FastQCFastQC Report
Fri 27 May 2016
SRR939991_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939991_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179720
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35100.297528227036924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22760.1929271352524328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16260.1378293154307802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47950.046.4521261
GGTATCA30150.043.456821
ATCAACG58550.037.223873
TCAACGC58600.037.192114
CAACGCA59500.036.629545
TATCAAC60550.036.5844152
AACGCAG62250.035.075786
ACGCAGA70100.031.147897
CGCAGAG70300.031.143918
TACATGG45900.029.9686132
GTACATG48200.029.7778021
ACATGGG46550.028.270923
GCAGAGT81550.026.7745849
GAGTACT45900.025.85894612-13
AGAGTAC70150.025.27372210-11
GGACCGA2651.380613E-924.6960476
GTACTTT50450.023.41082814-15
GTAGGAC8650.023.4061583
GTACCTG11600.022.5900551
CATGGGG24350.022.4986364
Produced by FastQC (version 0.11.5)