47658766486963E-5 54-55 0.0 0.0 0.0 0.246032956972841 8.47658766486963E-5 56-57 0.0 0.0 0.0 0.2858305360594039 8.47658766486963E-5 58-59 0.0 0.0 0.0 0.33147696063472687 8.47658766486963E-5 60-61 0.0 0.0 0.0 0.38555758993659517 8.47658766486963E-5 62-63 0.0 0.0 0.0 0.4406554097582477 8.47658766486963E-5 64-65 0.0 0.0 0.0 0.49766046180449597 8.47658766486963E-5 66-67 0.0 0.0 0.0 0.5651764825551826 8.47658766486963E-5 68-69 0.0 0.0 0.0 0.6442206625300919 8.47658766486963E-5 70-71 0.0 0.0 0.0 0.7283084121655986 8.47658766486963E-5 72-73 0.0 0.0 0.0 0.8155324992371071 8.47658766486963E-5 74-75 0.0 0.0 0.0 0.9070372630793748 8.47658766486963E-5 76-77 0.0 0.0 0.0 1.003839894212186 8.47658766486963E-5 78-79 0.0 0.0 0.0 1.0314311870613366 8.47658766486963E-5 80-81 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 82-83 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 84-85 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 86-87 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 88-89 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 90-91 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 92-93 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 94-95 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 96-97 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 98-99 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 100-101 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 102-103 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 104-105 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 106-107 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 108-109 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 110-111 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 112-113 0.0 0.0 0.0 1.0320245481978774 8.47658766486963E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 430 0.0 1958.2391 108-109 CCGCGCC 480 0.0 1186.7028 108-109 CTACGCG 25 0.008321522 990.63873 108-109 CGTCGCT 65 2.6318008E-5 936.4704 110-111 CCGCTCG 140 0.0 884.49884 108-109 CGTCGCC 225 0.0 880.56775 108-109 CCGCGCG 155 1.8189894E-12 798.9022 108-109 CTCCGCC 275 0.0 774.7164 110-111 CTATGCG 65 4.890963E-5 762.0298 108-109 CCTCGCC 690 0.0 753.7469 108-109 TTGTTCG 245 0.0 707.5991 108-109 TTGTGCG 140 2.9905823E-7 652.18475 110-111 CGCCGCC 455 0.0 602.01666 110-111 AAGTTCG 125 4.3479668E-7 594.38324 108-109 CCGTGCG 85 1.0930752E-4 582.7287 108-109 CGGCGCG 90 1.297345E-4 550.35486 108-109 CCGCGCT 95 1.525575E-4 521.3888 108-109 CGTGTCT 180 8.1636244E-7 507.2548 110-111 CAGCGCG 200 4.5456545E-9 495.31937 108-109 CCGCACG 100 1.779088E-4 495.31937 108-109 >>END_MODULE