##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939990_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 597355 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.85282620887077 34.0 31.0 34.0 31.0 34.0 2 32.913451800018414 34.0 33.0 34.0 31.0 34.0 3 32.95288396347231 34.0 33.0 34.0 31.0 34.0 4 36.35597592721246 37.0 37.0 37.0 35.0 37.0 5 36.27801223727934 37.0 37.0 37.0 35.0 37.0 6 36.42838178302684 37.0 37.0 37.0 35.0 37.0 7 36.42765859497284 37.0 37.0 37.0 35.0 37.0 8 36.45962283734128 37.0 37.0 37.0 35.0 37.0 9 38.29429066468013 39.0 39.0 39.0 37.0 39.0 10-11 38.29045626135213 39.0 39.0 39.0 37.0 39.0 12-13 38.27423642557608 39.0 39.0 39.0 37.0 39.0 14-15 39.74943375379799 41.0 40.0 41.0 37.0 41.0 16-17 39.78006294414544 41.0 40.0 41.0 37.5 41.0 18-19 39.89079107063639 41.0 40.0 41.0 38.0 41.0 20-21 39.92798001188573 41.0 40.0 41.0 38.0 41.0 22-23 39.86744816733768 41.0 40.0 41.0 38.0 41.0 24-25 39.82991186145591 41.0 40.0 41.0 37.0 41.0 26-27 39.72225979526412 41.0 40.0 41.0 37.0 41.0 28-29 39.62380996225025 41.0 40.0 41.0 37.0 41.0 30-31 39.41039666529953 41.0 39.0 41.0 36.0 41.0 32-33 39.1508876631149 41.0 39.0 41.0 35.0 41.0 34-35 39.01192088456613 41.0 39.0 41.0 35.0 41.0 36-37 38.96017610968352 41.0 39.0 41.0 35.0 41.0 38-39 38.969271203890486 41.0 39.0 41.0 35.0 41.0 40-41 38.833788952967666 41.0 39.0 41.0 35.0 41.0 42-43 38.63037473529141 41.0 39.0 41.0 35.0 41.0 44-45 38.2878296825171 41.0 39.0 41.0 35.0 41.0 46-47 37.78896468599074 41.0 39.0 41.0 33.5 41.0 48-49 37.321762603477 41.0 39.0 41.0 33.0 41.0 50-51 37.073796151367276 41.0 39.0 41.0 32.0 41.0 52-53 36.95226707736606 41.0 39.0 41.0 31.0 41.0 54-55 36.85796218329134 41.0 38.5 41.0 31.0 41.0 56-57 36.738364121837094 41.0 38.0 41.0 31.0 41.0 58-59 36.61027697098041 41.0 38.0 41.0 31.0 41.0 60-61 36.45985804086347 40.0 38.0 41.0 30.0 41.0 62-63 36.27710406709578 40.0 37.0 41.0 29.5 41.0 64-65 36.08272718902495 40.0 37.0 41.0 29.0 41.0 66-67 35.8455767508433 39.5 36.0 41.0 29.0 41.0 68-69 35.5510215868286 39.0 36.0 41.0 28.5 41.0 70-71 35.21982907986038 39.0 35.0 41.0 28.0 41.0 72-73 34.88097027730579 37.5 35.0 40.5 28.0 41.0 74-75 34.52584141758251 37.0 35.0 39.5 28.0 41.0 76-77 34.1308769492178 37.0 35.0 39.0 27.5 41.0 78-79 33.7493709770572 36.0 35.0 39.0 26.5 40.5 80-81 33.43496915569469 36.0 35.0 38.0 26.5 39.5 82-83 33.15754032359317 35.5 35.0 37.0 26.5 39.0 84-85 32.846758627616744 35.0 35.0 37.0 26.0 39.0 86-87 32.59174025495727 35.0 35.0 36.5 26.0 37.5 88-89 32.35289065965799 35.0 35.0 36.0 26.0 37.0 90-91 32.155542349189346 35.0 34.0 36.0 26.0 37.0 92-93 31.995974755379965 35.0 34.0 35.5 26.0 36.5 94-95 31.878927940671794 35.0 34.0 35.0 25.0 36.0 96-97 31.774700973457996 35.0 34.0 35.0 25.0 36.0 98-99 31.677262264482593 35.0 34.0 35.0 25.0 36.0 100-101 31.62952934184865 35.0 34.0 35.0 25.0 36.0 102-103 30.85429518460547 34.5 32.5 35.0 21.5 35.0 104-105 31.46083986908957 35.0 34.0 35.0 24.0 35.0 106-107 31.523303563207808 35.0 34.0 35.0 24.0 35.0 108-109 31.518026131864637 35.0 34.0 35.0 24.0 35.0 110-111 31.488644106101063 35.0 34.0 35.0 24.0 35.0 112-113 31.42992525382729 35.0 34.0 35.0 23.0 35.0 114-115 31.379775008160976 35.0 34.0 35.0 21.5 35.0 116-117 31.32563467284948 35.0 34.0 35.0 19.5 35.0 118-119 31.261309439110747 35.0 34.0 35.0 19.0 35.0 120-121 31.16910798436441 35.0 34.0 35.0 13.0 35.0 122-123 31.025133295946297 35.0 34.0 35.0 2.0 35.0 124-125 29.12895849201898 33.5 29.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08671798909254136 1101 2 0.07015042419332218 1101 3 0.0765211912592676 1101 4 -0.03216881591013987 1101 5 -0.013583605117069908 1101 6 0.009309608489587617 1101 7 0.05548377125078474 1101 8 0.06326821161010088 1101 9 0.10774028331481844 1101 10-11 0.08438646550219175 1101 12-13 0.05626378715899705 1101 14-15 0.1875939502127224 1101 16-17 0.11700756303714144 1101 18-19 0.010384631119912058 1101 20-21 -0.03785377306113702 1101 22-23 0.04794066928400298 1101 24-25 0.12936431293645967 1101 26-27 0.09583659376242792 1101 28-29 0.1643608089653199 1101 30-31 0.2587176001057685 1101 32-33 0.29247988833449057 1101 34-35 0.3693942781883308 1101 36-37 0.18676926858302778 1101 38-39 0.13892177182857068 1101 40-41 0.35785679736096654 1101 42-43 0.35417196849254395 1101 44-45 0.4220937624553187 1101 46-47 0.40977852306178164 1101 48-49 0.2774720438524483 1101 50-51 0.3665372995882805 1101 52-53 0.22358143957991672 1101 54-55 0.25812333514524965 1101 56-57 0.3663980185247695 1101 58-59 0.26461899036632275 1101 60-61 0.3635915401023766 1101 62-63 0.3432670421479713 1101 64-65 0.42431839849701447 1101 66-67 0.43321121429568166 1101 68-69 0.29431799673444914 1101 70-71 0.42666093686042217 1101 72-73 0.40162190362916306 1101 74-75 0.4299050824113735 1101 76-77 0.49215821189383746 1101 78-79 0.42201243142376654 1101 80-81 0.397681218423088 1101 82-83 0.2742268879031329 1101 84-85 0.4459193998279787 1101 86-87 0.3831659372129579 1101 88-89 0.3830153818772999 1101 90-91 0.49003785841838976 1101 92-93 0.44240839584021074 1101 94-95 0.36300769289582746 1101 96-97 0.431471623957588 1101 98-99 0.358138782940582 1101 100-101 0.46745335292466095 1101 102-103 0.4625506725872377 1101 104-105 0.48711884845168996 1101 106-107 0.5201499882197496 1101 108-109 0.469756306394185 1101 110-111 0.4470489169919354 1101 112-113 0.44243926143376555 1101 114-115 0.39440045559569015 1101 116-117 0.37223326017481284 1101 118-119 0.44351110502248403 1101 120-121 0.5622566552577339 1101 122-123 0.4481733646865038 1101 124-125 0.27007202498907645 1102 1 0.055523693192363055 1102 2 0.06829362086593704 1102 3 0.15621519007091678 1102 4 -0.08839282066355736 1102 5 -0.09454023019134183 1102 6 0.02356985804395606 1102 7 -0.10189887877892545 1102 8 -0.13555828868698683 1102 9 -0.07994539701791581 1102 10-11 0.0633674518361147 1102 12-13 -0.05573858954926436 1102 14-15 0.07492312430660064 1102 16-17 0.1335702487174686 1102 18-19 0.08350554615258687 1102 20-21 0.028025608994717288 1102 22-23 0.13264803707961192 1102 24-25 0.12390531115392633 1102 26-27 0.11938085995375758 1102 28-29 0.1832630668381654 1102 30-31 0.07730791501960965 1102 32-33 0.15206842071713567 1102 34-35 0.06725524075518052 1102 36-37 0.17132066489912745 1102 38-39 0.022648747467307828 1102 40-41 0.14774093283334366 1102 42-43 0.14201362030478748 1102 44-45 0.41585490327528163 1102 46-47 0.44654322894412957 1102 48-49 0.22592718348406038 1102 50-51 0.25048709162631155 1102 52-53 0.30995993037017655 1102 54-55 0.3667091937311042 1102 56-57 0.3833692009371319 1102 58-59 0.4036564235214044 1102 60-61 0.3747694961332755 1102 62-63 0.47610275218956133 1102 64-65 0.48243634264436963 1102 66-67 0.5451764549373905 1102 68-69 0.3730093217493078 1102 70-71 0.598081020045214 1102 72-73 0.5040060391015402 1102 74-75 0.4884686593573022 1102 76-77 0.5385040229455313 1102 78-79 0.4686553310078452 1102 80-81 0.39266784943913535 1102 82-83 0.4906558837438979 1102 84-85 0.47284304807515554 1102 86-87 0.49799065497884953 1102 88-89 0.42379078294681705 1102 90-91 0.5088658441581444 1102 92-93 0.44385670243556774 1102 94-95 0.4350516619986209 1102 96-97 0.3671890927632937 1102 98-99 0.44396023273261775 1102 100-101 0.5227860920809242 1102 102-103 0.3802571491172415 1102 104-105 0.4301521223673177 1102 106-107 0.47510393949723095 1102 108-109 0.48776729866988333 1102 110-111 0.5337616323811183 1102 112-113 0.6597595228716742 1102 114-115 0.6309943355719234 1102 116-117 0.5430220302282898 1102 118-119 0.4445509148858271 1102 120-121 0.556426292809725 1102 122-123 0.5819374763917935 1102 124-125 0.7094659643830212 1103 1 0.09796483321714788 1103 2 0.12307470306337365 1103 3 0.14898207828390042 1103 4 0.006886177496483015 1103 5 -0.10930685555275943 1103 6 0.07322064096611314 1103 7 0.0802337861604343 1103 8 0.06887920896612343 1103 9 0.1139311002972434 1103 10-11 0.07014526716412206 1103 12-13 0.044220105204964 1103 14-15 0.15542141577143553 1103 16-17 0.14367595259641774 1103 18-19 0.05198917425517635 1103 20-21 0.12398051117638431 1103 22-23 0.07599648437786755 1103 24-25 0.23664751009500407 1103 26-27 0.24216070299382153 1103 28-29 0.2953246679796919 1103 30-31 0.09188444237495474 1103 32-33 0.16575782404408557 1103 34-35 0.24090624897868196 1103 36-37 0.27326013393897597 1103 38-39 0.25417169065382694 1103 40-41 0.3135204754716199 1103 42-43 0.37952582412739133 1103 44-45 0.21970192366504904 1103 46-47 0.15152520483689358 1103 48-49 0.08007742192798162 1103 50-51 0.12796305948332076 1103 52-53 0.09592835063227056 1103 54-55 0.20044619196624325 1103 56-57 -0.04432549697132515 1103 58-59 -0.1213520252788669 1103 60-61 0.013341555012026163 1103 62-63 0.0021508728008754474 1103 64-65 0.06106885738282841 1103 66-67 0.17356301571243193 1103 68-69 0.10985500910614832 1103 70-71 0.14388290506642676 1103 72-73 0.2242310588154055 1103 74-75 0.10071331783802151 1103 76-77 0.03601162686577197 1103 78-79 0.07734445270811818 1103 80-81 0.01364762864382385 1103 82-83 0.018630800194642916 1103 84-85 0.0380278890499639 1103 86-87 0.04127198125274134 1103 88-89 0.028175710287136724 1103 90-91 0.029685096157692215 1103 92-93 -0.020778027766073848 1103 94-95 -0.037043828219545105 1103 96-97 -0.050090111922980185 1103 98-99 0.0028460237280754086 1103 100-101 0.011727551282607607 1103 102-103 -0.028998042201148877 1103 104-105 0.019366759444409354 1103 106-107 0.08902427669823965 1103 108-109 0.10237762122629945 1103 110-111 0.16710951642603433 1103 112-113 0.1302121908150511 1103 114-115 0.04960657318973816 1103 116-117 0.004967225677201981 1103 118-119 0.027184875307892042 1103 120-121 0.038418613789033884 1103 122-123 0.17650302030678233 1103 124-125 0.08340986434922115 1104 1 0.07582672349539621 1104 2 0.10400069157300607 1104 3 0.09555862441435181 1104 4 0.03155667428593745 1104 5 0.07343956778845495 1104 6 -0.019157414683313334 1104 7 -0.06316150504154905 1104 8 -0.050760308889003625 1104 9 0.023337431264906172 1104 10-11 0.03710482557349337 1104 12-13 0.030524036713366343 1104 14-15 0.09179181117528401 1104 16-17 -0.04880348865626161 1104 18-19 0.07701564716268905 1104 20-21 0.1031266190957254 1104 22-23 0.04964298657456112 1104 24-25 0.08458712933574475 1104 26-27 0.09705762763052661 1104 28-29 0.1400559961310961 1104 30-31 0.12273720794890863 1104 32-33 0.2146189257676454 1104 34-35 0.0648309983309403 1104 36-37 0.05323985681832255 1104 38-39 0.07373460605317206 1104 40-41 0.1339530540454632 1104 42-43 0.11835200414316915 1104 44-45 0.07433975176012808 1104 46-47 -0.4684567710558625 1104 48-49 -0.22503241247553518 1104 50-51 -0.25342704978783104 1104 52-53 -0.19402996861972355 1104 54-55 -0.13096757394566083 1104 56-57 -0.16721160714367755 1104 58-59 -0.2454344855695041 1104 60-61 -0.24823555437178157 1104 62-63 -0.2661447225579181 1104 64-65 -0.2319828492748215 1104 66-67 -0.43065687839593636 1104 68-69 -0.35741997117998636 1104 70-71 -0.263232111267925 1104 72-73 -0.24728183968634454 1104 74-75 -0.17155659316794214 1104 76-77 -0.2798293103878038 1104 78-79 -0.2232638609113451 1104 80-81 -0.28952912025783917 1104 82-83 -0.20853603544802723 1104 84-85 -0.22324281051070471 1104 86-87 -0.340746718758524 1104 88-89 -0.17908800493196964 1104 90-91 -0.18014930735700574 1104 92-93 -0.26864329756443084 1104 94-95 -0.19217056022360168 1104 96-97 -0.0921290890548967 1104 98-99 -0.23525693898455202 1104 100-101 -0.22670885741402103 1104 102-103 -0.23221759835750433 1104 104-105 -0.11494888773369283 1104 106-107 -0.16022434333105195 1104 108-109 -0.15347007506749222 1104 110-111 -0.2715161453966566 1104 112-113 -0.2068566387444868 1104 114-115 -0.15797031089272195 1104 116-117 -0.14614463643837894 1104 118-119 -0.04307534774043731 1104 120-121 0.019734373617804835 1104 122-123 0.008957678411995573 1104 124-125 -0.050534035616983886 1105 1 -0.026412434552142372 1105 2 0.01032622232967384 1105 3 -0.04649907836191147 1105 4 -0.053400683725307374 1105 5 -0.08419425547410953 1105 6 -0.011006443897613849 1105 7 -0.0808062156203917 1105 8 -0.01027400876198925 1105 9 -0.04300261591345844 1105 10-11 0.047973996322902224 1105 12-13 0.08910505649198797 1105 14-15 0.00972322108274426 1105 16-17 0.0836443429532423 1105 18-19 0.08912234286220411 1105 20-21 0.042095953154877463 1105 22-23 0.06902603865221835 1105 24-25 0.02616216109041858 1105 26-27 0.09419789255522204 1105 28-29 -0.01017808116885277 1105 30-31 -0.025301260568603823 1105 32-33 -8.628379775856843E-4 1105 34-35 0.06496890433709979 1105 36-37 0.06484573596911503 1105 38-39 -0.027005731453634496 1105 40-41 0.10021326090446792 1105 42-43 0.14571908815695878 1105 44-45 0.06981062818909578 1105 46-47 0.1468290209963925 1105 48-49 0.218132167455515 1105 50-51 0.2852410685210103 1105 52-53 0.2918673276967283 1105 54-55 0.17984353111693707 1105 56-57 0.2017377668356417 1105 58-59 0.32945936309679524 1105 60-61 0.4385290981413803 1105 62-63 0.4541383476652783 1105 64-65 0.3579754160127848 1105 66-67 0.26514787573216836 1105 68-69 0.32069652310417496 1105 70-71 0.2292883024501009 1105 72-73 0.24652327735230983 1105 74-75 0.3074708065780172 1105 76-77 0.30301590826579883 1105 78-79 0.2652318748897784 1105 80-81 0.2233360584756099 1105 82-83 0.05418942272653737 1105 84-85 -0.04755781686163374 1105 86-87 -0.1373462469748148 1105 88-89 -0.023174377641097976 1105 90-91 -0.0854296557511276 1105 92-93 0.007386461100061581 1105 94-95 -0.07306694926370128 1105 96-97 0.058286443516326614 1105 98-99 0.004830159545658574 1105 100-101 0.13422382268033317 1105 102-103 0.006240031836650672 1105 104-105 -0.1192021992923955 1105 106-107 -0.05985417457764797 1105 108-109 -0.05837299649735783 1105 110-111 -0.08733178684103748 1105 112-113 -0.13707257051730437 1105 114-115 -0.06363534446194663 1105 116-117 -0.08915679394155163 1105 118-119 -0.06862424993604677 1105 120-121 -0.19402356505031548 1105 122-123 -0.09570572205978678 1105 124-125 -0.23782127931141162 1106 1 -0.02239587370632279 1106 2 0.04273935430238396 1106 3 -0.03818531146415438 1106 4 -0.0039140421592520624 1106 5 -0.01172249366130984 1106 6 -0.019757850596974436 1106 7 -0.06178486157674001 1106 8 -0.006117125313110705 1106 9 0.02385068444637284 1106 10-11 -0.08316164422893735 1106 12-13 0.01322248289208261 1106 14-15 -0.03355727129676467 1106 16-17 -0.0701520086590719 1106 18-19 -0.02170403222984163 1106 20-21 0.0019616046824566524 1106 22-23 -0.026303719464955577 1106 24-25 -0.04691396044840701 1106 26-27 -0.03001331380236394 1106 28-29 -0.08546393637259087 1106 30-31 -0.11364892718432174 1106 32-33 0.03302854399165511 1106 34-35 0.10279626585536761 1106 36-37 -0.18126552179378308 1106 38-39 -0.3221903043032981 1106 40-41 -0.39364449921334455 1106 42-43 -0.4632018171322372 1106 44-45 -0.48771677639848576 1106 46-47 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2216 56-57 -0.9182580649303773 2216 58-59 -0.9567386991827149 2216 60-61 -0.9248641493195535 2216 62-63 -1.00247440306417 2216 64-65 -0.9069702444489778 2216 66-67 -0.9480227270351378 2216 68-69 -1.0753548376717248 2216 70-71 -1.0930407237081141 2216 72-73 -0.9867107639030408 2216 74-75 -1.096962262767164 2216 76-77 -0.9428138244589093 2216 78-79 -0.9375201761863394 2216 80-81 -1.0446585126537826 2216 82-83 -0.87129983644612 2216 84-85 -0.9070263161957328 2216 86-87 -0.8733608052945101 2216 88-89 -0.8653935047518999 2216 90-91 -0.8510244424087148 2216 92-93 -0.8087832368554722 2216 94-95 -0.7972737140024755 2216 96-97 -0.9479669241117428 2216 98-99 -0.925250765192299 2216 100-101 -0.8022426332024644 2216 102-103 -0.7665912156852848 2216 104-105 -0.9094043078088063 2216 106-107 -0.8676004816239953 2216 108-109 -0.7870575628485277 2216 110-111 -0.8282851941181626 2216 112-113 -0.846695091180564 2216 114-115 -0.9013741283542664 2216 116-117 -1.024841451790543 2216 118-119 -1.0319265078988984 2216 120-121 -1.1319397530478312 2216 122-123 -1.2914719146321971 2216 124-125 -1.368220921453272 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52.0 3 1.0 4 6.0 5 30.0 6 76.0 7 209.0 8 353.0 9 716.0 10 1594.0 11 2463.0 12 3207.0 13 5678.0 14 11030.0 15 11602.0 16 3531.0 17 1840.0 18 1272.0 19 1116.0 20 1129.0 21 1022.0 22 1003.0 23 1003.0 24 1212.0 25 1467.0 26 1898.0 27 2420.0 28 2964.0 29 3706.0 30 4743.0 31 6118.0 32 7952.0 33 10616.0 34 15748.0 35 25430.0 36 49532.0 37 192759.0 38 218533.0 39 3323.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47352250256596 19.827607858108006 15.445639379616003 20.253230259710033 2 13.3798160222983 20.864812381247333 45.354772287835544 20.400599308618826 3 18.8912790551682 29.16741301236283 29.45685563860686 22.48445229386211 4 10.771819102543713 17.801307430255044 40.41415908463142 31.01271438256983 5 11.106751840115352 40.222155156514596 34.38207333635129 14.289019667018762 6 27.12725263871567 38.41718910865398 20.446133371278385 14.00942488135196 7 26.066242016891128 34.88177047149518 21.955453624729014 17.096533886884682 8 25.35226121820358 35.958517129680004 20.775083493065264 17.914138159051152 9 28.227268542156676 16.215985469277065 21.833917854542108 33.722828134024155 10-11 23.66842162533167 28.089494521683083 28.454938855454458 19.78714499753078 12-13 26.211632948581666 26.002963062165712 29.328288873450457 18.45711511580216 14-15 21.708866586870453 27.639845652919952 29.22181952105532 21.42946823915427 16-17 18.541905567041375 30.17828594386922 30.995220597467167 20.284587891622234 18-19 18.678005541093654 27.229369470415417 35.521339906755614 18.57128508173532 20-21 21.119252938507717 26.160377816505864 34.56071799868414 18.15965124630228 22-23 19.398547594893483 27.68124093920858 34.54936268946052 18.370848776437423 24-25 18.84993010856191 26.9029304182605 36.076286295418974 18.17085317775862 26-27 18.216805752023504 27.666797800303 35.344728009307694 18.771668438365797 28-29 17.99465979191603 27.787329142637123 34.598103305404656 19.619907760042185 30-31 18.888851687857304 27.305287475621697 35.16133622385349 18.64452461266751 32-33 18.119292547982354 27.6926618175122 35.54820835181759 18.63983728268785 34-35 18.446401218705795 27.93221786040127 35.471034811795334 18.150346109097608 36-37 18.254136987218654 27.524587556812953 35.193645319784714 19.027630136183678 38-39 19.005951235027744 28.174284972922297 34.37235814549137 18.44740564655858 40-41 20.11065446844841 27.09460873350018 34.590821203471975 18.20391559457944 42-43 18.644692017309637 28.03994274761239 34.557675084330086 18.75769015074788 44-45 19.02470055494639 28.335663047936322 33.5421985251651 19.097437871952188 46-47 19.91043851646006 27.891622234684565 32.432389450159455 19.76554979869592 48-49 20.124967565350588 28.509010554862684 31.684174402156174 19.681847477630555 50-51 19.928769324773377 29.481464121000073 31.02150312628169 19.568263427944856 52-53 19.469076177482403 29.32979551522964 31.54966477220413 19.651463535083828 54-55 19.57127671150321 28.773175080144973 31.821195101740173 19.834353106611648 56-57 19.87905014606055 28.899816691916868 30.806806672749037 20.41432648927355 58-59 20.025529207924937 29.095094206962358 30.7600170752735 20.119359509839207 60-61 19.817361535435378 28.59555875484427 31.69539051317893 19.89168919654142 62-63 19.810079433502693 28.409739602079163 31.757079123804104 20.02310184061404 64-65 19.776431100434415 28.913962384176912 31.166057034761575 20.143549480627097 66-67 19.831088716090097 29.27162240208921 30.82957370407882 20.067715177741878 68-69 20.394489039181057 28.441713888726134 31.569920733901952 19.593876338190857 70-71 20.483129797189275 28.25154221526563 31.303245138987705 19.96208284855739 72-73 19.892275112788877 28.636656594487363 31.4168291886734 20.054239104050357 74-75 19.67615571979811 28.815444752282982 31.713219107565855 19.795180420353056 76-77 19.594294849796185 28.116111859781874 32.072218362615196 20.21737492780675 78-79 19.388470842296456 28.159804471377992 31.72033380485641 20.731390881469146 80-81 19.592285994090616 28.78254974010429 31.515179415925203 20.109984849879886 82-83 19.46874136819814 28.81887654744666 31.938294648910613 19.774087435444585 84-85 19.574624804345824 28.389232533418152 31.813912999807485 20.22222966242854 86-87 18.859639577805492 29.170091486636924 31.045274585464256 20.92499435009333 88-89 19.859296398289125 28.23237438374166 31.427040871843374 20.48128834612584 90-91 19.62723758728118 28.54259890750999 31.77913822861266 20.05102527659617 92-93 19.12924475395703 29.01817177390329 31.829732738488836 20.02285073365084 94-95 19.08353945116158 29.23968678460373 31.245872412018027 20.430901352216658 96-97 19.683688928693993 28.73634605887621 31.08260582065941 20.497359191770386 98-99 19.83269426022789 28.434526186093734 31.339107172601633 20.39367238107674 100-101 19.333478417356513 28.454018129922744 31.532422094064668 20.680081358656075 102-103 19.341629921286206 28.71789592101166 31.14225400683682 20.798220150865316 104-105 19.492534839785954 28.30282175271599 30.804573263367775 21.400070144130286 106-107 19.290334407873974 28.300654853137274 30.748548281920847 21.660462457067904 108-109 19.080362598454855 28.578567183668003 30.597550870085627 21.743519347791516 110-111 18.82168886686494 28.805123470379414 30.858512763893298 21.514674898862353 112-113 19.069754204095233 28.26623606552437 30.616209990046954 22.04779974033345 114-115 18.95044439496492 28.442031108484606 30.047762623277958 22.559761873272517 116-117 19.160741877863693 28.397888001821382 29.74743407957492 22.693936040740002 118-119 19.210870596940342 27.957100850499994 30.533878961320287 22.298149591239373 120-121 18.74165067949658 28.171318849459446 30.74064959806078 22.34638087298319 122-123 19.026083491950292 28.44006287760463 30.217326161670442 22.31652746877464 124-125 19.12193562211642 28.26484729169596 29.966116903379604 22.64710018280801 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 184.0 1 113.0 2 132.0 3 172.5 4 75.5 5 40.0 6 110.5 7 280.5 8 489.5 9 570.0 10 456.5 11 377.5 12 425.0 13 548.0 14 701.0 15 845.5 16 1033.0 17 1238.5 18 1464.5 19 1719.0 20 2024.5 21 2405.5 22 2844.0 23 3306.5 24 3853.0 25 4573.0 26 5584.0 27 6857.0 28 8268.0 29 9707.0 30 11344.0 31 13563.0 32 16384.0 33 19856.0 34 23801.0 35 27145.0 36 29463.5 37 30343.0 38 29192.5 39 27138.5 40 25318.5 41 23960.5 42 22660.0 43 21477.5 44 20550.5 45 19974.0 46 19495.0 47 18980.5 48 19507.5 49 18865.0 50 17387.0 51 15641.0 52 13093.0 53 11130.0 54 9426.5 55 8003.5 56 6518.0 57 5036.5 58 3767.5 59 2709.0 60 1822.0 61 1168.0 62 720.0 63 450.0 64 298.0 65 188.0 66 139.5 67 102.0 68 85.5 69 86.5 70 65.0 71 46.5 72 44.0 73 35.0 74 30.0 75 18.0 76 7.5 77 2.5 78 0.5 79 1.0 80 1.0 81 1.0 82 0.5 83 0.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 1.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018749319918641344 2 0.0 3 0.0 4 0.0 5 0.15484929397092181 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.004687329979660336 22-23 1.674046421307263E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 6.696185685229052E-4 92-93 0.0 94-95 4.185116053268157E-4 96-97 0.0 98-99 9.207255317189945E-4 100-101 0.0 102-103 1.674046421307263E-4 104-105 0.0022599626687648047 106-107 0.007616911216948046 108-109 0.0 110-111 0.0042688183743335205 112-113 0.008453934427601678 114-115 0.004352520695398883 116-117 0.0010044278527843577 118-119 8.370232106536314E-5 120-121 1.674046421307263E-4 122-123 6.696185685229052E-4 124-125 0.001171832494915084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 597355.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.59784603928325 #Duplication Level Percentage of deduplicated Percentage of total 1 84.98054001866855 64.24345780666454 2 10.731596229468867 16.2257111902228 3 2.501774977955974 5.6738639882534105 4 0.820808150168152 2.482053126568034 5 0.32554361852945324 1.2305198176330383 6 0.18127463189150528 0.8222383027537059 7 0.10503250434404898 0.5558161764765236 8 0.06536590458352733 0.3953217272739183 9 0.04620280331606202 0.31435491705038476 >10 0.20418861072785663 2.867514166268751 >50 0.020609141167087244 1.0743535416165053 >100 0.014847382012944037 2.0798462216325997 >500 0.0015512190161852005 0.7664354783448654 >1k 6.648081497936573E-4 1.2685135392409443 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3526 0.5902687681529408 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2575 0.4310669534866202 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1471 0.24625222857429835 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 965 0.16154547965615088 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 669 0.11199370558545589 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 662 0.1108218730905408 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 615 0.10295385491039667 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 8.370232106536314E-5 0.0 0.0 8.370232106536314E-5 0.0 12-13 1.674046421307263E-4 0.0 0.0 1.674046421307263E-4 0.0 14-15 1.674046421307263E-4 0.0 0.0 1.674046421307263E-4 0.0 16-17 1.674046421307263E-4 0.0 0.0 1.674046421307263E-4 0.0 18-19 1.674046421307263E-4 0.0 0.0 3.348092842614526E-4 0.0 20-21 1.674046421307263E-4 0.0 0.0 5.022139263921788E-4 0.0 22-23 3.348092842614526E-4 0.0 0.0 8.370232106536315E-4 0.0 24-25 5.022139263921788E-4 0.0 0.0 8.370232106536315E-4 0.0 26-27 5.022139263921788E-4 0.0 0.0 9.207255317189946E-4 0.0 28-29 5.022139263921788E-4 0.0 0.0 0.0020925580266340786 0.0 30-31 6.696185685229052E-4 0.0 0.0 0.00585916247457542 0.0 32-33 7.533208895882683E-4 0.0 0.0 0.013141264407262014 0.0 34-35 8.370232106536315E-4 0.0 0.0 0.022515924366582687 0.0 36-37 8.370232106536315E-4 0.0 0.0 0.039591197863916766 0.0 38-39 8.370232106536315E-4 0.0 0.0 0.07148178218982013 0.0 40-41 8.370232106536315E-4 0.0 0.0 0.11433737057528606 0.0 42-43 8.370232106536315E-4 0.0 0.0 0.1540122707602682 0.0 44-45 8.370232106536315E-4 0.0 0.0 0.19762118003532236 0.0 46-47 0.001171832494915084 0.0 0.0 0.24842848892199781 0.0 48-49 0.0013392371370458103 0.0 0.0 0.3140511086372425 0.0 50-51 0.0013392371370458103 0.0 0.0 0.39222907651229166 0.0 52-53 0.0013392371370458103 0.0 0.0 0.4591909333645822 0.0 54-55 0.0013392371370458103 0.0 0.0 0.5323467619757096 0.0 56-57 0.0013392371370458103 0.0 0.0 0.620485306057537 0.0 58-59 0.0013392371370458103 0.0 0.0 0.7183333193829464 0.0 60-61 0.0013392371370458103 0.0 0.0 0.8338425224531476 0.0 62-63 0.0015066417791765366 0.0 0.0 0.9542064601451399 0.0 64-65 0.0015066417791765366 0.0 0.0 1.0691297469678833 0.0 66-67 0.001674046421307263 0.0 0.0 1.2033882699567258 0.0 68-69 0.001841451063437989 0.0 0.0 1.3652685588971383 0.0 70-71 0.001841451063437989 0.0 0.0 1.551673627909702 0.0 72-73 0.001841451063437989 0.0 0.0 1.7456956081392137 0.0 74-75 0.001841451063437989 0.0 0.0 1.9259067053929404 0.0 76-77 0.001841451063437989 0.0 0.0 2.117333913669426 0.0 78-79 0.001841451063437989 0.0 0.0 2.343413882866972 0.0 80-81 0.0020088557055687154 0.0 0.0 2.600631115500833 0.0 82-83 0.0020088557055687154 0.0 0.0 2.8665533895254915 0.0 84-85 0.0020088557055687154 0.0 0.0 3.1244402407278757 0.0 86-87 0.0020088557055687154 0.0 0.0 3.3780582735559257 0.0 88-89 0.0020088557055687154 0.0 0.0 3.6808932711704094 0.0 90-91 0.0020088557055687154 0.0 0.0 4.012856676515639 0.0 92-93 0.0020088557055687154 0.0 0.0 4.370600396749002 0.0 94-95 0.0020088557055687154 0.0 0.0 4.710850331879703 0.0 96-97 0.0020088557055687154 0.0 0.0 5.047249960241397 0.0 98-99 0.0020088557055687154 0.0 0.0 5.422571167898486 0.0 100-101 0.0020088557055687154 0.0 0.0 5.844347163746851 0.0 102-103 0.0020088557055687154 0.0 0.0 6.2992692787371 0.0 104-105 0.0020088557055687154 0.0 0.0 6.747913719647446 0.0 106-107 0.0020088557055687154 0.0 0.0 7.178896970813001 0.0 108-109 0.0020088557055687154 0.0 0.0 7.647378861815838 0.0 110-111 0.0020088557055687154 0.0 0.0 8.171104284721816 0.0 112-113 0.0020088557055687154 0.0 0.0 8.748231788467494 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACACCG 30 4.2383326E-5 79.42446 5 GGTATCA 3270 0.0 48.82463 1 GTATCAA 5575 0.0 48.620396 1 TATCAAC 6900 0.0 39.107914 2 ATCAACG 6945 0.0 38.768745 3 TCAACGC 7010 0.0 38.409264 4 CAACGCA 7165 0.0 37.578358 5 AACGCAG 7315 0.0 36.76157 6 ATGGGCG 120 9.1955553E-7 34.748203 5 TACCTGG 595 0.0 34.039055 2 ACATGCG 70 0.002789401 33.996315 9 ACGCAGA 8315 0.0 32.2689 7 CGCAGAG 8335 0.0 32.191475 8 GTACACG 75 0.0038854904 31.772446 1 GCAGAGT 8830 0.0 30.252102 9 TACATGG 6595 0.0 30.168049 2 AGTACTT 4615 0.0 29.843464 12-13 GTACATG 6775 0.0 29.72072 1 GAGTACT 4695 0.0 28.511288 12-13 ACATGGG 6595 0.0 28.36158 3 >>END_MODULE