##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR939985_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1991347
Sequences flagged as poor quality	0
Sequence length	125
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15897229362838	34.0	31.0	34.0	30.0	34.0
2	32.16841715682902	34.0	31.0	34.0	30.0	34.0
3	32.33033168001358	34.0	31.0	34.0	30.0	34.0
4	35.560563779190666	37.0	35.0	37.0	35.0	37.0
5	35.56656273366721	37.0	37.0	37.0	35.0	37.0
6	35.6447906869069	37.0	37.0	37.0	35.0	37.0
7	35.554416683782385	37.0	37.0	37.0	35.0	37.0
8	35.54398454915191	37.0	37.0	37.0	35.0	37.0
9	37.21870271730643	39.0	39.0	39.0	35.0	39.0
10-11	37.227624818778445	39.0	39.0	39.0	35.0	39.0
12-13	37.19351072414803	39.0	39.0	39.0	35.0	39.0
14-15	38.7236528842035	41.0	40.0	41.0	36.0	41.0
16-17	38.75878915126294	41.0	40.0	41.0	36.0	41.0
18-19	38.80922586570799	41.0	40.0	41.0	36.0	41.0
20-21	38.878683624702276	41.0	40.0	41.0	35.5	41.0
22-23	38.879665121146644	41.0	40.0	41.0	35.0	41.0
24-25	38.912900413639605	41.0	39.0	41.0	35.0	41.0
26-27	38.87677160233751	41.0	39.0	41.0	35.0	41.0
28-29	38.85039950345168	41.0	39.0	41.0	35.0	41.0
30-31	38.79775574021002	41.0	39.0	41.0	34.0	41.0
32-33	38.69385948305343	41.0	39.0	41.0	34.0	41.0
34-35	38.591105417589205	40.5	39.0	41.0	34.0	41.0
36-37	38.774027580326276	41.0	39.0	41.0	35.0	41.0
38-39	38.743468868057654	41.0	39.0	41.0	34.0	41.0
40-41	38.72481089433434	41.0	39.0	41.0	34.0	41.0
42-43	38.65649808898198	41.0	39.0	41.0	34.0	41.0
44-45	38.55849984959929	41.0	39.0	41.0	34.0	41.0
46-47	38.46451572729414	41.0	39.0	41.0	33.0	41.0
48-49	38.37313185497053	41.0	39.0	41.0	33.0	41.0
50-51	38.26286428231745	40.5	38.0	41.0	33.0	41.0
52-53	38.21063531368466	40.0	38.0	41.0	33.0	41.0
54-55	38.075831334267704	40.0	38.0	41.0	33.0	41.0
56-57	37.87581446126667	40.0	38.0	41.0	32.5	41.0
58-59	37.72709352011478	40.0	38.0	41.0	32.0	41.0
60-61	37.483401938486864	40.0	37.0	41.0	31.0	41.0
62-63	37.31030754559602	40.0	37.0	41.0	31.0	41.0
64-65	37.06038299703668	39.5	36.5	41.0	31.0	41.0
66-67	36.73189152869891	39.0	36.0	41.0	31.0	41.0
68-69	36.32729880829409	39.0	35.0	41.0	30.5	41.0
70-71	35.887545967628945	38.5	35.0	40.5	30.0	41.0
72-73	35.48075548862152	37.0	35.0	40.0	30.0	41.0
74-75	35.074990697251664	37.0	35.0	39.0	30.0	41.0
76-77	34.60967576218509	36.5	34.5	39.0	29.0	41.0
78-79	34.08758368079496	36.0	34.0	38.5	29.0	40.0
80-81	33.57433636628875	35.5	34.0	37.0	28.0	39.0
82-83	33.3147673409004	35.0	34.0	37.0	29.0	39.0
84-85	32.87348638886141	35.0	34.0	37.0	29.0	39.0
86-87	32.226640058211856	35.0	34.0	36.0	26.5	37.0
88-89	16.55127258082092	18.0	16.5	18.5	2.0	19.5
90-91	2.8528152049843647	2.0	2.0	2.0	2.0	2.0
92-93	2.3181482182663293	2.0	2.0	2.0	2.0	2.0
94-95	2.1069961689248533	2.0	2.0	2.0	2.0	2.0
96-97	2.0000077836760743	2.0	2.0	2.0	2.0	2.0
98-99	2.0	2.0	2.0	2.0	2.0	2.0
100-101	2.0	2.0	2.0	2.0	2.0	2.0
102-103	2.0	2.0	2.0	2.0	2.0	2.0
104-105	2.0	2.0	2.0	2.0	2.0	2.0
106-107	2.0	2.0	2.0	2.0	2.0	2.0
108-109	2.0	2.0	2.0	2.0	2.0	2.0
110-111	2.0	2.0	2.0	2.0	2.0	2.0
112-113	2.0	2.0	2.0	2.0	2.0	2.0
114-115	2.0	2.0	2.0	2.0	2.0	2.0
116-117	2.0	2.0	2.0	2.0	2.0	2.0
118-119	2.0	2.0	2.0	2.0	2.0	2.0
120-121	2.0	2.0	2.0	2.0	2.0	2.0
122-123	2.0	2.0	2.0	2.0	2.0	2.0
124-125	2.0	2.0	2.0	2.0	2.0	2.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.12218301706337797
1101	2	0.15207908368313383
1101	3	0.2313469048838641
1101	4	0.11074665906276238
1101	5	0.09348880969022844
1101	6	0.014405082718667472
1101	7	0.11386778607710824
1101	8	0.15535035899716831
1101	9	0.14111587384011415
1101	10-11	0.13627084259836408
1101	12-13	0.11984562277753241
1101	14-15	0.218487596185156
1101	16-17	0.16040058719809025
1101	18-19	0.16594446550263342
1101	20-21	0.18190068290347483
1101	22-23	0.16864899181886983
1101	24-25	0.1883664711458337
1101	26-27	0.22449798152468503
1101	28-29	0.2811486362764626
1101	30-31	0.17193552545052881
1101	32-33	0.25624895374827616
1101	34-35	0.27158402876556664
1101	36-37	0.300542210930999
1101	38-39	0.23366250771124442
1101	40-41	0.27110068124569864
1101	42-43	0.2313545878556056
1101	44-45	0.2420923859443107
1101	46-47	0.23739632337007777
1101	48-49	0.21657410016653955
1101	50-51	0.15370855781092274
1101	52-53	0.15048484859081412
1101	54-55	0.1762365772348744
1101	56-57	0.1467022503208426
1101	58-59	0.20129101882783118
1101	60-61	0.2158225715198938
1101	62-63	0.29350148715543156
1101	64-65	0.22399475656221313
1101	66-67	0.3330805776652568
1101	68-69	0.3708481037498714
1101	70-71	0.4116561135350594
1101	72-73	0.3992673391242221
1101	74-75	0.3665173973847189
1101	76-77	0.389252823510958
1101	78-79	0.41788498116417827
1101	80-81	0.42769506566099835
1101	82-83	0.45113683580260755
1101	84-85	0.5007713717845306
1101	86-87	0.5476041963715446
1101	88-89	1.0005957118982067
1101	90-91	0.0039150869442101666
1101	92-93	-0.1903287316565052
1101	94-95	-0.01031188985921494
1101	96-97	0.0
1101	98-99	0.0
1101	100-101	0.0
1101	102-103	0.0
1101	104-105	0.0
1101	106-107	0.0
1101	108-109	0.0
1101	110-111	0.0
1101	112-113	0.0
1101	114-115	0.0
1101	116-117	0.0
1101	118-119	0.0
1101	120-121	0.0
1101	122-123	0.0
1101	124-125	0.0
1102	1	-0.006967808588477453
1102	2	3.362464948395427E-4
1102	3	0.06967650628314459
1102	4	0.05788712028731169
1102	5	0.07752230045703357
1102	6	0.04081730619058277
1102	7	0.03592361910030917
1102	8	-0.053117053233847855
1102	9	0.03855574652256877
1102	10-11	0.15191035353338123
1102	12-13	0.06194314105852072
1102	14-15	0.06587980017836514
1102	16-17	0.08870564487686039
1102	18-19	-0.015233907237046651
1102	20-21	0.02244737525845153
1102	22-23	-0.0031931031634755414
1102	24-25	0.016394358185777946
1102	26-27	0.1272603666452099
1102	28-29	0.11932388844760311
1102	30-31	0.08124137201011905
1102	32-33	0.1187727966602452
1102	34-35	0.09649991460471341
1102	36-37	0.17946689303122554
1102	38-39	0.11656264197862498
1102	40-41	0.12699895331569167
1102	42-43	0.13256868298326197
1102	44-45	0.12964369575377788
1102	46-47	0.24936394018921249
1102	48-49	0.1938060126233836
1102	50-51	0.1666109744181341
1102	52-53	0.07035600947932608
1102	54-55	0.1342860059988027
1102	56-57	0.18391665798512236
1102	58-59	0.2739011588593172
1102	60-61	0.14217619437454232
1102	62-63	0.12489428332329311
1102	64-65	0.20365801205610268
1102	66-67	0.18006579865812
1102	68-69	0.16036277412310795
1102	70-71	0.170369134318058
1102	72-73	0.12543076699303413
1102	74-75	0.1594814166057219
1102	76-77	0.1311070978262734
1102	78-79	0.09785152055441415
1102	80-81	0.1377610067766284
1102	82-83	0.11430322934242554
1102	84-85	0.030619990283547338
1102	86-87	0.12522876723340204
1102	88-89	0.9770959048347443
1102	90-91	0.11295472906235826
1102	92-93	-0.026770707792688864
1102	94-95	0.11020428905155422
1102	96-97	0.0
1102	98-99	0.0
1102	100-101	0.0
1102	102-103	0.0
1102	104-105	0.0
1102	106-107	0.0
1102	108-109	0.0
1102	110-111	0.0
1102	112-113	0.0
1102	114-115	0.0
1102	116-117	0.0
1102	118-119	0.0
1102	120-121	0.0
1102	122-123	0.0
1102	124-125	0.0
1103	1	-0.05034704397868239
1103	2	0.1180326156912983
1103	3	0.10521406813649747
1103	4	0.04679544418620907
1103	5	-0.004273116157520462
1103	6	0.06081565175038861
1103	7	0.05593262127971599
1103	8	0.0371402521786095
1103	9	0.09319290127972835
1103	10-11	0.0838755634001771
1103	12-13	0.08341509729817886
1103	14-15	0.046510128571419784
1103	16-17	0.10027547017889304
1103	18-19	0.11041822792991951
1103	20-21	0.1042868836366253
1103	22-23	0.12046984074633116
1103	24-25	0.11484627105566148
1103	26-27	0.11971854374250057
1103	28-29	0.09515434216250895
1103	30-31	0.12015248661126776
1103	32-33	0.18544986685180476
1103	34-35	0.20642754150473763
1103	36-37	0.19098050466483585
1103	38-39	0.18347355836665002
1103	40-41	0.158984064419613
1103	42-43	0.21002019503103497
1103	44-45	0.13589213805571632
1103	46-47	0.10528504443878717
1103	48-49	0.05056172999971409
1103	50-51	0.13054937329567196
1103	52-53	0.16703859047529335
1103	54-55	0.13835410392940162
1103	56-57	0.2254628881992744
1103	58-59	0.1013427802320237
1103	60-61	0.14097659202630552
1103	62-63	0.06711429163279092
1103	64-65	0.07894312658346081
1103	66-67	0.11350708354805761
1103	68-69	0.08228818284776196
1103	70-71	0.19031626149988057
1103	72-73	0.2373046922562878
1103	74-75	0.309114808910131
1103	76-77	0.25021926554121876
1103	78-79	0.13612137307079308
1103	80-81	0.12916804155757688
1103	82-83	0.14861468050627025
1103	84-85	0.22301280234034238
1103	86-87	0.19513026410215417
1103	88-89	0.7674566860235714
1103	90-91	-0.6298578139387665
1103	92-93	-0.3501052679693544
1103	94-95	-0.12148507421675658
1103	96-97	0.0
1103	98-99	0.0
1103	100-101	0.0
1103	102-103	0.0
1103	104-105	0.0
1103	106-107	0.0
1103	108-109	0.0
1103	110-111	0.0
1103	112-113	0.0
1103	114-115	0.0
1103	116-117	0.0
1103	118-119	0.0
1103	120-121	0.0
1103	122-123	0.0
1103	124-125	0.0
1104	1	-0.009001485891047878
1104	2	0.06281295189877767
1104	3	0.14828281398399668
1104	4	0.0869177375586716
1104	5	0.12002109421931806
1104	6	0.08374642291128254
1104	7	0.11720559313826584
1104	8	0.03483350260645324
1104	9	0.12333506554296036
1104	10-11	0.1861172096759134
1104	12-13	0.20251880287749913
1104	14-15	0.15086107582183672
1104	16-17	0.21241831704649172
1104	18-19	0.16108231592554034
1104	20-21	0.1108371867539546
1104	22-23	0.008129672585312164
1104	24-25	0.03817058998848921
1104	26-27	0.16039594646979083
1104	28-29	0.11984127283488277
1104	30-31	0.13759976267993324
1104	32-33	0.19727087632462315
1104	34-35	0.19445204855477272
1104	36-37	0.16877186578584968
1104	38-39	0.15546240042038306
1104	40-41	0.2177805267416204
1104	42-43	0.16146356210121837
1104	44-45	0.18001394308979712
1104	46-47	0.2096146046070828
1104	48-49	0.20106428187325065
1104	50-51	0.19122204285799427
1104	52-53	0.14739404632630482
1104	54-55	0.1433950264561048
1104	56-57	0.25508934371433156
1104	58-59	0.20843044651817877
1104	60-61	0.1254101697961687
1104	62-63	0.13248416275907715
1104	64-65	0.2045909610331833
1104	66-67	0.20837623343318512
1104	68-69	0.22786088498719437
1104	70-71	0.2710462586266331
1104	72-73	0.20693961858351884
1104	74-75	0.19393728785372133
1104	76-77	0.20200183427999718
1104	78-79	0.2557006229672609
1104	80-81	0.2800667636666887
1104	82-83	0.35570560577637167
1104	84-85	0.3886714118057242
1104	86-87	0.47976368378582634
1104	88-89	0.6764537604489611
1104	90-91	-0.7414112430072994
1104	92-93	-0.3501052679693544
1104	94-95	-0.12148507421675658
1104	96-97	0.0
1104	98-99	0.0
1104	100-101	0.0
1104	102-103	0.0
1104	104-105	0.0
1104	106-107	0.0
1104	108-109	0.0
1104	110-111	0.0
1104	112-113	0.0
1104	114-115	0.0
1104	116-117	0.0
1104	118-119	0.0
1104	120-121	0.0
1104	122-123	0.0
1104	124-125	0.0
1105	1	-0.08834971542836456
1105	2	-0.08301021948465603
1105	3	-0.018788387357666636
1105	4	0.07533481507580575
1105	5	0.006824097816405583
1105	6	-0.011505825025395211
1105	7	-0.09351284295464524
1105	8	-0.09076127510676457
1105	9	-0.05634816825994449
1105	10-11	-0.10463872765586757
1105	12-13	-0.021747739157511603
1105	14-15	-0.013902613903631789
1105	16-17	-0.006195346832804205
1105	18-19	-0.0293001150914165
1105	20-21	0.027986788956127384
1105	22-23	-0.08048601234792585
1105	24-25	0.060529835241432295
1105	26-27	-0.027525936394589223
1105	28-29	0.0546829917606928
1105	30-31	0.028237546089023624
1105	32-33	-0.049019020057492924
1105	34-35	0.0538111679776776
1105	36-37	-0.040800435961394044
1105	38-39	-0.003478492010970058
1105	40-41	0.05858074750288722
1105	42-43	0.022594929635701533
1105	44-45	-0.07734468515870674
1105	46-47	0.05661225853268803
1105	48-49	0.08530307328299358
1105	50-51	-0.0168522619674647
1105	52-53	0.03342336521169642
1105	54-55	-0.03574996813175346
1105	56-57	0.056524377032573625
1105	58-59	-0.056737842659657645
1105	60-61	-0.12181458549112278
1105	62-63	-0.03041855410098293
1105	64-65	-0.0727323157754185
1105	66-67	0.02314463571231329
1105	68-69	0.054922007779829585
1105	70-71	-0.0529306921276671
1105	72-73	-0.10591704156654203
1105	74-75	-0.10258369369833531
1105	76-77	0.062313849057019866
1105	78-79	-0.03819153097097683
1105	80-81	-0.07643160491525691
1105	82-83	-0.1087769218236545
1105	84-85	-0.10508773394918336
1105	86-87	-0.11719577173446538
1105	88-89	0.5977703289131355
1105	90-91	-0.8629377994617489
1105	92-93	-0.3501052679693544
1105	94-95	-0.12148507421675658
1105	96-97	0.0
1105	98-99	0.0
1105	100-101	0.0
1105	102-103	0.0
1105	104-105	0.0
1105	106-107	0.0
1105	108-109	0.0
1105	110-111	0.0
1105	112-113	0.0
1105	114-115	0.0
1105	116-117	0.0
1105	118-119	0.0
1105	120-121	0.0
1105	122-123	0.0
1105	124-125	0.0
1106	1	-0.030858741379390153
1106	2	0.02268291325523819
1106	3	0.04617207935371681
1106	4	-0.03416278428503006
1106	5	-0.03067034340374164
1106	6	-0.016608125418088093
1106	7	-0.0873840526205214
1106	8	-0.02539520589901656
1106	9	0.033009217945235036
1106	10-11	-0.03790463330358307
1106	12-13	-0.005722436345337201
1106	14-15	-0.05759250370788749
1106	16-17	-0.06571857311094931
1106	18-19	-0.041306608500022435
1106	20-21	-0.03220184211424737
1106	22-23	-0.04251295076041828
1106	24-25	-0.09196492631517827
1106	26-27	-0.20473724728677922
1106	28-29	-0.03249634201483076
1106	30-31	0.057852181320008356
1106	32-33	0.09903319290219059
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2215	104-105	0.0
2215	106-107	0.0
2215	108-109	0.0
2215	110-111	0.0
2215	112-113	0.0
2215	114-115	0.0
2215	116-117	0.0
2215	118-119	0.0
2215	120-121	0.0
2215	122-123	0.0
2215	124-125	0.0
2216	1	0.06002010927663548
2216	2	-0.028434414998933732
2216	3	-0.030856785325660496
2216	4	0.04866801495836626
2216	5	0.09897704426112597
2216	6	0.056837094521583253
2216	7	0.04319004818214722
2216	8	0.012598385097916776
2216	9	-0.03633444507243411
2216	10-11	-0.006272721125668568
2216	12-13	-0.058967158913560525
2216	14-15	-0.11474033257754712
2216	16-17	-0.1040314425970692
2216	18-19	-0.04091162952040861
2216	20-21	-0.0634550687675528
2216	22-23	-0.14714430127180833
2216	24-25	-0.183730854906635
2216	26-27	-0.20537049516185846
2216	28-29	-0.18830278016304902
2216	30-31	-0.24446335663130014
2216	32-33	-0.3205647395499085
2216	34-35	-0.30872480812573855
2216	36-37	-0.34904066884781315
2216	38-39	-0.39206220493760213
2216	40-41	-0.4606650777121786
2216	42-43	-0.4494547333269594
2216	44-45	-0.4837701173167872
2216	46-47	-0.4788403286329128
2216	48-49	-0.5174125016080922
2216	50-51	-0.5827702698143682
2216	52-53	-0.5377084900802132
2216	54-55	-0.5357482073229818
2216	56-57	-0.5782489457136677
2216	58-59	-0.6242692147115108
2216	60-61	-0.7017515040535329
2216	62-63	-0.7266450296958098
2216	64-65	-0.7484529684683068
2216	66-67	-0.7766008996180318
2216	68-69	-0.7821567511576646
2216	70-71	-0.782602710911604
2216	72-73	-0.7892981391546385
2216	74-75	-0.8039103982353666
2216	76-77	-0.8083291686504168
2216	78-79	-0.7748248608939008
2216	80-81	-0.8159779612643945
2216	82-83	-0.7731080146668319
2216	84-85	-0.8905854534238031
2216	86-87	-1.028913824361517
2216	88-89	-1.491740941004391
2216	90-91	-0.8629377994617489
2216	92-93	-0.3501052679693544
2216	94-95	-0.12148507421675658
2216	96-97	0.0
2216	98-99	0.0
2216	100-101	0.0
2216	102-103	0.0
2216	104-105	0.0
2216	106-107	0.0
2216	108-109	0.0
2216	110-111	0.0
2216	112-113	0.0
2216	114-115	0.0
2216	116-117	0.0
2216	118-119	0.0
2216	120-121	0.0
2216	122-123	0.0
2216	124-125	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	12141.0
3	2130.0
4	1552.0
5	1309.0
6	1485.0
7	1996.0
8	2312.0
9	2600.0
10	3311.0
11	4080.0
12	5047.0
13	6140.0
14	7340.0
15	8443.0
16	9524.0
17	11218.0
18	14698.0
19	17849.0
20	17673.0
21	25703.0
22	29944.0
23	36391.0
24	57623.0
25	101202.0
26	186970.0
27	520203.0
28	867235.0
29	33921.0
30	1307.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.583912798723674	21.039025343147124	15.298689781338965	22.078372076790235
2	12.820897823411222	22.37693758985842	42.737229997885834	22.064934588844523
3	19.159687507849984	27.74432917179532	30.194830314752945	22.90115300560175
4	10.95978439669561	18.436313320156238	42.32405610750421	28.27984617564394
5	11.65231468153063	40.56027255959729	35.92543298379102	11.861979775081055
6	27.26747272072622	39.20250965803549	20.358029012522678	13.171988608715607
7	25.751664576791487	33.31368164363117	24.252277478510777	16.682376301066565
8	23.222321373422112	36.22783974867263	22.323834068095614	18.22600480980964
9	24.808446146475802	18.85528682609652	23.42239871925823	32.91386830816945
10-11	23.13721699262053	27.480760394408776	30.239914429264793	19.142108183705904
12-13	25.763561232665623	25.39802306069295	29.257817904339884	19.58059780230155
14-15	20.942156238967893	28.557629584396892	28.219968694557	22.28024548207821
16-17	19.61656472526038	30.485175811262767	29.829486677312765	20.06877278616409
18-19	19.963429427759937	30.600634736971354	29.88074889822488	19.555186937043825
20-21	22.03307523670993	28.754208602032282	28.246025163855826	20.96669099740196
22-23	20.5876947181295	29.828632980806898	28.68297226992879	20.900700031134814
24-25	21.744000417807644	29.145096258964408	29.114689705008722	19.996213618219226
26-27	20.461501687049015	28.93041745110219	30.311291803989963	20.296789057858827
28-29	19.690447470680343	30.91618348778653	28.535634044852525	20.857734996680605
30-31	20.745128799752127	29.04476216350038	28.864557508058613	21.345551528688873
32-33	20.366605886243352	29.738701093394788	29.459840979932416	20.434852040429444
34-35	21.231714319933772	29.53473943384539	29.24373031011931	19.989815936101525
36-37	20.428488964627704	28.95693943771212	30.12670070226352	20.487870895396657
38-39	20.712437360239075	30.085238785605924	29.23611505177149	19.966208802383512
40-41	21.297920452839207	29.47690683743215	29.340064790315303	19.885107919413343
42-43	19.8707206729917	30.38470944541559	28.542338427205305	21.20223145438741
44-45	20.353735436365437	29.47685662016715	28.683750245436883	21.48565769803053
46-47	21.685196000496145	29.390734010697283	28.680511231844573	20.243558756961995
48-49	20.829785690127604	29.254717476198884	29.11290381302491	20.8025930206486
50-51	19.3261443952622	30.086351119707196	30.22068233730292	20.36682214772768
52-53	20.13318120849857	30.60571562866743	28.649175658486442	20.61192750434756
54-55	19.786819851450332	29.079824166928425	29.56201676224395	21.571339219377293
56-57	21.034706658357383	28.896897426716688	28.405822792310932	21.662573122614994
58-59	22.10056810791891	29.001474881072966	28.00689683917469	20.891060171833438
60-61	20.64830991283789	29.030851981096212	30.222256593150266	20.09858151291563
62-63	20.171697850751276	29.135655413144974	30.002154320668375	20.690492415435383
64-65	20.16292501728103	30.51549818954069	28.614446865197422	20.707129927980855
66-67	20.914260548262057	29.582513745720863	27.78021610497819	21.723009601038896
68-69	20.744896145204883	29.069424678281635	29.078137413136297	21.107541763377185
70-71	22.097881484241572	28.81745371349142	28.52308010607895	20.561584696188056
72-73	21.219606628076374	28.52827759300614	29.64300044140976	20.609115337507728
74-75	20.061847583570316	30.77818682529966	28.908497614931	20.251467976199024
76-77	20.641455256165802	28.778761310811223	29.114237749623744	21.465545683399228
78-79	20.75164518739531	28.868145722085924	29.218889706596844	21.16131938392192
80-81	21.62232398471989	29.046745745467767	28.748078561898048	20.58285170791429
82-83	20.848220764683756	28.880779859521233	30.093685988229996	20.177313387565015
84-85	20.635876756664466	29.337558283471726	29.619779596202566	20.406785363661246
86-87	20.024571628582233	30.227924076530105	28.724374422807774	21.023129872079892
88-89	30.067674996815423	18.983258621640115	34.952894726433115	15.996171655111349
90-91	31.11809506955977	15.869292967792873	17.30084941874711	35.71176254390025
92-93	22.965828638787002	18.00408826237911	35.634856496437386	23.3952266023965
94-95	16.47153945519081	7.232742605436261	28.870773779379988	47.42494415999294
96-97	28.53082229342206	7.469690190113922	16.134544399224673	47.86494311723934
98-99	57.6841559824831	14.195240785453917	18.731355904758136	9.389247327304851
100-101	31.807704631133138	6.981613466100524	13.014266244216193	48.196415658550144
102-103	23.953656089133762	2.0867804272249093	37.447000513756215	36.512562969885124
104-105	42.314188514706	4.452228460082574	41.52218877025419	11.711394254957236
106-107	6.043387437242485	1.9591050208757093	7.237187035923788	84.76032050595802
108-109	19.14494489491689	11.699425252690627	22.327855856476894	46.82777399591559
110-111	41.097195831411604	2.789606086383277	26.22483843048406	29.888359651721057
112-113	27.782292603565605	2.7965208375033774	39.84234750965403	29.578839049276983
114-115	29.458782852934107	4.005892252679945	24.603207919431842	41.932116974954106
116-117	20.589927971296426	4.396508571205904	21.266377312906716	53.747186144590955
118-119	21.611135558119294	4.483069733590573	16.92034987653031	56.98544483175982
120-121	26.61059516057015	4.393945403206925	31.321957828666324	37.6735016075566
122-123	35.057420416541326	1.6995568952900997	35.55475523070694	27.688267457461635
124-125	39.20515472484738	2.0699398161544216	20.81634165120603	37.90856380779216
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	5.0
1	8.5
2	24.0
3	51.5
4	63.5
5	62.0
6	54.5
7	54.0
8	58.0
9	63.0
10	73.0
11	68.5
12	74.5
13	102.0
14	138.5
15	172.5
16	219.5
17	340.5
18	528.5
19	826.5
20	1325.0
21	2095.5
22	3291.0
23	4902.5
24	7216.0
25	10457.0
26	14258.0
27	19046.0
28	24927.5
29	32201.0
30	40695.5
31	50121.5
32	60359.5
33	70761.0
34	79930.0
35	86841.5
36	90933.0
37	91730.0
38	90667.0
39	88675.5
40	86074.5
41	83647.0
42	82916.5
43	87288.5
44	93449.0
45	91993.0
46	82905.0
47	74508.0
48	69893.5
49	66576.0
50	61875.0
51	53682.5
52	42995.0
53	32875.5
54	25292.5
55	19911.0
56	15699.5
57	12220.0
58	9444.0
59	7112.5
60	5175.0
61	3669.0
62	2599.5
63	1875.0
64	1315.5
65	888.5
66	593.0
67	397.0
68	257.0
69	193.5
70	158.5
71	101.0
72	64.5
73	55.5
74	50.5
75	33.0
76	17.5
77	14.0
78	14.5
79	11.0
80	7.5
81	8.0
82	4.5
83	4.0
84	6.0
85	6.0
86	5.0
87	2.0
88	1.5
89	4.5
90	4.5
91	2.0
92	1.5
93	3.0
94	4.0
95	5.5
96	4.5
97	5.5
98	5.5
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	fail
#Base	N-Count
1	0.0
2	0.0010043452999401912
3	0.04504488670231758
4	0.0514726966219348
5	0.051723782946919844
6	0.0
7	0.0
8	0.0
9	5.021726499700956E-5
10-11	6.026071799641148E-4
12-13	0.001832930172390849
14-15	0.0
16-17	3.5152085497906695E-4
18-19	0.006452918552115729
20-21	0.0019333647023848683
22-23	3.5152085497906695E-4
24-25	0.0
26-27	0.0
28-29	0.0010545625649372007
30-31	0.0
32-33	0.0013056488899222485
34-35	2.510863249850478E-5
36-37	2.510863249850478E-5
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	7.532589749551435E-5
50-51	2.510863249850478E-5
52-53	0.0
54-55	0.0013307575224207533
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	7.532589749551435E-5
66-67	0.0
68-69	4.5195538497308605E-4
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	4.268467524745813E-4
80-81	0.0
82-83	7.030417099581339E-4
84-85	1.0043452999401912E-4
86-87	0.0013056488899222485
88-89	19.38152918602333
90-91	26.218785575793675
92-93	40.31555022806171
94-95	44.81632282068369
96-97	48.26230687067598
98-99	93.02223570276803
100-101	51.492557550241116
102-103	53.03337389214436
104-105	74.84697543923787
106-107	81.70590057885441
108-109	12.58354269748065
110-111	39.13582113011947
112-113	51.95591225436853
114-115	86.84111809744861
116-117	66.49333340698533
118-119	71.64163252311124
120-121	80.37456555788619
122-123	75.87760947740398
124-125	49.844351587141766
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1991347.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.85099800749929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42825903482978	48.508660932948956
2	13.348319583881226	16.51213775972203
3	4.035727790640779	7.488413746131971
4	1.7540997225094959	4.339712737675596
5	0.860514406161846	2.661183741047039
6	0.4645160200692927	1.7238467659054395
7	0.2678143620933636	1.1595209903351429
8	0.174459461032938	0.8632393421390119
9	0.11928270026575186	0.6639978650819538
>10	0.4894259763122481	5.2418533598759565
>50	0.035269413267997006	1.5054220849072253
>100	0.0186620042495787	2.1216463694254983
>500	9.732065828441061E-4	0.3742549416538173
>1k	0.002027513714258539	2.751308041116485
>5k	4.055027428517078E-4	1.6996220597850435
>10k+	2.4330164571102465E-4	2.3851792622488808
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	23771	1.1937146062439143	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	13710	0.6884787031090011	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	10069	0.5056376412548893	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	7736	0.38848076201686593	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	7580	0.3806468686773325	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	6865	0.34474152420447063	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	6044	0.30351314964192583	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	5658	0.28412928535308013	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	4794	0.24074156839566382	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	4589	0.23044702907127687	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	4466	0.2242703054766447	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	4151	0.2084518670025867	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	2886	0.1449270267813696	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	2836	0.14241616353151912	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	2760	0.1385996513917464	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	2675	0.13433118386700058	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	2268	0.11389275701321767	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	2155	0.1082182060685556	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	2120	0.10646060179366028	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	2097	0.10530560469872904	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	2079	0.10440169392878287	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.0043452999401912E-4	0.0
8	0.0	0.0	0.0	1.5065179499102867E-4	0.0
9	0.0	0.0	0.0	3.0130358998205735E-4	0.0
10-11	0.0	0.0	0.0	5.021726499700956E-4	0.0
12-13	0.0	0.0	0.0	5.021726499700956E-4	0.0
14-15	0.0	0.0	0.0	5.523899149671051E-4	0.0
16-17	0.0	0.0	0.0	6.779330774596291E-4	0.0
18-19	0.0	0.0	0.0	7.030417099581339E-4	0.0
20-21	0.0	0.0	0.0	7.281503424566387E-4	0.0
22-23	0.0	0.0	0.0	9.541280349431816E-4	0.0
24-25	0.0	0.0	0.0	0.001280540257423744	0.0
26-27	0.0	0.0	0.0	0.0016320611124028108	0.0
28-29	0.0	0.0	0.0	0.003013035899820574	0.0
30-31	0.0	0.0	0.0	0.006955091202085824	0.0
32-33	0.0	0.0	0.0	0.01559246078157147	0.0
34-35	0.0	0.0	0.0	0.026690476345910583	0.0
36-37	0.0	0.0	0.0	0.04288554430744616	0.0
38-39	0.0	0.0	0.0	0.07085656091078049	0.0
40-41	0.0	0.0	0.0	0.1061341895711797	0.0
42-43	0.0	0.0	0.0	0.1406836678891223	0.0
44-45	0.0	0.0	0.0	0.18151030433169107	0.0
46-47	0.0	0.0	0.0	0.2309743103537455	0.0
48-49	0.0	0.0	0.0	0.28001146962332535	0.0
50-51	0.0	0.0	0.0	0.3350747006925463	0.0
52-53	0.0	0.0	0.0	0.3894599986843077	0.0
54-55	0.0	0.0	0.0	0.4534367942904979	0.0
56-57	0.0	0.0	0.0	0.530997360078379	0.0
58-59	0.0	0.0	0.0	0.6086332517637559	0.0
60-61	0.0	0.0	0.0	0.6969152036284987	0.0
62-63	0.0	0.0	0.0	0.7927297452427929	0.0
64-65	0.0	0.0	0.0	0.8913815623294182	0.0
66-67	0.0	0.0	0.0	1.0105220235348233	0.0
68-69	0.0	0.0	0.0	1.1468116807367075	0.0
70-71	0.0	0.0	0.0	1.2927681614505158	0.0
72-73	0.0	0.0	0.0	1.4372683414794105	0.0
74-75	0.0	0.0	0.0	1.5882967659579168	0.0
76-77	0.0	0.0	0.0	1.746682019758485	0.0
78-79	0.0	0.0	0.0	1.7893164777409463	0.0
80-81	0.0	5.021726499700956E-5	0.0	1.7904714748358774	0.0
82-83	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
84-85	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
86-87	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
88-89	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
90-91	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
92-93	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
94-95	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
96-97	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
98-99	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
100-101	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
102-103	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
104-105	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
106-107	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
108-109	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
110-111	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
112-113	0.0	5.021726499700956E-5	0.0	1.790496583468376	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCGCT	30	5.875336E-10	3106.6611	110-111
CCGCTCC	360	0.0	2786.8577	108-109
CCGCTCG	105	0.0	2330.4736	108-109
CGTCGCT	55	1.8189894E-12	2259.39	110-111
CCGCGCC	330	0.0	2150.3914	108-109
CGCGTCG	15	0.0019056959	2071.1074	110-111
CGTCGGT	35	3.880159E-6	1775.2349	110-111
CCGCGCG	125	0.0	1761.8379	108-109
CCGTTCG	100	0.0	1712.898	108-109
CGACGCC	60	1.2732926E-11	1631.3314	108-109
CGAGGCG	15	0.003072593	1631.3314	108-109
CGACTCC	70	2.5465852E-11	1398.2839	108-109
CCGATCG	20	0.005461646	1223.4985	108-109
CCGCACC	110	0.0	1112.2714	108-109
CCTCGCG	155	0.0	1105.0955	108-109
CGTCGCG	70	0.0	1048.713	108-109
CGCGGCT	30	0.0076203393	1035.5537	110-111
CGTCGCC	95	1.1823431E-10	1030.3146	108-109
CCGCACG	75	5.9582817E-8	978.7988	108-109
CCGCGCT	105	1.9463187E-10	932.18945	108-109
>>END_MODULE