##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939984_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 720919 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.844085119132664 34.0 31.0 34.0 31.0 34.0 2 32.952707585734316 34.0 33.0 34.0 31.0 34.0 3 32.978431696209974 34.0 33.0 34.0 31.0 34.0 4 36.368231382443795 37.0 37.0 37.0 35.0 37.0 5 36.266118662429484 37.0 37.0 37.0 35.0 37.0 6 36.405933260185954 37.0 37.0 37.0 35.0 37.0 7 36.40639100925347 37.0 37.0 37.0 35.0 37.0 8 36.43655944703912 37.0 37.0 37.0 35.0 37.0 9 38.27490605740728 39.0 39.0 39.0 37.0 39.0 10-11 38.26801554682287 39.0 39.0 39.0 37.0 39.0 12-13 38.254901036038724 39.0 39.0 39.0 37.0 39.0 14-15 39.797760219941495 41.0 40.0 41.0 38.0 41.0 16-17 39.82472094645862 41.0 40.0 41.0 38.0 41.0 18-19 39.86488010442227 41.0 40.0 41.0 38.0 41.0 20-21 39.870667855889494 41.0 40.0 41.0 38.0 41.0 22-23 39.812492110764175 41.0 40.0 41.0 37.5 41.0 24-25 39.78803097157933 41.0 40.0 41.0 37.0 41.0 26-27 39.69515992781436 41.0 40.0 41.0 37.0 41.0 28-29 39.615076728453545 41.0 40.0 41.0 37.0 41.0 30-31 39.49256435188974 41.0 40.0 41.0 36.5 41.0 32-33 39.350798078563614 41.0 39.0 41.0 36.0 41.0 34-35 39.252778744907545 41.0 39.0 41.0 35.0 41.0 36-37 39.228497931112926 41.0 39.5 41.0 35.5 41.0 38-39 39.27430890294194 41.0 40.0 41.0 35.5 41.0 40-41 39.185804507857334 41.0 40.0 41.0 35.0 41.0 42-43 39.11137173524349 41.0 40.0 41.0 35.0 41.0 44-45 38.99733326490216 41.0 40.0 41.0 35.0 41.0 46-47 38.808701116214166 41.0 39.5 41.0 35.0 41.0 48-49 38.61756313816115 41.0 39.0 41.0 35.0 41.0 50-51 38.497297893383305 41.0 39.0 41.0 35.0 41.0 52-53 38.4201491429689 41.0 39.0 41.0 35.0 41.0 54-55 38.331470664526805 41.0 39.0 41.0 35.0 41.0 56-57 38.189597583084925 41.0 39.0 41.0 34.5 41.0 58-59 38.04875582416332 41.0 38.5 41.0 34.0 41.0 60-61 37.876794064243 40.0 38.0 41.0 34.0 41.0 62-63 37.689054526236646 40.0 37.5 41.0 34.0 41.0 64-65 37.45122544973846 40.0 37.0 41.0 33.5 41.0 66-67 37.17024450735797 39.0 36.5 41.0 33.0 41.0 68-69 36.81801284194202 39.0 36.0 41.0 32.5 41.0 70-71 36.44620061338375 39.0 35.5 41.0 32.0 41.0 72-73 36.09952227642773 37.5 35.0 40.0 32.0 41.0 74-75 35.742987076218 37.0 35.0 39.5 32.0 41.0 76-77 35.33857062998756 36.5 35.0 39.0 31.0 41.0 78-79 34.945900302253094 36.0 35.0 39.0 31.0 40.5 80-81 34.648871787260425 36.0 35.0 37.5 31.0 39.0 82-83 34.387610119860895 35.0 35.0 37.0 31.0 39.0 84-85 34.07473169662612 35.0 35.0 37.0 31.0 39.0 86-87 33.83208030305762 35.0 35.0 36.0 31.0 37.0 88-89 33.57990564820736 35.0 35.0 36.0 31.0 37.0 90-91 33.35748052139006 35.0 34.5 36.0 31.0 37.0 92-93 33.220051767258184 35.0 34.0 35.5 31.0 36.0 94-95 33.106704081873275 35.0 34.0 35.0 30.5 36.0 96-97 32.94231252054669 35.0 34.0 35.0 30.0 36.0 98-99 32.79239345890454 35.0 34.0 35.0 29.5 36.0 100-101 32.80400502691703 35.0 34.0 35.0 30.0 36.0 102-103 31.95645280537758 34.5 32.5 35.0 28.0 35.0 104-105 32.63653198209508 35.0 34.0 35.0 29.0 35.0 106-107 32.697536755169445 35.0 34.0 35.0 30.0 35.0 108-109 32.70577901262139 35.0 34.0 35.0 30.0 35.0 110-111 32.69204029856336 35.0 34.0 35.0 30.0 35.0 112-113 32.590304874750146 35.0 34.0 35.0 29.0 35.5 114-115 32.505559570492665 35.0 34.0 35.0 29.0 35.0 116-117 32.45585703803063 35.0 34.0 35.0 29.0 35.0 118-119 32.36340698469592 35.0 34.0 35.0 29.0 35.0 120-121 32.2552804129174 35.0 34.0 35.0 29.0 35.0 122-123 32.07217176964403 35.0 34.0 35.0 29.0 35.0 124-125 29.984452483566116 33.5 29.5 34.5 22.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12397089485428125 1101 2 0.14458283505287994 1101 3 0.12438470424542203 1101 4 0.06839523552042692 1101 5 -0.004543738378046669 1101 6 0.023250165808384793 1101 7 0.09755337348035908 1101 8 0.12106692653492956 1101 9 0.16279083517915183 1101 10-11 0.07310919944330863 1101 12-13 0.06769150306312355 1101 14-15 0.21458738872386363 1101 16-17 0.30507568108573935 1101 18-19 0.25291460452819337 1101 20-21 0.2326289009892406 1101 22-23 0.20940154174092385 1101 24-25 0.3046087951685621 1101 26-27 0.2712121025565253 1101 28-29 0.2427506713222911 1101 30-31 0.32240675100231186 1101 32-33 0.32616145655459405 1101 34-35 0.31099453378072894 1101 36-37 0.3855840288419685 1101 38-39 0.3079658620456982 1101 40-41 0.32324560586167195 1101 42-43 0.3035570170265913 1101 44-45 0.31486909965474297 1101 46-47 0.3506963872408093 1101 48-49 0.29437064362832643 1101 50-51 0.285725335237494 1101 52-53 0.2336086762759706 1101 54-55 0.2055694414945748 1101 56-57 0.22064433605056877 1101 58-59 0.19273873941916975 1101 60-61 0.22036396587916585 1101 62-63 0.24143347651794755 1101 64-65 0.29892934945225136 1101 66-67 0.25250695678725776 1101 68-69 0.227865162089401 1101 70-71 0.2858933450910399 1101 72-73 0.3164867964820388 1101 74-75 0.3326430411683958 1101 76-77 0.40258134139754276 1101 78-79 0.30397677010078894 1101 80-81 0.32068855136559904 1101 82-83 0.3050692685729288 1101 84-85 0.31269758302423867 1101 86-87 0.2765217152940025 1101 88-89 0.2338220437523333 1101 90-91 0.19693619452616673 1101 92-93 0.2891348166335632 1101 94-95 0.23944475521761888 1101 96-97 0.2645767147235958 1101 98-99 0.18541382961215902 1101 100-101 0.3103586754941787 1101 102-103 0.39996413137279063 1101 104-105 0.32311472073145353 1101 106-107 0.32248713206418245 1101 108-109 0.3005548063718777 1101 110-111 0.27676752845987806 1101 112-113 0.2022548724891422 1101 114-115 0.3115971448682373 1101 116-117 0.28263906957566576 1101 118-119 0.3189544803485873 1101 120-121 0.2966365377610529 1101 122-123 0.3352564899149826 1101 124-125 0.42912622202256046 1102 1 0.05132363012781127 1102 2 0.11299967673704714 1102 3 0.10058232400739797 1102 4 0.021220518048679082 1102 5 -0.045387822786487675 1102 6 0.007778618653667024 1102 7 0.007274345577570784 1102 8 0.03667848769104154 1102 9 0.030067562851918694 1102 10-11 0.134130301553526 1102 12-13 0.003895123425159852 1102 14-15 0.07224815480046942 1102 16-17 0.20046522003963219 1102 18-19 0.11040535360309889 1102 20-21 0.10617125522466608 1102 22-23 0.269642565843327 1102 24-25 0.19479781407045493 1102 26-27 0.1529302743737091 1102 28-29 0.19921131738690434 1102 30-31 0.13334284461167556 1102 32-33 0.07298613902284501 1102 34-35 0.19606804113146836 1102 36-37 0.21924239467855244 1102 38-39 0.20464052951244582 1102 40-41 0.20800408170926232 1102 42-43 0.028324493774263715 1102 44-45 -0.053392726527874856 1102 46-47 -0.09316799919783847 1102 48-49 -0.03399219265530462 1102 50-51 -0.12523576087211552 1102 52-53 -0.08229791438309775 1102 54-55 -0.019433058755453203 1102 56-57 -0.08918164654768646 1102 58-59 -0.0743779722519946 1102 60-61 -0.03194126464388347 1102 62-63 -0.040049671796886344 1102 64-65 -0.04059460294299555 1102 66-67 -0.029776271535574494 1102 68-69 0.10579795536872894 1102 70-71 0.1261073664931871 1102 72-73 0.048900037806163255 1102 74-75 0.047609537818061654 1102 76-77 0.14177026028944084 1102 78-79 0.1661629887226539 1102 80-81 0.1262591084213014 1102 82-83 0.17399116076214938 1102 84-85 0.17370868413434692 1102 86-87 0.17233129625209642 1102 88-89 0.13485714726268583 1102 90-91 0.232389739880702 1102 92-93 0.18154905805771193 1102 94-95 0.13194900564266732 1102 96-97 0.1743376908212042 1102 98-99 0.1937446626954724 1102 100-101 0.1809907387005012 1102 102-103 0.1251616511247633 1102 104-105 0.11479388864824358 1102 106-107 0.15702558590956528 1102 108-109 0.1477795288441257 1102 110-111 0.05038489019605663 1102 112-113 0.13482812981487768 1102 114-115 0.12579856501024977 1102 116-117 0.08494430009871934 1102 118-119 0.11567915281582941 1102 120-121 0.11695856996427523 1102 122-123 0.30535349961595415 1102 124-125 0.2575990693072896 1103 1 0.03052580566451013 1103 2 0.02255403642833187 1103 3 0.07942446354050503 1103 4 -0.0505817685629637 1103 5 -0.18689920796755644 1103 6 -0.025084242100490428 1103 7 -0.07884894760281469 1103 8 -0.06530745558413997 1103 9 -0.030546796456356162 1103 10-11 0.032382786376473405 1103 12-13 0.035626168870130925 1103 14-15 -0.06691916618694194 1103 16-17 -0.06759786286440317 1103 18-19 -0.026442799297299757 1103 20-21 -0.004207548613166523 1103 22-23 -0.013465425806899134 1103 24-25 0.12259718975270317 1103 26-27 0.06297010814432014 1103 28-29 0.10499008675319743 1103 30-31 0.06012435254970683 1103 32-33 0.10550811462465504 1103 34-35 0.20270955634681798 1103 36-37 0.24872608772871274 1103 38-39 0.3006265110893338 1103 40-41 0.2750186555070684 1103 42-43 0.22796062760041735 1103 44-45 0.17591573623329282 1103 46-47 0.13815609065795087 1103 48-49 0.2895135621754932 1103 50-51 0.23460777667312982 1103 52-53 0.05242449402795302 1103 54-55 0.2377207417309748 1103 56-57 0.22496412973206503 1103 58-59 0.24009528358423182 1103 60-61 0.19954943761783284 1103 62-63 0.09602191408510663 1103 64-65 0.1873991538795039 1103 66-67 0.2651439651689458 1103 68-69 0.2600379538366653 1103 70-71 0.24271689978694155 1103 72-73 0.25635354911474906 1103 74-75 0.2305691103710572 1103 76-77 0.28186352493505495 1103 78-79 0.3103382573133473 1103 80-81 0.18517382968065732 1103 82-83 0.260528112329645 1103 84-85 0.1112146687753679 1103 86-87 0.1745767335617856 1103 88-89 0.1025841327271877 1103 90-91 0.1034911053363956 1103 92-93 0.20948727741156148 1103 94-95 0.21714731270741083 1103 96-97 0.20015591094109197 1103 98-99 0.0920009776886701 1103 100-101 0.1640767068717821 1103 102-103 0.077419536487767 1103 104-105 0.12370573727991285 1103 106-107 0.10647595647853336 1103 108-109 0.24913636665556282 1103 110-111 0.28960870619467016 1103 112-113 0.14817148542703507 1103 114-115 0.24037427790068477 1103 116-117 0.11061027095112053 1103 118-119 0.15611330261378953 1103 120-121 0.15036531489408134 1103 122-123 0.1799910697559497 1103 124-125 0.22445181841198547 1104 1 0.06237214877278063 1104 2 0.17223870408805908 1104 3 0.0903721650604723 1104 4 0.03645514495964619 1104 5 0.03646984573898493 1104 6 0.011477264380310714 1104 7 -0.011154393848094912 1104 8 0.09010210591493006 1104 9 0.012947574991059696 1104 10-11 0.10563271041509381 1104 12-13 0.10373295203560673 1104 14-15 0.08172427482627143 1104 16-17 0.13010180438772068 1104 18-19 0.11539283510987275 1104 20-21 0.20341434160226868 1104 22-23 0.179925749334096 1104 24-25 0.1976599968404571 1104 26-27 0.18591081380696295 1104 28-29 0.11949222478798305 1104 30-31 0.018836566397645527 1104 32-33 0.19205313192902906 1104 34-35 0.18048251706181873 1104 36-37 0.02900966450899034 1104 38-39 0.07758446183671452 1104 40-41 -0.12030193854793936 1104 42-43 -0.1230800432311554 1104 44-45 -0.060514042107712385 1104 46-47 -0.07642830798734224 1104 48-49 -0.11496408294777893 1104 50-51 -0.1777717041216178 1104 52-53 -0.20473162327122907 1104 54-55 -0.31874264488648407 1104 56-57 -0.17326893458684367 1104 58-59 -0.23631037238855868 1104 60-61 -0.20486243607138022 1104 62-63 -0.20325478541169417 1104 64-65 -0.2310340779249671 1104 66-67 -0.3162060352015956 1104 68-69 -0.2243640263466844 1104 70-71 -0.2220916775491517 1104 72-73 -0.24032267756881254 1104 74-75 -0.3677749316633765 1104 76-77 -0.29979587908312055 1104 78-79 -0.25494869141087406 1104 80-81 -0.30364965831744684 1104 82-83 -0.1829575513504409 1104 84-85 -0.3331575197274148 1104 86-87 -0.2924699252493639 1104 88-89 -0.20911595694428797 1104 90-91 -0.19599309840312173 1104 92-93 -0.11549771558517108 1104 94-95 -0.11313274391159922 1104 96-97 -0.12161276629346673 1104 98-99 -0.15307450197961714 1104 100-101 -0.06304780308471436 1104 102-103 -0.09911284529039577 1104 104-105 -0.01442446422152699 1104 106-107 -0.020581485142372458 1104 108-109 -0.01153157468002064 1104 110-111 -0.04032581880588282 1104 112-113 -0.06017111148856458 1104 114-115 -0.053798187768478556 1104 116-117 -0.08849468173050212 1104 118-119 -0.1487484106301622 1104 120-121 -0.2081353187349393 1104 122-123 -0.2257065891515424 1104 124-125 -0.0787804307116815 1105 1 0.01793689540541976 1105 2 0.06562581423320779 1105 3 -0.02467218639189639 1105 4 0.015187713451190632 1105 5 -0.1119489285678057 1105 6 0.01903016713871608 1105 7 0.0033260579924601075 1105 8 0.0712099370730428 1105 9 0.030115065250321038 1105 10-11 0.060813143873502895 1105 12-13 0.06112014508067176 1105 14-15 -0.0031535198919243612 1105 16-17 -0.015428989324860254 1105 18-19 -0.0734121626734563 1105 20-21 0.024151844440680748 1105 22-23 0.09896272269439521 1105 24-25 0.0026631866848561003 1105 26-27 0.11218485928657174 1105 28-29 0.07973766258962911 1105 30-31 0.07164561185923901 1105 32-33 0.08530462863285493 1105 34-35 0.08968548848297075 1105 36-37 0.1977727077286815 1105 38-39 0.12049282499599201 1105 40-41 0.019447036917298988 1105 42-43 0.06463914593562947 1105 44-45 0.1511605180722171 1105 46-47 0.14274148544770782 1105 48-49 0.11510479606520363 1105 50-51 0.07438189139170248 1105 52-53 0.19267297875513378 1105 54-55 0.0973175909096966 1105 56-57 0.16208508407645184 1105 58-59 0.13819898629051863 1105 60-61 0.2187898997237312 1105 62-63 0.10115819311001673 1105 64-65 0.10235827654063456 1105 66-67 0.28081164050116314 1105 68-69 0.31876285016488737 1105 70-71 0.2651962518633795 1105 72-73 0.2504862453450727 1105 74-75 0.1759628811486209 1105 76-77 0.13639596933258957 1105 78-79 0.24852003910314124 1105 80-81 0.19772134401456753 1105 82-83 0.20846113645210096 1105 84-85 0.06222008936706658 1105 86-87 0.036025026866923326 1105 88-89 0.09641932417652299 1105 90-91 0.16462151432813243 1105 92-93 0.020941120500076238 1105 94-95 0.04724524363899718 1105 96-97 -0.01210829150045356 1105 98-99 0.05842569806146969 1105 100-101 0.11337852592701125 1105 102-103 0.06202025231884534 1105 104-105 0.28376876826226294 1105 106-107 0.20089824222505115 1105 108-109 0.2990439309193249 1105 110-111 0.26387044853551345 1105 112-113 0.20388357722227113 1105 114-115 0.24922982158839346 1105 116-117 0.26299958916477806 1105 118-119 0.23344225556073184 1105 120-121 -0.043798860435394715 1105 122-123 0.06015077614511455 1105 124-125 -0.0352057144636575 1106 1 0.017992746018990147 1106 2 -0.02272287510997728 1106 3 -0.07132752003823128 1106 4 0.04423526850331427 1106 5 -0.10112176352750168 1106 6 -0.02216315103350297 1106 7 0.02352739945438742 1106 8 0.00843035675549686 1106 9 -0.05875111493423191 1106 10-11 -0.01661456885188528 1106 12-13 -0.06664162412714347 1106 14-15 -0.2874121989900047 1106 16-17 -0.12506243476666157 1106 18-19 -0.10813466366393243 1106 20-21 0.0394213149245104 1106 22-23 -0.07992024758086558 1106 24-25 0.017595808155576265 1106 26-27 0.05400946392531836 1106 28-29 -0.030337351765730602 1106 30-31 -0.0304689447305293 1106 32-33 0.06427730895616435 1106 34-35 -0.003414866342957623 1106 36-37 0.04279903605698365 1106 38-39 0.08238350789191884 1106 40-41 0.0628250740125722 1106 42-43 0.07040921245021536 1106 44-45 0.006097712311927239 1106 46-47 0.10205693145848471 1106 48-49 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-0.14327972633010688 2216 36-37 -0.20698367150782815 2216 38-39 -0.16862101843223343 2216 40-41 -0.16571206422439388 2216 42-43 -0.2677671192780906 2216 44-45 -0.2443181031122279 2216 46-47 -0.19538898005078664 2216 48-49 -0.1387307988116433 2216 50-51 -0.1796777225780133 2216 52-53 -0.11237843216250809 2216 54-55 -0.10219300790211605 2216 56-57 -0.29200747387750425 2216 58-59 -0.2486007252665985 2216 60-61 -0.3321396696983996 2216 62-63 -0.4615005328362969 2216 64-65 -0.3871080675190157 2216 66-67 -0.40814626806830034 2216 68-69 -0.44071165668196954 2216 70-71 -0.4589795079530177 2216 72-73 -0.2643487968049243 2216 74-75 -0.3353815628484256 2216 76-77 -0.45933156195504665 2216 78-79 -0.46651413112940787 2216 80-81 -0.38963255856641155 2216 82-83 -0.3154452147820237 2216 84-85 -0.19369317278285791 2216 86-87 -0.29540573036885576 2216 88-89 -0.3842359682745453 2216 90-91 -0.48289322810481394 2216 92-93 -0.3831483260775954 2216 94-95 -0.30008281621655897 2216 96-97 -0.3697313464327152 2216 98-99 -0.3475755977352435 2216 100-101 -0.447138474999079 2216 102-103 -0.3801875802964325 2216 104-105 -0.3416398286534488 2216 106-107 -0.32310694096180725 2216 108-109 -0.2963087710364505 2216 110-111 -0.3969953925793064 2216 112-113 -0.46583065201661356 2216 114-115 -0.5395190757032395 2216 116-117 -0.48839815087789873 2216 118-119 -0.4959631787324952 2216 120-121 -0.567762314575539 2216 122-123 -0.65889121279303 2216 124-125 -0.5771584142231703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58.0 3 5.0 4 10.0 5 24.0 6 68.0 7 165.0 8 263.0 9 493.0 10 1368.0 11 1941.0 12 1847.0 13 2636.0 14 4462.0 15 4852.0 16 1908.0 17 1235.0 18 1087.0 19 1058.0 20 1016.0 21 1049.0 22 1062.0 23 1296.0 24 1520.0 25 1923.0 26 2528.0 27 3229.0 28 4163.0 29 5288.0 30 6627.0 31 8802.0 32 11354.0 33 15516.0 34 21967.0 35 36786.0 36 77732.0 37 248884.0 38 243740.0 39 2956.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.31333294486852 20.99041879281784 16.087299142880134 23.608949119433507 2 13.28803929428965 20.97212030755189 44.918222435530204 20.821617962628256 3 18.695720323642462 26.71465171537995 30.26539736086856 24.32423060010903 4 11.270336889442504 19.065109949938897 39.74968061599154 29.914872544627062 5 11.096279525234918 37.93106322098523 34.52950613597061 16.443151117809247 6 26.792330345017955 37.51114896403063 21.48535411051727 14.21116658043414 7 26.006527779126365 32.39240469456347 23.73442786221476 17.866639664095413 8 23.543421660408452 38.60530794721737 21.294347908710964 16.556922483663215 9 24.361682796541636 19.632718793650884 23.96871215767652 32.036886252130955 10-11 22.813728033246452 26.29768392843024 31.752596338839734 19.135991699483576 12-13 26.53800218887281 24.39656882395942 30.323240197581143 18.742188789586624 14-15 20.226058683430452 30.457235833706697 27.256113377508427 22.060592105354417 16-17 18.672637753088594 30.80715781326183 31.763091112240843 18.75711332140873 18-19 20.12729585431928 27.71226725887374 33.814270396535534 18.346166490271447 20-21 20.538776585968087 28.245889643740224 31.119827158695646 20.095506611596043 22-23 19.143908582969445 30.551687020401825 31.6999483989813 18.604455997647438 24-25 20.347639609997795 28.3994457074928 33.39709454182786 17.855820140681548 26-27 18.040792377507042 30.39641069246337 32.649299019723436 18.913497910306152 28-29 18.073736439183875 30.918868832698266 30.289880000388393 20.717514727729466 30-31 19.208399279253285 28.29499569299741 32.410575945425215 20.08602908232409 32-33 18.29435761853967 29.503800010819525 33.66127123851639 18.540571132124413 34-35 18.76882146260537 29.979859041029577 32.81901295429861 18.432306542066446 36-37 18.12034361696668 29.509556552123055 32.30869209994465 20.061407730965612 38-39 19.702421492567126 30.30999321699109 31.00604922328306 18.981536067158725 40-41 21.69279766520233 27.200697997972036 32.9969108873535 18.109593449472133 42-43 18.62580955696826 30.4413533281825 32.58937550543126 18.34346160941798 44-45 18.417048239816122 30.61481248240094 31.050437011647634 19.917702266135308 46-47 20.855394295336925 28.672430605934924 30.855754946117386 19.616420152610765 48-49 19.74015111267701 29.451852427249108 30.58505879301281 20.222937667061068 50-51 18.06541372886552 31.127491438011763 31.028867320739224 19.778227512383502 52-53 18.26696203040841 29.73725203524945 31.82604425739924 20.169741676942902 54-55 18.983478032899672 28.759819064277682 31.40463769161307 20.85206521120958 56-57 19.841896246318935 29.701395024961197 28.69067121271599 21.766037516003877 58-59 19.78419212144499 30.5550276799474 28.44223830971302 21.218541888894592 60-61 18.982784473706477 27.752077556563222 33.16239411085018 20.102743858880125 62-63 19.776632326239145 27.76546324899191 32.078569159642065 20.379335265126873 64-65 20.753302382098404 29.62364703940387 29.258418768266615 20.364631810231106 66-67 20.291253247590923 31.368572613566847 28.471229083988632 19.868945054853597 68-69 21.16770400003329 28.01070577970618 31.680327470908658 19.14126274935187 70-71 22.274693828294165 27.8939104115719 30.44905183522698 19.38234392490696 72-73 20.49176121034402 30.25693593871156 29.608041957556953 19.643260893387467 74-75 19.870956376513867 30.585197504851447 31.294639203572107 18.24920691506258 76-77 20.187011300853495 28.723199138876904 31.916623088030693 19.173166472238908 78-79 19.69576332431244 28.436759192086768 31.29651181339374 20.570965670207055 80-81 20.260667287170957 29.445749106348977 30.82447542650423 19.469108179975837 82-83 19.808050557690947 28.98321448040626 32.15062995981518 19.058105002087615 84-85 19.498653801606007 29.305164657888056 31.781309689438064 19.414871851067872 86-87 17.91733884111807 31.87507889235823 29.15757526157585 21.05000700494785 88-89 19.13335617454943 29.433265040871444 30.859153386164053 20.574225398415077 90-91 19.799836317986987 29.09107933029088 32.0175888807202 19.091495471001927 92-93 18.836027348426107 30.810812310398255 31.514220044138106 18.83894029703753 94-95 18.823752169130447 31.35692200562899 30.51444630474497 19.304879520495597 96-97 20.747476484875556 30.165663548886908 29.994631851844662 19.092228114392878 98-99 20.470034193132243 28.632898925655947 32.253417579292694 18.643649301919115 100-101 19.42940766453859 29.092233161214704 32.001650674314334 19.47670849993238 102-103 18.923147773110877 30.531920501140213 30.779868944509488 19.765062781239422 104-105 19.556054623425318 29.731189992502422 30.801033986013305 19.91172139805895 106-107 19.431607027298096 28.88609598480734 30.95906430314847 20.723232684746094 108-109 18.718074448392358 30.042425221818647 30.5956800762364 20.6438202535526 110-111 18.37494113085631 30.33839960548137 30.887521267453533 20.399137996208793 112-113 19.597075694834608 28.506010223970147 30.44960498297161 21.447309098223638 114-115 19.4384082221786 29.595514923090477 28.42860700019906 22.53746985453186 116-117 19.761881973025496 29.660717183865238 27.561474983458247 23.01592585965102 118-119 19.317603323554096 28.352787776644956 30.216391668920746 22.1132172308802 120-121 19.119739996005062 27.760531105044723 31.105946356836895 22.013782542113322 122-123 20.14863159062851 29.138241944209405 29.55854406236562 21.154582402796464 124-125 21.1746852580357 29.695536739778166 28.249166329129377 20.880611673056755 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 133.0 1 95.0 2 358.5 3 459.5 4 154.5 5 52.5 6 99.0 7 261.0 8 474.5 9 489.5 10 326.5 11 230.0 12 238.0 13 294.0 14 358.5 15 429.0 16 520.0 17 649.0 18 777.5 19 925.5 20 1142.5 21 1389.5 22 1727.0 23 2135.0 24 2697.5 25 3424.5 26 4286.5 27 5402.5 28 6584.5 29 8047.5 30 10186.5 31 13580.5 32 18783.5 33 26516.5 34 36761.5 35 47065.5 36 54344.0 37 55707.5 38 49862.0 39 40247.5 40 32005.5 41 26736.0 42 22938.5 43 20324.5 44 19047.5 45 18385.5 46 17941.0 47 18507.5 48 20658.5 49 19657.5 50 16447.5 51 14579.0 52 12696.0 53 11116.0 54 10020.0 55 8732.5 56 7300.0 57 6050.5 58 4875.5 59 3909.0 60 3062.5 61 2333.0 62 1773.5 63 1260.5 64 865.0 65 661.0 66 486.5 67 363.5 68 263.5 69 193.5 70 135.5 71 103.5 72 94.0 73 75.5 74 50.5 75 39.5 76 25.5 77 15.5 78 14.0 79 7.0 80 5.0 81 3.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 2.5 99 3.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018448674539025885 2 0.0 3 0.0 4 0.0 5 0.13649244922106366 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.935591931964618E-5 18-19 0.0 20-21 0.0036758637239412474 22-23 4.161355159178771E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0012484065477536312 92-93 0.0 94-95 0.001109694709114339 96-97 0.0 98-99 0.001941965740950093 100-101 2.0806775795893855E-4 102-103 4.161355159178771E-4 104-105 0.0027048808534662007 106-107 0.00617267681944851 108-109 4.8549143523752327E-4 110-111 0.00561782946489134 112-113 0.008877557672914711 114-115 0.005479117626252048 116-117 0.002219389418228678 118-119 5.548473545571695E-4 120-121 9.709828704750465E-4 122-123 0.0012484065477536312 124-125 0.0015258302250322157 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 720919.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.62923172620808 #Duplication Level Percentage of deduplicated Percentage of total 1 90.7612360265206 68.64202551206793 2 6.283635775323063 9.504530922699983 3 1.271143419562279 2.8840680070596045 4 0.5120552295216675 1.5490537444044343 5 0.29705710054092677 1.123310014636262 6 0.16997905997803875 0.771323142940927 7 0.13186136009026725 0.6980801350599852 8 0.08625185535374162 0.5218529244290805 9 0.06155915029134085 0.41901041180270576 >10 0.3566887963524864 5.0157864592624195 >50 0.03738161437635123 1.9512739056875963 >100 0.02748583420021796 4.171323262281324 >500 0.0021988667333881964 1.1485690091745793 >1k 0.001465911155592131 1.5997925484931639 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2221 0.30807899361786834 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1771 0.24565866623018676 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1552 0.2152807735681817 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1459 0.20238057257472752 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1305 0.18101894942427652 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1185 0.16437352878756142 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1040 0.14426031218486404 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1011 0.14023766886432457 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 933 0.12941814545045977 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 880 0.12206641800257727 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 804 0.11152431826599106 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA 791 0.10972106436368025 No Hit ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT 780 0.10819523413864802 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 751 0.10417259081810855 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3871183863929234E-4 2 0.0 0.0 0.0 0.0 1.3871183863929234E-4 3 0.0 0.0 0.0 0.0 1.3871183863929234E-4 4 0.0 0.0 0.0 0.0 1.3871183863929234E-4 5 0.0 0.0 0.0 0.0 1.3871183863929234E-4 6 0.0 0.0 0.0 0.0 1.3871183863929234E-4 7 0.0 0.0 0.0 0.0 1.3871183863929234E-4 8 0.0 0.0 0.0 0.0 1.3871183863929234E-4 9 0.0 0.0 0.0 1.3871183863929234E-4 1.3871183863929234E-4 10-11 0.0 0.0 0.0 1.3871183863929234E-4 1.3871183863929234E-4 12-13 0.0 0.0 0.0 1.3871183863929234E-4 1.3871183863929234E-4 14-15 0.0 0.0 0.0 1.3871183863929234E-4 1.3871183863929234E-4 16-17 0.0 0.0 0.0 1.3871183863929234E-4 1.3871183863929234E-4 18-19 0.0 0.0 0.0 1.3871183863929234E-4 1.3871183863929234E-4 20-21 0.0 0.0 0.0 1.3871183863929234E-4 1.3871183863929234E-4 22-23 0.0 0.0 0.0 1.3871183863929234E-4 1.3871183863929234E-4 24-25 0.0 0.0 0.0 2.080677579589385E-4 1.3871183863929234E-4 26-27 0.0 0.0 0.0 5.548473545571693E-4 1.3871183863929234E-4 28-29 0.0 0.0 0.0 7.629151125161078E-4 1.3871183863929234E-4 30-31 0.0 0.0 0.0 0.0015951861443518618 1.3871183863929234E-4 32-33 0.0 0.0 0.0 0.0037452196432608935 1.3871183863929234E-4 34-35 0.0 0.0 0.0 0.006242032738768156 1.3871183863929234E-4 36-37 0.0 0.0 0.0 0.01359376018665065 1.3871183863929234E-4 38-39 0.0 0.0 0.0 0.023997148084597575 1.3871183863929234E-4 40-41 0.0 0.0 0.0 0.03682799315873212 1.3871183863929234E-4 42-43 0.0 0.0 0.0 0.05333470195680791 1.3871183863929234E-4 44-45 0.0 0.0 0.0 0.07226886793107132 1.3871183863929234E-4 46-47 0.0 0.0 0.0 0.09411598251675986 1.3871183863929234E-4 48-49 0.0 0.0 0.0 0.11797441876271814 1.3871183863929234E-4 50-51 0.0 0.0 0.0 0.14204092276663538 1.3871183863929234E-4 52-53 0.0 0.0 0.0 0.17117040888088675 1.3871183863929234E-4 54-55 0.0 0.0 0.0 0.2047386738315955 1.3871183863929234E-4 56-57 0.0 0.0 0.0 0.24607480174610463 1.3871183863929234E-4 58-59 0.0 0.0 0.0 0.2919190644163907 1.3871183863929234E-4 60-61 0.0 0.0 0.0 0.346363461082313 1.3871183863929234E-4 62-63 0.0 0.0 0.0 0.3998368748777602 1.3871183863929234E-4 64-65 0.0 6.935591931964617E-5 0.0 0.45996845692789345 1.3871183863929234E-4 66-67 0.0 1.3871183863929234E-4 0.0 0.52731305458727 1.3871183863929234E-4 68-69 0.0 1.3871183863929234E-4 0.0 0.6097772426583292 1.3871183863929234E-4 70-71 0.0 1.3871183863929234E-4 0.0 0.6966802095658458 1.3871183863929234E-4 72-73 0.0 1.3871183863929234E-4 0.0 0.7917671749530807 1.3871183863929234E-4 74-75 0.0 2.7742367727858467E-4 0.0 0.8821379378265797 1.3871183863929234E-4 76-77 0.0 2.7742367727858467E-4 0.0 0.9850621220969347 1.3871183863929234E-4 78-79 0.0 2.7742367727858467E-4 0.0 1.1069204723415529 1.3871183863929234E-4 80-81 0.0 2.7742367727858467E-4 0.0 1.2287094666668517 1.3871183863929234E-4 82-83 0.0 2.7742367727858467E-4 0.0 1.363121238308326 1.3871183863929234E-4 84-85 0.0 2.7742367727858467E-4 0.0 1.496770094837284 1.3871183863929234E-4 86-87 0.0 2.7742367727858467E-4 0.0 1.634580306525421 1.3871183863929234E-4 88-89 0.0 2.7742367727858467E-4 0.0 1.7897294980434695 1.3871183863929234E-4 90-91 0.0 2.7742367727858467E-4 0.0 1.9621483134721098 1.3871183863929234E-4 92-93 0.0 2.7742367727858467E-4 0.0 2.1333880782723162 1.3871183863929234E-4 94-95 0.0 2.7742367727858467E-4 0.0 2.3109392317306106 1.3871183863929234E-4 96-97 0.0 2.7742367727858467E-4 0.0 2.487796825995708 1.3871183863929234E-4 98-99 0.0 2.7742367727858467E-4 0.0 2.6919806524727465 1.3871183863929234E-4 100-101 0.0 2.7742367727858467E-4 0.0 2.910382442410312 1.3871183863929234E-4 102-103 0.0 2.7742367727858467E-4 0.0 3.1424473484538487 1.3871183863929234E-4 104-105 0.0 2.7742367727858467E-4 0.0 3.3747203222553432 1.3871183863929234E-4 106-107 0.0 2.7742367727858467E-4 0.0 3.607132007895478 1.3871183863929234E-4 108-109 0.0 2.7742367727858467E-4 0.0 3.8551487753825326 1.3871183863929234E-4 110-111 0.0 2.7742367727858467E-4 0.0 4.123902962746161 1.3871183863929234E-4 112-113 0.0 2.7742367727858467E-4 0.0 4.430594837977637 1.3871183863929234E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGAA 35 8.885454E-7 85.11143 2 ACGTCGA 40 1.9632444E-6 74.48802 1 GTCGGAC 25 0.0020458763 71.4936 3 GTACACG 35 0.007748426 51.0775 1 CCGTATA 35 0.0077548125 51.066853 2 TCGAATA 90 9.6177246E-8 46.338444 4 GTCGAAT 80 2.272116E-6 44.6835 3 TACCTGG 805 0.0 42.18566 2 TTGGACG 100 2.20125E-7 41.704597 4 ACCTGGG 775 0.0 41.51241 3 GTACCTG 870 0.0 40.41189 1 TACGACG 60 0.0012997886 39.718666 4 TCCCGTC 75 8.122662E-5 39.718666 3 CGACGTG 65 0.0019391645 36.612526 6 ACGTTGA 90 2.370879E-4 33.105785 1 TAGGACC 1535 0.0 32.21481 4 ACGACGT 75 0.0038845332 31.774933 5 TACATGG 2965 0.0 30.944391 2 GTACATG 3045 0.0 30.920477 1 ACATGGG 3005 0.0 29.937695 3 >>END_MODULE