FastQCFastQC Report
Fri 27 May 2016
SRR939982_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939982_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797850
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA17080.21407532744250174No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC15570.19514946418499718No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA13540.16970608510371624No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA11800.14789747446261828No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA9860.12358212696622173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG256.4392225E-103026.1077108-109
CCGGTCG100.00128311082521.7563108-109
CTGTTCG550.02292.5059108-109
CGCGTCT402.07001E-92257.9163110-111
CCGCTCG352.4756446E-92161.5056108-109
CCGCTCC1850.02044.6674108-109
CGTGTCG150.00202403922007.0367110-111
CTCCGCC850.01770.9148110-111
CCGCGCG750.01681.171108-109
CGTCGCA150.00288662061681.1709108-109
CCGTTCG150.00288662061681.1709108-109
CCGCGCC1900.01592.6882108-109
CGTATCT200.0035978971505.2775110-111
CGGCTCG200.0035978971505.2775110-111
AAGCGCG357.212633E-61441.0037108-109
CGAGGCG357.212633E-61441.0037108-109
CCGCGCT357.212633E-61441.0037108-109
CGTCGAG200.0051310971260.8782108-109
CGTCGCT250.00562109751204.222110-111
CGCCGCC1005.2750693E-111204.222110-111
Produced by FastQC (version 0.11.5)