FastQCFastQC Report
Fri 27 May 2016
SRR939984_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939984_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720919
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22210.30807899361786834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17710.24565866623018676No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA15520.2152807735681817No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA14590.20238057257472752No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC13050.18101894942427652No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.16437352878756142No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA10400.14426031218486404No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10110.14023766886432457No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA9330.12941814545045977No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA8800.12206641800257727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8040.11152431826599106No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA7910.10972106436368025No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT7800.10819523413864802No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.10417259081810855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA358.885454E-785.111432
ACGTCGA401.9632444E-674.488021
GTCGGAC250.002045876371.49363
GTACACG350.00774842651.07751
CCGTATA350.007754812551.0668532
TCGAATA909.6177246E-846.3384444
GTCGAAT802.272116E-644.68353
TACCTGG8050.042.185662
TTGGACG1002.20125E-741.7045974
ACCTGGG7750.041.512413
GTACCTG8700.040.411891
TACGACG600.001299788639.7186664
TCCCGTC758.122662E-539.7186663
CGACGTG650.001939164536.6125266
ACGTTGA902.370879E-433.1057851
TAGGACC15350.032.214814
ACGACGT750.003884533231.7749335
TACATGG29650.030.9443912
GTACATG30450.030.9204771
ACATGGG30050.029.9376953
Produced by FastQC (version 0.11.5)