##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939988_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 207622 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.705069790291972 34.0 31.0 34.0 28.0 34.0 2 31.753624375066227 34.0 31.0 34.0 28.0 34.0 3 31.83777730683646 34.0 31.0 34.0 28.0 34.0 4 34.88327826530907 37.0 35.0 37.0 32.0 37.0 5 34.95289034880697 37.0 35.0 37.0 32.0 37.0 6 34.92968471549258 37.0 37.0 37.0 32.0 37.0 7 34.823727735981734 37.0 37.0 37.0 32.0 37.0 8 34.842198803595 37.0 37.0 37.0 32.0 37.0 9 36.43420735760179 39.0 38.0 39.0 32.0 39.0 10-11 36.388010422787566 39.0 38.0 39.0 32.0 39.0 12-13 36.35135486605466 39.0 38.0 39.0 32.0 39.0 14-15 37.91562791996995 41.0 39.0 41.0 32.0 41.0 16-17 37.9705016809394 41.0 39.0 41.0 32.0 41.0 18-19 37.99910173295701 41.0 39.0 41.0 32.0 41.0 20-21 38.08291992178093 41.0 39.0 41.0 32.0 41.0 22-23 38.118515860554275 41.0 39.0 41.0 32.0 41.0 24-25 38.18831096897246 41.0 39.0 41.0 32.5 41.0 26-27 38.198309909354506 41.0 39.0 41.0 32.5 41.0 28-29 38.16815173729181 41.0 39.0 41.0 33.0 41.0 30-31 38.063514463785154 41.0 39.0 41.0 32.0 41.0 32-33 37.96521804047741 41.0 38.0 41.0 32.0 41.0 34-35 37.91618662762135 40.0 38.0 41.0 32.0 41.0 36-37 38.05075810848561 41.0 39.0 41.0 32.0 41.0 38-39 37.9757925460693 41.0 38.5 41.0 32.0 41.0 40-41 37.85076966795426 41.0 38.0 41.0 31.5 41.0 42-43 37.80543487684349 41.0 38.0 41.0 31.0 41.0 44-45 37.77197984799298 41.0 38.0 41.0 31.0 41.0 46-47 37.6390748571924 41.0 38.0 41.0 31.0 41.0 48-49 37.549780370095654 40.0 38.0 41.0 30.0 41.0 50-51 37.448478484938974 40.0 38.0 41.0 30.0 41.0 52-53 37.37326246736858 40.0 38.0 41.0 30.0 41.0 54-55 37.22710261918294 40.0 38.0 41.0 30.0 41.0 56-57 36.97346620300353 40.0 37.0 41.0 30.0 41.0 58-59 36.80300257198178 40.0 37.0 41.0 30.0 41.0 60-61 36.56868973422855 40.0 36.0 41.0 30.0 41.0 62-63 36.2976177861691 40.0 36.0 41.0 29.5 41.0 64-65 35.95019073123272 39.0 35.0 41.0 29.0 41.0 66-67 35.56044879637032 39.0 35.0 41.0 28.5 41.0 68-69 35.04509396884723 38.5 34.5 40.5 28.0 41.0 70-71 34.550644922021746 37.5 34.0 40.0 26.5 41.0 72-73 34.1191444066621 37.0 34.0 39.5 26.0 41.0 74-75 33.63235109959446 36.5 34.0 39.0 26.0 41.0 76-77 33.067003978383795 36.0 33.0 39.0 23.5 40.5 78-79 32.53105403088305 35.5 33.0 38.5 21.0 39.5 80-81 32.04084827234108 35.0 32.0 37.0 19.0 39.0 82-83 31.803416786275058 35.0 33.0 37.0 13.5 39.0 84-85 31.25996040881987 35.0 33.0 36.5 2.0 39.0 86-87 30.51726936451821 35.0 33.0 36.0 2.0 37.0 88-89 15.288529153943223 18.0 16.0 18.5 2.0 19.5 90-91 2.7455712785735615 2.0 2.0 2.0 2.0 2.0 92-93 2.3064077987881824 2.0 2.0 2.0 2.0 2.0 94-95 2.1033247921703864 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1752316504431235 1101 2 0.16365989020192373 1101 3 0.38574422805507425 1101 4 0.4564070373186695 1101 5 0.2536154608919148 1101 6 0.37019312395754156 1101 7 0.1849876451345267 1101 8 0.3545816059437641 1101 9 0.5198968013778753 1101 10-11 0.28522547997739167 1101 12-13 0.23421579159523986 1101 14-15 0.3952447687124945 1101 16-17 0.395553781766111 1101 18-19 0.46813174673225433 1101 20-21 0.5539894925295812 1101 22-23 0.5865659922678361 1101 24-25 0.3748456978680039 1101 26-27 0.5138903275039581 1101 28-29 0.5190224245703234 1101 30-31 0.5361667740717735 1101 32-33 0.6105783625628689 1101 34-35 0.7010021658909338 1101 36-37 0.6596962489006515 1101 38-39 0.7706381480481213 1101 40-41 0.9200325084260612 1101 42-43 0.7458186548050136 1101 44-45 0.5995228612706853 1101 46-47 0.42779943046416946 1101 48-49 0.6466025047428516 1101 50-51 0.5094240669594825 1101 52-53 0.7531838726884033 1101 54-55 0.5446170249069695 1101 56-57 0.6450231672034974 1101 58-59 0.4726139767084945 1101 60-61 0.8154924012565559 1101 62-63 0.9193174295029181 1101 64-65 0.7714783867932979 1101 66-67 1.01199380342765 1101 68-69 1.2419566541314708 1101 70-71 1.4668936574715943 1101 72-73 1.154478591824784 1101 74-75 1.1653622543669826 1101 76-77 1.1834329173605838 1101 78-79 1.225453321674287 1101 80-81 1.2455027935716174 1101 82-83 1.425720647328152 1101 84-85 1.5214354482913492 1101 86-87 1.5045877692621161 1101 88-89 1.413249014239522 1101 90-91 0.11475168524863166 1101 92-93 -0.09486775912168666 1101 94-95 0.04100977201999001 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.3796891777770206 1102 2 0.38510648397820546 1102 3 0.37984491290428934 1102 4 0.44586997135687767 1102 5 0.33733527345950165 1102 6 0.256496139697056 1102 7 0.18469928757114928 1102 8 0.24864624609755737 1102 9 0.34018996490064524 1102 10-11 0.42948836595600426 1102 12-13 0.298399379243925 1102 14-15 0.6434545488398555 1102 16-17 0.5587040881460155 1102 18-19 0.5094930347293598 1102 20-21 0.7435845904510003 1102 22-23 0.6152575698240099 1102 24-25 0.7082110709306093 1102 26-27 0.6665035679220708 1102 28-29 0.8011803003362772 1102 30-31 0.6433817206054755 1102 32-33 0.6007501756110472 1102 34-35 0.6908856213757346 1102 36-37 0.463889448468116 1102 38-39 0.49677655967854406 1102 40-41 0.6700204935275949 1102 42-43 0.2552443426579387 1102 44-45 0.2192453171159272 1102 46-47 0.17280123269894432 1102 48-49 0.13168600828725374 1102 50-51 0.11416394440751532 1102 52-53 0.049297053032027804 1102 54-55 0.037816592370624846 1102 56-57 0.1347444215588922 1102 58-59 -0.041209163985961084 1102 60-61 -0.14247948388102571 1102 62-63 -0.32298702802440715 1102 64-65 -0.053927116030195066 1102 66-67 -0.2306290489092433 1102 68-69 -0.4117739718447382 1102 70-71 -0.3012848839197275 1102 72-73 -0.43211878892734745 1102 74-75 -0.6591661608679118 1102 76-77 -0.5732233363940722 1102 78-79 -0.6968283075459496 1102 80-81 -0.775234921194695 1102 82-83 -0.6600657277769848 1102 84-85 -0.6079770231732695 1102 86-87 -0.38640105688230264 1102 88-89 0.49448775027220115 1102 90-91 0.31450537982991245 1102 92-93 -0.07987917327523686 1102 94-95 0.044614241562221935 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.07981016991253043 1103 2 0.13156746401450548 1103 3 0.25758235997022894 1103 4 0.0764204855734576 1103 5 0.23775874593378887 1103 6 0.35843201386524015 1103 7 0.3641306245342477 1103 8 0.5703038383540573 1103 9 0.6394640120875721 1103 10-11 0.4376553351030097 1103 12-13 0.4052959309680588 1103 14-15 0.39938316347990366 1103 16-17 0.7088553283154084 1103 18-19 0.5418281829447267 1103 20-21 0.43239281791621664 1103 22-23 0.28464655953968077 1103 24-25 0.41420018163841377 1103 26-27 0.42439830476417484 1103 28-29 0.28375743282266086 1103 30-31 0.19501511443488084 1103 32-33 0.4201694133605969 1103 34-35 0.247630932930484 1103 36-37 0.3071441369133723 1103 38-39 0.4622635530239734 1103 40-41 0.39781843666128935 1103 42-43 0.1324070770802095 1103 44-45 0.1955006105218544 1103 46-47 -0.043078985086900445 1103 48-49 0.05906047894665534 1103 50-51 0.08340169395380315 1103 52-53 0.11605446380033158 1103 54-55 -0.06538969922042526 1103 56-57 0.2619374040150362 1103 58-59 0.08725423589304881 1103 60-61 0.011543504624725642 1103 62-63 0.0825181141413438 1103 64-65 0.13491135946888733 1103 66-67 0.39529351612403474 1103 68-69 0.24799614309778661 1103 70-71 0.1885820247312111 1103 72-73 0.2325212594695074 1103 74-75 0.32761544832749223 1103 76-77 0.33999505441052946 1103 78-79 0.2803344268763084 1103 80-81 0.3443658047581124 1103 82-83 0.8768973696216911 1103 84-85 0.5729667154837195 1103 86-87 0.565141592845464 1103 88-89 1.6575549009074102 1103 90-91 -0.40554906663106705 1103 92-93 -0.3126377939648921 1103 94-95 -0.11055817223088127 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.06533687034999858 1104 2 0.04258601759269354 1104 3 -0.14975072093347208 1104 4 -0.17608487855629562 1104 5 0.04557144314443917 1104 6 -0.05456811234957826 1104 7 0.1571130806745984 1104 8 -0.2959084549691795 1104 9 0.11106567812316825 1104 10-11 0.19706464975655535 1104 12-13 0.040547729529215815 1104 14-15 0.2893364639973868 1104 16-17 0.11878843446206844 1104 18-19 0.050921960554219936 1104 20-21 -0.13287286427486578 1104 22-23 -0.15969901663096664 1104 24-25 0.09956557353542195 1104 26-27 -0.2802590220059855 1104 28-29 -0.30643567584829867 1104 30-31 -0.2885706485336925 1104 32-33 -0.3208945775519325 1104 34-35 -0.07129497897157222 1104 36-37 -0.09443354830688122 1104 38-39 -0.23742090993196996 1104 40-41 -0.31374284063267055 1104 42-43 -0.39698835394339227 1104 44-45 -0.15464453651840415 1104 46-47 -0.4054795909180271 1104 48-49 -0.5567163732557887 1104 50-51 -0.4370640387232214 1104 52-53 -0.29760197946611555 1104 54-55 -0.17690680132820802 1104 56-57 -0.06201816836916407 1104 58-59 -0.38340712975128355 1104 60-61 -0.34301285029133766 1104 62-63 -0.388938404853981 1104 64-65 -0.5195187806295536 1104 66-67 -0.11181237810794897 1104 68-69 -0.2517640487487398 1104 70-71 -0.019382130260588326 1104 72-73 -0.03494687128904417 1104 74-75 0.011908014274737866 1104 76-77 -0.24897377052952407 1104 78-79 -0.34150704731275283 1104 80-81 -0.22017414223165943 1104 82-83 -0.11516371835004335 1104 84-85 0.010963686328096856 1104 86-87 -0.18008748704851385 1104 88-89 0.5564946964394064 1104 90-91 -0.4744055603834334 1104 92-93 -0.3126377939648921 1104 94-95 -0.11055817223088127 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 0.10992617654147807 1105 2 0.12608143624148838 1105 3 0.0250319285170022 1105 4 -0.003001195665476075 1105 5 0.3030996225553082 1105 6 0.2904509863779552 1105 7 0.013790605104567533 1105 8 -0.055217620014992974 1105 9 0.08743246742788102 1105 10-11 -0.06632203503242806 1105 12-13 0.17044251077262373 1105 14-15 0.1304420811323439 1105 16-17 -0.07972116176973998 1105 18-19 0.21883258319617482 1105 20-21 0.036238751969591476 1105 22-23 0.1060134947931175 1105 24-25 0.2945190570027236 1105 26-27 0.0710638285083931 1105 28-29 0.1981925434004097 1105 30-31 -0.15021032701891812 1105 32-33 -0.09246559435413104 1105 34-35 0.05151686926969745 1105 36-37 0.03029403119304419 1105 38-39 0.009620544852083412 1105 40-41 0.1457983212261169 1105 42-43 0.031561314465200496 1105 44-45 0.04077176887376055 1105 46-47 0.30566094290898604 1105 48-49 0.1970740797238406 1105 50-51 0.0669607544715376 1105 52-53 0.05389662620960678 1105 54-55 -0.14098830485503555 1105 56-57 -0.13903408006977003 1105 58-59 -0.11280862484185405 1105 60-61 -0.3058289166564876 1105 62-63 -0.1543697080199209 1105 64-65 -0.4102283628009502 1105 66-67 -0.3052836597145898 1105 68-69 -0.3434262238946104 1105 70-71 -0.5178354848407665 1105 72-73 -0.8631813521082989 1105 74-75 -0.5770533898918302 1105 76-77 -0.5159224265883822 1105 78-79 -0.47295433248136476 1105 80-81 -0.4393818379643264 1105 82-83 -0.3425323120744075 1105 84-85 -0.23219357939027319 1105 86-87 -0.23833860602304213 1105 88-89 0.3960771021760543 1105 90-91 -0.7423911718942251 1105 92-93 -0.3126377939648921 1105 94-95 -0.11055817223088127 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.16509557270488529 1106 2 -0.042672483680821216 1106 3 0.0649594984686459 1106 4 -0.06321120234198929 1106 5 -0.0458088687551097 1106 6 -0.15571204192641375 1106 7 -0.24040244727571292 1106 8 0.101741127228415 1106 9 0.025047536115032187 1106 10-11 0.17577236681272268 1106 12-13 -0.07327549760336893 1106 14-15 -0.22087400117084854 1106 16-17 -0.09618989343462658 1106 18-19 -0.0413743887017759 1106 20-21 0.00729202244928473 1106 22-23 -0.03898316695045878 1106 24-25 -0.06546637606017924 1106 26-27 -0.44001817120836506 1106 28-29 -0.061306313499663645 1106 30-31 -0.18512545437846484 1106 32-33 -0.17074264940501394 1106 34-35 -0.11595738569216252 1106 36-37 -0.2786464264515658 1106 38-39 -0.25492616038999927 1106 40-41 -0.3539032588819211 1106 42-43 -0.39737934549797416 1106 44-45 -0.0974927424257217 1106 46-47 -0.28258535971912835 1106 48-49 -0.38176440936106104 1106 50-51 -0.252884674140347 1106 52-53 -0.09252132349399034 1106 54-55 -0.27476640184655565 1106 56-57 -0.5696034045280598 1106 58-59 -0.2425049446670542 1106 60-61 -0.04725566721792518 1106 62-63 -0.01690435507516952 1106 64-65 -0.3655463064349931 1106 66-67 -0.1190848210231863 1106 68-69 -0.006869281332853916 1106 70-71 0.034686416144523946 1106 72-73 0.28805683754512756 1106 74-75 0.2228535435655914 1106 76-77 0.36556441258858285 1106 78-79 0.42663496081986807 1106 80-81 0.4960330162651445 1106 82-83 0.29142281366658196 1106 84-85 0.06332304108032005 1106 86-87 0.38498467882127585 1106 88-89 0.9940701019535041 1106 90-91 -0.304502973136461 1106 92-93 -0.3126377939648921 1106 94-95 -0.11055817223088127 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.6586715484019479 1107 2 -0.5606771662527521 1107 3 -0.45866620536835256 1107 4 -0.5123317688944908 1107 5 -0.40113287564351907 1107 6 -0.545748999415629 1107 7 -0.8055037417553095 1107 8 -0.6335000639250055 1107 9 -0.7925740269352133 1107 10-11 -0.7382095607815558 1107 12-13 -0.6579439444213406 1107 14-15 -0.5768752395369816 1107 16-17 -0.5232810874904601 1107 18-19 -0.818657142518461 1107 20-21 -0.4923161507671736 1107 22-23 -0.7037777723180056 1107 24-25 -0.6942274294453696 1107 26-27 -0.6415961205514478 1107 28-29 -0.782040238926804 1107 30-31 -0.7975410423121332 1107 32-33 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-0.8688535536781359 2216 68-69 -0.9391492469731375 2216 70-71 -1.2699752727678586 2216 72-73 -1.1833568666969754 2216 74-75 -1.2402923530909504 2216 76-77 -1.263639697362521 2216 78-79 -0.9169126802532119 2216 80-81 -0.939550768590145 2216 82-83 -1.358983556096863 2216 84-85 -1.3510254457704818 2216 86-87 -1.2599703892374023 2216 88-89 -1.654329197636823 2216 90-91 -0.7423911718942251 2216 92-93 -0.3126377939648921 2216 94-95 -0.11055817223088127 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2833.0 3 380.0 4 398.0 5 489.0 6 382.0 7 269.0 8 332.0 9 393.0 10 508.0 11 721.0 12 808.0 13 1099.0 14 1258.0 15 1385.0 16 1468.0 17 1631.0 18 2155.0 19 2273.0 20 2356.0 21 3266.0 22 3517.0 23 4215.0 24 6802.0 25 12162.0 26 20489.0 27 50017.0 28 82806.0 29 3081.0 30 129.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.80251611100943 21.301692498868135 14.88859562088796 23.007195769234475 2 14.66676941898381 20.976403893670618 45.63647835699045 18.720348330355122 3 18.553944008095215 29.632824169999516 30.44668240736279 21.366549414542476 4 11.083803190207702 19.78651631246687 38.82704447978411 30.302636017541325 5 9.56571186244651 38.02334322834342 32.12585296272023 20.285091946489842 6 23.474390960495516 35.52561867239503 25.592181946036547 15.407808421072911 7 25.994355126142704 33.39771315178546 23.59287551415553 17.01505620791631 8 25.212164414175763 38.450645885310806 21.080136016414443 15.257053684098986 9 25.24154473032723 22.95614144936471 24.232499446108793 27.569814374199265 10-11 22.685380297224985 26.385400285617266 32.0144205413242 18.91479887583355 12-13 26.85881349972787 25.758480678550615 29.444800331376225 17.937905490345297 14-15 19.9506796004277 33.5499128223406 25.247565286915645 21.251842290316056 16-17 18.816783506445656 32.3949224667121 31.280148154926895 17.508145871915346 18-19 20.88112962619546 28.830226122726188 32.70603082243743 17.58261342864093 20-21 21.220944234122282 30.014738606479206 28.44888208151509 20.31543507788342 22-23 19.179899913785214 33.79908583428459 29.13192788783408 17.88908636409612 24-25 20.448940863684967 30.998400940170118 31.64789858492838 16.904759611216537 26-27 17.552812322393578 33.048761691920895 30.628016298850795 18.77040968683473 28-29 17.522595312119947 34.383887910875856 27.318111650824463 20.775405126179734 30-31 18.86866517035767 30.532891504753835 30.4281338201154 20.170309504773098 32-33 17.878137650846863 31.71667401183415 32.67178660963927 17.73340172767972 34-35 18.437352496363584 32.42430956257044 30.99454778395353 18.143790157112445 36-37 17.447855833812973 32.45015569196832 29.909474693131493 20.192513781087218 38-39 19.733939563244743 33.575439982275476 27.819306239223202 18.87131421525657 40-41 22.28280240051632 28.91721493868665 31.64910269624606 17.150879964550963 42-43 18.124042731502442 33.273689686064095 30.71109997977093 17.89116760266253 44-45 17.5641309687798 33.56773366984231 29.16237200296693 19.70576335841096 46-47 21.152864340002505 30.575276223136278 29.144310333201684 19.127549103659536 48-49 18.59609915158112 31.6207618189831 29.530419537475648 20.252719491960132 50-51 16.48837791756172 33.658523663195616 30.446436312144186 19.406662107098477 52-53 16.618662762135035 31.570594638333127 31.480045467243357 20.330697132288485 54-55 17.96997859053986 30.311123527046004 30.608784862620624 21.110113019793516 56-57 19.46831260656385 31.620926491412277 26.83024920287831 22.080511699145564 58-59 19.138145283255145 33.359181589619595 26.249385903227985 21.253287223897274 60-61 18.100201327412314 29.136363198504977 33.390729306142894 19.372706167939814 62-63 19.119601968962826 28.9550240340619 31.840074751230603 20.08529924574467 64-65 20.558755815857666 31.519540318463363 27.217491402645194 20.704212463033784 66-67 19.98270896147807 34.02192445887237 26.441802891793742 19.553563687855817 68-69 21.32279808593082 29.013753458834753 31.204288593852723 18.459159861381703 70-71 22.78082284151005 29.00053944187032 29.341543767038175 18.87709394958145 72-73 19.977651693943802 32.617689840190344 27.959705618865055 19.4449528470008 74-75 19.821358044908536 32.543516583021066 30.342641916559902 17.292483455510496 76-77 20.382714741212396 30.179364421882077 31.296538902428452 18.14138193447708 78-79 19.352329484975026 29.743378559972257 30.591799480784697 20.31249247426802 80-81 19.77174865861999 31.418394967777978 30.071235225554133 18.738621148047898 82-83 19.173925503502783 30.688443578548362 31.870889435291794 18.26674148265706 84-85 18.81351687200778 31.566982304380076 31.18094421593087 18.438556607681267 86-87 16.81878826118997 34.78318458329922 27.412231057851162 20.985796097659655 88-89 28.068218755684647 20.59236639400238 35.269120545845475 16.0702943044675 90-91 30.440755170095997 17.02819815057721 18.477587005749264 34.05345967357753 92-93 22.17337823395374 18.790909640862253 38.23959992740618 20.796112197777823 94-95 16.606733942238204 7.776597504592907 29.81036299205057 45.80630556111832 96-97 28.784025311611888 7.771747499871669 16.467014172449357 46.97721301606709 98-99 57.78092019038422 13.909774436090224 18.67972684003587 9.629578533489687 100-101 31.117707409879173 7.319733447551124 13.681232755271783 47.88132638729792 102-103 23.32293010987655 2.276197870338473 38.67274064095511 35.72813137882987 104-105 40.24633980740358 4.375974218473573 44.83460997790974 10.5430759962131 106-107 6.74154358282112 2.678793756470912 7.615690077716472 82.9639725829915 108-109 19.505136339706787 12.367732706370054 24.71369075017847 43.41344020374469 110-111 41.37505292774522 3.087814549095183 27.548940851513436 27.988191671646163 112-113 27.603085286713007 2.9177825254014587 40.811342139840924 28.667790048044616 114-115 29.803399959914728 4.185266840369514 25.82038153891005 40.190951660805716 116-117 20.84811986952002 4.799799261569344 22.013836613255904 52.33824425565473 118-119 21.820336208954206 4.674020527159273 17.846182698537184 55.65946056534934 120-121 26.455084559631015 4.584278985772507 32.23271591380531 36.727920540791175 122-123 35.16678107062206 1.8433959073228479 35.68408591239641 27.30573710965868 124-125 40.66250577011848 2.571068625942453 20.828685182335743 35.93774042160332 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10.0 1 46.0 2 120.5 3 196.5 4 243.5 5 244.0 6 210.0 7 158.0 8 116.0 9 93.5 10 88.5 11 74.5 12 53.0 13 53.5 14 59.5 15 61.0 16 67.0 17 85.5 18 116.0 19 131.5 20 163.5 21 228.0 22 318.0 23 457.0 24 591.5 25 753.0 26 1043.0 27 1409.5 28 1922.5 29 2782.0 30 4055.0 31 5961.5 32 8609.0 33 11537.5 34 14095.0 35 15735.0 36 16091.5 37 15273.5 38 13807.0 39 12196.0 40 10529.0 41 8749.5 42 7152.0 43 6058.5 44 5248.0 45 4521.0 46 4033.5 47 3730.5 48 3528.5 49 3410.5 50 3245.0 51 2991.5 52 2694.5 53 2380.5 54 2083.5 55 1736.5 56 1400.0 57 1140.0 58 905.5 59 708.5 60 547.5 61 407.0 62 296.5 63 208.0 64 142.5 65 106.5 66 71.0 67 50.0 68 45.0 69 41.5 70 34.0 71 27.0 72 24.0 73 18.5 74 13.5 75 11.0 76 11.5 77 12.0 78 8.5 79 6.5 80 7.5 81 6.5 82 5.0 83 3.5 84 2.5 85 2.5 86 1.0 87 2.0 88 2.5 89 1.0 90 1.5 91 1.5 92 0.5 93 1.0 94 1.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0014449335812197165 3 0.04431129649073798 4 0.05394418703220275 5 0.052980897978056274 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.224667906098582E-4 12-13 0.0014449335812197165 14-15 0.0 16-17 7.224667906098582E-4 18-19 0.006502201115488725 20-21 0.0019265781082929555 22-23 4.816445270732389E-4 24-25 0.0 26-27 0.0 28-29 7.224667906098582E-4 30-31 0.0 32-33 0.0012041113176830972 34-35 0.0 36-37 2.4082226353661944E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.4082226353661944E-4 50-51 0.0 52-53 0.0 54-55 0.001685755844756336 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.224667906098582E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 4.816445270732389E-4 80-81 0.0 82-83 7.224667906098582E-4 84-85 0.0 86-87 0.0014449335812197165 88-89 19.244347901474796 90-91 26.194237604878097 92-93 40.28691564477753 94-95 44.68264442111145 96-97 48.39443796900136 98-99 93.01759929101927 100-101 51.64626099353633 102-103 53.16223714249935 104-105 74.81697507971217 106-107 81.62453882536532 108-109 12.627997033069713 110-111 39.14349153750566 112-113 51.98052229532516 114-115 86.78295171031971 116-117 66.40890657059464 118-119 71.74962190904624 120-121 80.2636522141199 122-123 75.79230524703547 124-125 49.91667549681633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 207622.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.00735040938929 #Duplication Level Percentage of deduplicated Percentage of total 1 85.71800407322078 56.58018331254543 2 9.071949302664525 11.976306730343842 3 2.111436257425567 4.181109387329366 4 0.9444239700631442 2.4935569570793814 5 0.47287035725755044 1.5606459684856115 6 0.3320971347567658 1.3152511166306406 7 0.21505036432519226 0.9936433327575855 8 0.15476103316190748 0.8172292596509714 9 0.13402254857468596 0.7961825993875824 >10 0.7054741953323267 8.586787957842015 >50 0.08966112290699892 4.085573364553328 >100 0.0492643532456038 6.155675246019937 >500 9.85287064912076E-4 0.45785476737433023 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 704 0.3390777470595602 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 497 0.2393773299553997 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 445 0.2143318145475913 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 409 0.1969926115729547 No Hit ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT 400 0.19265781082929556 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 381 0.18350656481490402 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA 360 0.173392029746366 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 340 0.1637591392049012 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 327 0.1574977603529491 No Hit TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT 271 0.13052566683684774 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 269 0.12956237778270124 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 242 0.11655797555172381 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 217 0.10451686237489283 No Hit ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA 216 0.1040352178478196 No Hit GGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTC 216 0.1040352178478196 No Hit GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA 209 0.10066370615830693 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.4082226353661944E-4 0.0 24-25 0.0 0.0 0.0 4.816445270732389E-4 0.0 26-27 0.0 0.0 0.0 0.0014449335812197167 0.0 28-29 0.0 0.0 0.0 0.002167400371829575 0.0 30-31 0.0 0.0 0.0 0.00433480074365915 0.0 32-33 0.0 0.0 0.0 0.00818795696024506 0.0 34-35 0.0 0.0 0.0 0.01276357996744083 0.0 36-37 0.0 0.0 0.0 0.02143318145475913 0.0 38-39 0.0 0.0 0.0 0.040698962537688683 0.0 40-41 0.0 0.0 0.0 0.06526283341842387 0.0 42-43 0.0 0.0 0.0 0.08597354808257314 0.0 44-45 0.0 0.0 0.0 0.10981495217269846 0.0 46-47 0.0 0.0 0.0 0.13606457889819 0.0 48-49 0.0 0.0 0.0 0.17266956295575614 0.0 50-51 0.0 0.0 0.0 0.2184257930277138 0.0 52-53 0.0 0.0 0.0 0.2718883355328433 0.0 54-55 0.0 0.0 0.0 0.3455799481750489 0.0 56-57 0.0 0.0 0.0 0.42794116230457274 0.0 58-59 0.0 0.0 0.0 0.5069308647445839 0.0 60-61 0.0 0.0 0.0 0.5941085241448401 0.0 62-63 0.0 0.0 0.0 0.6880292069241217 0.0 64-65 0.0 0.0 0.0 0.7925460692990145 0.0 66-67 0.0 0.0 0.0 0.909344867114275 0.0 68-69 0.0 0.0 0.0 1.0634711157777113 0.0 70-71 0.0 0.0 0.0 1.2151891418057816 0.0 72-73 0.0 0.0 0.0 1.3738910134764137 0.0 74-75 0.0 0.0 0.0 1.5480055100133896 0.0 76-77 0.0 0.0 0.0 1.7365693423625626 0.0 78-79 0.0 0.0 0.0 1.784974617333423 0.0 80-81 0.0 0.0 0.0 1.7859379063875698 0.0 82-83 0.0 0.0 0.0 1.7859379063875698 0.0 84-85 0.0 0.0 0.0 1.7859379063875698 0.0 86-87 0.0 0.0 0.0 1.7859379063875698 0.0 88-89 0.0 0.0 0.0 1.7859379063875698 0.0 90-91 0.0 0.0 0.0 1.7859379063875698 0.0 92-93 0.0 0.0 0.0 1.7859379063875698 0.0 94-95 0.0 0.0 0.0 1.7859379063875698 0.0 96-97 0.0 0.0 0.0 1.7859379063875698 0.0 98-99 0.0 0.0 0.0 1.7859379063875698 0.0 100-101 0.0 0.0 0.0 1.7859379063875698 0.0 102-103 0.0 0.0 0.0 1.7859379063875698 0.0 104-105 0.0 0.0 0.0 1.7859379063875698 0.0 106-107 0.0 0.0 0.0 1.7859379063875698 0.0 108-109 0.0 0.0 0.0 1.7859379063875698 0.0 110-111 0.0 0.0 0.0 1.7859379063875698 0.0 112-113 0.0 0.0 0.0 1.7859379063875698 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGGT 5 1.9946904E-4 6350.714 110-111 CGTCGCT 5 1.9946904E-4 6350.714 110-111 CGCCGTT 5 1.9946904E-4 6350.714 110-111 CGCCGAT 5 1.9946904E-4 6350.714 110-111 CAGCGCG 5 3.310569E-4 4939.4443 108-109 CTCCGCG 5 3.310569E-4 4939.4443 108-109 CAACGCG 5 3.310569E-4 4939.4443 108-109 CAGCTCG 5 3.310569E-4 4939.4443 108-109 AAGCTCG 5 3.310569E-4 4939.4443 108-109 AATCGGT 5 3.310569E-4 4939.4443 108-109 CGGCGCC 15 5.86273E-7 3292.963 108-109 CCGCGCC 15 5.86273E-7 3292.963 108-109 CGTATTG 10 7.977954E-4 3175.357 110-111 CGGGGCG 10 7.977954E-4 3175.357 110-111 GTTCGTC 10 7.977954E-4 3175.357 110-111 TATCGTG 10 7.977954E-4 3175.357 110-111 CTGTTCG 25 6.5847416E-10 2963.6665 108-109 CTCCGCC 35 9.0221874E-10 2721.7349 110-111 GGCGTCC 10 0.0013240537 2469.7222 108-109 CTGCGCG 10 0.0013240537 2469.7222 108-109 >>END_MODULE