##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939983_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 450630 Sequences flagged as poor quality 0 Sequence length 125 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.84287331069836 34.0 31.0 34.0 31.0 34.0 2 32.9498790582074 34.0 33.0 34.0 31.0 34.0 3 32.97045247764241 34.0 33.0 34.0 31.0 34.0 4 36.36937842575949 37.0 37.0 37.0 35.0 37.0 5 36.27623327341722 37.0 37.0 37.0 35.0 37.0 6 36.412094179260144 37.0 37.0 37.0 35.0 37.0 7 36.41524088498325 37.0 37.0 37.0 35.0 37.0 8 36.44358342764574 37.0 37.0 37.0 35.0 37.0 9 38.285786565475 39.0 39.0 39.0 37.0 39.0 10-11 38.27440028404678 39.0 39.0 39.0 37.0 39.0 12-13 38.25961653684841 39.0 39.0 39.0 37.0 39.0 14-15 39.808453720347075 41.0 40.0 41.0 38.0 41.0 16-17 39.82520138472805 41.0 40.0 41.0 38.0 41.0 18-19 39.86440094978141 41.0 40.0 41.0 38.0 41.0 20-21 39.87212014291103 41.0 40.0 41.0 38.0 41.0 22-23 39.799745911279764 41.0 40.0 41.0 37.5 41.0 24-25 39.7653673745645 41.0 40.0 41.0 37.0 41.0 26-27 39.668360961320815 41.0 40.0 41.0 37.0 41.0 28-29 39.5809400173091 41.0 40.0 41.0 37.0 41.0 30-31 39.43105208263986 41.0 39.5 41.0 36.0 41.0 32-33 39.257324190577634 41.0 39.0 41.0 35.5 41.0 34-35 39.124649934536095 41.0 39.0 41.0 35.0 41.0 36-37 39.06945165656969 41.0 39.5 41.0 35.0 41.0 38-39 39.099522890175976 41.0 40.0 41.0 35.0 41.0 40-41 38.99348467700774 41.0 39.5 41.0 35.0 41.0 42-43 38.91155937243415 41.0 39.5 41.0 35.0 41.0 44-45 38.78268091338792 41.0 40.0 41.0 35.0 41.0 46-47 38.55442491622839 41.0 39.0 41.0 35.0 41.0 48-49 38.33761733572998 41.0 39.0 41.0 35.0 41.0 50-51 38.214937975722876 41.0 39.0 41.0 35.0 41.0 52-53 38.124299314293324 41.0 39.0 41.0 34.5 41.0 54-55 38.039735481437106 41.0 39.0 41.0 34.5 41.0 56-57 37.89478507866764 41.0 39.0 41.0 34.0 41.0 58-59 37.75682599915673 41.0 38.0 41.0 34.0 41.0 60-61 37.59505913942702 40.0 38.0 41.0 34.0 41.0 62-63 37.399120120719886 40.0 37.5 41.0 33.0 41.0 64-65 37.15470785344961 40.0 37.0 41.0 33.0 41.0 66-67 36.88486785167432 39.5 36.5 41.0 33.0 41.0 68-69 36.54906686194883 39.0 36.0 41.0 32.0 41.0 70-71 36.17390098306815 39.0 35.5 41.0 31.5 41.0 72-73 35.83321793932939 37.5 35.0 40.0 31.0 41.0 74-75 35.46866498013892 37.0 35.0 39.5 31.0 41.0 76-77 35.052545325433286 36.5 35.0 39.0 31.0 41.0 78-79 34.673388367396754 36.0 35.0 39.0 30.5 40.5 80-81 34.38910858131949 36.0 35.0 37.5 30.5 39.0 82-83 34.13287397643299 35.5 35.0 37.0 31.0 39.0 84-85 33.815054479284555 35.0 35.0 37.0 30.5 39.0 86-87 33.564677229656255 35.0 35.0 36.5 30.5 37.0 88-89 33.31703725894859 35.0 35.0 36.0 30.0 37.0 90-91 33.08727115371813 35.0 34.0 36.0 30.0 37.0 92-93 32.949980027960855 35.0 34.0 35.5 30.0 36.0 94-95 32.845880212147435 35.0 34.0 35.0 30.0 36.0 96-97 32.68248895990058 35.0 34.0 35.0 29.0 36.0 98-99 32.512870869671346 35.0 34.0 35.0 29.0 36.0 100-101 32.53393360406542 35.0 34.0 35.0 29.0 36.0 102-103 31.700644653041298 34.5 32.5 35.0 27.5 35.0 104-105 32.37984377427158 35.0 34.0 35.0 29.0 35.0 106-107 32.451350331757766 35.0 34.0 35.0 29.0 35.5 108-109 32.44389632292568 35.0 34.0 35.0 29.0 35.5 110-111 32.417764019261924 35.0 34.0 35.0 29.0 35.0 112-113 32.32381887579611 35.0 34.0 35.0 29.0 35.5 114-115 32.23871579788296 35.0 34.0 35.0 29.0 35.0 116-117 32.182030712557975 35.0 34.0 35.0 29.0 35.0 118-119 32.098594190355726 35.0 34.0 35.0 29.0 35.0 120-121 31.989276124536758 35.0 34.0 35.0 29.0 35.0 122-123 31.803522845793665 35.0 34.0 35.0 27.0 35.0 124-125 29.732910591838092 33.5 29.5 34.5 13.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09471597910565066 1101 2 0.11198818198926119 1101 3 0.07777846471529415 1101 4 -0.045745830193453685 1101 5 -0.10230512048499918 1101 6 -0.05680578840514272 1101 7 -0.0975422589086321 1101 8 0.0018670804768845528 1101 9 0.08348245734212867 1101 10-11 -0.02303893860824502 1101 12-13 -0.002879722942417118 1101 14-15 0.05841735837619666 1101 16-17 0.12011541765907197 1101 18-19 0.0595695171006696 1101 20-21 0.12366274887201456 1101 22-23 0.16535408474207003 1101 24-25 0.08847193508542972 1101 26-27 0.10865977660659354 1101 28-29 0.19985867808236435 1101 30-31 0.03857525955867658 1101 32-33 0.16573596122004375 1101 34-35 0.2647560463666636 1101 36-37 0.2654455277519219 1101 38-39 0.27452595281786785 1101 40-41 0.4047521557918543 1101 42-43 0.23356999127622657 1101 44-45 0.314584867787552 1101 46-47 0.494461797277566 1101 48-49 0.38416976005918 1101 50-51 0.30296951659178006 1101 52-53 0.25633918025276614 1101 54-55 0.30447984109790127 1101 56-57 0.21897511814051285 1101 58-59 0.3577283876580566 1101 60-61 0.3861520064897732 1101 62-63 0.24010728330036102 1101 64-65 0.3585892272104232 1101 66-67 0.3795750840097867 1101 68-69 0.21630323175510568 1101 70-71 0.27297138068943383 1101 72-73 0.32797271415858376 1101 74-75 0.3777884210003606 1101 76-77 0.3820337564132714 1101 78-79 0.2714222203557455 1101 80-81 0.254424253006988 1101 82-83 0.2496885599830776 1101 84-85 0.34834969518031045 1101 86-87 0.3471635146288605 1101 88-89 0.2907447227515121 1101 90-91 0.2246718291680594 1101 92-93 0.32191444260445223 1101 94-95 0.183407450068799 1101 96-97 0.27781000467109607 1101 98-99 0.33288140357233686 1101 100-101 0.3729007096280057 1101 102-103 0.360053310951848 1101 104-105 0.38220062106534414 1101 106-107 0.3278537574784295 1101 108-109 0.24237263258737585 1101 110-111 0.42238889211925823 1101 112-113 0.5929709034194701 1101 114-115 0.5226645842753967 1101 116-117 0.5149948263742488 1101 118-119 0.5936705008550476 1101 120-121 0.6096606547825871 1101 122-123 0.7781386260242869 1101 124-125 0.703634406346886 1102 1 0.06744639956429666 1102 2 0.12484549650614696 1102 3 0.16532302472277394 1102 4 0.06643724301179788 1102 5 0.017789940864282983 1102 6 0.013868052367094208 1102 7 0.07727182405642452 1102 8 -0.0067605380493915845 1102 9 0.11399287804200497 1102 10-11 0.1931308366891571 1102 12-13 0.1114008415510952 1102 14-15 0.08119069313367078 1102 16-17 0.2398833718373652 1102 18-19 0.1382354538955468 1102 20-21 0.13283243071022355 1102 22-23 0.16091877852969816 1102 24-25 0.1632966991009681 1102 26-27 0.14307011586345908 1102 28-29 0.07563138532812275 1102 30-31 0.05476773208835084 1102 32-33 0.0843356755019471 1102 34-35 0.09137989982265537 1102 36-37 0.15876853606978614 1102 38-39 0.1685672617806091 1102 40-41 0.11846261921593282 1102 42-43 0.06059972465050123 1102 44-45 0.15648662882575337 1102 46-47 0.09258593778678659 1102 48-49 0.03981402767289666 1102 50-51 0.05691149178504418 1102 52-53 0.07875603495043038 1102 54-55 0.04762060399193757 1102 56-57 0.24048274841594974 1102 58-59 0.1318201645843189 1102 60-61 0.18573945091493016 1102 62-63 0.22528866088885025 1102 64-65 0.22503337199206186 1102 66-67 0.2752799665849892 1102 68-69 0.07580869911781463 1102 70-71 0.09333213316943301 1102 72-73 -0.0492993455484978 1102 74-75 0.039476828055192925 1102 76-77 0.1872674711491129 1102 78-79 0.22797170699775648 1102 80-81 0.01578288262821559 1102 82-83 0.13101541347911905 1102 84-85 0.235493715794739 1102 86-87 0.23452249138422587 1102 88-89 0.09926686590429057 1102 90-91 0.06180717142896697 1102 92-93 0.09371112880853616 1102 94-95 0.15046575525311567 1102 96-97 0.08950976034206093 1102 98-99 0.15940779244267844 1102 100-101 0.13496428858099563 1102 102-103 0.11302331055130921 1102 104-105 0.08282145712457378 1102 106-107 -0.0025378365348487364 1102 108-109 0.07250654625778452 1102 110-111 0.00983865780371218 1102 112-113 0.03183924612096689 1102 114-115 0.16553832090739462 1102 116-117 0.12866924125295043 1102 118-119 0.035143684742685366 1102 120-121 -0.07873972439470478 1102 122-123 -0.002667526260786701 1102 124-125 0.22269874632472408 1103 1 0.055697244034845994 1103 2 0.17072489981688932 1103 3 0.15142073153938185 1103 4 -0.030456573995103042 1103 5 -0.0018033495286857715 1103 6 0.01441617145317764 1103 7 0.05543885089065981 1103 8 0.042481011999910834 1103 9 0.0964399543905401 1103 10-11 0.08994807437876773 1103 12-13 0.07917458638461738 1103 14-15 0.07749941268551908 1103 16-17 0.10306996311361871 1103 18-19 0.16272772253159928 1103 20-21 0.23363913612112697 1103 22-23 0.25821122759921167 1103 24-25 0.2474447804219082 1103 26-27 0.16012081556763036 1103 28-29 0.29084155883796825 1103 30-31 0.16279308718559804 1103 32-33 0.2541804712554594 1103 34-35 0.25895616443041547 1103 36-37 0.18597386305297903 1103 38-39 0.1196705809170453 1103 40-41 0.1630608925096837 1103 42-43 0.09149502079216632 1103 44-45 -0.027888567867698555 1103 46-47 -0.06802344475313049 1103 48-49 0.15872702098008062 1103 50-51 0.350637757441838 1103 52-53 0.08725712594343804 1103 54-55 0.08156071476969373 1103 56-57 0.13095858921489167 1103 58-59 0.14695507006656072 1103 60-61 0.14599557201515978 1103 62-63 0.05893845213153526 1103 64-65 -0.02213686487930744 1103 66-67 0.12213706748086395 1103 68-69 0.1432365257338546 1103 70-71 0.21184387183465248 1103 72-73 0.33790482749978423 1103 74-75 0.2345180550027237 1103 76-77 0.14720494885718693 1103 78-79 0.2642498472742787 1103 80-81 0.2866704159349638 1103 82-83 0.129263530467135 1103 84-85 0.09064603520392467 1103 86-87 0.135489960909851 1103 88-89 0.022740000201331156 1103 90-91 0.13574030614562105 1103 92-93 0.10219779561506925 1103 94-95 0.14980355396490097 1103 96-97 0.13163231872068337 1103 98-99 0.05247998680728472 1103 100-101 0.2187832361923583 1103 102-103 0.1877982932422846 1103 104-105 0.18189070370997484 1103 106-107 0.19303970789164993 1103 108-109 0.25865067272905407 1103 110-111 0.13956716838843874 1103 112-113 0.23991600377365785 1103 114-115 0.061315705881064275 1103 116-117 0.29183957253717097 1103 118-119 0.1350075728739384 1103 120-121 0.15791921440477807 1103 122-123 0.21395621752369465 1103 124-125 0.08388824341890455 1104 1 -0.009943185822834266 1104 2 0.06476456622077365 1104 3 0.19085374261776167 1104 4 0.06313912742807304 1104 5 0.07182948728306826 1104 6 0.04083105050384006 1104 7 0.09066041846046602 1104 8 -0.04207979460562683 1104 9 -0.06615184677954034 1104 10-11 0.06715856357008221 1104 12-13 0.038429389089905897 1104 14-15 0.011923814162635438 1104 16-17 0.02339743915195669 1104 18-19 0.09717546328924698 1104 20-21 0.15243918591158945 1104 22-23 0.0381684907833133 1104 24-25 0.05288853794234427 1104 26-27 0.07782910568007395 1104 28-29 -0.06067653169769471 1104 30-31 -0.017279835099785146 1104 32-33 0.009058380885768713 1104 34-35 0.09106634658053991 1104 36-37 0.14076567667067508 1104 38-39 0.028707031686721507 1104 40-41 -0.045439273458534046 1104 42-43 -0.007409070324385425 1104 44-45 -0.03492665757352853 1104 46-47 -0.04849624671155084 1104 48-49 -0.1964760826255869 1104 50-51 -0.1622299658891464 1104 52-53 -0.06296744980031121 1104 54-55 -0.044152429913097535 1104 56-57 -0.03706348455797581 1104 58-59 -0.018478936821125558 1104 60-61 0.0333835434686236 1104 62-63 0.0963227930302537 1104 64-65 -0.062347851975282254 1104 66-67 -0.0626581692313053 1104 68-69 -0.1562194097045051 1104 70-71 -0.07254785464973423 1104 72-73 0.03157158406590099 1104 74-75 0.0053885583013411065 1104 76-77 -0.16723221142661515 1104 78-79 -6.924787702828894E-4 1104 80-81 -0.01886410545418471 1104 82-83 0.006177351470419978 1104 84-85 -0.09300298350898828 1104 86-87 -0.15097896154149737 1104 88-89 -0.2133082965498332 1104 90-91 -0.2429317406574114 1104 92-93 -0.25538072809763435 1104 94-95 -0.33550047906277314 1104 96-97 -0.26146599670191506 1104 98-99 -0.1752180340217251 1104 100-101 -0.09822383820289815 1104 102-103 -0.1824664260639679 1104 104-105 -0.050664480722211636 1104 106-107 -0.048773391149886436 1104 108-109 -0.08625462165706921 1104 110-111 -0.10245598417784407 1104 112-113 -0.011678847062412956 1104 114-115 -0.09489409869136267 1104 116-117 -0.31999447999176667 1104 118-119 -0.24154059940106976 1104 120-121 -0.37664885331025033 1104 122-123 -0.15233823083944031 1104 124-125 -0.19101217565084028 1105 1 1.888894610715397E-4 1105 2 0.011128265921186653 1105 3 -0.042378070904277365 1105 4 -0.009994948393291736 1105 5 -0.016686830533657826 1105 6 0.049296011239562176 1105 7 0.021368170791937757 1105 8 0.0489745184934165 1105 9 0.09669787404097718 1105 10-11 0.07002251707002927 1105 12-13 0.15998941019010005 1105 14-15 0.10548875200663588 1105 16-17 0.08535189457779069 1105 18-19 -0.062183269902760685 1105 20-21 0.08650356141545501 1105 22-23 0.06593465681796573 1105 24-25 -0.023209020605726494 1105 26-27 -5.302545460565966E-4 1105 28-29 0.04624522837715972 1105 30-31 0.003623790749941236 1105 32-33 0.06779306582599531 1105 34-35 -0.02477820867311209 1105 36-37 -0.08003991289069745 1105 38-39 -0.16203077847123382 1105 40-41 -0.21272550956176417 1105 42-43 -0.09943379338431413 1105 44-45 -0.16426737597547714 1105 46-47 -0.2560569844221803 1105 48-49 -0.22901218685258584 1105 50-51 -0.21905617790630316 1105 52-53 -0.34047732807606224 1105 54-55 -0.22515379346350528 1105 56-57 -0.1506188675962079 1105 58-59 -0.20641002278451026 1105 60-61 -0.27111866110374905 1105 62-63 -0.23896380137819762 1105 64-65 -0.22205493746093197 1105 66-67 -0.21239989088787325 1105 68-69 -0.2109604844210935 1105 70-71 -0.18188817493477671 1105 72-73 -0.14011384386001424 1105 74-75 -0.23640276872183819 1105 76-77 -0.1379631540679256 1105 78-79 -0.05447309644866749 1105 80-81 -0.22094327100114697 1105 82-83 -0.13776928095819585 1105 84-85 -0.1377140003991002 1105 86-87 -0.17768265308052378 1105 88-89 -0.1513911645840338 1105 90-91 -0.11679337512637744 1105 92-93 -0.44561342742979093 1105 94-95 -0.47290804129342234 1105 96-97 -0.3197801706590937 1105 98-99 -0.36216378064022337 1105 100-101 -0.33520541534302595 1105 102-103 -0.2742622319023482 1105 104-105 -0.13011368598133544 1105 106-107 -0.1712515693669232 1105 108-109 -0.2690251172130971 1105 110-111 -0.18771769627960566 1105 112-113 -0.21584066167926608 1105 114-115 -0.04545544353027253 1105 116-117 -0.12174470144429961 1105 118-119 -0.033325457024510285 1105 120-121 -0.2794063105375635 1105 122-123 -0.31685506620814863 1105 124-125 -0.2445041585716261 1106 1 0.025254568430881363 1106 2 0.022247033597089683 1106 3 -0.05226939553784149 1106 4 -0.030351840019406495 1106 5 -0.10191157463690814 1106 6 0.0027034218372037344 1106 7 0.021138473848345996 1106 8 -0.07775359210566535 1106 9 -0.011453496244847372 1106 10-11 0.05817876841591385 1106 12-13 0.07414735555094154 1106 14-15 -0.06255609632160031 1106 16-17 -0.024138178207429917 1106 18-19 0.03926382084188873 1106 20-21 -0.023133660974252734 1106 22-23 0.05911193979024887 1106 24-25 0.05693367862048149 1106 26-27 -0.009312573623823539 1106 28-29 -0.03584989486961376 1106 30-31 -0.17866585709592187 1106 32-33 0.07282486449663139 1106 34-35 -0.1593949736787863 1106 36-37 -0.030310524602370492 1106 38-39 -0.3435416101177253 1106 40-41 -0.2932737674540107 1106 42-43 -0.21257389416384598 1106 44-45 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-0.32513622752851745 2216 56-57 -0.4694441725802747 2216 58-59 -0.42889155166614046 2216 60-61 -0.45041039543511374 2216 62-63 -0.45022643361563297 2216 64-65 -0.43618010012507114 2216 66-67 -0.5772300211267307 2216 68-69 -0.5411400154464872 2216 70-71 -0.6461414972266724 2216 72-73 -0.6670372241194897 2216 74-75 -0.6465028228535985 2216 76-77 -0.5094611698342462 2216 78-79 -0.7050292913086622 2216 80-81 -0.6929551799880898 2216 82-83 -0.5999819076390551 2216 84-85 -0.5968642393316657 2216 86-87 -0.6204795442685196 2216 88-89 -0.6122262993219252 2216 90-91 -0.6783933450133475 2216 92-93 -0.6945307922527562 2216 94-95 -0.6402979584467374 2216 96-97 -0.49617262949566054 2216 98-99 -0.7447417787689119 2216 100-101 -0.7260322331484304 2216 102-103 -0.831106852245135 2216 104-105 -0.8029338072221321 2216 106-107 -0.8120667362970728 2216 108-109 -1.0111894554971528 2216 110-111 -0.9944538738604507 2216 112-113 -1.0037651568378827 2216 114-115 -1.087326000513805 2216 116-117 -1.1894094000092679 2216 118-119 -1.2168849965819177 2216 120-121 -1.305811674959017 2216 122-123 -1.571542302943179 2216 124-125 -1.4377088303933583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 54.0 3 5.0 4 6.0 5 16.0 6 52.0 7 198.0 8 321.0 9 504.0 10 1444.0 11 1861.0 12 1493.0 13 2022.0 14 3478.0 15 3667.0 16 1362.0 17 811.0 18 714.0 19 657.0 20 594.0 21 628.0 22 711.0 23 756.0 24 919.0 25 1172.0 26 1555.0 27 1955.0 28 2455.0 29 3288.0 30 4126.0 31 5389.0 32 6958.0 33 9483.0 34 13333.0 35 21867.0 36 47450.0 37 155029.0 38 152469.0 39 1828.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.02779534916756 21.17977640314021 16.205112497586246 23.587315750105983 2 13.590528815214256 20.808201850742293 45.277722299891266 20.323547034152188 3 18.148591971240265 27.76202205800768 30.40787342165413 23.68151254909793 4 11.22428599960056 19.373321793932938 39.647826376406364 29.754565830060137 5 10.826611559229384 37.631935648734526 34.23060684843232 17.31084594360376 6 25.93990635332756 36.70749839114129 22.823380600492644 14.529214655038503 7 25.409093935157443 32.75214699420811 24.237400971972573 17.601358098661873 8 23.86947162860884 38.24246055522269 21.874930652641858 16.01313716352662 9 24.597341499678226 19.649157845682712 24.35457026829106 31.398930386348 10-11 22.550318443068594 26.161928855158333 32.1871601979451 19.100592503827972 12-13 26.22839136320263 24.50813305816302 30.751170583405457 18.512304995228902 14-15 20.282493398131503 30.580298692940993 27.438807891174577 21.698400017752924 16-17 18.59563278077358 30.782016288307478 32.06366642256396 18.55868450835497 18-19 20.093646672436368 27.17706322259947 34.77764463085014 17.951645474114017 20-21 20.499670451847134 28.04687794734775 31.88095433594383 19.57249726486129 22-23 19.067057528604526 30.542265460646036 32.37959026070284 18.0110867500466 24-25 19.847324856312273 28.488893327119808 34.27778887335508 17.385992943212834 26-27 17.717750704569156 30.598495439717727 33.00412755475667 18.67962630095644 28-29 17.715309677562523 30.749728158356078 30.993054168608392 20.541907995473004 30-31 18.75376556572528 28.252366966654424 33.33181693608607 19.66205053153422 32-33 17.84424028582207 29.589130772474093 34.41970130705901 18.14692763464483 34-35 18.279297871868273 30.110179082617666 33.429531988549364 18.180991056964693 36-37 17.80096753434081 29.74968377604687 32.49794731819897 19.951401371413354 38-39 19.498368950136474 30.414530768035863 31.152719525996936 18.934380755830727 40-41 21.57812395978963 27.04480394114906 33.485897521248035 17.891174577813285 42-43 18.48623038856712 30.385793222821384 33.19519339591238 17.93278299269911 44-45 18.09267026163371 30.87810398775048 31.41069169828906 19.61853405232674 46-47 20.595943456938066 28.596409471184785 31.186561036770744 19.621086035106405 48-49 19.451323702372232 29.502363357965518 30.98606395490757 20.06024898475468 50-51 17.912699997780884 31.32647626656015 31.0526374187249 19.70818631693407 52-53 17.856889243947364 29.743692164303308 32.174844107138895 20.224574484610432 54-55 18.828417992588154 28.828417992588157 31.63781816567916 20.70534584914453 56-57 19.675121496571464 29.834010163548808 28.82331402702883 21.667554312850896 58-59 19.19235292812285 30.980294254710074 28.640569868850275 21.1867829483168 60-61 18.764729378869582 27.777888733550803 33.46059960499745 19.996782282582164 62-63 19.72394203670417 27.68213390142689 32.329738366287195 20.26418569558174 64-65 20.755830725872666 29.36222621663005 29.513126067949315 20.368816989547966 66-67 19.986019572598362 31.594989237290015 28.823203071255797 19.595788118855825 68-69 21.15549342032266 27.869760113618714 32.06655127266272 18.908195193395912 70-71 21.969797838581542 27.78066262787653 30.89785411534962 19.351685418192307 72-73 20.18185651199432 30.63843951800812 29.52632980493975 19.65337416505781 74-75 19.869516010918048 30.48787253400794 31.505891751547832 18.136719703526175 76-77 20.069347358143045 28.864367663049507 32.30665956549719 18.759625413310253 78-79 19.3701040765151 28.341100237445353 31.669773428311476 20.61902225772807 80-81 19.703082351374743 29.703193307147767 31.18412000976411 19.40960433171338 82-83 19.412045358720015 29.09138317466658 32.483079244613094 19.013492222000313 84-85 19.252712868650555 29.52333399906797 31.992099948960345 19.231853183321128 86-87 17.518917959301422 32.06810465348512 29.384861194327943 21.028116192885516 88-89 18.95956771630828 29.377427157535006 31.38561569358454 20.277389432572175 90-91 19.556550713284725 29.209334115947467 32.517950009597755 18.71616516117006 92-93 18.34343030867896 30.961986552160308 31.948050507067883 18.746532632092848 94-95 18.455417069346787 31.581476011730338 30.65232515176094 19.31078176716194 96-97 20.760268956793823 30.470563433415442 29.887047023056613 18.88212058673413 98-99 20.336799637835533 28.44455688112484 32.78254154239455 18.436101938645077 100-101 19.259301997874083 29.16545539678982 32.44120995319875 19.134032652137346 102-103 18.515140520406497 30.870772709671044 31.00968979991279 19.604396970009663 104-105 19.032370097410993 30.022979629019353 31.09772944399506 19.846920829574593 106-107 19.10491385250786 28.932064044421413 31.214165317520116 20.74885678555061 108-109 18.410146762340872 30.077569783401554 30.80256043530507 20.709723018952502 110-111 18.142224816274176 30.15311687547922 31.155994127875168 20.548664180371436 112-113 19.385995476970947 28.59606332240675 30.781676183966105 21.2362650166562 114-115 19.441282015432655 29.49691857355588 28.565943857453235 22.495855553558233 116-117 19.403155652212504 29.71461541875638 27.664994895921176 23.21723403310994 118-119 18.880671951889312 28.526776550476836 30.296309035733504 22.296242461900352 120-121 18.8483627293466 27.290248275739636 31.58481053108107 22.276578463832696 122-123 19.937175865412996 29.192029701102694 29.74737197169252 21.123422461791787 124-125 21.07690788429809 29.668226357480247 28.356599245267944 20.898266512953718 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 37.0 1 33.5 2 141.5 3 181.5 4 71.0 5 31.0 6 60.5 7 130.0 8 214.5 9 244.5 10 207.0 11 167.5 12 183.5 13 242.5 14 277.5 15 335.0 16 426.5 17 497.5 18 585.0 19 711.0 20 855.5 21 1040.5 22 1235.0 23 1513.5 24 1905.5 25 2317.0 26 2874.0 27 3526.0 28 4190.5 29 5100.5 30 6406.5 31 8491.5 32 11860.0 33 16987.5 34 23843.5 35 31149.5 36 36249.5 37 36876.0 38 32868.5 39 26290.5 40 20730.5 41 17124.5 42 14197.0 43 12298.0 44 11445.5 45 10938.5 46 10585.5 47 10217.0 48 9975.0 49 9627.5 50 9183.5 51 8573.5 52 7713.0 53 6835.0 54 6080.5 55 5389.5 56 4516.5 57 3656.5 58 2944.0 59 2303.0 60 1704.0 61 1220.0 62 864.5 63 580.0 64 400.0 65 307.5 66 212.5 67 161.0 68 121.5 69 89.0 70 71.0 71 52.0 72 39.0 73 38.5 74 33.0 75 23.0 76 14.0 77 8.5 78 5.5 79 1.5 80 0.5 81 0.5 82 1.5 83 1.0 84 0.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0193063045070235 2 0.0 3 0.0 4 0.0 5 0.13314692763464483 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002884850098750638 22-23 4.4382309211548276E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.1095577302887069E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 7.766904112020949E-4 92-93 0.0 94-95 7.766904112020949E-4 96-97 0.0 98-99 0.0013314692763464484 100-101 2.2191154605774138E-4 102-103 3.328673190866121E-4 104-105 0.0029958058717795086 106-107 0.00809977143110756 108-109 7.766904112020949E-4 110-111 0.005880655970530147 112-113 0.010429842664713845 114-115 0.005991611743559017 116-117 0.0022191154605774137 118-119 5.547788651443534E-4 120-121 4.4382309211548276E-4 122-123 0.0015533808224041897 124-125 0.001442425049375319 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 450630.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.66269654059346 #Duplication Level Percentage of deduplicated Percentage of total 1 91.35943016384293 70.95219700936075 2 5.881965272094944 9.136185679780377 3 1.158753085375103 2.69975667704889 4 0.49212326955415464 1.5287848057579596 5 0.25947024568566657 1.0075579475999576 6 0.1638534194768507 0.7635179036381533 7 0.1120953568877416 0.6093939379907539 8 0.08283025827362273 0.5146256970146674 9 0.055383784995234164 0.387112867861891 >10 0.3723174774355938 5.397604962527948 >50 0.03432092618859644 1.843162587872816 >100 0.024596663086545543 3.4813071370898494 >500 0.0025740693927780163 1.4371248119039939 >1k 2.860077103086685E-4 0.2416679745520163 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1088 0.24143976211082266 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 991 0.2199143421432217 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 897 0.199054656813794 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 837 0.18573996405032955 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 798 0.1770854137540776 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 646 0.14335485875330095 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 591 0.13114972372012515 No Hit ATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTT 573 0.1271553158910858 No Hit GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA 569 0.12626766970685485 No Hit CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 568 0.1260457581607971 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 479 0.10629563056165812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 1.1095577302887069E-4 16-17 0.0 0.0 0.0 0.0 2.2191154605774138E-4 18-19 0.0 0.0 0.0 0.0 2.2191154605774138E-4 20-21 0.0 0.0 0.0 0.0 2.2191154605774138E-4 22-23 0.0 0.0 0.0 2.2191154605774138E-4 2.2191154605774138E-4 24-25 0.0 0.0 0.0 2.2191154605774138E-4 2.2191154605774138E-4 26-27 0.0 0.0 0.0 2.2191154605774138E-4 2.2191154605774138E-4 28-29 0.0 0.0 0.0 4.4382309211548276E-4 2.2191154605774138E-4 30-31 0.0 0.0 0.0 0.0018862481414908018 2.2191154605774138E-4 32-33 0.0 0.0 0.0 0.004216319375097087 2.2191154605774138E-4 34-35 0.0 0.0 0.0 0.006990213700818854 2.2191154605774138E-4 36-37 0.0 0.0 0.0 0.013647560082551094 2.2191154605774138E-4 38-39 0.0 0.0 0.0 0.026962252846015576 2.2191154605774138E-4 40-41 0.0 0.0 0.0 0.041941282204913125 2.2191154605774138E-4 42-43 0.0 0.0 0.0 0.06269001176131195 2.2191154605774138E-4 44-45 0.0 0.0 0.0 0.08343874131771076 2.2191154605774138E-4 46-47 0.0 0.0 0.0 0.10396555932805185 2.2191154605774138E-4 48-49 0.0 0.0 0.0 0.1310387679470963 2.2191154605774138E-4 50-51 0.0 0.0 0.0 0.16643365954330602 2.2191154605774138E-4 52-53 0.0 0.0 0.0 0.19861083372167854 2.2191154605774138E-4 54-55 0.0 0.0 0.0 0.2366686638705812 2.2191154605774138E-4 56-57 0.0 0.0 0.0 0.28071810576304285 2.2191154605774138E-4 58-59 0.0 0.0 0.0 0.33053724785300576 2.2191154605774138E-4 60-61 0.0 0.0 0.0 0.3953354193018663 2.2191154605774138E-4 62-63 0.0 0.0 0.0 0.4606883696158711 2.2191154605774138E-4 64-65 0.0 0.0 0.0 0.5247098506535295 2.2191154605774138E-4 66-67 0.0 0.0 0.0 0.5976077935334976 2.2191154605774138E-4 68-69 0.0 0.0 0.0 0.6920311563810665 2.2191154605774138E-4 70-71 0.0 0.0 0.0 0.7900051039655593 2.2191154605774138E-4 72-73 0.0 0.0 0.0 0.8819874398064931 2.2191154605774138E-4 74-75 0.0 0.0 0.0 0.9878392472760358 2.2191154605774138E-4 76-77 0.0 0.0 0.0 1.098129285666733 2.2191154605774138E-4 78-79 0.0 0.0 0.0 1.22617224774205 2.2191154605774138E-4 80-81 0.0 0.0 0.0 1.3610944677451569 2.2191154605774138E-4 82-83 0.0 0.0 0.0 1.5038945476333132 2.2191154605774138E-4 84-85 0.0 0.0 0.0 1.654461531633491 2.2191154605774138E-4 86-87 0.0 0.0 0.0 1.8122406408805452 2.2191154605774138E-4 88-89 0.0 0.0 0.0 1.983889221756208 2.2191154605774138E-4 90-91 0.0 0.0 0.0 2.157202139227304 2.2191154605774138E-4 92-93 0.0 0.0 0.0 2.356478707587156 2.2191154605774138E-4 94-95 0.0 0.0 0.0 2.54865410647316 2.2191154605774138E-4 96-97 0.0 0.0 0.0 2.7439362670039724 2.2191154605774138E-4 98-99 0.0 0.0 0.0 2.9699531766637817 2.2191154605774138E-4 100-101 0.0 0.0 0.0 3.214388744646384 2.2191154605774138E-4 102-103 0.0 0.0 0.0 3.453720347069658 2.2191154605774138E-4 104-105 0.0 0.0 0.0 3.7004859862858663 2.2191154605774138E-4 106-107 0.0 0.0 0.0 3.9616758759958284 2.2191154605774138E-4 108-109 0.0 0.0 0.0 4.240174866298293 2.2191154605774138E-4 110-111 0.0 0.0 0.0 4.545858020992832 2.2191154605774138E-4 112-113 0.0 0.0 0.0 4.8758404899806935 2.2191154605774138E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGTAC 15 0.0042342218 59.507214 118-119 GGTATCA 850 0.0 55.349705 1 GTATCAA 1505 0.0 54.60718 1 TACCTGG 535 0.0 54.54431 2 GTACCTG 575 0.0 51.785637 1 ACCTGGG 655 0.0 41.82382 3 ATCAACG 1955 0.0 41.42851 3 TATCAAC 1965 0.0 41.217674 2 TCAACGC 1995 0.0 40.896374 4 CAACGCA 2030 0.0 40.191265 5 TTCAACG 75 8.137515E-5 39.70232 2 GTATAAC 200 0.0 38.709763 1 AACGCAG 2110 0.0 38.34685 6 CCTGGGG 355 0.0 35.22882 4 ACGCAGA 2390 0.0 33.605404 7 CGCAGAG 2400 0.0 33.46538 8 CGGTAGG 90 2.3781149E-4 33.085266 1 TACATGG 2010 0.0 32.887745 2 GTATAGC 220 3.6379788E-12 32.48372 1 GTACATG 2075 0.0 32.14453 1 >>END_MODULE