FastQCFastQC Report
Fri 27 May 2016
SRR939990_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939990_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597355
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC6630.11098927773267153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTC52.0154672E-46355.236110-111
AACGTCG53.8038893E-44629.8325108-109
CGTCGCA53.8038893E-44629.8325108-109
CGTTGCA108.0610334E-43177.618110-111
CAGCGCG157.325052E-73086.5552108-109
CCGCTCC750.02777.8997108-109
CGGCGCG100.00152133862314.9163108-109
CCGCGCG201.7360308E-62314.9163108-109
CGCTGGT150.00181354442118.412110-111
GTCCGCG150.00181354442118.412110-111
CGGTGCC150.00181354442118.412110-111
CCGTGCG253.3901433E-61851.933108-109
CCGTTCG253.3901433E-61851.933108-109
CCGCTCG405.9135346E-91736.1871108-109
CCGCGCC700.01653.5116108-109
TTCGTCG200.00322374541588.809110-111
CGGCTCG200.00322374541588.809110-111
CCGCTAG150.0034225231543.2776108-109
CCGTCGC150.0034225231543.2776108-109
CATAGCG150.0034225231543.2776108-109
Produced by FastQC (version 0.11.5)