FastQCFastQC Report
Fri 27 May 2016
SRR939984_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939984_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720919
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18350.25453622390310143No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA14720.20418382647703834No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA14520.2014095897042525No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC13580.188370676872159No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT11980.16617678268987224No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA10700.14842166734404283No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA8960.12428580742080596No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA8200.11374370768421974No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA8030.11138560642735176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACG52.2028826E-46082.7983110-111
CTCGTCG108.810574E-43041.3992110-111
CGCGGCT108.810574E-43041.3992110-111
CCGCTCC1450.02524.4814108-109
CCGCGCC1200.02033.61108-109
CGGCGCT150.0019821642027.5994110-111
CCACGCG404.80577E-91830.249108-109
CCGTTCG150.0030816131626.8881108-109
CCGCGCT357.952389E-61394.4755108-109
CTCCGCC902.910383E-111351.733110-111
CGCGGCC200.0054776811220.166108-109
CCGCTCT401.186882E-51220.166108-109
CACCGCG200.0054776811220.166108-109
CGCGTCT250.0055048161216.5597110-111
CCGATCT300.0079260761013.7997110-111
CGCGTAT300.0079260761013.7997110-111
CGGCACG250.008557715976.1328108-109
ACGCTCG250.008557715976.1328108-109
CCGCACA250.008557715976.1328108-109
CCGCGCG755.932816E-8976.13275108-109
Produced by FastQC (version 0.11.5)