##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939984_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 720919 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.692993248894812 34.0 31.0 34.0 30.0 34.0 2 31.851180229679063 34.0 31.0 34.0 30.0 34.0 3 32.0030683058707 34.0 31.0 34.0 30.0 34.0 4 35.224998924983254 37.0 35.0 37.0 33.0 37.0 5 35.27529861191063 37.0 35.0 37.0 33.0 37.0 6 35.34209668492577 37.0 37.0 37.0 33.0 37.0 7 35.285306671068454 37.0 37.0 37.0 33.0 37.0 8 35.28809339190672 37.0 37.0 37.0 33.0 37.0 9 36.923590583685545 39.0 38.0 39.0 34.0 39.0 10-11 36.895571485839604 39.0 38.0 39.0 34.0 39.0 12-13 36.83992029617752 39.0 38.0 39.0 34.0 39.0 14-15 38.336083526720756 41.0 39.0 41.0 34.0 41.0 16-17 38.34589877642287 41.0 39.0 41.0 34.0 41.0 18-19 38.354253390464116 41.0 39.0 41.0 34.0 41.0 20-21 38.391367130010444 41.0 39.0 41.0 34.0 41.0 22-23 38.376095650135454 41.0 39.0 41.0 34.0 41.0 24-25 38.38804012656068 41.0 39.0 41.0 34.0 41.0 26-27 38.33796862060787 41.0 39.0 41.0 34.0 41.0 28-29 38.276017832793976 41.0 39.0 41.0 33.0 41.0 30-31 38.177718301223855 41.0 39.0 41.0 33.0 41.0 32-33 38.07138180572298 40.0 38.0 41.0 33.0 41.0 34-35 38.009523261281785 40.0 38.0 41.0 33.0 41.0 36-37 38.172888355002435 41.0 39.0 41.0 33.0 41.0 38-39 38.11543183076046 41.0 39.0 41.0 33.0 41.0 40-41 38.01840289963228 41.0 38.5 41.0 33.0 41.0 42-43 37.970073614372765 41.0 38.0 41.0 33.0 41.0 44-45 37.907828063901775 41.0 38.0 41.0 33.0 41.0 46-47 37.785291412766206 40.0 38.0 41.0 32.5 41.0 48-49 37.690327900915364 40.0 38.0 41.0 32.0 41.0 50-51 37.585282119073014 40.0 38.0 41.0 32.0 41.0 52-53 37.49323224939279 40.0 38.0 41.0 31.5 41.0 54-55 37.35211584103068 40.0 38.0 41.0 31.0 41.0 56-57 37.13334438404314 40.0 37.5 41.0 30.5 41.0 58-59 36.964006358550684 40.0 37.0 41.0 30.5 41.0 60-61 36.74797446037627 40.0 36.5 41.0 30.5 41.0 62-63 36.488659613632045 40.0 36.0 41.0 30.0 41.0 64-65 36.19302792685447 39.0 36.0 41.0 29.5 41.0 66-67 35.85438655382921 39.0 35.0 41.0 29.0 41.0 68-69 35.40450938316232 39.0 35.0 40.5 28.5 41.0 70-71 34.962900825196726 37.5 34.5 40.0 28.0 41.0 72-73 34.56311874149523 37.0 34.0 39.5 27.5 41.0 74-75 34.120777785021616 37.0 34.0 39.0 26.0 41.0 76-77 33.61031336391467 36.0 34.0 39.0 26.0 40.5 78-79 33.09850482509131 36.0 34.0 38.5 25.5 40.0 80-81 32.615561526329586 35.0 33.0 37.0 23.5 39.0 82-83 32.37000411974161 35.0 34.0 37.0 23.5 39.0 84-85 31.86346246943138 35.0 34.0 37.0 18.5 39.0 86-87 31.13713607215235 35.0 34.0 36.0 2.0 37.0 88-89 15.631470387103127 18.0 16.0 18.5 2.0 19.5 90-91 2.7055140730095895 2.0 2.0 2.0 2.0 2.0 92-93 2.2762446266501506 2.0 2.0 2.0 2.0 2.0 94-95 2.0937907032551504 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17603012558846842 1101 2 0.33599024948712497 1101 3 0.3717289824989436 1101 4 0.27580380537155236 1101 5 0.21953775524563213 1101 6 0.22307182505163325 1101 7 0.34340573142470276 1101 8 0.3310941624154822 1101 9 0.30323850006529796 1101 10-11 0.3633842000181744 1101 12-13 0.4270555308776167 1101 14-15 0.38668847801091744 1101 16-17 0.39107464045493856 1101 18-19 0.5126985683564769 1101 20-21 0.39528251497697653 1101 22-23 0.4324313853185373 1101 24-25 0.3765004821827773 1101 26-27 0.500668744341624 1101 28-29 0.5093169100737285 1101 30-31 0.40448785471836146 1101 32-33 0.5552967583625517 1101 34-35 0.5540097843825649 1101 36-37 0.5417024334841329 1101 38-39 0.3848831090940692 1101 40-41 0.4183618003769709 1101 42-43 0.40609085066524386 1101 44-45 0.45708074006068955 1101 46-47 0.46740852292909807 1101 48-49 0.5410295850581832 1101 50-51 0.4551801067960497 1101 52-53 0.4497310051073029 1101 54-55 0.48189111954889086 1101 56-57 0.5242189467339031 1101 58-59 0.2874833969536752 1101 60-61 0.24771248602292673 1101 62-63 0.435668818684519 1101 64-65 0.48935918622341035 1101 66-67 0.34704441044463863 1101 68-69 0.3986771162580851 1101 70-71 0.3826059305458429 1101 72-73 0.48137204686653945 1101 74-75 0.3959131506036897 1101 76-77 0.32386720795999935 1101 78-79 0.40636193720420977 1101 80-81 0.5089303076774883 1101 82-83 0.47850626653492867 1101 84-85 0.3922467905759319 1101 86-87 0.5318725938937128 1101 88-89 0.8962466252173549 1101 90-91 0.01658144454927646 1101 92-93 -0.14287996001565828 1101 94-95 0.003775027257750807 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.10546306888279844 1102 2 0.10555720618279807 1102 3 0.15721753135382954 1102 4 0.12906913190420255 1102 5 0.1919749989700037 1102 6 0.026702188087931233 1102 7 0.1350448953410961 1102 8 0.0900600590051468 1102 9 0.3007182480400985 1102 10-11 0.06922478407658161 1102 12-13 0.061934018726397255 1102 14-15 0.1022300321663252 1102 16-17 0.06978751174207076 1102 18-19 0.14701199969961465 1102 20-21 0.18751173789926412 1102 22-23 0.18970711289129127 1102 24-25 0.21591442357691903 1102 26-27 0.12028570603779087 1102 28-29 0.1486358419669216 1102 30-31 0.11800420635352538 1102 32-33 0.21381761044776226 1102 34-35 0.19732411581571085 1102 36-37 0.10966923016380292 1102 38-39 0.09050867165032628 1102 40-41 -0.011111146917755832 1102 42-43 0.08674891647182648 1102 44-45 0.05659069105578851 1102 46-47 0.09434621669847587 1102 48-49 0.14889037113679393 1102 50-51 0.10642523130849924 1102 52-53 0.17976900890768377 1102 54-55 0.12832576301323684 1102 56-57 0.1436258874279659 1102 58-59 0.25895554416838706 1102 60-61 0.21727444221854597 1102 62-63 0.182968548657513 1102 64-65 0.32151740204499646 1102 66-67 0.2812278287864771 1102 68-69 0.24261150969743284 1102 70-71 0.319479617914574 1102 72-73 0.42281119078093354 1102 74-75 0.16392995228385843 1102 76-77 -0.005155694330227334 1102 78-79 0.05585688669916067 1102 80-81 0.26581099574629263 1102 82-83 0.3204204579540715 1102 84-85 0.5468422501218839 1102 86-87 0.5828376904033625 1102 88-89 1.3375757581306438 1102 90-91 0.013531139518773383 1102 92-93 -0.05772144416407343 1102 94-95 0.0551101607711022 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.011590764160079203 1103 2 0.00792233631283068 1103 3 0.16685040953526453 1103 4 0.16222117051232487 1103 5 0.15798606816202465 1103 6 0.07033165741784586 1103 7 0.0780500469200831 1103 8 0.15954892278514166 1103 9 0.24617853875001572 1103 10-11 0.25872054216302587 1103 12-13 0.25384469915614716 1103 14-15 0.2730843516408257 1103 16-17 0.3325640021570635 1103 18-19 0.3191245301015897 1103 20-21 0.34657200562816826 1103 22-23 0.3603488579168612 1103 24-25 0.3219335361264726 1103 26-27 0.2485303390117224 1103 28-29 0.33839492425812523 1103 30-31 0.3207676741896677 1103 32-33 0.31906207106403883 1103 34-35 0.21382065908706238 1103 36-37 0.13495409907622502 1103 38-39 0.12704945338806795 1103 40-41 0.3034789291111224 1103 42-43 0.23338506944882909 1103 44-45 0.20322301386253372 1103 46-47 0.2646576095398885 1103 48-49 0.22900508473581738 1103 50-51 0.2141420668218359 1103 52-53 0.13478365930888714 1103 54-55 0.07896827407285656 1103 56-57 0.040584732248625244 1103 58-59 0.10596609628743181 1103 60-61 0.19545587738333836 1103 62-63 0.028802280966914395 1103 64-65 0.16165662873362407 1103 66-67 0.17843276204575886 1103 68-69 -0.0032356282078538356 1103 70-71 -0.023648099112442367 1103 72-73 0.06974513949495531 1103 74-75 0.09816975924327664 1103 76-77 0.0874176055529503 1103 78-79 0.011562882830688181 1103 80-81 0.199248382038121 1103 82-83 0.2523296063157119 1103 84-85 0.44602929648610257 1103 86-87 0.3636370469347625 1103 88-89 0.37625952012386676 1103 90-91 -0.5675363289301987 1103 92-93 -0.29237490951060785 1103 94-95 -0.10380073031800663 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.056380184408048706 1104 2 0.03992616031958818 1104 3 0.12108460771663232 1104 4 0.19865298684142374 1104 5 0.19122009589348465 1104 6 0.07019826183324085 1104 7 0.12539806858945468 1104 8 0.005806806093644923 1104 9 0.0050845467389279975 1104 10-11 0.046552085772269436 1104 12-13 0.0297984775860769 1104 14-15 0.08197757588622068 1104 16-17 0.08216659447793262 1104 18-19 -0.07094824460067883 1104 20-21 0.021919575234726096 1104 22-23 0.14018129824086145 1104 24-25 0.1365405379124951 1104 26-27 0.13663182340815183 1104 28-29 0.2534478748943485 1104 30-31 0.25459234792629815 1104 32-33 0.2445078346425973 1104 34-35 0.22126857922756926 1104 36-37 0.17076896082652837 1104 38-39 0.14253723657577666 1104 40-41 0.20411589302761968 1104 42-43 0.13037681374430576 1104 44-45 0.15465127297603942 1104 46-47 0.12038657935543284 1104 48-49 0.1644360636370834 1104 50-51 0.07505123183959483 1104 52-53 0.1841789326586749 1104 54-55 0.19484827733363375 1104 56-57 0.21001657822118602 1104 58-59 0.3067885171208644 1104 60-61 0.0770663696871523 1104 62-63 -0.03853006672126469 1104 64-65 -0.1417275431391829 1104 66-67 -0.1396239977754945 1104 68-69 -0.09355555528492943 1104 70-71 -0.02107745503409575 1104 72-73 -0.07077877166359059 1104 74-75 -0.18839545267042723 1104 76-77 -0.12454797838622511 1104 78-79 -0.015311695676317072 1104 80-81 0.03696061070779422 1104 82-83 -0.0825090591355675 1104 84-85 -0.11859437974799647 1104 86-87 -0.11105669903557924 1104 88-89 0.20319542354546272 1104 90-91 -0.561501105084997 1104 92-93 -0.29237490951060785 1104 94-95 -0.10380073031800663 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.12881037727649414 1105 2 -0.26103836212285714 1105 3 -0.12586882055511595 1105 4 -0.05837998930142163 1105 5 -0.0682422080752545 1105 6 -0.21283284725944185 1105 7 -0.03422570998848329 1105 8 -0.22245449442574028 1105 9 -0.0867811698226717 1105 10-11 -0.13155341697964928 1105 12-13 0.050531114560165236 1105 14-15 -0.1864122375168975 1105 16-17 -0.13079741975202097 1105 18-19 -0.1018861309412955 1105 20-21 0.021484801245108542 1105 22-23 -0.14278313048864533 1105 24-25 -0.12345295624666619 1105 26-27 -0.13706133158224532 1105 28-29 -0.11874118736348294 1105 30-31 -0.07645943568330438 1105 32-33 -0.04182260079819855 1105 34-35 0.02430920378829171 1105 36-37 -0.1656262626968612 1105 38-39 -0.011011870808438573 1105 40-41 0.007729608277237787 1105 42-43 -0.01583204717486808 1105 44-45 -0.158964947292624 1105 46-47 0.007089433635414366 1105 48-49 0.0053087834389842214 1105 50-51 -0.12152247879647149 1105 52-53 -0.05162355650125505 1105 54-55 -0.05566173733604529 1105 56-57 -0.0192724108684601 1105 58-59 -0.28155981028332633 1105 60-61 -0.2117276348471151 1105 62-63 -0.21800431909021967 1105 64-65 -0.08135928731726239 1105 66-67 -0.022036331119636543 1105 68-69 -0.0506981034840166 1105 70-71 0.010719616796869502 1105 72-73 -0.10753632186309403 1105 74-75 -0.1066795686870563 1105 76-77 -0.0510085486655214 1105 78-79 -0.11074706438268578 1105 80-81 -0.25930519884587966 1105 82-83 -0.30652038758861977 1105 84-85 -0.27838711194884525 1105 86-87 -0.12289426567089023 1105 88-89 0.4585559840930351 1105 90-91 -0.6874589594911273 1105 92-93 -0.29237490951060785 1105 94-95 -0.10380073031800663 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.23491607107201418 1106 2 -0.14215858580456597 1106 3 -0.05748148956867283 1106 4 -0.15544748302259137 1106 5 -0.15271538843608568 1106 6 -0.16641487872078642 1106 7 -0.27603355944315666 1106 8 -0.20485718782158102 1106 9 -0.21809730698479513 1106 10-11 -0.10919877256479538 1106 12-13 -0.181664320699376 1106 14-15 -0.16890830615729868 1106 16-17 -0.33990866195693314 1106 18-19 -0.254751441950674 1106 20-21 -0.17821779423761797 1106 22-23 -0.10195327763756268 1106 24-25 -0.30378193381392293 1106 26-27 -0.42411995901851185 1106 28-29 -0.19751673247419888 1106 30-31 -0.14248184225133542 1106 32-33 -0.14046700168691473 1106 34-35 -0.24191889011753176 1106 36-37 -0.20241218205904943 1106 38-39 -0.27114657550704635 1106 40-41 -0.045532242088498265 1106 42-43 -0.5045358242471423 1106 44-45 -0.3040303710504233 1106 46-47 -0.46838822001049607 1106 48-49 -0.2625573042429892 1106 50-51 -0.36018785857191915 1106 52-53 -0.298238485719331 1106 54-55 -0.16490335581701032 1106 56-57 -0.5093205296627161 1106 58-59 -0.37659617426875514 1106 60-61 -0.030642492332056293 1106 62-63 -0.2131252443952576 1106 64-65 -0.05719822461971091 1106 66-67 -0.044596488339117 1106 68-69 -0.0713187608806507 1106 70-71 0.03554346919766971 1106 72-73 0.22929000192735316 1106 74-75 0.14105848146330402 1106 76-77 0.26869301707152005 1106 78-79 0.34936338020565216 1106 80-81 0.28683177129323667 1106 82-83 0.40639742299751447 1106 84-85 0.40775905608819585 1106 86-87 0.5338103386885962 1106 88-89 0.9951952955945949 1106 90-91 -0.16426786487146128 1106 92-93 -0.23625246053101634 1106 94-95 -0.10380073031800663 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.03210443102986105 1107 2 -0.018147876802899532 1107 3 0.05671263457119835 1107 4 0.07673353668103289 1107 5 0.08295310220528052 1107 6 0.06870172258026486 1107 7 0.03311840974497926 1107 8 0.052452410356011114 1107 9 0.1229820476569472 1107 10-11 0.18292682335826527 1107 12-13 0.1697482811653046 1107 14-15 0.2162928398937609 1107 16-17 0.210242814053494 1107 18-19 0.12789574811062465 1107 20-21 0.10014579515670619 1107 22-23 0.20261331309539798 1107 24-25 0.17386654790327327 1107 26-27 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0.006148204784700795 2216 9 -0.24621321553427578 2216 10-11 -0.16676358129691948 2216 12-13 -0.1474757743929942 2216 14-15 -0.11990272585655504 2216 16-17 -0.2562016241365015 2216 18-19 -0.2241229542627039 2216 20-21 -0.30220009320601804 2216 22-23 -0.30821830221830737 2216 24-25 -0.3616946992030563 2216 26-27 -0.2659704098461688 2216 28-29 -0.32365679585717544 2216 30-31 -0.3579752982117199 2216 32-33 -0.4605091607507603 2216 34-35 -0.33896667279622505 2216 36-37 -0.47753075442609827 2216 38-39 -0.47489757714576797 2216 40-41 -0.4211893531819655 2216 42-43 -0.5387702113164465 2216 44-45 -0.39120759071791866 2216 46-47 -0.5478215993049105 2216 48-49 -0.5976038308125808 2216 50-51 -0.5505208718621262 2216 52-53 -0.5865599197050244 2216 54-55 -0.4719920703265146 2216 56-57 -0.4936877466591696 2216 58-59 -0.43809534769121683 2216 60-61 -0.36479783115665754 2216 62-63 -0.7255975414145297 2216 64-65 -0.8427793194559499 2216 66-67 -0.9556812901409799 2216 68-69 -0.9361379605803393 2216 70-71 -0.9087748028582396 2216 72-73 -0.8468655823438525 2216 74-75 -1.010483500734594 2216 76-77 -0.9628010308483184 2216 78-79 -0.9095127631062923 2216 80-81 -0.9591591471823939 2216 82-83 -1.1717531368373173 2216 84-85 -1.0388147980732043 2216 86-87 -1.1033394487113277 2216 88-89 -1.4311263260855593 2216 90-91 -0.6874589594911273 2216 92-93 -0.29237490951060785 2216 94-95 -0.10380073031800663 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9459.0 3 1651.0 4 1125.0 5 1203.0 6 1055.0 7 932.0 8 1009.0 9 1296.0 10 1458.0 11 1924.0 12 2184.0 13 2708.0 14 3078.0 15 3509.0 16 3925.0 17 4620.0 18 5736.0 19 6849.0 20 7676.0 21 10453.0 22 12632.0 23 16093.0 24 24147.0 25 41190.0 26 71765.0 27 184244.0 28 288724.0 29 9888.0 30 386.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.32328042401435 21.101122317486432 15.751145413007563 23.824451845491655 2 13.039919990789508 20.708763976990415 45.4346339453779 20.816682086842174 3 18.618899921591485 26.567580507504317 30.084167724834682 24.729351846069513 4 11.226194871054684 19.065337289636357 40.063952213484924 29.644515625824035 5 11.406020349107523 38.4955095118407 34.336863639328655 15.761606499723118 6 26.99498834126996 37.27950019350301 21.83060787689047 13.894903588336554 7 26.30517436771676 32.362997784771935 23.520534207032966 17.811293640478336 8 23.315379397685454 39.605004168290755 21.24094385083484 15.838672583188956 9 24.244193869214158 20.47719646728689 23.60861622456892 31.66999343893003 10-11 22.80186569105127 26.7422190626463 31.626376293933035 18.829538952369393 12-13 26.186291618000784 24.760300290469438 29.94200321264194 19.11140487888784 14-15 20.137560530378586 31.226115555284295 26.116803690844602 22.519520223492513 16-17 19.210434510008454 31.401669127657815 30.80853442601803 18.5793619363157 18-19 20.704235316639547 28.88334463916367 31.808780260436393 18.60363978376039 20-21 21.163578960363935 29.42502841947492 28.89832312960321 20.513069490557935 22-23 19.76230059071133 31.82850020911056 29.361203644557822 19.04799555562029 24-25 20.913791979404067 29.570242981527745 31.114521881098987 18.4014431579692 26-27 18.708897948313194 31.571091897980217 30.40403984358853 19.315970310118058 28-29 18.605728616867363 32.172319036990906 27.938485824107683 21.283466522034047 30-31 19.765535379148005 29.52543905764725 30.151861720942296 20.557163842262447 32-33 18.80371283059985 30.810071500356152 31.408346863770632 18.977868805273367 34-35 19.278475499293958 31.21256509062057 30.710358182206576 18.7986012278789 36-37 18.51196565487729 30.71280694392909 30.251034272298842 20.524193128894776 38-39 20.137699242217224 31.617282940247097 28.87522731402557 19.369790503510103 40-41 22.103523419413275 28.43093329486392 31.082964937808548 18.38257834791426 42-43 18.8999041501195 31.644470460620404 30.723423852055504 18.732201537204595 44-45 18.559310102320858 31.779181696682773 29.433909658303953 20.227598542692412 46-47 21.06588951047205 29.580438301667733 29.43146178696913 19.922210400891085 48-49 19.681183825599895 30.256048893284525 29.62150982706747 20.44125745404811 50-51 18.03997261826406 31.940295609004348 30.146611579533612 19.873120193197984 52-53 18.222158106527917 30.47166186492519 30.876700433751918 20.429479594794977 54-55 18.935270340377247 29.52756670079039 30.546839671191528 20.99032328764083 56-57 19.932891212466313 30.41076736776254 27.70852203923048 21.947819380540672 58-59 19.848138279057704 31.381403458640982 27.488247639471286 21.28221062283003 60-61 19.127599633245897 28.372466254877455 32.26319461687097 20.23673949500568 62-63 19.88995989840745 28.47740176080808 31.122428455901424 20.510209884883047 64-65 20.806027310992853 30.309112611203627 28.274555306875882 20.61030477092764 66-67 20.392235466120326 32.11720040670311 27.45807781456724 20.032486312609322 68-69 21.244390808902576 28.737715264628978 30.666028588668564 19.35186533779988 70-71 22.429357528376975 28.587538960687674 29.498806384628505 19.48429712630684 72-73 20.688385241615215 31.046553080165733 28.51728141441688 19.747780263802177 74-75 19.994340556983516 31.3662838682293 30.279129832893847 18.360245741893333 76-77 20.39958719356821 29.571976879510736 30.875452027204165 19.152983899716887 78-79 19.9407005655987 29.141059830008288 30.270870106496233 20.64736949789678 80-81 20.47060765495153 30.254300413777415 29.79773039689618 19.477361534374875 82-83 19.924068402246046 29.690447655192315 31.338915151918677 19.04656879064296 84-85 19.724351643077544 30.077116900176648 30.864792622189608 19.333738834556193 86-87 18.095450128691425 32.756840134801756 28.22923233882708 20.918477397679737 88-89 28.312955495144966 19.419113510356407 35.744317181373496 16.52361381312513 90-91 30.222085898806206 15.412219428846704 17.473598837821967 36.892095834525115 92-93 22.078289445347902 17.70609853057414 37.078237061474226 23.137374962603737 94-95 15.369210319344878 7.013576366117129 27.882559281957732 49.73465403258026 96-97 26.33175749109375 7.268471199303142 15.018999101958702 51.38077220764441 98-99 57.216927411137085 14.058222941517313 18.54282802853204 10.182021618813563 100-101 30.414864565438215 7.07747380658454 12.91743282946439 49.590228798512854 102-103 22.584216376435716 2.103742126713598 37.58680992960356 37.72523156724713 104-105 39.87918747219405 4.6857686047700495 42.83787854085436 12.597165382181533 106-107 5.403043680561073 1.8619184342030102 6.319249785323302 86.41578809991262 108-109 17.78054445314651 11.032210608004684 22.711759772169838 48.475485166678965 110-111 39.883003938177104 2.8306856934138795 25.736402706771365 31.54990766163765 112-113 26.87332325111107 2.775653171554227 39.18290541832531 31.168118159009396 114-115 28.190094282827545 4.0252118876982115 23.73142219965344 44.053271629820806 116-117 19.425474568423837 4.241765328394735 20.143941398240624 56.1888187049408 118-119 19.960671068468997 4.321972192365036 15.518334513861715 60.199022225304255 120-121 25.124879377873643 4.215388545155248 30.067548390758926 40.59218368621218 122-123 33.996782811018875 1.6657570447821217 33.88245770258237 30.455002441616635 124-125 38.68985072137436 2.088927806966336 19.79095432685625 39.43026714480305 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 29.0 1 74.0 2 170.5 3 268.5 4 322.0 5 301.5 6 243.0 7 197.5 8 159.0 9 115.5 10 95.5 11 93.5 12 84.0 13 88.5 14 110.5 15 128.5 16 172.0 17 220.0 18 289.0 19 436.5 20 662.5 21 928.5 22 1300.5 23 1884.5 24 2648.5 25 3594.5 26 4687.5 27 6182.0 28 8307.5 29 11130.0 30 14873.5 31 19812.5 32 25904.5 33 32911.5 34 39394.5 35 44085.5 36 45812.5 37 44268.0 38 41374.5 39 38387.5 40 35011.0 41 31258.5 42 27835.5 43 25321.0 44 23515.5 45 21809.5 46 20077.0 47 18386.0 48 17133.0 49 16001.5 50 14787.0 51 13345.0 52 11705.0 53 10026.0 54 8463.0 55 7144.5 56 5926.5 57 4887.5 58 3903.0 59 3061.0 60 2417.5 61 1830.0 62 1383.5 63 1074.0 64 774.5 65 529.5 66 400.5 67 300.5 68 225.0 69 163.0 70 102.0 71 79.0 72 72.5 73 54.0 74 43.5 75 34.0 76 25.5 77 19.5 78 10.0 79 8.5 80 9.0 81 5.5 82 2.0 83 3.0 84 3.5 85 2.5 86 1.5 87 1.0 88 1.5 89 2.0 90 0.5 91 0.0 92 1.0 93 1.5 94 0.5 95 1.0 96 1.0 97 2.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 2.774236772785847E-4 3 0.046329754105523646 4 0.052849210521570385 5 0.05492988810115977 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.016269511554002E-4 12-13 0.00235810125686797 14-15 0.0 16-17 4.8549143523752327E-4 18-19 0.007213015609243202 20-21 0.003121016369384078 22-23 0.001179050628433985 24-25 0.0 26-27 0.0 28-29 0.0016645420636715084 30-31 0.0 32-33 0.0018726098216304467 34-35 1.3871183863929236E-4 36-37 2.0806775795893855E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 6.935591931964618E-5 46-47 0.0 48-49 2.774236772785847E-4 50-51 6.935591931964618E-5 52-53 0.0 54-55 0.0018032539023108004 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.935591931964618E-5 66-67 0.0 68-69 5.548473545571695E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.0806775795893855E-4 80-81 0.0 82-83 9.709828704750465E-4 84-85 6.935591931964618E-5 86-87 0.001179050628433985 88-89 19.252024152505342 90-91 26.28630955766182 92-93 40.42028299989319 94-95 44.796710864882186 96-97 48.56467924967992 98-99 93.05761118794206 100-101 51.79611024262088 102-103 53.20209343906874 104-105 74.90127184884848 106-107 81.6656933719322 108-109 12.595104304367064 110-111 39.04772935655739 112-113 52.121944351584574 114-115 86.75156293564187 116-117 66.44505138580062 118-119 71.99588303262918 120-121 80.4505083095327 122-123 75.85512380725157 124-125 50.10119028628737 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 720919.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.29256195218875 #Duplication Level Percentage of deduplicated Percentage of total 1 91.22095024713427 70.50700948314153 2 6.014608885876552 9.297690598595967 3 1.197739246776132 2.777290048020363 4 0.4900364145769492 1.515046797300692 5 0.25840667963592584 0.9986457147309595 6 0.1651502049665841 0.7658929469277825 7 0.10593168235969164 0.5731411785040234 8 0.07997019591144179 0.49448810574510293 9 0.06464113787996627 0.44966512388225416 >10 0.3401247806286883 4.841030226319822 >50 0.034369703228939986 1.830904321608885 >100 0.025371703621972854 3.9446190891854807 >500 0.0016194704418571867 0.8383810680963404 >1k 0.0010796469612381245 1.1661952979409114 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1835 0.25453622390310143 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1472 0.20418382647703834 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1452 0.2014095897042525 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1358 0.188370676872159 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1198 0.16617678268987224 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1070 0.14842166734404283 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 896 0.12428580742080596 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 820 0.11374370768421974 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA 803 0.11138560642735176 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.3871183863929234E-4 0.0 10-11 0.0 0.0 0.0 1.3871183863929234E-4 0.0 12-13 0.0 0.0 0.0 1.3871183863929234E-4 0.0 14-15 0.0 0.0 0.0 1.3871183863929234E-4 0.0 16-17 0.0 0.0 0.0 1.3871183863929234E-4 0.0 18-19 0.0 0.0 0.0 1.3871183863929234E-4 0.0 20-21 0.0 0.0 0.0 1.3871183863929234E-4 0.0 22-23 0.0 0.0 0.0 1.3871183863929234E-4 0.0 24-25 0.0 0.0 0.0 1.3871183863929234E-4 0.0 26-27 0.0 0.0 0.0 2.7742367727858467E-4 0.0 28-29 0.0 0.0 0.0 5.548473545571693E-4 0.0 30-31 0.0 0.0 0.0 0.0014564743057125695 0.0 32-33 0.0 0.0 0.0 0.003675863723941247 0.0 34-35 0.0 0.0 0.0 0.006242032738768156 0.0 36-37 0.0 0.0 0.0 0.01359376018665065 0.0 38-39 0.0 0.0 0.0 0.02413585992323687 0.0 40-41 0.0 0.0 0.0 0.03696670499737141 0.0 42-43 0.0 0.0 0.0 0.0534734137954472 0.0 44-45 0.0 0.0 0.0 0.0717833764958338 0.0 46-47 0.0 0.0 0.0 0.09363049108152233 0.0 48-49 0.0 0.0 0.0 0.11769699508543956 0.0 50-51 0.0 0.0 0.0 0.1419715668473157 0.0 52-53 0.0 0.0 0.0 0.17130912071952603 0.0 54-55 0.0 0.0 0.0 0.20522416526683301 1.3871183863929234E-4 56-57 0.0 0.0 0.0 0.24628286950406356 1.3871183863929234E-4 58-59 0.0 0.0 0.0 0.2919884203357104 1.3871183863929234E-4 60-61 0.0 0.0 0.0 0.346363461082313 2.7742367727858467E-4 62-63 0.0 0.0 0.0 0.4002530103936781 2.7742367727858467E-4 64-65 0.0 0.0 0.0 0.4605233042824506 2.7742367727858467E-4 66-67 0.0 0.0 0.0 0.527867901941827 2.7742367727858467E-4 68-69 0.0 0.0 0.0 0.6108175814481238 2.7742367727858467E-4 70-71 0.0 0.0 0.0 0.6973737687590422 2.7742367727858467E-4 72-73 0.0 0.0 0.0 0.7926688019042362 2.7742367727858467E-4 74-75 0.0 1.3871183863929234E-4 0.0 0.883663768051612 2.7742367727858467E-4 76-77 0.0 1.3871183863929234E-4 0.0 0.9880444266276793 2.7742367727858467E-4 78-79 0.0 1.3871183863929234E-4 0.0 1.0166190653873737 2.7742367727858467E-4 80-81 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 82-83 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 84-85 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 86-87 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 88-89 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 90-91 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 92-93 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 94-95 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 96-97 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 98-99 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 100-101 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 102-103 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 104-105 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 106-107 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 108-109 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 110-111 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 112-113 0.0 1.3871183863929234E-4 0.0 1.0170352009032915 2.7742367727858467E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGACG 5 2.2028826E-4 6082.7983 110-111 CTCGTCG 10 8.810574E-4 3041.3992 110-111 CGCGGCT 10 8.810574E-4 3041.3992 110-111 CCGCTCC 145 0.0 2524.4814 108-109 CCGCGCC 120 0.0 2033.61 108-109 CGGCGCT 15 0.001982164 2027.5994 110-111 CCACGCG 40 4.80577E-9 1830.249 108-109 CCGTTCG 15 0.003081613 1626.8881 108-109 CCGCGCT 35 7.952389E-6 1394.4755 108-109 CTCCGCC 90 2.910383E-11 1351.733 110-111 CGCGGCC 20 0.005477681 1220.166 108-109 CCGCTCT 40 1.186882E-5 1220.166 108-109 CACCGCG 20 0.005477681 1220.166 108-109 CGCGTCT 25 0.005504816 1216.5597 110-111 CCGATCT 30 0.007926076 1013.7997 110-111 CGCGTAT 30 0.007926076 1013.7997 110-111 CGGCACG 25 0.008557715 976.1328 108-109 ACGCTCG 25 0.008557715 976.1328 108-109 CCGCACA 25 0.008557715 976.1328 108-109 CCGCGCG 75 5.932816E-8 976.13275 108-109 >>END_MODULE