FastQCFastQC Report
Fri 27 May 2016
SRR939988_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939988_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207622
Sequences flagged as poor quality0
Sequence length125
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.47586479274835997No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7970.3838706880773714No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6770.32607334482858275No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.2557532438758898No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA4800.23118937299515466No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC4470.21529510360173776No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT4270.20566221306027302No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA3590.17291038521929275No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA3460.16664900636734065No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3440.16568571731319418No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.1647224282590477No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA3410.16424078373197445No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA3340.1608692720424618No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA2990.14401171359489842No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2550.1228193544036759No Hit
GGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTC2450.11800290913294353No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC2340.1127048193351379No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA2320.11174153028099142No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT2310.11125988575391818No Hit
TTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTT2180.10499850690196606No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG251.1906195E-7119.214532
ATAACGC303.5297853E-799.345443
TCACGCA251.7070302E-595.371634
CACGCAG251.7070302E-595.371635
ATCACGC251.7070302E-595.371633
ACCTGGG5200.087.118313
CTGGGGG900.086.099385
TAACGCA358.83716E-785.153244
GTACCTG5600.083.024411
TACCTGG5650.082.289672
CCTGGGG3050.082.082134
CGGACCT304.2181164E-579.476365
GACGGAC250.002039836571.528723
CTGGGGA2100.065.284145
CCTGGGA1550.061.530084
ATGACGT401.7528977E-459.607265
TGACGTG401.7697005E-459.492416
TGGGGAG1650.050.4784136
TGTATAG1950.048.9085242
GTAAGAC751.4468715E-647.6858063
Produced by FastQC (version 0.11.5)