FastQCFastQC Report
Fri 27 May 2016
SRR939988_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939988_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207622
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7040.3390777470595602No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA4970.2393773299553997No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC4450.2143318145475913No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA4090.1969926115729547No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT4000.19265781082929556No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA3810.18350656481490402No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA3600.173392029746366No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA3400.1637591392049012No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3270.1574977603529491No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT2710.13052566683684774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2690.12956237778270124No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA2420.11655797555172381No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2170.10451686237489283No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA2160.1040352178478196No Hit
GGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTC2160.1040352178478196No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA2090.10066370615830693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGT51.9946904E-46350.714110-111
CGTCGCT51.9946904E-46350.714110-111
CGCCGTT51.9946904E-46350.714110-111
CGCCGAT51.9946904E-46350.714110-111
CAGCGCG53.310569E-44939.4443108-109
CTCCGCG53.310569E-44939.4443108-109
CAACGCG53.310569E-44939.4443108-109
CAGCTCG53.310569E-44939.4443108-109
AAGCTCG53.310569E-44939.4443108-109
AATCGGT53.310569E-44939.4443108-109
CGGCGCC155.86273E-73292.963108-109
CCGCGCC155.86273E-73292.963108-109
CGTATTG107.977954E-43175.357110-111
CGGGGCG107.977954E-43175.357110-111
GTTCGTC107.977954E-43175.357110-111
TATCGTG107.977954E-43175.357110-111
CTGTTCG256.5847416E-102963.6665108-109
CTCCGCC359.0221874E-102721.7349110-111
GGCGTCC100.00132405372469.7222108-109
CTGCGCG100.00132405372469.7222108-109
Produced by FastQC (version 0.11.5)