FastQCFastQC Report
Fri 27 May 2016
SRR939990_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939990_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences597355
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.5902687681529408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.4310669534866202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14710.24625222857429835No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9650.16154547965615088No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6690.11199370558545589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG6620.1108218730905408No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC6150.10295385491039667No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG304.2383326E-579.424465
GGTATCA32700.048.824631
GTATCAA55750.048.6203961
TATCAAC69000.039.1079142
ATCAACG69450.038.7687453
TCAACGC70100.038.4092644
CAACGCA71650.037.5783585
AACGCAG73150.036.761576
ATGGGCG1209.1955553E-734.7482035
TACCTGG5950.034.0390552
ACATGCG700.00278940133.9963159
ACGCAGA83150.032.26897
CGCAGAG83350.032.1914758
GTACACG750.003885490431.7724461
GCAGAGT88300.030.2521029
TACATGG65950.030.1680492
AGTACTT46150.029.84346412-13
GTACATG67750.029.720721
GAGTACT46950.028.51128812-13
ACATGGG65950.028.361583
Produced by FastQC (version 0.11.5)