##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939987_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1815871 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.110942352182505 34.0 31.0 34.0 30.0 34.0 2 32.15310448814921 34.0 31.0 34.0 30.0 34.0 3 32.299838479715795 34.0 31.0 34.0 30.0 34.0 4 35.52572567104161 37.0 35.0 37.0 35.0 37.0 5 35.58311796377606 37.0 37.0 37.0 35.0 37.0 6 35.63256751167897 37.0 37.0 37.0 35.0 37.0 7 35.58517262514793 37.0 37.0 37.0 35.0 37.0 8 35.56569767345808 37.0 37.0 37.0 35.0 37.0 9 37.23381396585991 39.0 39.0 39.0 35.0 39.0 10-11 37.22885105825249 39.0 39.0 39.0 35.0 39.0 12-13 37.17885962163612 39.0 39.0 39.0 35.0 39.0 14-15 38.721911688660704 41.0 40.0 41.0 36.0 41.0 16-17 38.741351120206225 41.0 40.0 41.0 35.5 41.0 18-19 38.77319974822 41.0 40.0 41.0 35.0 41.0 20-21 38.84948132328783 41.0 40.0 41.0 35.0 41.0 22-23 38.85179646571811 41.0 39.5 41.0 35.0 41.0 24-25 38.88444305790444 41.0 39.0 41.0 35.0 41.0 26-27 38.86062638810797 41.0 39.0 41.0 35.0 41.0 28-29 38.82696265318407 41.0 39.0 41.0 34.5 41.0 30-31 38.76355423926039 41.0 39.0 41.0 34.0 41.0 32-33 38.67050853281979 41.0 39.0 41.0 34.0 41.0 34-35 38.60348422327357 40.5 39.0 41.0 34.0 41.0 36-37 38.784035870389474 41.0 39.0 41.0 35.0 41.0 38-39 38.75403511593059 41.0 39.0 41.0 34.0 41.0 40-41 38.707978705535794 41.0 39.0 41.0 34.0 41.0 42-43 38.65089948570135 41.0 39.0 41.0 34.0 41.0 44-45 38.569508516849496 41.0 39.0 41.0 34.0 41.0 46-47 38.49149499055825 41.0 39.0 41.0 33.0 41.0 48-49 38.42233644350287 41.0 39.0 41.0 33.0 41.0 50-51 38.32539150633498 41.0 39.0 41.0 33.0 41.0 52-53 38.25888760820565 40.5 38.5 41.0 33.0 41.0 54-55 38.14315444213824 40.0 38.0 41.0 33.0 41.0 56-57 37.977798808395534 40.0 38.0 41.0 33.0 41.0 58-59 37.850768308982296 40.0 38.0 41.0 32.5 41.0 60-61 37.664487455331354 40.0 37.5 41.0 32.0 41.0 62-63 37.47342487434405 40.0 37.0 41.0 32.0 41.0 64-65 37.233196906608455 40.0 37.0 41.0 31.0 41.0 66-67 36.95015202071072 39.5 36.0 41.0 31.0 41.0 68-69 36.58730796405692 39.0 36.0 41.0 31.0 41.0 70-71 36.19393282892892 39.0 35.0 40.5 30.5 41.0 72-73 35.80169626586911 38.0 35.0 40.0 30.0 41.0 74-75 35.38435054031922 37.0 35.0 39.5 30.0 41.0 76-77 34.91147581518731 37.0 35.0 39.0 30.0 41.0 78-79 34.41267634099559 36.0 34.5 39.0 29.0 40.0 80-81 33.927861065020586 36.0 34.0 37.5 29.0 39.0 82-83 33.66856566353006 35.5 34.0 37.0 29.0 39.0 84-85 33.19904855576195 35.0 34.0 37.0 29.0 39.0 86-87 32.52931045211912 35.0 34.0 36.5 28.5 37.5 88-89 16.702853892154234 18.0 17.0 18.5 2.0 19.5 90-91 2.851862825057507 2.0 2.0 2.0 2.0 2.0 92-93 2.3285814355755448 2.0 2.0 2.0 2.0 2.0 94-95 2.112036592907756 2.0 2.0 2.0 2.0 2.0 96-97 2.000014868897625 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.13535207393839244 1101 2 0.15945047576305882 1101 3 0.1803753918628459 1101 4 0.20652749027360784 1101 5 0.16826429339657523 1101 6 0.1643980904323996 1101 7 0.13260859229836797 1101 8 0.0791546113247108 1101 9 0.20218126628355293 1101 10-11 0.14454157986853033 1101 12-13 0.17315750961585508 1101 14-15 0.2670091273424475 1101 16-17 0.3497880853552999 1101 18-19 0.28434565755551944 1101 20-21 0.2285805556111029 1101 22-23 0.32338113793394285 1101 24-25 0.3276942843016357 1101 26-27 0.28282367591499025 1101 28-29 0.3408541307248498 1101 30-31 0.25913053352270765 1101 32-33 0.34926184675111926 1101 34-35 0.2562430938529303 1101 36-37 0.3309275665029574 1101 38-39 0.2631088885979054 1101 40-41 0.3439486009903234 1101 42-43 0.3083575577755582 1101 44-45 0.30680134295788264 1101 46-47 0.2855521571484303 1101 48-49 0.32115412335949856 1101 50-51 0.3004125574529084 1101 52-53 0.30137668010358 1101 54-55 0.3007065692361337 1101 56-57 0.3464764637552449 1101 58-59 0.294754313231941 1101 60-61 0.2640985732360761 1101 62-63 0.26536759160282486 1101 64-65 0.16158265022573204 1101 66-67 0.22309750480244617 1101 68-69 0.27640420163410084 1101 70-71 0.24690731408733768 1101 72-73 0.1301186068642437 1101 74-75 0.1399796184788613 1101 76-77 0.06872281164218208 1101 78-79 0.11795042285182689 1101 80-81 0.1459301585534405 1101 82-83 0.1737982459172116 1101 84-85 0.2454758391798677 1101 86-87 0.33518739585772295 1101 88-89 0.8717743969098102 1101 90-91 -0.10037227607822885 1101 92-93 -0.22913754148144028 1101 94-95 -0.04122394132043539 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.05277935086718344 1102 2 0.02064930412831245 1102 3 0.011713872240946444 1102 4 -0.009166728451972972 1102 5 0.020034274065700686 1102 6 5.027583553243176E-4 1102 7 0.046635611534199484 1102 8 0.013796778079218086 1102 9 0.10257895914652693 1102 10-11 0.04829464425580454 1102 12-13 0.04129345883559665 1102 14-15 0.06669053317846618 1102 16-17 0.1201623628040025 1102 18-19 -0.004868877439179187 1102 20-21 0.0680054837192614 1102 22-23 0.027228146754190163 1102 24-25 0.07297055241401296 1102 26-27 0.015346673056598092 1102 28-29 0.13683125101378124 1102 30-31 0.08088503221212306 1102 32-33 0.12813031904654792 1102 34-35 0.09384694526048776 1102 36-37 0.04883160022241384 1102 38-39 0.09087410292239184 1102 40-41 0.005949177538937533 1102 42-43 0.03812977394515116 1102 44-45 0.08115639793030027 1102 46-47 0.12927936325198885 1102 48-49 0.057196030745686244 1102 50-51 0.10480846568584923 1102 52-53 0.05798503471710603 1102 54-55 0.09912805140594116 1102 56-57 0.033654776730024594 1102 58-59 0.0837691770321527 1102 60-61 0.040276040239660915 1102 62-63 0.03168749124880321 1102 64-65 0.0018114473363937122 1102 66-67 0.05186547383534901 1102 68-69 0.10743107869650004 1102 70-71 0.06992456420182691 1102 72-73 0.009300489099075548 1102 74-75 0.01346717987460977 1102 76-77 0.05042100651089498 1102 78-79 0.014600392649796845 1102 80-81 -0.0513754585538706 1102 82-83 0.021615412455709304 1102 84-85 -0.0632353076098795 1102 86-87 0.03840923432514387 1102 88-89 1.1507702754512827 1102 90-91 0.19434460634905681 1102 92-93 -0.003140226281372893 1102 94-95 0.11767262004625234 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.011317867321011477 1103 2 0.04754303067596055 1103 3 0.0274497867756196 1103 4 0.04114807015631072 1103 5 0.05266329423128013 1103 6 -0.020297489022851778 1103 7 -0.05744949545346145 1103 8 0.019340081770216955 1103 9 -0.012073471124679713 1103 10-11 -0.05322871781371674 1103 12-13 -0.12783839144376685 1103 14-15 -0.14621100456353986 1103 16-17 -0.05483359814805766 1103 18-19 -0.11975631388402519 1103 20-21 -0.09503327353287716 1103 22-23 -0.08405920826097457 1103 24-25 -0.04152355953448961 1103 26-27 -0.06517340803944904 1103 28-29 -0.06229095497673143 1103 30-31 0.009631592843099668 1103 32-33 0.04381306951616892 1103 34-35 0.008318444718803164 1103 36-37 -0.09766289230789482 1103 38-39 -0.01822243542046209 1103 40-41 -0.03432522750081546 1103 42-43 -0.014164930438241186 1103 44-45 -0.017637092885223637 1103 46-47 -0.0880462834822211 1103 48-49 -0.12839999835275506 1103 50-51 -0.15426834851083981 1103 52-53 -0.10079403291308608 1103 54-55 -0.12729858202984445 1103 56-57 -0.052708022758039874 1103 58-59 -0.06635899130362333 1103 60-61 -0.05775389288976385 1103 62-63 -0.16430418228862465 1103 64-65 -0.08984374060831612 1103 66-67 -0.1276695892711075 1103 68-69 -0.14036065100145834 1103 70-71 -0.16005280168401725 1103 72-73 -0.17073895301495412 1103 74-75 -0.20438678668670462 1103 76-77 -0.18069910875102124 1103 78-79 -0.2411410020157021 1103 80-81 -0.2226039067964436 1103 82-83 -0.1382623071055491 1103 84-85 -0.07694304858813439 1103 86-87 -0.1270941144549056 1103 88-89 0.5437216811480852 1103 90-91 -0.5911628726085074 1103 92-93 -0.35418724327070494 1103 94-95 -0.12452414012759228 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 0.08066604099646213 1104 2 0.11732388540083605 1104 3 0.22233928726671337 1104 4 0.13596805529647327 1104 5 0.13764723095287934 1104 6 0.12419951135237994 1104 7 0.17066400741759935 1104 8 0.16935376059733187 1104 9 0.24908987201057187 1104 10-11 0.24544742586330415 1104 12-13 0.3282533209809415 1104 14-15 0.2887912453419048 1104 16-17 0.24672234974981677 1104 18-19 0.2513581143634269 1104 20-21 0.20167924834950668 1104 22-23 0.09540976920558109 1104 24-25 0.15421014135814204 1104 26-27 0.2399835845321192 1104 28-29 0.17909048696598973 1104 30-31 0.21209572516445263 1104 32-33 0.12640358523037776 1104 34-35 0.17664306496316584 1104 36-37 0.2567762982045281 1104 38-39 0.181210603357151 1104 40-41 0.14888038694761718 1104 42-43 0.11713738360072057 1104 44-45 0.07193672569017195 1104 46-47 0.0422289225597865 1104 48-49 0.13588181837487667 1104 50-51 0.07786486084098954 1104 52-53 0.1182403166399979 1104 54-55 0.13131070731397898 1104 56-57 0.07006229104770512 1104 58-59 0.0836085681992742 1104 60-61 0.06592737100396562 1104 62-63 0.020376353562610916 1104 64-65 -0.07871590262541872 1104 66-67 0.05041999433941413 1104 68-69 0.03450416780267318 1104 70-71 0.08953795471774129 1104 72-73 0.16164189543111718 1104 74-75 0.12701838017255795 1104 76-77 0.00796308700720516 1104 78-79 0.024023734416473985 1104 80-81 0.08603408566001747 1104 82-83 0.047233352296146336 1104 84-85 0.03900377291588342 1104 86-87 0.03579560130953041 1104 88-89 0.6646964059644667 1104 90-91 -0.726547157953326 1104 92-93 -0.35418724327070494 1104 94-95 -0.12452414012759228 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.10999842195629839 1105 2 -0.10775308254808635 1105 3 -0.10134595613205022 1105 4 -0.06967518070616308 1105 5 -0.14604420589019185 1105 6 -0.20666292576795087 1105 7 -0.12934211400992268 1105 8 -0.2410641888684779 1105 9 -0.19483101033919326 1105 10-11 -0.16935058503283784 1105 12-13 -0.14277252224569992 1105 14-15 -0.2106012929980352 1105 16-17 -0.14464643745135675 1105 18-19 -0.09560439729429504 1105 20-21 -0.11600915658651445 1105 22-23 -0.2317236383050485 1105 24-25 -0.09619477306790003 1105 26-27 -0.11331100376586534 1105 28-29 -0.16713709330573323 1105 30-31 -0.22226096681612262 1105 32-33 -0.18029901781665103 1105 34-35 -0.07095679532424981 1105 36-37 -0.25414504865356236 1105 38-39 -0.18613014050431786 1105 40-41 -0.09938274801545788 1105 42-43 -0.03765263343125724 1105 44-45 -0.019729490871526423 1105 46-47 0.06705635231639917 1105 48-49 0.09114210507373599 1105 50-51 0.06193906694585394 1105 52-53 0.06316115217884999 1105 54-55 0.011456757372847903 1105 56-57 0.021259030127254164 1105 58-59 -0.09089712652873772 1105 60-61 -0.056317536508473154 1105 62-63 -0.09188774642551323 1105 64-65 -0.08826028837617628 1105 66-67 0.04992523896246581 1105 68-69 0.0023943998047712967 1105 70-71 -0.03675717730703809 1105 72-73 0.030949778029338404 1105 74-75 0.14444248347345479 1105 76-77 0.12806693522460222 1105 78-79 0.07461121141611216 1105 80-81 -0.029052712581332685 1105 82-83 -0.019856773121105675 1105 84-85 0.06758723858007443 1105 86-87 0.17538575373307452 1105 88-89 0.855046515610038 1105 90-91 -0.8548454371120258 1105 92-93 -0.35418724327070494 1105 94-95 -0.12452414012759228 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.1365880221899367 1106 2 -0.14724353643744337 1106 3 -0.06198982923156393 1106 4 -0.13027150009064314 1106 5 -0.08474823759080152 1106 6 -0.14107143529165 1106 7 -0.12738547823565938 1106 8 -0.08943030905261651 1106 9 -0.09017319748792829 1106 10-11 -0.21910598805231984 1106 12-13 -0.2297835646525499 1106 14-15 -0.18299800723942639 1106 16-17 -0.19550734178434936 1106 18-19 -0.25484981298309606 1106 20-21 -0.28513965196457036 1106 22-23 -0.21132043322668181 1106 24-25 -0.25069924444778025 1106 26-27 -0.35106129983851986 1106 28-29 -0.16931349867908807 1106 30-31 -0.14620716878704343 1106 32-33 -0.09325856074070771 1106 34-35 -0.1558127229753623 1106 36-37 -0.05976997380373206 1106 38-39 -0.11180200306217358 1106 40-41 -0.06089330332939369 1106 42-43 -0.415882276554278 1106 44-45 -0.13608355664413807 1106 46-47 -0.28853674195971024 1106 48-49 -0.1375098836730544 1106 50-51 -0.2128095601945006 1106 52-53 -0.25352803071665875 1106 54-55 -0.17409748852957563 1106 56-57 -0.26029636154656544 1106 58-59 -0.14452581115376262 1106 60-61 -0.0864290531218046 1106 62-63 0.004715069398741889 1106 64-65 -0.05332714721868115 1106 66-67 -0.07846811080106164 1106 68-69 -0.16878335654231336 1106 70-71 -0.0684315933383175 1106 72-73 -0.09479246962956722 1106 74-75 -0.20481209978721893 1106 76-77 -0.24201085256816413 1106 78-79 -0.2806348597996049 1106 80-81 -0.2525499906739199 1106 82-83 -0.24483802126955823 1106 84-85 -0.22325177283492081 1106 86-87 -0.29193517921618906 1106 88-89 0.5546284070993224 1106 90-91 -0.2304497509833534 1106 92-93 -0.33224600117327086 1106 94-95 -0.12452414012759228 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.03139741121027839 1107 2 -0.09071027483917504 1107 3 0.023935060046781587 1107 4 0.008581616895291688 1107 5 -0.013613367798079423 1107 6 -0.046913159399075255 1107 7 -0.01635425066537266 1107 8 0.03209630811205244 1107 9 0.06634040902309835 1107 10-11 0.052788117863379114 1107 12-13 0.1007805763821139 1107 14-15 0.11483540969140904 1107 16-17 0.06790578746699794 1107 18-19 0.0929090600611886 1107 20-21 0.05256203451664021 1107 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118-119 0.0 2215 120-121 0.0 2215 122-123 0.0 2215 124-125 0.0 2216 1 -0.005339794537007947 2216 2 -0.046044820683420085 2216 3 -0.11632903246782433 2216 4 -0.08028121761440588 2216 5 -0.03654291380622965 2216 6 0.010390728880395272 2216 7 0.06263312061295068 2216 8 -0.06952305506697343 2216 9 -0.19163547033151218 2216 10-11 -0.09896679467657066 2216 12-13 -0.03367238153860086 2216 14-15 -0.16171823166560984 2216 16-17 -0.15295087919636785 2216 18-19 -0.17277259876323114 2216 20-21 -0.18517315711713422 2216 22-23 -0.2307277981144935 2216 24-25 -0.2185884621602341 2216 26-27 -0.21772308963138443 2216 28-29 -0.2194558641199862 2216 30-31 -0.2915956232248931 2216 32-33 -0.31113779782987194 2216 34-35 -0.25533303534542284 2216 36-37 -0.29866485742165594 2216 38-39 -0.2956752261537545 2216 40-41 -0.3247443780165966 2216 42-43 -0.27747448167033184 2216 44-45 -0.3100668113888716 2216 46-47 -0.30341010129109236 2216 48-49 -0.33928416967527397 2216 50-51 -0.34766556868414966 2216 52-53 -0.29832055329088547 2216 54-55 -0.28818316219992113 2216 56-57 -0.33503006764998133 2216 58-59 -0.3058779436726482 2216 60-61 -0.3327215518454807 2216 62-63 -0.4516239075719284 2216 64-65 -0.4057880257491746 2216 66-67 -0.4489604580938149 2216 68-69 -0.5323822674933112 2216 70-71 -0.5143641833506649 2216 72-73 -0.5054920295327037 2216 74-75 -0.5954363079863398 2216 76-77 -0.5936290690759662 2216 78-79 -0.5223542884069161 2216 80-81 -0.5241725105748216 2216 82-83 -0.5033898711509153 2216 84-85 -0.6416370418238628 2216 86-87 -0.6018148294859955 2216 88-89 -1.3102083299220624 2216 90-91 -0.8548454371120258 2216 92-93 -0.35418724327070494 2216 94-95 -0.12452414012759228 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11455.0 3 2105.0 4 1473.0 5 1231.0 6 1254.0 7 1695.0 8 1997.0 9 2236.0 10 2822.0 11 3534.0 12 4458.0 13 5471.0 14 6354.0 15 7541.0 16 8554.0 17 10101.0 18 13553.0 19 16455.0 20 15572.0 21 23240.0 22 26679.0 23 31809.0 24 49727.0 25 84231.0 26 151743.0 27 452708.0 28 843456.0 29 33015.0 30 1402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.5864733783402 21.293307729458753 15.539870398282698 22.580348493918343 2 12.447332089110443 22.01646713759163 44.31048886504955 21.225711908248382 3 18.080212524956917 28.118567946723317 30.23715131935599 23.564068208963775 4 10.83010611928268 18.84577305206021 42.15135482385169 28.17276600480542 5 11.70432625688228 40.24017802882335 36.01518908950863 12.040306624785742 6 26.99409814904253 39.25124637157595 21.298594448614466 12.456061030767053 7 25.940168657355066 33.13666003807539 24.427836558874503 16.495334745695043 8 23.08137527390437 37.860398673694334 22.584588883241157 16.473637169160142 9 25.14506812433262 18.46628973093353 23.083523003561375 33.305119141172476 10-11 22.660622504965975 27.97954354335171 30.9113823390278 18.44845161265452 12-13 24.86481455679716 25.639525310338634 30.15487606555427 19.340784067309933 14-15 20.333674859344242 29.161346671663356 27.899581990886457 22.605396478105945 16-17 19.41455433486364 30.84423424765449 30.09533475653057 19.645876660951302 18-19 19.97844954588738 30.05883554850119 30.768519043761245 19.19419586185019 20-21 21.733188715992412 29.05321626267708 29.106194892518637 20.107400128811868 22-23 20.390503673190665 30.400443866817927 29.2723557983545 19.93669666163691 24-25 20.892563403457626 29.422987646148872 30.17673061577612 19.507718334617383 26-27 19.65533344604325 30.070583207727864 30.22629360786091 20.047789738367978 28-29 19.46028030949693 30.62992537929895 29.09766775890079 20.81212655230333 30-31 20.309647546549286 29.50077400872639 29.619477374769467 20.57010106995486 32-33 19.736685226391284 30.252011524082807 30.076391242776452 19.934912006749457 34-35 20.181296067806635 30.320295593928968 30.0196407285876 19.478767609676797 36-37 19.639522454538298 29.790282228508776 30.232632420069947 20.33756289688298 38-39 20.31972535493986 30.43258579491605 29.47780982239377 19.76987902775032 40-41 21.205471093486267 29.39261654599914 30.033355893673065 19.368556466841532 42-43 19.64602661752955 30.554758570405056 29.77353567516635 20.02567913689904 44-45 19.62637152039686 30.259358059065967 29.530202635874414 20.584067784662764 46-47 20.66950240408047 29.59576974355557 29.414011237582404 20.320716614781556 48-49 20.146067885991712 29.74819755621003 29.577645511678163 20.528089046120094 50-51 19.372917664810807 30.56559665614829 29.95938585911987 20.10209981992103 52-53 19.58919438660566 30.49800894446797 29.73727208595765 20.175524582968723 54-55 19.60791332536828 29.56262595695621 30.19800385329503 20.631456864380482 56-57 20.319147120032206 29.58808747978243 28.97865542210873 21.114109978076637 58-59 20.8240287994026 29.861950546046494 28.63267820236129 20.68134245218961 60-61 20.05247068762043 29.4036030092446 30.342766639260166 20.2011596638748 62-63 20.049166488148114 29.317776427951102 30.311569489242352 20.32148759465843 64-65 20.10912124766641 30.233799776415715 29.258895185228017 20.39818379068986 66-67 20.396217572724055 30.290395077623906 28.606106931604725 20.707280418047315 68-69 20.632894836475778 29.32570610384872 29.808396836214197 20.233002223461302 70-71 21.463529072274408 29.11663328507366 29.334104680343483 20.085732962308445 72-73 20.683077156912578 29.539873702482172 29.534036283414405 20.24301285719085 74-75 19.913171144866567 30.613435646034326 29.700127376889657 19.773265832209447 76-77 20.309096846637235 29.24464347963044 29.956505720946037 20.489753952786295 78-79 20.16207707127927 29.25521149843504 29.81852445488086 20.764186975404826 80-81 20.73875291802116 29.714060084664606 29.388018201733495 20.159168795580744 82-83 20.214581816029067 29.686332765649286 30.337347506618773 19.761737911702873 84-85 20.19695793201055 29.570178481939784 30.18129051088459 20.05157307516507 86-87 19.517162894192005 30.595167388504724 29.221733837670005 20.665935879633263 88-89 29.545941924778575 18.864236018419934 35.70735259673379 15.882469460067702 90-91 31.04291460377561 16.278375327723463 17.981641513828585 34.69706855467234 92-93 22.46647962626357 18.408705265619357 36.925889660306275 22.19892544781079 94-95 16.35252380656003 7.431480674674799 29.409896060247714 46.80609945851746 96-97 28.242292345979063 7.486634442320003 16.318821342818744 47.952251868882186 98-99 57.4276887399336 14.051654303731118 18.984783264768016 9.53587369156726 100-101 31.26995333813145 7.099310736564039 13.242172930641368 48.38856299466314 102-103 23.682702378290596 2.087917907198418 38.02011132424068 36.20926839027031 104-105 41.407662133060946 4.433321180949003 42.768619697153824 11.390396988836228 106-107 6.185128173614056 1.9783237232475146 7.336539859498396 84.50000824364004 108-109 19.07416189169304 12.276721077996575 23.40388793398169 45.24522909632869 110-111 40.972596640575546 2.811998486371473 26.95134804153989 29.264056831513095 112-113 27.463027537417307 2.780375105830356 40.449380802732215 29.307216554020123 114-115 29.507598657466282 4.016343888761491 25.15103504346557 41.32502241030666 116-117 20.618241066408384 4.430618200025446 21.744240926869107 53.20689980669706 118-119 21.43522595801083 4.4531327299426815 17.050177950933936 57.06146336111255 120-121 26.397455815709442 4.450076979539021 31.47249156874055 37.67997563601099 122-123 34.912722759899395 1.7076403691343747 35.78075710679574 27.59887976417049 124-125 39.165976045739676 2.1157796595973535 21.309329113198647 37.40891518146432 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 6.5 2 19.5 3 28.0 4 39.5 5 48.0 6 43.5 7 38.0 8 34.0 9 40.5 10 49.5 11 55.5 12 71.5 13 90.5 14 111.5 15 162.5 16 241.0 17 363.5 18 552.0 19 874.5 20 1363.0 21 2095.0 22 3290.5 23 4975.0 24 7363.0 25 10548.0 26 14486.5 27 19488.5 28 25776.0 29 33295.0 30 41950.0 31 51868.5 32 62366.5 33 72968.5 34 82773.0 35 90083.5 36 93454.5 37 93159.5 38 91285.0 39 88574.0 40 84972.5 41 80739.0 42 77042.5 43 75403.5 44 74416.5 45 70854.5 46 64731.5 47 58700.0 48 54133.5 49 50084.5 50 45099.0 51 38974.0 52 32371.0 53 26108.5 54 20826.5 55 16519.5 56 12912.5 57 10093.0 58 7728.0 59 5701.5 60 4219.5 61 3059.0 62 2152.0 63 1532.5 64 1060.5 65 673.5 66 472.5 67 341.5 68 244.0 69 171.5 70 123.5 71 91.5 72 60.5 73 46.0 74 36.0 75 25.5 76 19.0 77 15.0 78 12.0 79 9.5 80 4.0 81 4.5 82 5.5 83 4.0 84 2.0 85 2.0 86 3.0 87 2.5 88 1.5 89 3.0 90 5.0 91 3.5 92 1.0 93 0.5 94 2.0 95 3.0 96 3.5 97 5.5 98 4.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 6.608398944638688E-4 3 0.04091700346555455 4 0.04614865263006017 5 0.048681872225505005 6 0.0 7 0.0 8 0.0 9 0.0 10-11 7.709798768745137E-4 12-13 0.0017622397185703168 14-15 8.26049868079836E-5 16-17 3.579549428345956E-4 18-19 0.007269238839102557 20-21 0.0024506146086368473 22-23 6.057699032585464E-4 24-25 0.0 26-27 0.0 28-29 0.0011564698153117704 30-31 0.0 32-33 0.0011840048109144317 34-35 8.26049868079836E-5 36-37 1.101399824106448E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.652099736159672E-4 46-47 0.0 48-49 1.652099736159672E-4 50-51 5.50699912053224E-5 52-53 0.0 54-55 0.001239074802119754 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.50699912053224E-5 66-67 0.0 68-69 4.6809492524524046E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.579549428345956E-4 80-81 0.0 82-83 7.434448812718525E-4 84-85 5.50699912053224E-5 86-87 0.0011289348197091092 88-89 19.236360952953156 90-91 26.231323700857605 92-93 40.29975146912969 94-95 44.69940320650531 96-97 48.4294038508242 98-99 93.06591712737303 100-101 51.718321400584074 102-103 53.20317357345318 104-105 74.84950748153365 106-107 81.6291740988209 108-109 12.613148180680236 110-111 39.022485628109045 112-113 52.029219035933714 114-115 86.79173795935945 116-117 66.45425253225588 118-119 71.89263444374629 120-121 80.38057218822262 122-123 75.88245530657188 124-125 50.029710260255264 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1815871.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.90616816218292 #Duplication Level Percentage of deduplicated Percentage of total 1 84.86300385792042 63.567624377293654 2 10.693838154875923 16.02068878256608 3 2.518372102058835 5.659248125153076 4 0.8426311927924224 2.5247309530403985 5 0.36706613179410197 1.37477586974055 6 0.20264740724721167 0.9107724458934005 7 0.11896730406528302 0.6237969418880946 8 0.07423332514976536 0.4448427149525062 9 0.049389867936292194 0.33296451778295183 >10 0.24085981392759204 3.202462421831868 >50 0.016489921159592233 0.8510982415529379 >100 0.009854430021773632 1.4671063357801142 >500 0.0014702728060234042 0.7618966491976282 >1k 0.0011027046045175475 1.7987941560197767 >5k 7.351364030116982E-5 0.45919746730695266 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8339 0.45922865666118357 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4646 0.2558551791399279 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3600 0.19825196833916067 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2721 0.14984544606968228 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2586 0.14241099725696374 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2331 0.12836814949960654 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2107 0.1160324714696143 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 2093 0.1152614915927398 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1917 0.10556917314060305 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 5.506999120532241E-5 0.0 8 0.0 0.0 0.0 5.506999120532241E-5 0.0 9 0.0 0.0 0.0 5.506999120532241E-5 0.0 10-11 0.0 0.0 0.0 1.1013998241064482E-4 0.0 12-13 0.0 0.0 0.0 1.1013998241064482E-4 0.0 14-15 0.0 0.0 0.0 1.652099736159672E-4 0.0 16-17 0.0 0.0 0.0 1.652099736159672E-4 0.0 18-19 0.0 0.0 0.0 2.2027996482128963E-4 0.0 20-21 0.0 0.0 0.0 3.028849516292732E-4 0.0 22-23 0.0 0.0 0.0 4.13024934039918E-4 0.0 24-25 0.0 0.0 0.0 5.231649164505628E-4 0.0 26-27 0.0 0.0 0.0 7.709798768745136E-4 0.0 28-29 0.0 0.0 0.0 0.001514424758146366 0.0 30-31 0.0 0.0 0.0 0.004240389322809825 0.0 32-33 0.0 0.0 0.0 0.00919668853128884 0.0 34-35 0.0 2.7534995602661204E-5 0.0 0.015502202524298257 0.0 36-37 0.0 5.506999120532241E-5 0.0 0.02624085080933613 0.0 38-39 0.0 5.506999120532241E-5 0.0 0.04460669287631115 0.0 40-41 0.0 5.506999120532241E-5 0.0 0.06927804893629558 0.0 42-43 0.0 8.260498680798362E-5 0.0 0.09518847979839978 0.0 44-45 0.0 1.37674978013306E-4 0.0 0.12456832010643928 0.0 46-47 0.0 1.9274496921862843E-4 0.0 0.15708714991318215 0.0 48-49 0.0 2.2027996482128963E-4 0.0 0.19701289353704088 0.0 50-51 0.0 2.2027996482128963E-4 0.0 0.24109642149690147 0.0 52-53 5.506999120532241E-5 2.2027996482128963E-4 0.0 0.28730014411816696 0.0 54-55 5.506999120532241E-5 2.2027996482128963E-4 0.0 0.3363675062821092 0.0 56-57 5.506999120532241E-5 2.2027996482128963E-4 0.0 0.3955677468278308 0.0 58-59 5.506999120532241E-5 2.2027996482128963E-4 0.0 0.46289081107633745 0.0 60-61 5.506999120532241E-5 2.2027996482128963E-4 0.0 0.5371526942167146 0.0 62-63 5.506999120532241E-5 2.2027996482128963E-4 0.0 0.6146361718426033 0.0 64-65 5.506999120532241E-5 2.2027996482128963E-4 0.0 0.6942398441298969 0.0 66-67 5.506999120532241E-5 2.2027996482128963E-4 0.0 0.7889602290030515 0.0 68-69 5.506999120532241E-5 2.2027996482128963E-4 0.0 0.9034507407189167 0.0 70-71 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.035178159682048 0.0 72-73 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.1577914951006982 0.0 74-75 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.2831858650752173 0.0 76-77 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.4208608430885232 0.0 78-79 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.4586663920509773 0.0 80-81 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 82-83 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 84-85 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 86-87 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 88-89 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 90-91 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 92-93 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 94-95 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 96-97 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 98-99 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 100-101 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 102-103 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 104-105 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 106-107 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 108-109 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 110-111 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 112-113 5.506999120532241E-5 2.2027996482128963E-4 0.0 1.459575046905865 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 265 0.0 3089.2793 108-109 CGTCGCT 20 7.561357E-7 3061.9133 110-111 CGTCGAC 10 8.718749E-4 3061.9133 110-111 CGGCGCG 60 0.0 2480.7844 108-109 CGTCGCG 55 0.0 2255.2588 108-109 CCGCGCC 220 0.0 2142.4958 108-109 CCGCGCG 70 0.0 2126.387 108-109 GTCGACG 15 0.0019615062 2041.2755 110-111 CCGTTCG 55 7.2759576E-12 1804.207 108-109 CCGCTCG 75 0.0 1653.8566 108-109 GTCCGCG 20 0.0034867434 1530.9567 110-111 CAGCGCG 65 1.6370905E-11 1526.6368 108-109 CTCCGCC 220 0.0 1391.7788 110-111 CGTCGTT 95 3.8198777E-11 1289.2267 110-111 CGCGTCG 20 0.005313074 1240.3925 108-109 CCGCGCT 60 2.309207E-8 1240.3922 108-109 CGCGAAT 25 0.005447446 1224.7654 110-111 CGCCGCT 25 0.005447446 1224.7654 110-111 TCGTTCG 45 1.6198332E-5 1102.571 108-109 CCGCACG 45 1.6198332E-5 1102.571 108-109 >>END_MODULE