FastQCFastQC Report
Fri 27 May 2016
SRR939986_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939986_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967171
Sequences flagged as poor quality0
Sequence length125
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69870.35518010381405585No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38530.1958650264771085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36150.1837664341330774No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26910.13679542856213311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.12164677092128748No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23530.11961339405674443No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC22140.11254740945245738No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG81000.031.9321141
TACATGG80750.031.2841722
ACATGGG79400.030.1652493
CATGGGG43300.026.969294
GAGTACT58800.025.64976312-13
AGAGTAC99600.025.14811710-11
TATACCG1200.00127812524.8250485
AGTACTT63000.024.6008412-13
TAGGACC20350.023.422164
GTACTTT66100.022.63759414-15
GTATCAA163350.021.635081
GTACACG1953.9905226E-521.3937231
GTAGGAC54350.020.609133
GTCCTAC55350.019.9194361
CTATACG905.790124E-419.8317790-91
CAACGCA176200.019.7473265
TCAACGC176900.019.6691864
AACGCAG177550.019.6027936
TACTTTT78500.019.59226614-15
ATCAACG178200.019.5256963
Produced by FastQC (version 0.11.5)