##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939988_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 207622 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.84460702623036 34.0 31.0 34.0 31.0 34.0 2 32.93558004450396 34.0 33.0 34.0 31.0 34.0 3 32.964271608981704 34.0 33.0 34.0 31.0 34.0 4 36.359952220862915 37.0 37.0 37.0 35.0 37.0 5 36.27817379661115 37.0 37.0 37.0 35.0 37.0 6 36.42521505428134 37.0 37.0 37.0 35.0 37.0 7 36.43543555114583 37.0 37.0 37.0 35.0 37.0 8 36.46653533825895 37.0 37.0 37.0 35.0 37.0 9 38.311277224956896 39.0 39.0 39.0 37.0 39.0 10-11 38.302024833591815 39.0 39.0 39.0 37.0 39.0 12-13 38.29096627525021 39.0 39.0 39.0 37.0 39.0 14-15 39.819535020373564 41.0 40.0 41.0 38.0 41.0 16-17 39.85655421872441 41.0 40.0 41.0 38.0 41.0 18-19 39.89385758734623 41.0 40.0 41.0 38.0 41.0 20-21 39.85954523123753 41.0 40.0 41.0 37.5 41.0 22-23 39.73070772846808 41.0 40.0 41.0 36.5 41.0 24-25 39.67895502403407 41.0 40.0 41.0 36.0 41.0 26-27 39.56307616726551 41.0 40.0 41.0 36.0 41.0 28-29 39.45328529731917 41.0 40.0 41.0 36.0 41.0 30-31 39.226869021587305 41.0 39.0 41.0 35.0 41.0 32-33 38.917024689098454 41.0 39.0 41.0 35.0 41.0 34-35 38.634386047721335 41.0 39.0 41.0 35.0 41.0 36-37 38.49145562608972 41.0 39.0 41.0 35.0 41.0 38-39 38.482258623845254 41.0 39.0 41.0 35.0 41.0 40-41 38.34019997880765 41.0 39.0 41.0 35.0 41.0 42-43 38.2446272553005 41.0 39.0 41.0 35.0 41.0 44-45 38.07721243413511 41.0 39.0 41.0 35.0 41.0 46-47 37.7874382290894 41.0 39.0 41.0 34.0 41.0 48-49 37.51678771999114 41.0 39.0 41.0 33.0 41.0 50-51 37.3893204959012 41.0 39.0 41.0 33.0 41.0 52-53 37.307508356532544 41.0 39.0 41.0 33.0 41.0 54-55 37.214632842377014 41.0 38.5 41.0 33.0 41.0 56-57 37.054982130988044 41.0 38.0 41.0 32.0 41.0 58-59 36.905460404003435 40.0 38.0 41.0 32.0 41.0 60-61 36.716800242748846 40.0 37.5 41.0 31.0 41.0 62-63 36.5314730616216 40.0 37.0 41.0 31.0 41.0 64-65 36.27431823217192 39.5 37.0 41.0 31.0 41.0 66-67 35.982615040795295 39.0 36.0 41.0 30.5 41.0 68-69 35.608360385700934 39.0 35.5 41.0 29.5 41.0 70-71 35.22143607132192 38.5 35.0 40.5 29.0 41.0 72-73 34.868621822350235 37.0 35.0 40.0 29.0 41.0 74-75 34.5208841066939 37.0 35.0 39.0 29.0 41.0 76-77 34.11517324753639 36.0 35.0 39.0 28.0 41.0 78-79 33.751945843889374 36.0 35.0 39.0 28.0 40.0 80-81 33.49960023504253 35.5 35.0 37.0 28.5 39.0 82-83 33.269374151101516 35.0 35.0 37.0 29.0 39.0 84-85 32.967737041354 35.0 34.5 37.0 28.0 39.0 86-87 32.74860804731676 35.0 34.0 36.0 27.5 37.0 88-89 32.507956767587245 35.0 34.0 36.0 27.0 37.0 90-91 32.27072516399996 35.0 34.0 36.0 26.0 37.0 92-93 32.14189006945314 35.0 34.0 35.5 26.0 36.0 94-95 32.037570681334344 35.0 34.0 35.0 26.0 36.0 96-97 31.863815009970043 35.0 34.0 35.0 26.0 36.0 98-99 31.678454113725905 35.0 34.0 35.0 25.0 36.0 100-101 31.72782990241882 35.0 34.0 35.0 25.0 36.0 102-103 30.884215063914226 34.5 32.0 35.0 24.0 35.0 104-105 31.541072237046173 35.0 34.0 35.0 25.0 35.0 106-107 31.60131151804722 35.0 34.0 35.0 25.0 35.0 108-109 31.61659169066862 35.0 34.0 35.0 25.0 35.0 110-111 31.60119833158336 35.0 34.0 35.0 25.0 35.0 112-113 31.504977796187305 35.0 34.0 35.0 25.0 35.0 114-115 31.392268160406893 35.0 34.0 35.0 24.0 35.0 116-117 31.327638689541573 35.0 34.0 35.0 23.0 35.0 118-119 31.237903497702554 35.0 34.0 35.0 21.5 35.0 120-121 31.12181994201 35.0 34.0 35.0 18.5 35.0 122-123 30.93538016202522 35.0 34.0 35.0 8.5 35.0 124-125 28.812211615339415 33.0 29.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11374533255594343 1101 2 0.23163018651710843 1101 3 0.17501941885657857 1101 4 0.041103186696304306 1101 5 -0.030596184752653244 1101 6 0.03152451974255399 1101 7 0.060128252074378 1101 8 0.15666170593570428 1101 9 0.193345559393876 1101 10-11 0.12432926314092896 1101 12-13 0.14464851093672593 1101 14-15 0.27854134283295195 1101 16-17 0.2581960768190825 1101 18-19 0.26960691764735145 1101 20-21 0.2814270847122273 1101 22-23 0.3338313428254054 1101 24-25 0.31840723380227587 1101 26-27 0.2921313213312189 1101 28-29 0.2840365856421272 1101 30-31 0.4708429793519997 1101 32-33 0.520110594882226 1101 34-35 0.5674271351667457 1101 36-37 0.6257918100600293 1101 38-39 0.6210557307161437 1101 40-41 0.5836549227085328 1101 42-43 0.4077543424867258 1101 44-45 0.8203195838861745 1101 46-47 1.0595285060405573 1101 48-49 0.9290759636368477 1101 50-51 0.8646907349003357 1101 52-53 0.897164198027383 1101 54-55 0.8603050785893913 1101 56-57 0.9482833009285656 1101 58-59 0.9729192867033447 1101 60-61 0.7872971494953376 1101 62-63 0.907015290016254 1101 64-65 0.853290133548164 1101 66-67 0.7656981788583082 1101 68-69 0.9128200641996571 1101 70-71 0.7483261983038005 1101 72-73 0.7670456476750331 1101 74-75 0.7526598543728582 1101 76-77 0.8313639045856718 1101 78-79 0.8291186457995039 1101 80-81 0.6041296245925807 1101 82-83 0.8249075749287869 1101 84-85 0.7675562639619571 1101 86-87 0.7800182559031654 1101 88-89 0.8866780816003796 1101 90-91 0.8750217883140863 1101 92-93 0.8507700335294146 1101 94-95 0.8190167431859336 1101 96-97 0.7030159025429725 1101 98-99 1.010629099902964 1101 100-101 1.1215255257211112 1101 102-103 0.9985966030189211 1101 104-105 0.8542548252751807 1101 106-107 0.8955403901044612 1101 108-109 1.093434789386059 1101 110-111 0.8761674683421887 1101 112-113 0.739193418573592 1101 114-115 1.034591117648752 1101 116-117 1.1425388471028057 1101 118-119 1.0749766766296496 1101 120-121 0.9901425435640796 1101 122-123 1.1191503298385364 1101 124-125 1.2724609155917967 1102 1 -0.00661992035767156 1102 2 0.17120726209081738 1102 3 0.0907469209591838 1102 4 -0.18696361613920942 1102 5 -0.1882917272253195 1102 6 -0.03875062143249863 1102 7 0.09976540214046281 1102 8 0.15358589192633332 1102 9 0.015741330149616317 1102 10-11 0.16996785499482314 1102 12-13 0.14899790418435543 1102 14-15 0.16045291318018684 1102 16-17 0.3156813585684546 1102 18-19 0.1835922594712116 1102 20-21 0.1076075484873158 1102 22-23 0.17271155428762341 1102 24-25 0.22004125999475121 1102 26-27 -0.11186963986066445 1102 28-29 -0.07024672566388546 1102 30-31 0.01675791387080494 1102 32-33 0.08419205589387957 1102 34-35 0.14430446305332367 1102 36-37 0.32086570168564776 1102 38-39 0.019704054637806223 1102 40-41 0.13042291501900394 1102 42-43 0.07795138682170943 1102 44-45 0.09999037566798563 1102 46-47 0.13478382263313193 1102 48-49 0.25348422988699326 1102 50-51 0.28445524289024604 1102 52-53 0.3395767896409865 1102 54-55 0.16942438653123304 1102 56-57 0.3978147198880748 1102 58-59 0.43407511993655135 1102 60-61 0.19710130665021808 1102 62-63 0.3830936271543024 1102 64-65 0.32088595236349704 1102 66-67 0.23521037398025868 1102 68-69 0.14494790271941582 1102 70-71 0.30161227303646854 1102 72-73 0.09426540016812623 1102 74-75 -0.14356146005703607 1102 76-77 -0.2353187819456224 1102 78-79 0.004025530336328131 1102 80-81 0.1839205653591378 1102 82-83 0.03399083817520676 1102 84-85 0.002783946288040795 1102 86-87 -0.20324499052239986 1102 88-89 -0.1676653041980103 1102 90-91 -0.12523653200310747 1102 92-93 0.05895818683951859 1102 94-95 0.03205891547957407 1102 96-97 -0.23610460688873047 1102 98-99 -0.19552252811578086 1102 100-101 0.015746359555066647 1102 102-103 -0.005782827474888563 1102 104-105 0.020510982910646902 1102 106-107 -0.2010834234243113 1102 108-109 0.0012564882926966447 1102 110-111 -0.023832531657809852 1102 112-113 -0.14113218517505288 1102 114-115 -0.39249046351189065 1102 116-117 -0.25031228830510344 1102 118-119 -0.22677749854756613 1102 120-121 -0.19238779405457151 1102 122-123 -0.06966379968206837 1102 124-125 -0.19081554722209404 1103 1 0.15563053336283872 1103 2 0.22583521127412354 1103 3 0.34095865719633167 1103 4 0.2156982108420351 1103 5 0.1044487446440101 1103 6 0.27334859211861584 1103 7 0.18645626723422737 1103 8 0.1575786323981987 1103 9 0.28458585525547875 1103 10-11 0.16588437042131687 1103 12-13 0.21070389329506156 1103 14-15 0.39665980973191495 1103 16-17 0.31686103188968673 1103 18-19 0.18643557465572513 1103 20-21 0.2736087584086633 1103 22-23 0.22207634557618405 1103 24-25 0.22899467802257334 1103 26-27 0.2088805113795118 1103 28-29 0.2243691243058592 1103 30-31 0.18121647406438512 1103 32-33 0.16026586109654062 1103 34-35 0.10241093613257846 1103 36-37 -0.09250392928833406 1103 38-39 -0.28855365861083015 1103 40-41 -0.3305268732447644 1103 42-43 -0.3714436525007372 1103 44-45 -0.1834337017669796 1103 46-47 0.06052489946313955 1103 48-49 0.27275222746715144 1103 50-51 0.0033300160299916115 1103 52-53 0.24441647506143482 1103 54-55 0.19622414454696724 1103 56-57 0.2275485371899464 1103 58-59 0.2776322887205822 1103 60-61 0.152576200782093 1103 62-63 0.15950016072962114 1103 64-65 0.3366754260477052 1103 66-67 0.30801739152412466 1103 68-69 0.5728970860225004 1103 70-71 0.7098397382512331 1103 72-73 0.7930252307791434 1103 74-75 0.5442485822902086 1103 76-77 0.6994059609460876 1103 78-79 0.6630999404996629 1103 80-81 0.5979556530784365 1103 82-83 0.5485703905043096 1103 84-85 0.40974099407993236 1103 86-87 0.5347465400281166 1103 88-89 0.4755893842472432 1103 90-91 0.6272438067748709 1103 92-93 0.6563266078921544 1103 94-95 0.49028638069433583 1103 96-97 0.21084034168961807 1103 98-99 0.33877874230164196 1103 100-101 0.5102412173893072 1103 102-103 0.4752516552837278 1103 104-105 0.37129743179146857 1103 106-107 0.36576473144561916 1103 108-109 0.5407604205371044 1103 110-111 0.6656411525527162 1103 112-113 0.5814026000639032 1103 114-115 0.6412663422957365 1103 116-117 0.6458764594262973 1103 118-119 0.636985356866834 1103 120-121 0.4437888544632749 1103 122-123 0.5074686867063143 1103 124-125 0.19082389621408424 1104 1 0.004816447789188771 1104 2 0.07899689245549268 1104 3 0.007095221628993897 1104 4 0.11814677830385989 1104 5 0.06003375032367586 1104 6 0.09677406226881402 1104 7 0.008026379565414743 1104 8 -0.024360027200437173 1104 9 -0.009835449805734697 1104 10-11 -0.054467768919842285 1104 12-13 0.136432783866816 1104 14-15 0.08155189606284807 1104 16-17 0.11426731738760765 1104 18-19 0.1970628622992905 1104 20-21 0.06820045347366488 1104 22-23 0.11863068105517272 1104 24-25 0.20358132449487698 1104 26-27 0.18414871786695386 1104 28-29 0.2938440706645693 1104 30-31 0.25700847204494437 1104 32-33 0.3483448715723725 1104 34-35 0.32504125293176145 1104 36-37 0.20955337223886517 1104 38-39 0.17024982491813034 1104 40-41 0.12074789641319228 1104 42-43 0.4704470454672105 1104 44-45 0.6717095520510199 1104 46-47 0.6369669037544412 1104 48-49 0.5469349865230697 1104 50-51 0.24168669910459073 1104 52-53 0.4460888213948948 1104 54-55 0.20586695661310017 1104 56-57 0.2303252631775834 1104 58-59 0.2923236934914968 1104 60-61 0.29258629796751023 1104 62-63 0.3397917169583238 1104 64-65 0.45649746315486794 1104 66-67 0.46722476246946343 1104 68-69 0.2852976918536285 1104 70-71 0.3441575471619984 1104 72-73 0.44984714167147644 1104 74-75 0.5825610901786433 1104 76-77 0.5598313048814703 1104 78-79 0.3051294747669928 1104 80-81 0.47492775960373734 1104 82-83 0.5572729021791787 1104 84-85 0.43428977610694375 1104 86-87 0.26001474651555867 1104 88-89 0.2204145767248349 1104 90-91 0.32726904973839765 1104 92-93 0.38809487748700633 1104 94-95 0.3717264917633756 1104 96-97 0.39738584213137074 1104 98-99 0.43095973435011103 1104 100-101 0.5424891032911106 1104 102-103 0.4727336696722162 1104 104-105 0.2943884326749746 1104 106-107 0.2536796626585449 1104 108-109 0.15528774604511852 1104 110-111 0.2862394107882338 1104 112-113 0.3700685094416656 1104 114-115 0.12833563423060923 1104 116-117 0.20649743632898776 1104 118-119 0.24053830398282372 1104 120-121 0.10453604618358625 1104 122-123 0.24961181430949608 1104 124-125 0.32120380281511984 1105 1 0.057156334837202394 1105 2 0.1974793052223518 1105 3 0.14919912808037594 1105 4 0.2183155032587507 1105 5 0.08706489413826546 1105 6 0.014590948824711347 1105 7 0.12190400710507987 1105 8 0.11231890327082539 1105 9 0.06945356198158237 1105 10-11 -0.010628573175459621 1105 12-13 0.04771743692612773 1105 14-15 0.028907366735168694 1105 16-17 -0.08445904354319822 1105 18-19 0.002630785810644909 1105 20-21 0.07713467417527653 1105 22-23 0.12536796223999147 1105 24-25 0.15985929885805206 1105 26-27 0.20830333554088298 1105 28-29 0.20277360456187665 1105 30-31 0.1937600479828916 1105 32-33 0.14081903556365205 1105 34-35 0.0488478753862438 1105 36-37 0.1914037225774834 1105 38-39 0.08159892153660309 1105 40-41 0.3553751783010384 1105 42-43 0.2895598334127385 1105 44-45 0.3714494344122272 1105 46-47 0.32389423485373214 1105 48-49 0.008954296156794328 1105 50-51 0.17048242473906328 1105 52-53 0.12136944380250725 1105 54-55 0.21903687670308614 1105 56-57 0.19126074931635628 1105 58-59 0.10831975657588089 1105 60-61 -0.041840623036414115 1105 62-63 0.1298756107553345 1105 64-65 0.2762605451973741 1105 66-67 0.232767377237586 1105 68-69 0.25086725574649194 1105 70-71 0.17238616947587104 1105 72-73 -0.05882344628812319 1105 74-75 -0.19205634942017014 1105 76-77 -0.3149548596722269 1105 78-79 -0.23389988806196982 1105 80-81 -0.17080256167670171 1105 82-83 -0.023428009404256045 1105 84-85 -0.13383292442969008 1105 86-87 -0.08798047294405364 1105 88-89 0.06779465311974775 1105 90-91 -0.013684246256737254 1105 92-93 -0.06563464703670263 1105 94-95 -0.046189989383954355 1105 96-97 0.12060661404803596 1105 98-99 0.20695978121815273 1105 100-101 0.2604840884105073 1105 102-103 0.011733739974282287 1105 104-105 0.19115570940733306 1105 106-107 -0.109799586515404 1105 108-109 0.002626812902377651 1105 110-111 0.05857789179495754 1105 112-113 0.029759649038133773 1105 114-115 -0.13486943689164832 1105 116-117 -0.3200415930338423 1105 118-119 -0.20425699983926648 1105 120-121 -0.25380529377376604 1105 122-123 0.06310043709205004 1105 124-125 -0.14068076119491124 1106 1 -0.013280875594340102 1106 2 -0.07612621402826392 1106 3 0.016255595193491956 1106 4 -0.021917567734838883 1106 5 0.19133988553972614 1106 6 0.09954451671271158 1106 7 0.049069321609295 1106 8 -0.06406242677146423 1106 9 0.11396364756233623 1106 10-11 0.05365815270311458 1106 12-13 -0.06016894096450187 1106 14-15 -0.025389879709081242 1106 16-17 0.09253935814009395 1106 18-19 0.20537422563099028 1106 20-21 0.21541435936757836 1106 22-23 0.12537807816853785 1106 24-25 0.06590761253276867 1106 26-27 0.1553717394496843 1106 28-29 0.004041130408076299 1106 30-31 0.15411066606613133 1106 32-33 0.27935161896947847 1106 34-35 0.22198705033111565 1106 36-37 0.2469098224823938 1106 38-39 0.29784712595929363 1106 40-41 0.5238155044385735 1106 42-43 0.5940897462134416 1106 44-45 0.49801993231822905 1106 46-47 0.9594679091612974 1106 48-49 0.9785002932838722 1106 50-51 0.7343695714402614 1106 52-53 0.7801743480046639 1106 54-55 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-0.8997261621178723 2216 124-125 -1.558359567765013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 17.0 3 4.0 4 5.0 5 25.0 6 50.0 7 187.0 8 265.0 9 506.0 10 1678.0 11 1949.0 12 1103.0 13 1241.0 14 2063.0 15 2158.0 16 780.0 17 470.0 18 396.0 19 396.0 20 345.0 21 374.0 22 409.0 23 429.0 24 475.0 25 557.0 26 731.0 27 1002.0 28 1143.0 29 1460.0 30 1889.0 31 2314.0 32 3109.0 33 4238.0 34 6213.0 35 10758.0 36 22307.0 37 69818.0 38 66103.0 39 655.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.78565922842718 20.392049177168843 15.335587929240374 22.486703665163603 2 14.356860062999106 20.517093564265828 46.52975118243731 18.596295190297752 3 18.351138126017474 30.278583194459163 30.804539018023135 20.565739661500228 4 10.771979847992988 18.723930989972164 39.00598202502625 31.498107137008603 5 8.96410018955563 36.38455193968947 34.025476662470396 20.625871208284497 6 22.694608471163942 34.79400063577078 26.652281550124744 15.859109342940537 7 24.745450867441793 32.51630366724143 25.84408203369585 16.894163431620925 8 24.615888489659092 35.98318097311461 23.26728381385402 16.133646723372284 9 24.96459912726012 20.35526100316922 26.797256552773792 27.88288331679687 10-11 22.226690813112292 24.46850526437468 34.32439722187437 18.98040670063866 12-13 27.111770428952614 23.905221989962527 31.86608355569256 17.1169240253923 14-15 19.856758917648417 31.060533084162568 28.971640770245926 20.11106722794309 16-17 17.679244010750306 30.180327710936222 34.77738389958675 17.363044378726723 18-19 19.3936577048675 25.870572482684878 37.72769745017388 17.008072362273747 20-21 19.650357397452943 27.2168757104599 33.81403772421633 19.318729167870835 22-23 17.712123802803674 30.841848468720574 34.36510363860987 17.080924089865885 24-25 18.975590255367926 27.96765275356176 37.150446484476596 15.906310506593712 26-27 16.348701004710485 29.986706611052778 35.76523682461396 17.899355559622776 28-29 16.306316286328038 31.26739940854052 32.658870447255104 19.767413857876335 30-31 17.606756509425782 27.564997928928538 35.65927502865785 19.168970532987835 32-33 16.803132616004085 28.83389043550298 37.5193861922147 16.84359075627824 34-35 17.54631012127809 29.593925499224554 35.52778607276685 17.331978306730498 36-37 16.98519424723777 29.639200084769435 33.909219639537234 19.46638602845556 38-39 18.938262804519752 30.915317259249985 32.024303782836114 18.12211615339415 40-41 21.51578349115219 26.23397327836164 35.52826771729393 16.721975513192245 42-43 17.53306489678358 30.306518577029408 34.76341620830162 17.39700031788539 44-45 17.192783038406333 30.96613075685621 32.500168575584475 19.34091762915298 46-47 20.7812755873655 28.21329146236911 31.99925826742831 19.00617468283708 48-49 18.741270192946796 29.310236872778418 32.06283534500197 19.885657589272814 50-51 16.343402914912676 31.590342063943126 32.68704665208889 19.379208369055302 52-53 16.754727341033224 29.55756133743052 33.747387078440624 19.940324243095624 54-55 17.932107387463756 28.205103505408868 32.81395998497269 21.048829122154686 56-57 19.508770746838003 29.576827118513453 29.103129726136924 21.811272408511623 58-59 19.118157035381607 31.200932463804413 28.71949986032309 20.961410640490893 60-61 18.126691776401344 27.018813035227478 35.76644093593164 19.08805425243953 62-63 18.79087957923534 26.882507634065757 34.43324888499292 19.893363901705985 64-65 20.147431389737118 29.467252988604294 30.011270481933515 20.374045139725077 66-67 19.60245060735375 31.896186338634635 29.08049243336448 19.420870620647136 68-69 21.234984731868494 26.95282773501845 33.674177110325495 18.138010422787566 70-71 22.36781265954475 26.81700397838379 32.195287589947114 18.61989577212434 72-73 19.571866179884598 30.49267418674322 30.74529674119313 19.190162892179057 74-75 19.272764928572116 30.36070358632515 33.083440097870174 17.283091387232567 76-77 19.63014516766046 27.972950843359566 34.18086715280654 18.21603683617343 78-79 18.7070734315246 27.31743264201289 33.7064472936394 20.269046632823112 80-81 19.015566751115006 29.189825741010107 33.01456493049869 18.780042577376193 82-83 18.512007398059936 28.583676103688433 34.71501093333077 18.189305564920865 84-85 18.29141420466039 29.2726685996667 33.9530492915009 18.482867904172004 86-87 16.23551454084827 32.314494610397745 30.19188717958598 21.25810366916801 88-89 17.83842752694801 29.051352939476548 32.58903199082949 20.521187542745952 90-91 19.024614621388544 28.33824212926951 34.662762106824715 17.974381142517238 92-93 17.371473157950508 30.985637360202677 33.580015605282675 18.06287387656414 94-95 17.285184471630863 31.85410846739235 31.89264040073211 18.968066660244677 96-97 20.246168517787133 30.690870909633855 31.03187523480171 18.031085337777306 98-99 19.947885183642114 28.20437485098871 34.45815281393517 17.389587151434 100-101 17.96803317575492 28.879716214361228 34.291005507618436 18.86124510226542 102-103 17.499632745163677 30.796666048554698 32.33359743571837 19.37010377056326 104-105 18.674967187255408 29.7419533517165 32.18972389129047 19.39335556973762 106-107 18.101596292978044 28.56323998343015 32.60840245464967 20.72676126894213 108-109 17.144941985637292 29.88931755458263 32.160282437711025 20.80545802206906 110-111 16.443816138681104 30.420782898951416 32.34527702985844 20.790123932509044 112-113 18.47741531495731 27.871102922582274 31.912382741471284 21.739099020989126 114-115 18.667318525226204 29.337729364702824 28.65111036720028 23.3438417428707 116-117 18.633891888507314 29.844089413781976 27.497242545239114 24.024776152471595 118-119 18.047504714107163 27.615877931717094 31.722389058936574 22.614228295239172 120-121 17.77638100192177 26.39376556321374 33.046512636005026 22.78334079885946 122-123 18.832723244388788 28.748675464791447 30.563288700510547 21.85531259030922 124-125 20.71366300772087 29.436853081847037 28.828768079992678 21.02071583043941 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 91.0 1 74.5 2 443.0 3 551.5 4 162.0 5 55.5 6 75.5 7 147.5 8 231.5 9 245.0 10 191.5 11 143.0 12 158.0 13 194.5 14 223.5 15 279.5 16 330.0 17 368.0 18 426.5 19 494.5 20 580.0 21 638.5 22 719.0 23 860.0 24 993.5 25 1120.0 26 1266.0 27 1444.0 28 1649.0 29 1971.0 30 2527.0 31 3483.5 32 5237.5 33 8261.0 34 12356.5 35 16640.0 36 19863.0 37 20381.0 38 17728.5 39 13386.5 40 9673.5 41 7212.5 42 5403.5 43 4252.0 44 3548.5 45 3179.0 46 3005.0 47 3245.0 48 4149.0 49 4096.5 50 3428.5 51 3128.0 52 2693.5 53 2413.0 54 2310.0 55 2192.0 56 1989.5 57 1637.5 58 1209.5 59 888.5 60 626.0 61 435.5 62 283.0 63 186.0 64 134.0 65 88.5 66 56.5 67 48.5 68 43.5 69 26.0 70 19.0 71 20.5 72 17.0 73 13.0 74 8.5 75 5.5 76 2.5 77 2.5 78 2.0 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02215564824536899 2 0.0 3 0.0 4 0.0 5 0.14208513548660545 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.004816445270732389 22-23 7.224667906098582E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 7.224667906098582E-4 92-93 0.0 94-95 9.632890541464778E-4 96-97 0.0 98-99 0.001685755844756336 100-101 2.4082226353661944E-4 102-103 2.4082226353661944E-4 104-105 0.002167400371829575 106-107 0.007706312433171822 108-109 4.816445270732389E-4 110-111 0.00433480074365915 112-113 0.0069838456425619635 114-115 0.005057267534269008 116-117 0.0014449335812197165 118-119 2.4082226353661944E-4 120-121 4.816445270732389E-4 122-123 9.632890541464778E-4 124-125 0.0014449335812197165 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 207622.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.751555455023365 #Duplication Level Percentage of deduplicated Percentage of total 1 85.1288925989312 54.270993173454904 2 9.214169136113746 11.748352293058407 3 2.395275168193463 4.581075531453779 4 1.0245460689538775 2.6126562212455724 5 0.5052974593383791 1.6106749500146542 6 0.34018975278547914 1.30125755339605 7 0.22220232687175423 0.9916020774659908 8 0.1608939399731318 0.820579114925945 9 0.11645477330907471 0.6681755644754086 >10 0.7401104740908871 8.942760059253068 >50 0.09769390806790765 4.381715690276854 >100 0.050327164762255454 6.187246778732644 >500 0.0039472286088043495 1.882910992246752 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 988 0.47586479274835997 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 797 0.3838706880773714 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 677 0.32607334482858275 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 531 0.2557532438758898 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 480 0.23118937299515466 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 447 0.21529510360173776 No Hit ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT 427 0.20566221306027302 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 359 0.17291038521929275 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 346 0.16664900636734065 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 344 0.16568571731319418 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 342 0.1647224282590477 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA 341 0.16424078373197445 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 334 0.1608692720424618 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 299 0.14401171359489842 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 255 0.1228193544036759 No Hit GGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTC 245 0.11800290913294353 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 234 0.1127048193351379 No Hit GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA 232 0.11174153028099142 No Hit TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT 231 0.11125988575391818 No Hit TTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTT 218 0.10499850690196606 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.4082226353661944E-4 0.0 24-25 0.0 0.0 0.0 4.816445270732389E-4 0.0 26-27 0.0 0.0 0.0 0.0014449335812197167 0.0 28-29 0.0 0.0 0.0 0.002167400371829575 0.0 30-31 0.0 0.0 0.0 0.00433480074365915 0.0 32-33 0.0 0.0 0.0 0.007706312433171821 0.0 34-35 0.0 0.0 0.0 0.01228193544036759 0.0 36-37 0.0 0.0 0.0 0.02143318145475913 0.0 38-39 0.0 0.0 0.0 0.040698962537688683 0.0 40-41 0.0 0.0 0.0 0.06622612247257034 0.0 42-43 0.0 0.0 0.0 0.08717765940025624 0.0 44-45 0.0 0.0 0.0 0.11174153028099142 0.0 46-47 0.0 0.0 0.0 0.13799115700648296 0.0 48-49 0.0 0.0 0.0 0.1748369633275857 0.0 50-51 0.0 0.0 0.0 0.22203812698076314 0.0 52-53 0.0 0.0 0.0 0.2757414917494293 0.0 54-55 0.0 0.0 0.0 0.34846981533748833 0.0 56-57 0.0 0.0 0.0 0.4303493849399389 0.0 58-59 0.0 0.0 0.0 0.5093390873799502 0.0 60-61 0.0 0.0 0.0 0.5965167467802064 0.0 62-63 0.0 0.0 0.0 0.6909190740865612 0.0 64-65 0.0 0.0 0.0 0.7939910028802343 0.0 66-67 0.0 0.0 0.0 0.909344867114275 0.0 68-69 0.0 0.0 0.0 1.0625078267235648 0.0 70-71 0.0 0.0 0.0 1.213744208224562 0.0 72-73 0.0 0.0 0.0 1.3734093689493405 0.0 74-75 0.0 0.0 0.0 1.5487279768039994 0.0 76-77 0.0 0.0 0.0 1.7365693423625626 0.0 78-79 0.0 0.0 0.0 1.9362109988344203 0.0 80-81 0.0 0.0 0.0 2.1218849640211537 0.0 82-83 0.0 0.0 0.0 2.333086089142769 0.0 84-85 0.0 0.0 0.0 2.564757106664997 0.0 86-87 0.0 0.0 0.0 2.807746770573446 0.0 88-89 0.0 0.0 0.0 3.071447149146044 0.0 90-91 0.0 0.0 0.0 3.3204573696429085 0.0 92-93 0.0 0.0 0.0 3.5723574573022123 0.0 94-95 0.0 0.0 0.0 3.847376482261032 0.0 96-97 0.0 0.0 0.0 4.115652483840826 0.0 98-99 0.0 0.0 0.0 4.42246004758648 0.0 100-101 0.0 0.0 0.0 4.761296972382503 0.0 102-103 0.0 0.0 0.0 5.074606737243645 0.0 104-105 0.0 0.0 0.0 5.393455414166128 0.0 106-107 0.0 0.0 0.0 5.770342256600938 0.0 108-109 0.0 0.0 0.0 6.145543343190991 0.0 110-111 0.0 0.0 0.0 6.524838408261167 0.0 112-113 0.0 0.0 0.0 6.960485882998912 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 25 1.1906195E-7 119.21453 2 ATAACGC 30 3.5297853E-7 99.34544 3 TCACGCA 25 1.7070302E-5 95.37163 4 CACGCAG 25 1.7070302E-5 95.37163 5 ATCACGC 25 1.7070302E-5 95.37163 3 ACCTGGG 520 0.0 87.11831 3 CTGGGGG 90 0.0 86.09938 5 TAACGCA 35 8.83716E-7 85.15324 4 GTACCTG 560 0.0 83.02441 1 TACCTGG 565 0.0 82.28967 2 CCTGGGG 305 0.0 82.08213 4 CGGACCT 30 4.2181164E-5 79.47636 5 GACGGAC 25 0.0020398365 71.52872 3 CTGGGGA 210 0.0 65.28414 5 CCTGGGA 155 0.0 61.53008 4 ATGACGT 40 1.7528977E-4 59.60726 5 TGACGTG 40 1.7697005E-4 59.49241 6 TGGGGAG 165 0.0 50.478413 6 TGTATAG 195 0.0 48.908524 2 GTAAGAC 75 1.4468715E-6 47.685806 3 >>END_MODULE