##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939983_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 450630 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.89531322814726 34.0 31.0 34.0 30.0 34.0 2 32.01985442602579 34.0 31.0 34.0 30.0 34.0 3 32.07477975279054 34.0 31.0 34.0 30.0 34.0 4 35.27297783103655 37.0 35.0 37.0 33.0 37.0 5 35.32111488360739 37.0 35.0 37.0 33.0 37.0 6 35.31346559261478 37.0 37.0 37.0 33.0 37.0 7 35.30430730310898 37.0 37.0 37.0 33.0 37.0 8 35.30264740474447 37.0 37.0 37.0 33.0 37.0 9 36.936424561169915 39.0 39.0 39.0 34.0 39.0 10-11 36.90712890841711 39.0 39.0 39.0 34.0 39.0 12-13 36.861006812684465 39.0 38.0 39.0 34.0 39.0 14-15 38.389893038634796 41.0 39.0 41.0 34.0 41.0 16-17 38.39081730022413 41.0 39.0 41.0 34.0 41.0 18-19 38.40819297428045 41.0 39.0 41.0 34.0 41.0 20-21 38.47911368528504 41.0 39.5 41.0 34.0 41.0 22-23 38.47676808024322 41.0 39.0 41.0 34.0 41.0 24-25 38.50881321705168 41.0 39.0 41.0 34.0 41.0 26-27 38.49423584759115 41.0 39.0 41.0 34.0 41.0 28-29 38.44030912278366 41.0 39.0 41.0 34.0 41.0 30-31 38.342965403989965 41.0 39.0 41.0 33.0 41.0 32-33 38.25443046401705 41.0 39.0 41.0 33.0 41.0 34-35 38.21243814215654 40.0 38.5 41.0 33.0 41.0 36-37 38.34858975212481 41.0 39.0 41.0 33.0 41.0 38-39 38.286538845616136 41.0 39.0 41.0 33.0 41.0 40-41 38.185514723831076 41.0 39.0 41.0 33.0 41.0 42-43 38.13573330670395 41.0 39.0 41.0 33.0 41.0 44-45 38.06923085458136 41.0 38.0 41.0 33.0 41.0 46-47 37.94791736014025 41.0 38.0 41.0 33.0 41.0 48-49 37.85803208840956 40.0 38.0 41.0 33.0 41.0 50-51 37.76451079599671 40.0 38.0 41.0 32.0 41.0 52-53 37.675461021236934 40.0 38.0 41.0 32.0 41.0 54-55 37.540968200075454 40.0 38.0 41.0 31.5 41.0 56-57 37.32962075316779 40.0 37.5 41.0 31.0 41.0 58-59 37.1608581319486 40.0 37.0 41.0 31.0 41.0 60-61 36.963386814015934 40.0 37.0 41.0 31.0 41.0 62-63 36.69198677407185 40.0 36.0 41.0 30.5 41.0 64-65 36.38216496904334 39.5 36.0 41.0 30.0 41.0 66-67 36.04047777555866 39.0 35.0 41.0 29.5 41.0 68-69 35.59374542307437 39.0 35.0 40.5 29.0 41.0 70-71 35.16689967379003 38.0 35.0 40.0 28.5 41.0 72-73 34.751091804806606 37.0 34.5 40.0 28.0 41.0 74-75 34.2882409071744 37.0 34.0 39.0 28.0 41.0 76-77 33.76357765794555 36.0 34.0 39.0 26.0 40.5 78-79 33.2582828484566 36.0 34.0 38.5 26.0 40.0 80-81 32.787076981115334 35.0 33.5 37.0 25.0 39.0 82-83 32.54462086412356 35.0 34.0 37.0 25.0 39.0 84-85 32.01956483145818 35.0 34.0 37.0 20.5 39.0 86-87 31.289490269178707 35.0 34.0 36.0 2.0 37.0 88-89 15.740840600936467 18.0 16.0 18.5 2.0 19.5 90-91 2.7243914075849367 2.0 2.0 2.0 2.0 2.0 92-93 2.2830448483234584 2.0 2.0 2.0 2.0 2.0 94-95 2.0950092093291612 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10479577733526568 1101 2 0.12671252623718487 1101 3 0.09877477773751053 1101 4 0.00988546507100807 1101 5 0.03328159859223234 1101 6 -0.028689721823553782 1101 7 -0.004719130970222807 1101 8 0.0122394218481503 1101 9 0.09535413932485426 1101 10-11 0.04525171475718537 1101 12-13 0.21883242604095443 1101 14-15 0.046523294313828956 1101 16-17 0.09481145869710872 1101 18-19 0.1683935839640398 1101 20-21 0.15490362744828445 1101 22-23 0.1269554701709268 1101 24-25 0.08062713405639954 1101 26-27 0.17807572296391072 1101 28-29 0.21880547104574077 1101 30-31 0.23137936846205776 1101 32-33 0.1779661157177017 1101 34-35 0.14719535764685077 1101 36-37 0.06005361516690755 1101 38-39 -0.005655199624570173 1101 40-41 0.03268889413143228 1101 42-43 0.1484981740601512 1101 44-45 0.12494978160887626 1101 46-47 0.03456020748392774 1101 48-49 0.11153851185344621 1101 50-51 -0.039166561245124853 1101 52-53 0.03548169825072023 1101 54-55 0.04711921877020586 1101 56-57 0.08331185546332165 1101 58-59 0.15582394877414885 1101 60-61 -0.008711712366270774 1101 62-63 0.14484040416395771 1101 64-65 0.2172364294339033 1101 66-67 0.02303188957264979 1101 68-69 0.1173043631160553 1101 70-71 0.42096373494739225 1101 72-73 0.41348460061139036 1101 74-75 0.14867380230111849 1101 76-77 0.17240720470880433 1101 78-79 0.07289281505471479 1101 80-81 0.07988865074605656 1101 82-83 0.18024439600810638 1101 84-85 0.23377489138638197 1101 86-87 0.11779898147719692 1101 88-89 0.2379027550976449 1101 90-91 -0.11592147392839847 1101 92-93 -0.11941066228394037 1101 94-95 -0.0072240650851256305 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.0668913994397009 1102 2 0.02897557380791227 1102 3 -0.02540845568349681 1102 4 0.003986854942702678 1102 5 0.04038983768754889 1102 6 -0.008403736696912745 1102 7 -0.10153484279548053 1102 8 -0.1055031381326259 1102 9 -2.7641473288042562E-5 1102 10-11 -0.20648729377430897 1102 12-13 -0.1671273331013623 1102 14-15 -0.15179176997273913 1102 16-17 -0.05533340305004231 1102 18-19 -0.12339936387238026 1102 20-21 -0.09623190173100937 1102 22-23 -0.1503312759805553 1102 24-25 -0.0934392753749691 1102 26-27 -0.06687414285539006 1102 28-29 -0.14839969858269342 1102 30-31 -0.2474347679082527 1102 32-33 -0.16736025713106528 1102 34-35 -0.2723402836834765 1102 36-37 -0.2368488801935058 1102 38-39 -0.21903455048372678 1102 40-41 -0.33276191295544066 1102 42-43 -0.4133411028327316 1102 44-45 -0.21809351669951127 1102 46-47 -0.19045881813723753 1102 48-49 -0.1907258708485955 1102 50-51 -0.5786584102684742 1102 52-53 -0.43871688918035545 1102 54-55 -0.26317330849985154 1102 56-57 -0.6073035065575354 1102 58-59 -0.5323988579388939 1102 60-61 -0.5194368221808148 1102 62-63 -0.515181759015789 1102 64-65 -0.4700531204933327 1102 66-67 -0.6543029213060052 1102 68-69 -0.6575657953640288 1102 70-71 -0.7228939934601968 1102 72-73 -0.8190645655990991 1102 74-75 -0.7626641327847921 1102 76-77 -0.8616680165892134 1102 78-79 -0.8160976921616765 1102 80-81 -0.9424707933052616 1102 82-83 -0.864897728719864 1102 84-85 -0.679194574160551 1102 86-87 -0.234666338487024 1102 88-89 0.5065111477330593 1102 90-91 0.16410095627761256 1102 92-93 -0.02754962275070172 1102 94-95 0.0906757733639858 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.13118415182648135 1103 2 0.032615713958556114 1103 3 0.04641350264896005 1103 4 -0.17948879703052256 1103 5 -0.06314697283633564 1103 6 0.019214646535360203 1103 7 -0.02561641550386895 1103 8 -0.03619623340568978 1103 9 -0.013116935055307977 1103 10-11 -0.01783860401495474 1103 12-13 0.11412463383316407 1103 14-15 -0.03433561949962893 1103 16-17 -0.009645448032522097 1103 18-19 -0.03578292134882588 1103 20-21 0.05031389899492211 1103 22-23 0.10312134974589782 1103 24-25 0.09057400536691063 1103 26-27 0.05720795436887016 1103 28-29 0.10322105546131866 1103 30-31 0.14942836492014067 1103 32-33 0.26756174735878346 1103 34-35 0.29172015103527826 1103 36-37 0.2801244534195675 1103 38-39 0.2368772679079001 1103 40-41 0.3436983392317856 1103 42-43 0.37373430156900866 1103 44-45 0.3601327804282448 1103 46-47 0.1967060162206451 1103 48-49 0.26905031822888503 1103 50-51 0.1587230491444842 1103 52-53 0.27286363449629647 1103 54-55 0.19869832148567212 1103 56-57 0.21951315416461625 1103 58-59 0.20787530650731867 1103 60-61 0.1696826205735178 1103 62-63 -0.1414642003224671 1103 64-65 0.07191470570308667 1103 66-67 0.12061158260689098 1103 68-69 0.10859716122703134 1103 70-71 0.1594230029521171 1103 72-73 0.18181399848624835 1103 74-75 0.24737510100241877 1103 76-77 0.3614125175777545 1103 78-79 0.09716967455884173 1103 80-81 0.23819738746389163 1103 82-83 0.28172019293844386 1103 84-85 0.3585535218468294 1103 86-87 0.11090700981249668 1103 88-89 0.7595231801271627 1103 90-91 -0.5003518163132861 1103 92-93 -0.3078404143500557 1103 94-95 -0.10722406508512572 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.10877325601303056 1104 2 0.016890225389616376 1104 3 0.04876817672887768 1104 4 0.0019352102720731068 1104 5 -0.13828389661543383 1104 6 -0.0712847103377996 1104 7 -0.14752007095385267 1104 8 0.010124458681779913 1104 9 0.09285895806115718 1104 10-11 0.1798528818155063 1104 12-13 0.21585537114696507 1104 14-15 0.21084352244468363 1104 16-17 0.1991892476232593 1104 18-19 0.18048663178174706 1104 20-21 0.1378439807342673 1104 22-23 0.02917472927371989 1104 24-25 0.15950786703240283 1104 26-27 0.18576589802266597 1104 28-29 0.21369364995948104 1104 30-31 0.12063028600226033 1104 32-33 0.21369209464728556 1104 34-35 0.27093522509859724 1104 36-37 0.2071399299558152 1104 38-39 0.22697622645733873 1104 40-41 0.19231976572861953 1104 42-43 0.2041407162405946 1104 44-45 0.15527613547574504 1104 46-47 0.24992735558415546 1104 48-49 0.14124701131216 1104 50-51 0.09914305246385169 1104 52-53 0.14683411289968262 1104 54-55 0.2011022673800298 1104 56-57 0.20593747265762374 1104 58-59 0.05995353977565543 1104 60-61 0.261722369961511 1104 62-63 0.09479155670005923 1104 64-65 0.07283012415046386 1104 66-67 0.21208081624864406 1104 68-69 0.2699130817282551 1104 70-71 0.1436923278763942 1104 72-73 0.3101748548822485 1104 74-75 0.04575483628311616 1104 76-77 0.1410352510742925 1104 78-79 0.3258038598623614 1104 80-81 0.30670854882648513 1104 82-83 0.3389273627654248 1104 84-85 0.19073631509192523 1104 86-87 0.03710561153026859 1104 88-89 0.5488498678164699 1104 90-91 -0.4913195147490357 1104 92-93 -0.3078404143500557 1104 94-95 -0.10722406508512572 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.12296612374863969 1105 2 -0.16239177076855782 1105 3 -0.1887384170823978 1105 4 -0.2771746003238036 1105 5 -0.2623570306912555 1105 6 -0.314892445749301 1105 7 -0.15585066723344454 1105 8 -0.30069536355902216 1105 9 -0.2625151225376072 1105 10-11 -0.31228928573199255 1105 12-13 -0.20725083649090692 1105 14-15 -0.10901853004703099 1105 16-17 -0.19048758097746088 1105 18-19 -0.1599257178047111 1105 20-21 -0.179960304994232 1105 22-23 -0.32344487997453797 1105 24-25 -0.1703344786724159 1105 26-27 -0.21562552056740714 1105 28-29 -0.18057147599476053 1105 30-31 -0.3604964843738614 1105 32-33 -0.22056636284875708 1105 34-35 -0.19165920325528418 1105 36-37 -0.2625384911125366 1105 38-39 -0.23557590197518152 1105 40-41 -0.14848821253419686 1105 42-43 -0.10326027706108931 1105 44-45 -0.12401780418963426 1105 46-47 -0.030983934016035164 1105 48-49 -0.1650815545712092 1105 50-51 -0.30462960287638907 1105 52-53 -0.31393644237387264 1105 54-55 -0.31187385555846703 1105 56-57 -0.19496625272132917 1105 58-59 -0.3258590665962089 1105 60-61 -0.32548265029268464 1105 62-63 -0.18954851476192403 1105 64-65 -0.26190159481881636 1105 66-67 -0.16494087116986833 1105 68-69 -0.10642185402562632 1105 70-71 -0.12640759946727087 1105 72-73 -0.1606780110618402 1105 74-75 -0.07405347042615062 1105 76-77 -0.1885657362556401 1105 78-79 -0.3224175528554838 1105 80-81 -0.5786113878729253 1105 82-83 -0.2928742157681157 1105 84-85 -0.3101992596395924 1105 86-87 -0.35049354446189085 1105 88-89 0.7244890942753184 1105 90-91 -0.7349297383912083 1105 92-93 -0.3078404143500557 1105 94-95 -0.10722406508512572 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.10952167451884165 1106 2 -0.0717691484908265 1106 3 -0.050884360777935456 1106 4 -0.14415853589381555 1106 5 -0.19313929234478877 1106 6 -0.23390991707307052 1106 7 -0.07875145250987714 1106 8 -0.1936637658732181 1106 9 -0.10344998909803849 1106 10-11 -0.32176787367002646 1106 12-13 -0.1917247587097819 1106 14-15 -0.1250614259512446 1106 16-17 -0.3021848978300383 1106 18-19 -0.41735497832411284 1106 20-21 -0.2714550578546806 1106 22-23 -0.32317211485402453 1106 24-25 -0.36751494868978796 1106 26-27 -0.4014395808915694 1106 28-29 -0.3291207962353724 1106 30-31 -0.1767568745715451 1106 32-33 -0.22787359686687836 1106 34-35 -0.09735784086529264 1106 36-37 0.05215349964215932 1106 38-39 0.0065497996772023725 1106 40-41 0.03237659645844104 1106 42-43 -0.1651743704041806 1106 44-45 -0.0969961121845273 1106 46-47 -0.07156133470800086 1106 48-49 -0.217150980472411 1106 50-51 -0.15124587977605586 1106 52-53 -0.24269808872671206 1106 54-55 -0.10004661637217538 1106 56-57 -0.4763306525662898 1106 58-59 -0.46546815111032913 1106 60-61 -0.27131723978117606 1106 62-63 -0.17274474773204673 1106 64-65 -0.33706274158080873 1106 66-67 -0.24396738681078745 1106 68-69 -0.28050701416746193 1106 70-71 -0.5164078865884534 1106 72-73 -0.6392480040273654 1106 74-75 -0.729583424343204 1106 76-77 -0.8941121996405101 1106 78-79 -0.520821876900456 1106 80-81 -0.26163678374125254 1106 82-83 -0.443562588795956 1106 84-85 -0.5998758176308918 1106 86-87 -0.3145136716565702 1106 88-89 0.30125094161298627 1106 90-91 -0.43846276450487975 1106 92-93 -0.28556698885082366 1106 94-95 -0.10722406508512572 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.028836786548080084 1107 2 0.02865989965791016 1107 3 0.08490805407454616 1107 4 -0.059414905404906904 1107 5 -0.07074138991351475 1107 6 -0.08167727763242283 1107 7 -0.037819729431319615 1107 8 -0.12100366735105439 1107 9 0.025284318863185717 1107 10-11 0.048309328512289085 1107 12-13 0.004882582780759037 1107 14-15 0.07824648230661069 1107 16-17 -0.09463947699207154 1107 18-19 -0.0902686654897451 1107 20-21 -0.15392367377713612 1107 22-23 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0.03406242637154833 2216 18-19 0.19365773293359467 2216 20-21 0.069455358800937 2216 22-23 -0.050102799522555586 2216 24-25 0.08126527612147072 2216 26-27 -0.045064772903856465 2216 28-29 -0.029525944374285018 2216 30-31 5.793820356814194E-6 2216 32-33 -0.21089255150690178 2216 34-35 -0.24215499280821007 2216 36-37 -0.06304503531954708 2216 38-39 -0.241443461995118 2216 40-41 -0.2279973701221465 2216 42-43 -0.17268605023109274 2216 44-45 -0.226666496244448 2216 46-47 -0.3916036171839252 2216 48-49 -0.2467416429744631 2216 50-51 -0.33191684893212425 2216 52-53 -0.3650309076704019 2216 54-55 -0.3206075308898022 2216 56-57 -0.2664004575401364 2216 58-59 -0.506975507235893 2216 60-61 -0.3897452932847685 2216 62-63 -0.48027728597099895 2216 64-65 -0.5508146219313019 2216 66-67 -0.5907561635709797 2216 68-69 -0.4870998632674528 2216 70-71 -0.3985048287098891 2216 72-73 -0.7154200965326538 2216 74-75 -0.6991973139244223 2216 76-77 -0.7375415346990835 2216 78-79 -0.4991335475355214 2216 80-81 -0.6135102403513386 2216 82-83 -0.7015528631194243 2216 84-85 -0.8885182355097356 2216 86-87 -1.30209849733858 2216 88-89 -1.4506629944577476 2216 90-91 -0.7349297383912083 2216 92-93 -0.3078404143500557 2216 94-95 -0.10722406508512572 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5314.0 3 766.0 4 533.0 5 495.0 6 511.0 7 521.0 8 624.0 9 715.0 10 897.0 11 1257.0 12 1444.0 13 1698.0 14 1929.0 15 2222.0 16 2409.0 17 2935.0 18 3689.0 19 4472.0 20 4609.0 21 6624.0 22 7688.0 23 9566.0 24 14686.0 25 25381.0 26 42896.0 27 114389.0 28 185630.0 29 6472.0 30 258.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.79435457026829 21.420011983223485 15.949670461354104 23.83596298515412 2 13.389019202304365 20.75215867809677 45.63970325527104 20.219118864327832 3 18.155539619776253 27.459099194305747 30.458972645470105 23.926388540447892 4 11.318802578147029 19.394051078927443 40.003907628979505 29.28323871394602 5 11.078596802841918 38.01221136767318 34.03108348134991 16.87810834813499 6 25.965204269578145 36.99553957792424 22.938108869804495 14.10114728269312 7 26.04819918780374 32.54754454874288 23.847280474003064 17.556975789450323 8 23.90630894525442 39.32427934225417 21.471939285001 15.297472427490403 9 24.671903779153627 20.699909016266115 23.648669640281383 30.97951756429887 10-11 22.75592482005568 26.723964800039056 31.83042237955908 18.689688000346187 12-13 25.96134215081443 25.12172118414629 30.07822555590076 18.83871110913852 14-15 20.16479169694838 31.541543551853575 25.9515855042779 22.34207924692014 16-17 19.294804011748088 31.473515206052017 30.86758102330866 18.364099758891232 18-19 20.809457018194085 28.40290199873054 32.37994522688233 18.407695756193046 20-21 21.356464426789756 29.273001642181885 29.21585815099197 20.154675780036392 22-23 19.854225334922234 32.01971919126018 29.59321123678785 18.53284423702974 24-25 20.608037636198212 29.635843152919243 31.697623327341724 18.05849588354082 26-27 18.409559949404166 31.886802920355944 30.26540620908506 19.43823092115483 28-29 18.53961258424373 32.06498944803574 28.164535192076816 21.230862775643715 30-31 19.539755453476243 29.695981181900894 30.41175687371014 20.352506490912724 32-33 18.494235184073553 30.872250880173276 31.899720719051356 18.733793216701823 34-35 18.867072469814413 31.324881443493428 31.011097821045695 18.796948265646463 36-37 18.2701290862328 30.986243672733004 30.199232628170847 20.544394612863353 38-39 19.996671326809135 31.745445265517162 28.83740541020349 19.420477997470208 40-41 22.119588132170517 28.248674078512302 31.342564853649336 18.289172935667843 42-43 18.77105385793223 31.646139848656325 31.25690699687105 18.3258992965404 44-45 18.332617666215075 31.909987939067324 29.76664813699089 19.99074625772671 46-47 20.780573863258105 29.500255198277962 29.655926147837476 20.063244790626456 48-49 19.42307308229164 30.396179562345075 29.74364721311766 20.43710014224562 50-51 17.85613236594586 32.11263354929049 30.17112727861802 19.860106806145623 52-53 17.84634844550962 30.330426292079977 31.46561480593835 20.35761045647205 54-55 18.858704516781895 29.50072067453805 30.684202525634152 20.956372283045894 56-57 19.728602179171382 30.725650755608815 27.72740385682267 21.818343208397135 58-59 19.30563877238533 31.84408494773983 27.5376694849433 21.31260679493154 60-61 18.83174666577902 28.54947517919357 32.48019439451434 20.13858376051306 62-63 19.82835141912434 28.474136209306973 31.248696269666908 20.44881610190178 64-65 20.8312806515323 30.1375851585558 28.36018463040632 20.67094955950558 66-67 20.17076093469143 32.37079200230788 27.673368395357613 19.785078667643077 68-69 21.31920049530767 28.608678092177826 31.06812921494178 19.003992197572725 70-71 22.23609169385083 28.55469010052593 29.812928566673325 19.396289638949916 72-73 20.47622217783991 31.452854892040033 28.323458269533763 19.74746466058629 74-75 20.129041564032576 31.179016044204783 30.487428710915832 18.204513680846816 76-77 20.325877105385793 29.62807624880723 31.271220291591774 18.77482635421521 78-79 19.665755716737845 29.218857662131892 30.498181983607342 20.617204637522928 80-81 20.079111466169586 30.525597496837758 29.96282981603533 19.432461220957325 82-83 19.689653259361997 29.926102635228848 31.50502080443828 18.879223300970875 84-85 19.536426780285378 30.199498479905913 31.023234138872247 19.24084060093647 86-87 17.87082621822334 32.84645453930148 28.28424046131433 20.998478781160852 88-89 28.483341305759307 19.469215637350654 35.66045460748783 16.386988449402207 90-91 30.058886791459532 15.900035347116201 18.16616152880038 35.87491633262388 92-93 22.109782218310187 18.04742701996565 37.79106877345903 22.051721988265136 94-95 15.585928368171157 7.174871625379884 28.322020781775237 48.917179224673724 96-97 27.10665122861186 7.441142416055577 15.513744157125092 49.938462198207475 98-99 57.59233056169415 13.931859012066964 18.680742142164423 9.795068284074468 100-101 30.393793082922965 7.151965531372266 13.322481759511726 49.131759626193045 102-103 22.424645955224374 2.0461167226243937 37.95799156620247 37.57124575594876 104-105 39.7966086706608 4.3446156567108085 43.74991709592554 12.108858576702849 106-107 5.600185009372185 1.949901409479296 6.522797536454148 85.92711604469437 108-109 18.462518915734844 11.467378609223768 23.481917065293562 46.58818540974782 110-111 40.22408197498451 2.8693067862742954 26.347956095248513 30.55865514349269 112-113 26.8301937430302 2.718510395121026 39.73814382423847 30.713152037610303 114-115 27.884501564217466 3.872495138243003 24.491417941997128 43.7515853555424 116-117 19.670208430657297 4.27496546034649 20.46967302461344 55.585153084382775 118-119 20.50751027680958 4.361196642648369 16.19476703293268 58.93652604760937 120-121 25.05402255936825 4.262973899467128 31.180916177352387 39.50208736381224 122-123 34.071536940830306 1.7093466967035997 34.52209093834766 29.697025424118433 124-125 38.64930586258732 2.1489521619948713 20.484790874524712 38.71695110089309 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12.0 1 26.5 2 55.5 3 76.0 4 107.5 5 114.0 6 89.5 7 74.0 8 59.5 9 58.5 10 57.0 11 49.5 12 51.0 13 49.5 14 59.5 15 83.0 16 112.5 17 160.5 18 205.5 19 276.5 20 401.5 21 611.5 22 867.0 23 1190.0 24 1643.0 25 2221.0 26 2998.0 27 3957.5 28 5205.5 29 6927.0 30 9468.0 31 12748.5 32 16948.5 33 21775.5 34 26012.5 35 28969.5 36 30111.5 37 29162.5 38 27201.5 39 24935.0 40 22406.5 41 19965.0 42 17553.5 43 15265.5 44 13424.0 45 12215.5 46 11331.5 47 10468.5 48 9752.5 49 9086.5 50 8360.0 51 7698.0 52 6875.5 53 5953.0 54 5110.0 55 4271.5 56 3467.0 57 2828.5 58 2275.5 59 1780.5 60 1360.0 61 1003.5 62 737.5 63 546.0 64 419.0 65 308.0 66 217.5 67 167.0 68 128.5 69 98.5 70 70.0 71 54.5 72 49.5 73 41.0 74 36.0 75 29.5 76 25.5 77 25.5 78 22.0 79 16.5 80 10.0 81 8.0 82 7.0 83 6.5 84 4.0 85 1.5 86 3.5 87 2.5 88 2.0 89 1.5 90 0.5 91 1.0 92 1.5 93 2.5 94 2.0 95 0.5 96 1.0 97 1.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 0.0015533808224041897 3 0.04682333621818343 4 0.05081774404722278 5 0.05103965559328052 6 0.0 7 0.0 8 0.0 9 0.0 10-11 9.986019572598363E-4 12-13 0.0022191154605774137 14-15 1.1095577302887069E-4 16-17 7.766904112020949E-4 18-19 0.009764108026540621 20-21 0.0022191154605774137 22-23 6.657346381732242E-4 24-25 0.0 26-27 0.0 28-29 6.657346381732242E-4 30-31 0.0 32-33 0.001886248141490802 34-35 2.2191154605774138E-4 36-37 2.2191154605774138E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 3.328673190866121E-4 46-47 0.0 48-49 2.2191154605774138E-4 50-51 1.1095577302887069E-4 52-53 0.0 54-55 0.0023300712336062845 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.2191154605774138E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.328673190866121E-4 80-81 0.0 82-83 0.0011095577302887068 84-85 0.0 86-87 9.986019572598363E-4 88-89 19.51079599671571 90-91 26.232718638350754 92-93 40.37536338015667 94-95 44.94330159998225 96-97 48.252446574795286 98-99 93.02099283225706 100-101 51.53418547367019 102-103 53.00645762599028 104-105 74.90568759292546 106-107 81.76819119898808 108-109 12.59969376206644 110-111 39.14519672458558 112-113 52.042473869915455 114-115 86.87726072387547 116-117 66.51055189401505 118-119 71.60464238954353 120-121 80.38546035550229 122-123 75.86600980849033 124-125 49.808046512660056 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 450630.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.69650584843457 #Duplication Level Percentage of deduplicated Percentage of total 1 92.04419370814911 73.3560062217595 2 5.429285372686123 8.653901469141998 3 1.06608896241049 2.5489069568309746 4 0.45458234592827146 1.4491449836347035 5 0.23782035888313047 0.9476725811303106 6 0.1547133889560421 0.7398069904659806 7 0.10590714020979335 0.5908300313384542 8 0.0772280951861278 0.4923847471731757 9 0.05396004147264856 0.38703840847260373 >10 0.3215307540501414 4.8134513500642555 >50 0.030414243851844464 1.6845055259731796 >100 0.022043350219379126 3.090150246147245 >500 0.0022322379968991455 1.2462004878676693 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 913 0.2026052415507179 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 889 0.19727936444533212 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 881 0.19550407207687015 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 660 0.1464616203981093 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 594 0.1318154583582984 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA 591 0.13114972372012515 No Hit ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT 557 0.12360473115416194 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 519 0.11517209240396778 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.2191154605774138E-4 0.0 24-25 0.0 0.0 0.0 2.2191154605774138E-4 0.0 26-27 0.0 0.0 0.0 2.2191154605774138E-4 0.0 28-29 0.0 0.0 0.0 4.4382309211548276E-4 0.0 30-31 0.0 0.0 0.0 0.0018862481414908018 0.0 32-33 0.0 0.0 0.0 0.004216319375097087 0.0 34-35 0.0 0.0 0.0 0.006990213700818854 2.2191154605774138E-4 36-37 0.0 0.0 0.0 0.013758515855579967 2.2191154605774138E-4 38-39 0.0 0.0 0.0 0.027184164392073322 2.2191154605774138E-4 40-41 0.0 0.0 0.0 0.042163193750970863 2.2191154605774138E-4 42-43 0.0 0.0 0.0 0.0628009675343408 2.2191154605774138E-4 44-45 0.0 0.0 0.0 0.08343874131771076 2.2191154605774138E-4 46-47 0.0 0.0 0.0 0.10352173623593636 2.2191154605774138E-4 48-49 0.0 0.0 0.0 0.13048398908195194 2.2191154605774138E-4 50-51 0.0 0.0 0.0 0.16598983645119056 2.2191154605774138E-4 52-53 0.0 0.0 0.0 0.19827796640259193 2.2191154605774138E-4 54-55 0.0 0.0 0.0 0.23644675232452345 2.2191154605774138E-4 56-57 0.0 0.0 0.0 0.28049619421698513 2.2191154605774138E-4 58-59 0.0 0.0 0.0 0.33075915939906353 2.2191154605774138E-4 60-61 0.0 0.0 0.0 0.3952244635288374 2.2191154605774138E-4 62-63 0.0 0.0 0.0 0.4610212369349577 2.2191154605774138E-4 64-65 0.0 0.0 0.0 0.5253755852917028 2.2191154605774138E-4 66-67 0.0 0.0 0.0 0.5997159532210461 2.2191154605774138E-4 68-69 0.0 0.0 0.0 0.6942502718416439 2.2191154605774138E-4 70-71 0.0 0.0 0.0 0.7920023078800791 2.2191154605774138E-4 72-73 0.0 0.0 0.0 0.8840955994940416 2.2191154605774138E-4 74-75 0.0 0.0 0.0 0.9903912300556998 2.2191154605774138E-4 76-77 0.0 0.0 0.0 1.1006812684463974 2.2191154605774138E-4 78-79 0.0 0.0 0.0 1.1330803541708274 2.2191154605774138E-4 80-81 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 82-83 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 84-85 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 86-87 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 88-89 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 90-91 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 92-93 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 94-95 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 96-97 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 98-99 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 100-101 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 102-103 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 104-105 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 106-107 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 108-109 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 110-111 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 112-113 0.0 0.0 0.0 1.133524177262943 2.2191154605774138E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGGAC 5 3.324351E-4 4947.8467 108-109 CGTCGCG 15 5.967522E-7 3298.564 108-109 CGCGGCT 10 8.251682E-4 3137.6587 110-111 CGTTAGT 10 8.251682E-4 3137.6587 110-111 CGCCTCG 10 8.251682E-4 3137.6587 110-111 CGCCGTC 10 8.251682E-4 3137.6587 110-111 CGCCGAC 10 8.251682E-4 3137.6587 110-111 CGACGCC 10 0.0013295636 2473.9233 108-109 CCGCTCG 10 0.0013295636 2473.9233 108-109 CGTCGCT 10 0.0013295636 2473.9233 108-109 CCGCGCA 10 0.0013295636 2473.9233 108-109 CCGTTCG 10 0.0013295636 2473.9233 108-109 CCGCTCC 130 0.0 2283.6213 108-109 CCGCGCC 70 0.0 2120.5054 108-109 TAGCTCG 15 0.0018564347 2091.7722 110-111 CGCTTTC 15 0.0018564347 2091.7722 110-111 TGCGTCG 15 0.0018564347 2091.7722 110-111 CCGCACG 25 2.7619171E-6 1979.1384 108-109 GGTCGCC 45 7.179551E-9 1649.2821 108-109 CGTGGCG 15 0.0029911203 1649.282 108-109 >>END_MODULE