FastQCFastQC Report
Fri 27 May 2016
SRR939986_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939986_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967171
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT68010.34572490139393064No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38260.19449249709354194No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT25800.1311528077630262No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23290.1183933679380186No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22310.11341159461988816No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA20070.10202468417844712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG201.3913759E-62499.8071108-109
CCGCTCC5050.02079.0476108-109
CCGCGCC3600.01597.099108-109
CGCGACT200.00349657421528.9202110-111
CCGCTCG1500.01499.8842108-109
CCTCGCG1750.01428.4613108-109
CAGCGCG753.274181E-111333.2303108-109
CGACGCC753.274181E-111333.2303108-109
CGTCGCC1550.01290.223108-109
CGTCGCT752.438901E-81223.1361110-111
CGCGTTA250.0054628041223.1361110-111
CGCGTCA250.0054628041223.1361110-111
CATCGCG451.5836804E-51111.0254108-109
CGCGTGT602.0486807E-51019.28015110-111
CGACGGA250.008175672999.9229108-109
CCGTTCG755.4691554E-8999.9228108-109
CTCCGCC3700.0991.73193110-111
TTGTTCG2650.0943.3235108-109
CGTGTCG552.8906024E-5909.02075108-109
CGGCTCG703.2524356E-5873.66864110-111
Produced by FastQC (version 0.11.5)