FastQCFastQC Report
Fri 27 May 2016
SRR939987_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939987_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1815871
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT88030.48478113258045313No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT45940.2529915395972511No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40690.22407979421445687No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT33610.1850902404410886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28330.15601328508467838No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT28290.15579300511985708No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC27810.15314964554200158No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22950.1263856298162149No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22160.12203510051099445No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC20570.1132789719093482No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA20190.11118631224354594No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC18960.10441270332529128No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG99400.030.628221
TACATGG97900.030.300522
ACATGGG95800.028.6641353
GAGTACT69300.026.95785512-13
CATGGGG49100.026.3258084
AGTACTT70600.026.16651212-13
GTACTTT75450.024.56335814-15
AGAGTAC129150.023.79385610-11
GTCCTAC55750.021.3733391
TAGCCCT19250.021.0417374
TACTTTT88550.020.76150714-15
ATAGGAC9800.020.6659933
CATGGGT21750.020.2663674
TCCTACA62400.019.7601012
GTAGCCC12100.019.6914673
AGTGGAC38700.019.6783168
GACGTGG29450.019.5963577
ACTTTTT97000.019.4435916-17
GTAGGAC56700.019.4353393
GTATCAA225050.019.3519921
Produced by FastQC (version 0.11.5)