FastQCFastQC Report
Fri 27 May 2016
SRR939989_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939989_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329560
Sequences flagged as poor quality0
Sequence length125
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA10800.32770967350406605No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA10310.3128413642432334No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT9190.27885665736133025No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA7920.2403204272363151No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC7780.23607233887607718No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6970.21149411336327226No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA5940.1802403204272363No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT5470.16597888093215196No Hit
GGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTC5390.1635514018691589No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA5050.15323461585143827No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA4980.15111057167131933No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA4940.1498968321398228No Hit
TTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTT4190.12713921592426267No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT4030.1222842577982765No Hit
GGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACAT4020.12198082291540237No Hit
TCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTC3700.11227090666343002No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCT52.0593904E-46271.5776110-111
CGCTACC52.0593904E-46271.5776110-111
CGGCTAG52.0593904E-46271.5776110-111
CGTCGCT52.0593904E-46271.5776110-111
CGCCGTG52.0593904E-46271.5776110-111
GTGGTCG52.0593904E-46271.5776110-111
GTGCGAT52.0593904E-46271.5776110-111
CAGCGGC53.429641E-44865.8794108-109
CGACGCC53.429641E-44865.8794108-109
CTGCGCG53.429641E-44865.8794108-109
CGGCTAC53.429641E-44865.8794108-109
AAGCGCG156.232167E-73243.9194108-109
CCGTTCG156.232167E-73243.9194108-109
CTCCGCC400.03135.7888110-111
GGCGTAC108.2367053E-43135.7888110-111
GTTGTCG108.2367053E-43135.7888110-111
CGGCTGT108.2367053E-43135.7888110-111
TGTCGTT108.2367053E-43135.7888110-111
CCGCTCT257.221388E-102919.5273108-109
CCGCTCC500.02919.5273108-109
Produced by FastQC (version 0.11.5)