FastQCFastQC Report
Fri 27 May 2016
SRR939985_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939985_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991347
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT237711.1937146062439143No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT137100.6884787031090011No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT100690.5056376412548893No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT77360.38848076201686593No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC75800.3806468686773325No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT68650.34474152420447063No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC60440.30351314964192583No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC56580.28412928535308013No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG47940.24074156839566382No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA45890.23044702907127687No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC44660.2242703054766447No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA41510.2084518670025867No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC28860.1449270267813696No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA28360.14241616353151912No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC27600.1385996513917464No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT26750.13433118386700058No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC22680.11389275701321767No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG21550.1082182060685556No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT21200.10646060179366028No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG20970.10530560469872904No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC20790.10440169392878287No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGCT305.875336E-103106.6611110-111
CCGCTCC3600.02786.8577108-109
CCGCTCG1050.02330.4736108-109
CGTCGCT551.8189894E-122259.39110-111
CCGCGCC3300.02150.3914108-109
CGCGTCG150.00190569592071.1074110-111
CGTCGGT353.880159E-61775.2349110-111
CCGCGCG1250.01761.8379108-109
CCGTTCG1000.01712.898108-109
CGACGCC601.2732926E-111631.3314108-109
CGAGGCG150.0030725931631.3314108-109
CGACTCC702.5465852E-111398.2839108-109
CCGATCG200.0054616461223.4985108-109
CCGCACC1100.01112.2714108-109
CCTCGCG1550.01105.0955108-109
CGTCGCG700.01048.713108-109
CGCGGCT300.00762033931035.5537110-111
CGTCGCC951.1823431E-101030.3146108-109
CCGCACG755.9582817E-8978.7988108-109
CCGCGCT1051.9463187E-10932.18945108-109
Produced by FastQC (version 0.11.5)