FastQCFastQC Report
Fri 27 May 2016
SRR939989_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939989_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329560
Sequences flagged as poor quality0
Sequence length125
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA11500.3489501153052555No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA9760.296152445685156No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT9030.27400169923534407No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA8050.24426508071367886No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC6940.21058380871464985No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6730.204211676174293No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA5880.1784197111299915No Hit
GGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTC5730.17386818788687947No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT5350.16233766233766234No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA5290.16051705304041752No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA4950.15020026702269693No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA4760.14443500424808836No Hit
TTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTT4200.1274426508071368No Hit
TCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTC4130.12531860662701785No Hit
GGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACAT3960.12016021361815755No Hit
GTCAAGTGGATGTTTCTCATTTTCCATGATTTTCAGTTTTCTTGCCATAT3470.10529190435732493No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT3390.10286442529433185No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG152.6863563E-4119.151311
GTACACG152.6863563E-4119.151311
GAACGAC251.7130358E-595.321053
CATTCGA208.4334874E-489.363492
AACGACC358.876759E-785.1080864
CTGGGGG509.240466E-1083.405925
CTGTACG651.8189894E-1282.489371
AGCCCAT651.8189894E-1282.489372
ACCTGGG3550.082.231193
TGAACGA304.232929E-579.434212
CTGAACG304.232929E-579.434211
CCTGGGG2100.079.434214
TGTACGA701.8189894E-1276.5972752
TTGGACG658.913048E-1173.323894
CTGGGGA1300.073.323895
CCATGGG900.072.81475
TACGACC250.002045204671.490794
TACCTGG4350.069.847332
GTACCTG4650.065.341051
GTACGAC657.374183E-964.15843
Produced by FastQC (version 0.11.5)