##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939985_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1991347 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.85223519557365 34.0 31.0 34.0 31.0 34.0 2 32.93002826729847 34.0 33.0 34.0 31.0 34.0 3 32.97679008229103 34.0 33.0 34.0 31.0 34.0 4 36.35900925353542 37.0 37.0 37.0 35.0 37.0 5 36.26850970724841 37.0 37.0 37.0 35.0 37.0 6 36.41181823157893 37.0 37.0 37.0 35.0 37.0 7 36.40778628737232 37.0 37.0 37.0 35.0 37.0 8 36.43900535667566 37.0 37.0 37.0 35.0 37.0 9 38.28816625128619 39.0 39.0 39.0 37.0 39.0 10-11 38.2787028076975 39.0 39.0 39.0 37.0 39.0 12-13 38.26552077563579 39.0 39.0 39.0 37.0 39.0 14-15 39.76293559083374 41.0 40.0 41.0 37.0 41.0 16-17 39.80995401605044 41.0 40.0 41.0 37.5 41.0 18-19 39.87392076820363 41.0 40.0 41.0 38.0 41.0 20-21 39.882240262495685 41.0 40.0 41.0 38.0 41.0 22-23 39.8224922627749 41.0 40.0 41.0 37.5 41.0 24-25 39.77450012478991 41.0 40.0 41.0 37.0 41.0 26-27 39.667072087386074 41.0 40.0 41.0 37.0 41.0 28-29 39.607852624379376 41.0 40.0 41.0 37.0 41.0 30-31 39.50103849304014 41.0 39.5 41.0 36.5 41.0 32-33 39.33867502750651 41.0 39.0 41.0 35.5 41.0 34-35 39.20804510715611 41.0 39.0 41.0 35.0 41.0 36-37 39.19414521929126 41.0 39.0 41.0 35.0 41.0 38-39 39.25001393529104 41.0 39.5 41.0 35.0 41.0 40-41 39.214917842043604 41.0 40.0 41.0 35.0 41.0 42-43 39.108566462801306 41.0 40.0 41.0 35.0 41.0 44-45 38.937984690764594 41.0 40.0 41.0 35.0 41.0 46-47 38.69594450389611 41.0 39.0 41.0 35.0 41.0 48-49 38.43594084807921 41.0 39.0 41.0 35.0 41.0 50-51 38.27193352037591 41.0 39.0 41.0 35.0 41.0 52-53 38.21973066472091 41.0 39.0 41.0 35.0 41.0 54-55 38.13080367208728 41.0 39.0 41.0 34.5 41.0 56-57 37.99941471777646 41.0 39.0 41.0 34.0 41.0 58-59 37.86325411894562 41.0 38.5 41.0 34.0 41.0 60-61 37.65972982107086 40.0 38.0 41.0 34.0 41.0 62-63 37.51065786123664 40.0 37.5 41.0 34.0 41.0 64-65 37.29670368850833 40.0 37.0 41.0 33.0 41.0 66-67 37.01693627479289 39.0 37.0 41.0 33.0 41.0 68-69 36.66997012574905 39.0 36.0 41.0 32.5 41.0 70-71 36.27774089598648 39.0 35.5 41.0 32.0 41.0 72-73 35.909749280261046 37.5 35.0 40.0 31.5 41.0 74-75 35.55199169205568 37.0 35.0 39.0 31.0 41.0 76-77 35.15003311828627 36.5 35.0 39.0 31.0 41.0 78-79 34.745539325893475 36.0 35.0 39.0 31.0 40.5 80-81 34.413313952816864 36.0 35.0 37.0 31.0 39.0 82-83 34.11679054428987 35.0 35.0 37.0 31.0 39.0 84-85 33.81362414486274 35.0 35.0 37.0 31.0 39.0 86-87 33.60419530096965 35.0 35.0 36.0 31.0 37.0 88-89 33.368205290188 35.0 35.0 36.0 31.0 37.0 90-91 33.16577246456795 35.0 34.5 36.0 30.0 37.0 92-93 33.00942954693481 35.0 34.0 35.0 30.0 36.0 94-95 32.88537331765885 35.0 34.0 35.0 30.0 36.0 96-97 32.78651962716694 35.0 34.0 35.0 30.0 36.0 98-99 32.72321850486128 35.0 34.0 35.0 30.0 36.0 100-101 32.67489895030851 35.0 34.0 35.0 30.0 36.0 102-103 31.807703780405923 34.5 32.5 35.0 27.5 35.0 104-105 32.46666176211379 35.0 34.0 35.0 29.0 35.0 106-107 32.50307229227252 35.0 34.0 35.0 29.0 35.0 108-109 32.53682658019923 35.0 34.0 35.0 29.5 35.0 110-111 32.53629980108941 35.0 34.0 35.0 29.5 35.0 112-113 32.446178892980484 35.0 34.0 35.0 29.0 35.0 114-115 32.38287099134405 35.0 34.0 35.0 29.0 35.0 116-117 32.3093667251363 35.0 34.0 35.0 29.0 35.0 118-119 32.23316127224437 35.0 34.0 35.0 29.0 35.0 120-121 32.157000763804604 35.0 34.0 35.0 29.0 35.0 122-123 32.00607428037404 35.0 34.0 35.0 29.0 35.0 124-125 29.946832972857067 33.5 30.5 34.5 22.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12510368122247684 1101 2 0.14608534349875413 1101 3 0.13231249056357086 1101 4 0.06862351484953422 1101 5 0.00501007076068305 1101 6 0.04537727603698727 1101 7 0.020822370751595543 1101 8 0.07313535724028242 1101 9 0.1350503452854923 1101 10-11 0.1452854004142523 1101 12-13 0.11882996983914751 1101 14-15 0.22684187302875358 1101 16-17 0.1869467988184681 1101 18-19 0.2462651719501281 1101 20-21 0.2312892647962954 1101 22-23 0.222625748881363 1101 24-25 0.2261742421249906 1101 26-27 0.24819440367027568 1101 28-29 0.26990264794569896 1101 30-31 0.27640632073070037 1101 32-33 0.2635826690008827 1101 34-35 0.21729165567838038 1101 36-37 0.21807336907345842 1101 38-39 0.16343872740579002 1101 40-41 0.23174054741186723 1101 42-43 0.17292912114238845 1101 44-45 0.27484494079155297 1101 46-47 0.2442755117704678 1101 48-49 0.1256979992307805 1101 50-51 0.20777917959432557 1101 52-53 0.16565314479916537 1101 54-55 0.1954421065399714 1101 56-57 0.20476721165106682 1101 58-59 0.23781171911806354 1101 60-61 0.31167702102785455 1101 62-63 0.35678896407642924 1101 64-65 0.33036277177913576 1101 66-67 0.3516159024687937 1101 68-69 0.31757470359491435 1101 70-71 0.3650756261821613 1101 72-73 0.3211827485829204 1101 74-75 0.3635835043295472 1101 76-77 0.28071858562998386 1101 78-79 0.3208544613845916 1101 80-81 0.3141872983938825 1101 82-83 0.28796066003896215 1101 84-85 0.31366862775738724 1101 86-87 0.27539522878294065 1101 88-89 0.22972674500861956 1101 90-91 0.2627153270095519 1101 92-93 0.3240055742166277 1101 94-95 0.31446998347063015 1101 96-97 0.26467677388191646 1101 98-99 0.2863290885982721 1101 100-101 0.3056340929290968 1101 102-103 0.3344935177219668 1101 104-105 0.2640730229103383 1101 106-107 0.3344949121940317 1101 108-109 0.34264705775474624 1101 110-111 0.32457092323515724 1101 112-113 0.32713830760273055 1101 114-115 0.316682284675025 1101 116-117 0.3195805817425068 1101 118-119 0.3468652680107809 1101 120-121 0.3656895601937791 1101 122-123 0.30395133510894823 1101 124-125 0.4466808325101965 1102 1 0.05353512432607488 1102 2 0.12265153923578254 1102 3 0.09827492174211017 1102 4 0.04480836107260444 1102 5 -0.03060704171618056 1102 6 -0.008977914983894664 1102 7 -0.03171846291243696 1102 8 0.0026420359971837115 1102 9 0.03106543477308321 1102 10-11 0.09576575412894783 1102 12-13 0.0694116238021607 1102 14-15 0.07956824104474691 1102 16-17 0.24105716948278655 1102 18-19 0.08671913139849607 1102 20-21 0.07349815851944896 1102 22-23 0.0914776359925824 1102 24-25 0.13847893243615061 1102 26-27 0.07300220392647105 1102 28-29 0.08610547873247754 1102 30-31 0.10085449825150761 1102 32-33 0.10201254256747916 1102 34-35 0.1209961742314718 1102 36-37 0.19610067353416838 1102 38-39 0.16722316212231192 1102 40-41 0.12855911647389462 1102 42-43 0.08471511031738288 1102 44-45 0.10365992664858936 1102 46-47 0.14175143582680505 1102 48-49 0.18196082882498388 1102 50-51 0.09725382334711696 1102 52-53 0.0923177278883287 1102 54-55 0.058421183720753334 1102 56-57 0.14160595851627988 1102 58-59 0.10509866132414913 1102 60-61 0.10625540158094537 1102 62-63 0.072901118118601 1102 64-65 0.1076186727927535 1102 66-67 0.18198219402297866 1102 68-69 0.11153938832701726 1102 70-71 0.10139628875980122 1102 72-73 0.11859479453951138 1102 74-75 0.16265370786902622 1102 76-77 0.14170483059152872 1102 78-79 0.20488383982737446 1102 80-81 0.1684832719489009 1102 82-83 0.13964948870515315 1102 84-85 0.16072540318817374 1102 86-87 0.12586187475091037 1102 88-89 0.08830083777854014 1102 90-91 0.13270351778574252 1102 92-93 0.0962791205272211 1102 94-95 0.08189489414752416 1102 96-97 0.09415775061370368 1102 98-99 0.12482359419069411 1102 100-101 0.11419629422873356 1102 102-103 0.1200852365980154 1102 104-105 0.1366424509340476 1102 106-107 0.09601397727087146 1102 108-109 0.19050463974934218 1102 110-111 0.19288197119924888 1102 112-113 0.17600937185162024 1102 114-115 0.18975595216210195 1102 116-117 0.17127969121874287 1102 118-119 0.15586645989936443 1102 120-121 0.14216473649253913 1102 122-123 0.09312941176129641 1102 124-125 0.12999658565835048 1103 1 0.003256526987648556 1103 2 0.014522816329069599 1103 3 0.09558332604520103 1103 4 0.012739395693166955 1103 5 -0.06353173364995968 1103 6 -0.003768559697391538 1103 7 -0.011216725284008078 1103 8 -0.01745195272358302 1103 9 0.0923532526184232 1103 10-11 0.03959750954993524 1103 12-13 0.04665940792462209 1103 14-15 0.04384205702437072 1103 16-17 0.09883372441346694 1103 18-19 0.06667149897302238 1103 20-21 0.0839625593490112 1103 22-23 0.07714648073908847 1103 24-25 0.19083090832661753 1103 26-27 0.1729503061889872 1103 28-29 0.10968957697798487 1103 30-31 0.03540926668249256 1103 32-33 0.06922971688220514 1103 34-35 0.07090959585756451 1103 36-37 0.07804684135381024 1103 38-39 -0.04175015117125014 1103 40-41 0.06953203387403306 1103 42-43 0.014373628266660887 1103 44-45 0.0012117148227019925 1103 46-47 0.021877786037158842 1103 48-49 0.06401862473500586 1103 50-51 0.07748379586236354 1103 52-53 0.05903577278226635 1103 54-55 0.11878973491735678 1103 56-57 0.0848510004241021 1103 58-59 0.053146641688258 1103 60-61 0.13864066875105863 1103 62-63 0.12441908031717475 1103 64-65 0.11521770842195878 1103 66-67 0.13022166052062545 1103 68-69 0.1868377303834592 1103 70-71 0.22547976952080973 1103 72-73 0.21592290026809735 1103 74-75 0.14861960588704193 1103 76-77 0.1628708086407613 1103 78-79 0.11842288854557381 1103 80-81 0.038869804859004375 1103 82-83 0.03558560013929224 1103 84-85 0.034594186366057045 1103 86-87 0.0500400262563403 1103 88-89 0.0785682803004022 1103 90-91 0.0827605373610183 1103 92-93 0.09965527082605519 1103 94-95 0.15546360023809314 1103 96-97 0.11552487235798736 1103 98-99 0.18246538284567748 1103 100-101 0.1984629143259724 1103 102-103 0.12591487374944066 1103 104-105 0.10361732036048465 1103 106-107 0.1608802879798077 1103 108-109 0.17628651126754136 1103 110-111 0.21137520244749197 1103 112-113 0.19640234535620493 1103 114-115 0.2737150621798463 1103 116-117 0.2452748619051519 1103 118-119 0.18628784528389275 1103 120-121 0.21034752043050986 1103 122-123 0.17235018907910415 1103 124-125 0.2173939873880002 1104 1 0.10459959755394266 1104 2 0.14989069769288932 1104 3 0.0882805286275925 1104 4 0.056076116587540525 1104 5 -0.034845800532202986 1104 6 0.027263459947157287 1104 7 0.03316579846551804 1104 8 -0.009037918163841141 1104 9 0.011311487848061574 1104 10-11 0.054051548901398405 1104 12-13 0.06762301309413488 1104 14-15 0.08500767082281158 1104 16-17 0.12929230291850757 1104 18-19 0.08844463066181163 1104 20-21 0.19370228747082052 1104 22-23 0.1793500631008058 1104 24-25 0.18947919381462697 1104 26-27 0.20910103114105993 1104 28-29 0.18544574081779786 1104 30-31 0.1612777190686785 1104 32-33 0.1606237205299692 1104 34-35 0.22369661909272054 1104 36-37 0.286964388932752 1104 38-39 0.2774974334569862 1104 40-41 0.19277044802699095 1104 42-43 0.2575993654812905 1104 44-45 0.24755939315945596 1104 46-47 0.21160590148259928 1104 48-49 0.23332907847340323 1104 50-51 0.2160452268822155 1104 52-53 0.1264464552987974 1104 54-55 0.10442729083725766 1104 56-57 0.16586418543293036 1104 58-59 0.16212299101263739 1104 60-61 0.22754906062198899 1104 62-63 0.1500192903179567 1104 64-65 0.22389597528921001 1104 66-67 0.21383916834250272 1104 68-69 0.21575580245512782 1104 70-71 0.3027311832005779 1104 72-73 0.3001972970409881 1104 74-75 0.23985473406759183 1104 76-77 0.22158586001621927 1104 78-79 0.20045386951628785 1104 80-81 0.19216941789285613 1104 82-83 0.22166944590329507 1104 84-85 0.13289144363826466 1104 86-87 0.18144104945000095 1104 88-89 0.2348142185052211 1104 90-91 0.1966512053241516 1104 92-93 0.2232451714894168 1104 94-95 0.2069775701400971 1104 96-97 0.21020713988263395 1104 98-99 0.2682138969845127 1104 100-101 0.20478808021270822 1104 102-103 0.21985368556208584 1104 104-105 0.26401629892362877 1104 106-107 0.24530076616186136 1104 108-109 0.23375449206855592 1104 110-111 0.20049178737858853 1104 112-113 0.23902126230672138 1104 114-115 0.2634407970393511 1104 116-117 0.20301691561753188 1104 118-119 0.25997296301913764 1104 120-121 0.23501688314256342 1104 122-123 0.27796755712112997 1104 124-125 0.3668893095366563 1105 1 -7.199038675977931E-4 1105 2 -0.03678861351637863 1105 3 -0.03824922944836828 1105 4 -0.0074719407715235775 1105 5 -0.11855289928983126 1105 6 0.0022776947741860454 1105 7 -0.03846173781142426 1105 8 0.016851855691882633 1105 9 0.008947559191270216 1105 10-11 0.03433528106899786 1105 12-13 0.04669496310025778 1105 14-15 -0.014747373552928877 1105 16-17 0.02452203262197372 1105 18-19 0.005834718849726528 1105 20-21 0.06087544029553271 1105 22-23 0.04995214527220071 1105 24-25 -0.016666972110009226 1105 26-27 0.06622023008466726 1105 28-29 0.11585905606528968 1105 30-31 0.031353039765235735 1105 32-33 0.01445075134910212 1105 34-35 -0.00515361012200799 1105 36-37 -0.009596916532068178 1105 38-39 -0.038892427362355875 1105 40-41 0.024255298381547163 1105 42-43 -0.03332460839659035 1105 44-45 -0.015961809931198445 1105 46-47 -0.10344246049205452 1105 48-49 -0.1283997995695927 1105 50-51 -0.14863106197692133 1105 52-53 -0.17104770502130862 1105 54-55 -0.1728656215792057 1105 56-57 -0.11977553400980412 1105 58-59 -0.20438949336791978 1105 60-61 -0.10495415071613223 1105 62-63 -0.15693914738791648 1105 64-65 -0.0390993772583883 1105 66-67 -0.09994767459634346 1105 68-69 -0.12785506790010004 1105 70-71 -0.09874231572962344 1105 72-73 -0.11158594540969347 1105 74-75 -0.0912943468386942 1105 76-77 -0.11383725199874561 1105 78-79 -0.10510968874350368 1105 80-81 -0.1856208875583576 1105 82-83 -0.09110061500038569 1105 84-85 -0.12503305542212928 1105 86-87 -0.06724362581132937 1105 88-89 -0.0343458343919778 1105 90-91 -0.024373330562191597 1105 92-93 -0.1684205680511397 1105 94-95 -0.13409350099905737 1105 96-97 -0.1447385758716777 1105 98-99 -0.20584762467476736 1105 100-101 -0.060941585846990165 1105 102-103 -0.13953294400010208 1105 104-105 -0.11160453143804716 1105 106-107 -0.10446205149825971 1105 108-109 -0.0370390436681447 1105 110-111 -0.1149314533814092 1105 112-113 -0.13491877749248715 1105 114-115 -0.14385829270904793 1105 116-117 -0.20674314738493393 1105 118-119 -0.07972673071345326 1105 120-121 -0.23468461544949903 1105 122-123 -0.1623596107021399 1105 124-125 -0.13403892993103028 1106 1 0.054582418383354536 1106 2 0.0698212468169217 1106 3 0.002073123253389042 1106 4 0.02196886481816307 1106 5 -0.0047806924353892555 1106 6 0.07936949189944897 1106 7 0.00985395189182725 1106 8 -0.0014190999660144143 1106 9 0.06669715782852137 1106 10-11 0.1237814664269834 1106 12-13 0.12575141203862472 1106 14-15 -0.0019235510720889692 1106 16-17 0.053126194734844034 1106 18-19 0.05040176527212736 1106 20-21 0.07961747899218352 1106 22-23 0.08138398332515351 1106 24-25 0.09347648209695336 1106 26-27 0.10727299703800242 1106 28-29 0.09134389472876592 1106 30-31 0.014717919959402082 1106 32-33 0.20273416244253895 1106 34-35 0.22254296340840085 1106 36-37 0.143637680936898 1106 38-39 0.09697098305272789 1106 40-41 0.04975667205968648 1106 42-43 0.10174973792858566 1106 44-45 0.08103960520224973 1106 46-47 0.12256033397252963 1106 48-49 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2216 32-33 -0.42976274912390267 2216 34-35 -0.3242605353272978 2216 36-37 -0.25513437838738895 2216 38-39 -0.2079390587683605 2216 40-41 -0.2199213363162471 2216 42-43 -0.2230236732032509 2216 44-45 -0.2046377521677769 2216 46-47 -0.22461529629126176 2216 48-49 -0.2236535302600089 2216 50-51 -0.2216113031076361 2216 52-53 -0.23889574462948815 2216 54-55 -0.35296827858403645 2216 56-57 -0.3676344832184455 2216 58-59 -0.36806769685365026 2216 60-61 -0.4062459541698402 2216 62-63 -0.4275351422337508 2216 64-65 -0.3013488605073533 2216 66-67 -0.3383244832249659 2216 68-69 -0.45649742131924853 2216 70-71 -0.4667048264427578 2216 72-73 -0.42519961062026823 2216 74-75 -0.41849876821098064 2216 76-77 -0.4590709650736642 2216 78-79 -0.42668359971107606 2216 80-81 -0.43306873425604664 2216 82-83 -0.390224492160435 2216 84-85 -0.3466671675148518 2216 86-87 -0.2911153551553767 2216 88-89 -0.2763463874712784 2216 90-91 -0.32929026857875243 2216 92-93 -0.33821026075849403 2216 94-95 -0.2944075403166124 2216 96-97 -0.3455830041537524 2216 98-99 -0.3875475205831762 2216 100-101 -0.4114015647024587 2216 102-103 -0.34349761809849255 2216 104-105 -0.4378672908144452 2216 106-107 -0.41853918011218383 2216 108-109 -0.4257218252566801 2216 110-111 -0.41786933501354184 2216 112-113 -0.447445096949906 2216 114-115 -0.5929062326739967 2216 116-117 -0.7278504254933722 2216 118-119 -0.6265014109505813 2216 120-121 -0.7006931288672362 2216 122-123 -0.7849308066879388 2216 124-125 -0.9026655991777481 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 200.0 3 6.0 4 28.0 5 76.0 6 138.0 7 360.0 8 676.0 9 1537.0 10 3580.0 11 5342.0 12 5922.0 13 9547.0 14 17101.0 15 18668.0 16 6019.0 17 3652.0 18 3073.0 19 2855.0 20 2830.0 21 3136.0 22 3248.0 23 3348.0 24 3915.0 25 4988.0 26 6536.0 27 8382.0 28 10874.0 29 13847.0 30 17942.0 31 22712.0 32 29828.0 33 40415.0 34 58676.0 35 95065.0 36 206560.0 37 689967.0 38 683554.0 39 6744.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.81116401478624 20.808070154291226 15.691096110575378 21.689669720347155 2 13.058999762472338 22.57582430385061 42.22594053171044 22.13923540196661 3 19.026568448391966 28.071601785123335 30.434524972292625 22.46730479419207 4 10.936416405578736 18.575416539658836 42.11139495025227 28.376772104510163 5 11.330471369211978 40.15340355655279 35.93628919884281 12.579835875392423 6 26.779109818630303 39.60228930467669 19.939668977832593 13.67893189886042 7 24.882001981573275 33.802797804702045 24.346334415850176 16.968865797874503 8 23.33586260958035 35.369124517223774 22.27266267506366 19.02235019813222 9 24.8798928564434 18.18653403952199 23.65996483787105 33.273608266163556 10-11 23.0814117278405 27.147001501998396 30.198754913131665 19.57283185702944 12-13 26.130955579313902 25.11526117748439 29.59265763325026 19.161125609951455 14-15 21.10192246755588 27.80248746200436 29.409439941908666 21.68615012853109 16-17 19.035802435037624 29.937002377789884 30.757955050480955 20.269240136691533 18-19 19.289079201163837 29.43952008364188 32.093201235143844 19.178199480050438 20-21 21.22500623345435 27.524925656797677 30.815084733548385 20.43498337619958 22-23 19.783628218371437 28.60923869894709 31.2805541592324 20.326578923449073 24-25 21.043871309219337 27.952913279302905 31.63705270854351 19.366162702934243 26-27 19.80305290840823 27.71382888065214 32.88573011132666 19.597388099612974 28-29 18.975321729462518 29.63775273721757 31.188512097590227 20.198413435729684 30-31 19.9831822379525 27.81752753287097 31.387146489285893 20.812143739890637 32-33 19.713615959448553 28.495686588023084 31.94054074955294 19.850156702975426 34-35 20.62146878469699 28.272244867418888 31.68342835276825 19.42285799511587 36-37 19.891937467452937 27.71774582732191 32.49134882067264 19.898967884552516 38-39 20.145660198850326 28.829430531193207 31.60955875595765 19.415350513998817 40-41 20.731695681365427 28.34812315482937 31.525193750762675 19.39498741304253 42-43 19.510035167150676 29.245204376735952 30.599086949687827 20.64567350642555 44-45 20.118819070709424 28.26164902450452 30.489763963789336 21.12976794099672 46-47 21.507728185996715 28.52290434565146 30.136887242655348 19.832480225696475 48-49 20.929878117676125 28.530462044033513 30.043734216085895 20.495925622204467 50-51 19.420673544088498 29.429376196112482 30.97717273785031 20.172777521948714 52-53 20.204288855734333 29.979179921932243 29.385134785650113 20.43139643668331 54-55 19.83697467091371 28.460961349277646 30.276114609859555 21.425949369949084 56-57 21.021650169458162 28.192926697356114 29.27149311496188 21.513930018223846 58-59 22.08359467234992 28.287184503755498 28.933982876916982 20.6952379469776 60-61 20.580391061929436 28.3861878416971 31.103670028377778 19.92975106799568 62-63 20.10192096103793 28.53161704112844 30.860417596732265 20.506044401101363 64-65 20.0604415001504 29.93973426027709 29.476103361192195 20.523720878380313 66-67 20.750376503944317 28.94302198461644 28.701502048613325 21.605099462825915 68-69 20.627946811881607 28.431860444211882 29.92230886932313 21.017883874583383 70-71 21.902988278788175 28.140098134579254 29.43962051817187 20.517293068460695 72-73 20.969650191553757 27.775395247538476 30.635670227238144 20.619284333669622 74-75 19.809330066532855 30.07286022978416 29.825414656511395 20.29239504717159 76-77 20.397524891442828 28.035997744240454 30.019479277092344 21.546998087224374 78-79 20.488116837497433 28.14913724227872 30.175529428070547 21.1872164921533 80-81 21.319639419950416 28.288615695807913 29.7010264911138 20.69071839312787 82-83 20.54980874754626 28.16442839947031 30.993418023076842 20.292344829906593 84-85 20.36490877782727 28.631624724369985 30.447380592131857 20.556085905670884 86-87 19.690767103874915 29.480899109999413 29.61344758095902 21.214886205166653 88-89 20.079900690336743 28.797768545612595 29.153131021363933 21.969199742686733 90-91 20.57849749740249 28.00071911701269 29.86542669199646 21.555356693588358 92-93 20.82886608913464 29.147381144521773 29.983172194499502 20.04058057184408 94-95 20.33139041759381 28.933199152127443 29.82631769509631 20.909092735182444 96-97 19.54809985401841 28.772358609524108 30.790012991206456 20.88952854525103 98-99 20.438919140963286 29.01876566012504 29.471328993003898 21.07098620590778 100-101 19.7766888442848 28.175978370419617 30.042704762153456 22.004628023142125 102-103 20.507652628227596 28.07443422576333 29.782829870509435 21.63508327549964 104-105 21.473855745534028 27.89566654070253 29.198704871045784 21.43177284271766 106-107 20.27696448928552 28.01853628159479 30.442540540269082 21.26195868885061 108-109 19.602720973652996 28.68911279104686 29.87921235184644 21.828953883453703 110-111 19.392609488684528 29.58517136922626 29.476672010027844 21.545547132061373 112-113 20.024447679805704 28.018306631558698 28.990915487263653 22.966330201371942 114-115 19.54912365590453 28.408735092006022 28.877760789612385 23.164380462477066 116-117 20.801162445595107 28.14626888007396 28.3034762386137 22.74909243571723 118-119 20.155909777517095 27.258951743144326 30.236814935038847 22.348323544299728 120-121 19.27654636056077 28.980102313169493 29.541408611400282 22.201942714869457 122-123 19.870558742708383 28.13690160536084 29.017595293191278 22.974944358739492 124-125 20.055274851398934 28.046977848629666 29.0207533037734 22.876993996198003 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 35.0 1 24.0 2 27.5 3 44.5 4 34.5 5 31.5 6 65.0 7 152.5 8 299.0 9 372.0 10 389.0 11 449.5 12 565.0 13 744.5 14 947.0 15 1148.0 16 1397.0 17 1718.0 18 2137.0 19 2591.5 20 3176.0 21 3866.0 22 4740.5 23 5995.5 24 7628.0 25 9870.5 26 12870.0 27 16452.0 28 20402.0 29 25337.5 30 32207.5 31 39889.5 32 49070.0 33 60489.5 34 72936.5 35 84653.5 36 92708.0 37 95283.5 38 93028.5 39 87868.0 40 81442.5 41 77006.5 42 73917.0 43 70290.5 44 67888.5 45 67068.0 46 66752.5 47 83878.0 48 120910.0 49 111849.5 50 71509.5 51 53533.5 52 43297.5 53 36521.0 54 30675.0 55 25421.0 56 20546.0 57 15930.0 58 11984.0 59 8835.0 60 6379.0 61 4457.5 62 2995.5 63 1962.0 64 1345.0 65 996.0 66 719.0 67 489.0 68 330.5 69 205.0 70 170.5 71 130.0 72 82.0 73 66.5 74 56.0 75 36.5 76 15.5 77 5.0 78 3.0 79 2.5 80 0.5 81 0.0 82 0.0 83 0.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 1.5 98 5.0 99 6.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015416700354081936 2 0.0 3 0.0 4 0.0 5 0.13739443703181817 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.0043452999401912E-4 18-19 0.0 20-21 0.003791403507274222 22-23 3.013035899820574E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 8.03476239952153E-4 92-93 0.0 94-95 5.021726499700956E-4 96-97 0.0 98-99 0.0011801057274297248 100-101 0.0 102-103 2.510863249850478E-4 104-105 0.0019835819673818776 106-107 0.005875420004650119 108-109 5.021726499700956E-5 110-111 0.00456977111472787 112-113 0.007833893339533491 114-115 0.004594879747226375 116-117 0.0014060834199162678 118-119 1.506517949910287E-4 120-121 5.021726499700956E-4 122-123 9.541280349431816E-4 124-125 0.001280540257423744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1991347.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.950744866900806 #Duplication Level Percentage of deduplicated Percentage of total 1 77.61674822508702 46.53181870240669 2 13.916395603111944 16.685965645380488 3 4.21951727489545 7.588896108262132 4 1.7424250567715929 4.178387201128357 5 0.8467627287151639 2.5382028156001764 6 0.44946755396218585 1.6167548792122184 7 0.29967037858930906 1.2575823687682668 8 0.18130120108859998 0.8695313640420268 9 0.13011072514922764 0.7020211399081919 >10 0.5311936346423339 5.641093783299509 >50 0.037943110101298534 1.5748445016041717 >100 0.024028841450671544 2.7436820214032367 >500 0.0013390691124770585 0.5769994958090517 >1k 0.0024270627663646625 3.3697774032632575 >5k 5.021509171788957E-4 2.208804243826247 >10k+ 1.673836390596319E-4 1.9156383260860486 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24542 1.2324321175566086 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 13638 0.6848630600292164 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9708 0.48750920859096886 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7536 0.37843730901746403 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7513 0.3772823119225328 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7093 0.3561910606237888 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6153 0.30898683152659984 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6020 0.30230793528199756 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4932 0.24767155096525115 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4816 0.24184634822559803 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4670 0.23451462753603466 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4482 0.22507378171659687 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4377 0.21980096889191086 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3389 0.1701863110748654 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2891 0.14517811310635464 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2800 0.14060834199162678 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2774 0.13930269310170454 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2676 0.1343814011319976 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2281 0.11454558145817882 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2258 0.11339058436324759 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2230 0.11198450094333133 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2209 0.11092993837839413 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2156 0.10826842333355262 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.021726499700956E-5 0.0 2 0.0 0.0 0.0 5.021726499700956E-5 0.0 3 0.0 0.0 0.0 1.0043452999401912E-4 0.0 4 0.0 0.0 0.0 1.0043452999401912E-4 0.0 5 0.0 0.0 0.0 1.0043452999401912E-4 0.0 6 0.0 0.0 0.0 1.5065179499102867E-4 0.0 7 0.0 0.0 0.0 1.5065179499102867E-4 0.0 8 0.0 0.0 0.0 2.0086905998803825E-4 0.0 9 0.0 0.0 0.0 3.0130358998205735E-4 0.0 10-11 0.0 0.0 0.0 4.5195538497308605E-4 0.0 12-13 0.0 0.0 0.0 4.5195538497308605E-4 0.0 14-15 0.0 0.0 0.0 4.5195538497308605E-4 0.0 16-17 0.0 0.0 0.0 5.272812824686004E-4 0.0 18-19 0.0 0.0 0.0 5.523899149671051E-4 0.0 20-21 0.0 0.0 0.0 5.523899149671051E-4 0.0 22-23 0.0 0.0 0.0 7.281503424566387E-4 0.0 24-25 0.0 0.0 0.0 0.001029453932438696 0.0 26-27 0.0 0.0 0.0 0.001380974787417763 0.0 28-29 0.0 0.0 0.0 0.002736840942337021 0.0 30-31 0.0 0.0 0.0 0.0064780271846142335 0.0 32-33 0.0 0.0 0.0 0.014939636336610346 0.0 34-35 0.0 0.0 0.0 0.025786565575964412 0.0 36-37 0.0 0.0 0.0 0.04198163353749999 0.0 38-39 0.0 0.0 0.0 0.06997775877333283 0.0 40-41 0.0 0.0 0.0 0.10492897521125148 0.0 42-43 0.0 0.0 0.0 0.13942823626419704 0.0 44-45 0.0 0.0 0.0 0.1801293295442733 0.0 46-47 0.0 0.0 0.0 0.22981931325881427 0.0 48-49 0.0 0.0 0.0 0.2788313638958956 0.0 50-51 0.0 0.0 0.0 0.33384437770011954 0.0 52-53 0.0 0.0 0.0 0.3883552188543734 0.0 54-55 0.0 0.0 0.0 0.45248266625555467 0.0 56-57 0.0 0.0 0.0 0.5301185579409314 0.0 58-59 0.0 0.0 0.0 0.6078799927888008 0.0 60-61 0.0 0.0 0.0 0.6964381396110271 0.0 62-63 0.0 0.0 0.0 0.7922024639603243 0.0 64-65 0.0 0.0 0.0 0.8907036292519586 0.0 66-67 0.0 0.0 0.0 1.0094925696023846 0.0 68-69 0.0 0.0 0.0 1.1465354857792238 0.0 70-71 0.0 0.0 0.0 1.2931196823054947 0.0 72-73 0.0 0.0 0.0 1.4377454054968823 0.0 74-75 0.0 0.0 0.0 1.589050024932872 0.0 76-77 0.0 0.0 0.0 1.7472846269384492 0.0 78-79 0.0 0.0 0.0 1.9453164114541566 0.0 80-81 0.0 0.0 0.0 2.1659710738510163 0.0 82-83 0.0 0.0 0.0 2.3825581377831186 0.0 84-85 0.0 0.0 0.0 2.6054976857373426 0.0 86-87 0.0 0.0 0.0 2.8349152608761807 0.0 88-89 0.0 0.0 0.0 3.095768843903147 0.0 90-91 0.0 0.0 0.0 3.3848445298584324 0.0 92-93 0.0 0.0 0.0 3.70646100353178 0.0 94-95 0.0 0.0 0.0 4.011455562491118 0.0 96-97 0.0 0.0 0.0 4.323957602567509 0.0 98-99 0.0 0.0 0.0 4.660363060782475 0.0 100-101 0.0 0.0 0.0 5.040432430912342 0.0 102-103 0.0 0.0 0.0 5.475991878863905 0.0 104-105 0.0 0.0 0.0 5.886794215171942 0.0 106-107 0.0 0.0 0.0 6.301613932679739 0.0 108-109 0.0 0.0 0.0 6.72426754352707 0.0 110-111 0.0 0.0 0.0 7.1838810614122 0.0 112-113 0.0 0.0 0.0 7.701319759941386 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11010 0.0 29.931921 1 TACATGG 11150 0.0 28.964535 2 ACATGGG 10790 0.0 27.556324 3 CATGGGG 4785 0.0 27.395733 4 AGTACTT 7725 0.0 26.069736 12-13 CAGTCGA 115 0.0010056193 25.867151 9 GAGTACT 7415 0.0 25.59505 12-13 GTACTTT 8140 0.0 23.206633 14-15 TAGGACC 1960 0.0 22.800657 4 AGAGTAC 15070 0.0 22.70027 10-11 TACTTTT 9555 0.0 21.264385 14-15 ATACCGA 145 0.0038704462 20.515327 6 GTAGGAC 4970 0.0 20.261541 3 CATGGGT 2205 0.0 19.997019 4 GTGTAGC 1530 0.0 19.864403 1 TAGCCCT 1900 0.0 19.757368 4 ATGGGGA 2975 0.0 19.427948 5 GTCCTAC 4670 0.0 19.141287 1 TAGGACG 5190 0.0 19.058245 4 CATGGGC 2590 0.0 18.864975 4 >>END_MODULE