FastQCFastQC Report
Fri 27 May 2016
SRR939987_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939987_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815871
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT83390.45922865666118357No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT46460.2558551791399279No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36000.19825196833916067No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27210.14984544606968228No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25860.14241099725696374No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT23310.12836814949960654No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC21070.1160324714696143No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA20930.1152614915927398No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19170.10556917314060305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC2650.03089.2793108-109
CGTCGCT207.561357E-73061.9133110-111
CGTCGAC108.718749E-43061.9133110-111
CGGCGCG600.02480.7844108-109
CGTCGCG550.02255.2588108-109
CCGCGCC2200.02142.4958108-109
CCGCGCG700.02126.387108-109
GTCGACG150.00196150622041.2755110-111
CCGTTCG557.2759576E-121804.207108-109
CCGCTCG750.01653.8566108-109
GTCCGCG200.00348674341530.9567110-111
CAGCGCG651.6370905E-111526.6368108-109
CTCCGCC2200.01391.7788110-111
CGTCGTT953.8198777E-111289.2267110-111
CGCGTCG200.0053130741240.3925108-109
CCGCGCT602.309207E-81240.3922108-109
CGCGAAT250.0054474461224.7654110-111
CGCCGCT250.0054474461224.7654110-111
TCGTTCG451.6198332E-51102.571108-109
CCGCACG451.6198332E-51102.571108-109
Produced by FastQC (version 0.11.5)