FastQCFastQC Report
Fri 27 May 2016
SRR939983_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939983_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450630
Sequences flagged as poor quality0
Sequence length125
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10880.24143976211082266No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA9910.2199143421432217No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA8970.199054656813794No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC8370.18573996405032955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7980.1770854137540776No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA6460.14335485875330095No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA5910.13114972372012515No Hit
ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT5730.1271553158910858No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA5690.12626766970685485No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA5680.1260457581607971No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.10629563056165812No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTAC150.004234221859.507214118-119
GGTATCA8500.055.3497051
GTATCAA15050.054.607181
TACCTGG5350.054.544312
GTACCTG5750.051.7856371
ACCTGGG6550.041.823823
ATCAACG19550.041.428513
TATCAAC19650.041.2176742
TCAACGC19950.040.8963744
CAACGCA20300.040.1912655
TTCAACG758.137515E-539.702322
GTATAAC2000.038.7097631
AACGCAG21100.038.346856
CCTGGGG3550.035.228824
ACGCAGA23900.033.6054047
CGCAGAG24000.033.465388
CGGTAGG902.3781149E-433.0852661
TACATGG20100.032.8877452
GTATAGC2203.6379788E-1232.483721
GTACATG20750.032.144531
Produced by FastQC (version 0.11.5)