FastQCFastQC Report
Fri 27 May 2016
SRR939983_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939983_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450630
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA9130.2026052415507179No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA8890.19727936444533212No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC8810.19550407207687015No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6600.1464616203981093No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA5940.1318154583582984No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA5910.13114972372012515No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT5570.12360473115416194No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA5190.11517209240396778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGAC53.324351E-44947.8467108-109
CGTCGCG155.967522E-73298.564108-109
CGCGGCT108.251682E-43137.6587110-111
CGTTAGT108.251682E-43137.6587110-111
CGCCTCG108.251682E-43137.6587110-111
CGCCGTC108.251682E-43137.6587110-111
CGCCGAC108.251682E-43137.6587110-111
CGACGCC100.00132956362473.9233108-109
CCGCTCG100.00132956362473.9233108-109
CGTCGCT100.00132956362473.9233108-109
CCGCGCA100.00132956362473.9233108-109
CCGTTCG100.00132956362473.9233108-109
CCGCTCC1300.02283.6213108-109
CCGCGCC700.02120.5054108-109
TAGCTCG150.00185643472091.7722110-111
CGCTTTC150.00185643472091.7722110-111
TGCGTCG150.00185643472091.7722110-111
CCGCACG252.7619171E-61979.1384108-109
GGTCGCC457.179551E-91649.2821108-109
CGTGGCG150.00299112031649.282108-109
Produced by FastQC (version 0.11.5)