##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939982_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 797850 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.837974556620917 34.0 31.0 34.0 30.0 34.0 2 32.02829479225419 34.0 31.0 34.0 30.0 34.0 3 32.13819890956947 34.0 31.0 34.0 30.0 34.0 4 35.37857868020305 37.0 35.0 37.0 33.0 37.0 5 35.404906937394244 37.0 35.0 37.0 33.0 37.0 6 35.48904555994235 37.0 37.0 37.0 35.0 37.0 7 35.437087171774145 37.0 37.0 37.0 35.0 37.0 8 35.43397380459986 37.0 37.0 37.0 35.0 37.0 9 37.08966723068246 39.0 39.0 39.0 35.0 39.0 10-11 37.06590837876794 39.0 39.0 39.0 35.0 39.0 12-13 37.00500156671053 39.0 38.5 39.0 34.0 39.0 14-15 38.53034718305446 41.0 39.5 41.0 35.0 41.0 16-17 38.529460424891894 41.0 39.0 41.0 34.5 41.0 18-19 38.54307827285831 41.0 39.5 41.0 34.0 41.0 20-21 38.594524033339596 41.0 39.5 41.0 34.0 41.0 22-23 38.58584633703077 41.0 39.0 41.0 34.0 41.0 24-25 38.60511875665852 41.0 39.0 41.0 34.0 41.0 26-27 38.56357962022936 41.0 39.0 41.0 34.0 41.0 28-29 38.5122121952748 41.0 39.0 41.0 34.0 41.0 30-31 38.41707714482672 41.0 39.0 41.0 34.0 41.0 32-33 38.32555492887134 40.5 39.0 41.0 33.5 41.0 34-35 38.28013285705333 40.0 38.5 41.0 33.0 41.0 36-37 38.44137557184935 41.0 39.0 41.0 34.0 41.0 38-39 38.38972488563013 41.0 39.0 41.0 34.0 41.0 40-41 38.2891614965219 41.0 39.0 41.0 33.0 41.0 42-43 38.24734348561759 41.0 39.0 41.0 33.0 41.0 44-45 38.18161057842953 41.0 39.0 41.0 33.0 41.0 46-47 38.069438490944414 41.0 38.0 41.0 33.0 41.0 48-49 37.98573792066178 40.0 38.0 41.0 33.0 41.0 50-51 37.880453719370806 40.0 38.0 41.0 33.0 41.0 52-53 37.783642915334966 40.0 38.0 41.0 32.5 41.0 54-55 37.65392492323119 40.0 38.0 41.0 32.0 41.0 56-57 37.46126276869086 40.0 38.0 41.0 31.5 41.0 58-59 37.30520586576424 40.0 37.0 41.0 31.0 41.0 60-61 37.102804411856866 40.0 37.0 41.0 31.0 41.0 62-63 36.844323494391176 40.0 36.5 41.0 31.0 41.0 64-65 36.5516231121138 39.5 36.0 41.0 30.0 41.0 66-67 36.24606567650561 39.0 35.5 41.0 30.0 41.0 68-69 35.83413736917967 39.0 35.0 41.0 29.5 41.0 70-71 35.41267280817196 38.0 35.0 40.0 29.0 41.0 72-73 35.016382778717805 37.0 35.0 40.0 29.0 41.0 74-75 34.58734285893338 37.0 34.0 39.0 28.0 41.0 76-77 34.09404085981074 36.0 34.0 39.0 28.0 40.5 78-79 33.60618599987466 36.0 34.0 38.5 26.5 40.0 80-81 33.146619038666415 35.5 34.0 37.0 26.0 39.0 82-83 32.90980322115686 35.0 34.0 37.0 26.0 39.0 84-85 32.42541141818637 35.0 34.0 37.0 26.0 39.0 86-87 31.706521275929056 35.0 34.0 36.0 12.0 37.0 88-89 16.048427649307513 18.0 16.5 18.5 2.0 19.5 90-91 2.742833239330701 2.0 2.0 2.0 2.0 2.0 92-93 2.295165757974557 2.0 2.0 2.0 2.0 2.0 94-95 2.1026207933822145 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05891015705062941 1101 2 0.22145744408950208 1101 3 0.15817594830979687 1101 4 0.20756675937039404 1101 5 0.11311120107487938 1101 6 0.15798011530567635 1101 7 0.31935887603639657 1101 8 0.21159549463533267 1101 9 0.271692657621351 1101 10-11 0.16595605991011553 1101 12-13 0.3314918210093012 1101 14-15 0.2029342076173748 1101 16-17 0.2371942749664271 1101 18-19 0.33914808558445486 1101 20-21 0.1712863258206596 1101 22-23 0.22738479584099025 1101 24-25 0.32138740304797153 1101 26-27 0.34148216223275085 1101 28-29 0.19420409792759585 1101 30-31 0.1353423941422136 1101 32-33 0.21303979390671657 1101 34-35 0.3681192312435897 1101 36-37 0.19644280349609744 1101 38-39 0.14081222653928904 1101 40-41 0.17231578272314607 1101 42-43 0.1310183653390311 1101 44-45 0.2145133318992123 1101 46-47 0.1182033600201251 1101 48-49 0.24415508141915154 1101 50-51 0.252353556959541 1101 52-53 0.1020388277866573 1101 54-55 0.11160404948342517 1101 56-57 0.10998909593753581 1101 58-59 0.0015413691301660037 1101 60-61 0.042685503660216284 1101 62-63 0.01051812581862066 1101 64-65 0.142545071865797 1101 66-67 0.199148583374523 1101 68-69 0.13089033188395405 1101 70-71 0.008193297548487521 1101 72-73 0.11972148197921229 1101 74-75 0.16146733978272465 1101 76-77 0.21834172232957627 1101 78-79 0.034735743550101006 1101 80-81 -0.01848554909106781 1101 82-83 0.1635812395520091 1101 84-85 0.11576411088491767 1101 86-87 0.07611443077961155 1101 88-89 0.7372660124388979 1101 90-91 -0.018937671672316636 1101 92-93 -0.22535731011378912 1101 94-95 -0.05239153018737186 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 0.04167609293978458 1102 2 0.07553308019522831 1102 3 0.11939217992954099 1102 4 0.2052268781692277 1102 5 0.052736566780261285 1102 6 -0.03054338482730401 1102 7 0.08231846163361922 1102 8 0.1387583807632211 1102 9 0.21074492657787403 1102 10-11 0.1157103565525901 1102 12-13 0.1821684551266003 1102 14-15 0.13014933737153456 1102 16-17 0.14467081282880656 1102 18-19 0.12614290554975582 1102 20-21 0.14138872332816987 1102 22-23 0.10626190616686415 1102 24-25 0.15042251076483382 1102 26-27 0.1705125205290443 1102 28-29 0.2189723262037404 1102 30-31 0.10878680059129664 1102 32-33 0.02016740767116687 1102 34-35 0.05962378435477689 1102 36-37 0.20229725591956793 1102 38-39 0.26427024799399845 1102 40-41 0.16370350008830314 1102 42-43 0.21276855848213927 1102 44-45 0.2899657933655817 1102 46-47 0.32387226841329664 1102 48-49 0.23293061747042287 1102 50-51 0.4386163329008781 1102 52-53 0.4018175047880561 1102 54-55 0.32145878053957233 1102 56-57 0.34815353987536923 1102 58-59 0.34801159976203166 1102 60-61 0.3776098928847347 1102 62-63 0.2788683037635806 1102 64-65 0.5202854875350837 1102 66-67 0.5023056815418769 1102 68-69 0.631517255399288 1102 70-71 0.5702365616036005 1102 72-73 0.4039711368546506 1102 74-75 0.5172036899480048 1102 76-77 0.46275957001881807 1102 78-79 0.5460298656672009 1102 80-81 0.5734569005967387 1102 82-83 0.5166373333752219 1102 84-85 0.4119063148693627 1102 86-87 0.5049475298081987 1102 88-89 1.065324322658384 1102 90-91 0.24904680088869746 1102 92-93 0.11636666622776382 1102 94-95 0.18491643490755338 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 -0.12418137314440258 1103 2 -0.028896774931517655 1103 3 0.04981295399910124 1103 4 6.974015733689498E-4 1103 5 -0.13298149064713982 1103 6 -0.06336551215244413 1103 7 0.08567397059341175 1103 8 -0.06196045951394069 1103 9 -0.0811390704380841 1103 10-11 -0.10596698347742972 1103 12-13 -0.07346537687691068 1103 14-15 -0.1832645629701517 1103 16-17 -0.11273402487569228 1103 18-19 -0.1698802176830725 1103 20-21 -0.15451965869023354 1103 22-23 -0.05730725937669945 1103 24-25 -0.11899753571495353 1103 26-27 -0.18223727761852615 1103 28-29 -0.09213293485362328 1103 30-31 -0.07866350379911324 1103 32-33 -0.05222432113738762 1103 34-35 -0.029746190440526732 1103 36-37 -0.032219587630208935 1103 38-39 -0.019596830257206932 1103 40-41 0.003374718540221977 1103 42-43 -0.05892827604184703 1103 44-45 -0.1759391809538755 1103 46-47 -0.11330173606275906 1103 48-49 -0.12301006093796474 1103 50-51 -0.2100307185185386 1103 52-53 -0.1103454476914294 1103 54-55 -0.2604395840719391 1103 56-57 -0.232372151411667 1103 58-59 -0.07403690315292977 1103 60-61 -0.15642373281484367 1103 62-63 -0.20279996441925618 1103 64-65 -0.101097669836129 1103 66-67 -0.08975322866986346 1103 68-69 -0.12840383027517532 1103 70-71 -0.0433296106908827 1103 72-73 0.08377292236610145 1103 74-75 -0.06036290600634686 1103 76-77 0.004062569714889719 1103 78-79 -0.006832831695362529 1103 80-81 0.04085197570426402 1103 82-83 0.02028641099401085 1103 84-85 0.033507058423133174 1103 86-87 0.14512330260370732 1103 88-89 0.43435931450753884 1103 90-91 -0.43159271061305393 1103 92-93 -0.29838598394891447 1103 94-95 -0.10660299972142218 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.12225885315064033 1104 2 -0.04361010484901584 1104 3 -0.008104724422487664 1104 4 -0.06471060484246038 1104 5 -0.019505286380322673 1104 6 0.03479290824750336 1104 7 -0.110224801934379 1104 8 0.025036354850385578 1104 9 -0.034483245328743806 1104 10-11 0.04579476958753048 1104 12-13 0.05057094976584864 1104 14-15 0.007945236015494572 1104 16-17 -0.021180414859877317 1104 18-19 -0.030647889050229082 1104 20-21 -0.14124469154906194 1104 22-23 -0.139495546039349 1104 24-25 -0.021974879830438 1104 26-27 0.04185437066947628 1104 28-29 0.08421650487548504 1104 30-31 0.08527346665393054 1104 32-33 0.20123940791278017 1104 34-35 0.22011123565495438 1104 36-37 0.1276324919438494 1104 38-39 0.15361895386214286 1104 40-41 0.08158652989712323 1104 42-43 0.03674623961529733 1104 44-45 0.08568096355072186 1104 46-47 0.12013332969203105 1104 48-49 0.08474647926861678 1104 50-51 0.003415040279094228 1104 52-53 0.10053620854209555 1104 54-55 0.09792883580821155 1104 56-57 -0.033359401443220804 1104 58-59 -0.02853169400741251 1104 60-61 0.061930058388639964 1104 62-63 -0.033781570900430324 1104 64-65 -0.035632485344017084 1104 66-67 0.14895558640733242 1104 68-69 0.0867836321320965 1104 70-71 -0.10064320644930547 1104 72-73 -0.021324837292915788 1104 74-75 -0.0841281937160332 1104 76-77 0.039750600190060936 1104 78-79 0.10783334487350515 1104 80-81 0.09520345008180442 1104 82-83 0.09091098865594205 1104 84-85 0.206286581246097 1104 86-87 0.22604550329133133 1104 88-89 0.449100862176973 1104 90-91 -0.6132029046472272 1104 92-93 -0.29838598394891447 1104 94-95 -0.10660299972142218 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.4634820705724856 1105 2 -0.35782828773158215 1105 3 -0.32635785637424775 1105 4 -0.27989764383086424 1105 5 -0.3647297917075676 1105 6 -0.35728354650018446 1105 7 -0.20210660734358044 1105 8 -0.4989933255149097 1105 9 -0.39224287534865 1105 10-11 -0.44971512386756274 1105 12-13 -0.39709179464347244 1105 14-15 -0.6037015266586678 1105 16-17 -0.4329845068865694 1105 18-19 -0.39192730598033165 1105 20-21 -0.42855809161930836 1105 22-23 -0.5826418403842695 1105 24-25 -0.38910475338154527 1105 26-27 -0.4258528650172053 1105 28-29 -0.4721081350964198 1105 30-31 -0.49346584958538386 1105 32-33 -0.6415856418472003 1105 34-35 -0.34363569093444113 1105 36-37 -0.4450053724748386 1105 38-39 -0.46314576923820283 1105 40-41 -0.4434244465693382 1105 42-43 -0.39508697663572434 1105 44-45 -0.5259921426429415 1105 46-47 -0.4595741108887026 1105 48-49 -0.4874757429536061 1105 50-51 -0.5408735383416783 1105 52-53 -0.41421908943237895 1105 54-55 -0.5052148241847618 1105 56-57 -0.3956127968301786 1105 58-59 -0.7476761150353894 1105 60-61 -0.644119438157432 1105 62-63 -0.596172538000296 1105 64-65 -0.6502999715868398 1105 66-67 -0.6645586390931939 1105 68-69 -0.6286931855707465 1105 70-71 -0.5744204280861211 1105 72-73 -0.6707284492821444 1105 74-75 -0.6021208175305759 1105 76-77 -0.6140955536560924 1105 78-79 -0.6734825139252294 1105 80-81 -0.5888996994323037 1105 82-83 -0.6483679306743468 1105 84-85 -0.6804626284483213 1105 86-87 -0.7140807992493556 1105 88-89 0.3951654330703107 1105 90-91 -0.7465362379805605 1105 92-93 -0.29838598394891447 1105 94-95 -0.10660299972142218 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.30726969637043666 1106 2 -0.27982645897581904 1106 3 -0.22290253026507045 1106 4 -0.3175362203546399 1106 5 -0.3868353029640659 1106 6 -0.28360229522233027 1106 7 -0.3583814545686437 1106 8 -0.2965225174547541 1106 9 -0.239675234102819 1106 10-11 -0.39525000653186737 1106 12-13 -0.2175372272975551 1106 14-15 -0.3355360753762682 1106 16-17 -0.37999531471955095 1106 18-19 -0.29761433885249744 1106 20-21 -0.2706170604736684 1106 22-23 -0.3072337779566823 1106 24-25 -0.3946627226675403 1106 26-27 -0.46347156376093324 1106 28-29 -0.4268372009543384 1106 30-31 -0.33726767379765477 1106 32-33 -0.2406728257904973 1106 34-35 -0.3099755101034347 1106 36-37 -0.2756523658186225 1106 38-39 -0.2726981784251379 1106 40-41 -0.1515554524985845 1106 42-43 -0.40035032882455823 1106 44-45 -0.2992150691065092 1106 46-47 -0.5409203761377981 1106 48-49 -0.3423679485920772 1106 50-51 -0.37003182794389744 1106 52-53 -0.43494025529257385 1106 54-55 -0.31584205333581394 1106 56-57 -0.3633632284619779 1106 58-59 -0.39271717684961516 1106 60-61 -0.3278007746257714 1106 62-63 -0.12025943797530658 1106 64-65 -0.26011902699473666 1106 66-67 -0.2695161574375504 1106 68-69 -0.3960649454926042 1106 70-71 -0.3790907125087557 1106 72-73 -0.2501295317692467 1106 74-75 -0.2969997334532408 1106 76-77 -0.40057979909555996 1106 78-79 -0.4092606722205119 1106 80-81 -0.2513267195826927 1106 82-83 -0.33562300802730505 1106 84-85 -0.21700082786220776 1106 86-87 -0.24143759404251242 1106 88-89 0.6497131851773545 1106 90-91 -0.18268880846148905 1106 92-93 -0.27392495575654285 1106 94-95 -0.10660299972142218 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.1971192707390763 1107 2 -0.1872742923915922 1107 3 -0.09075717650797088 1107 4 -0.171157732078413 1107 5 -0.0969726469208112 1107 6 -0.10307518549569039 1107 7 -0.2252849540528672 1107 8 -0.1261003200332098 1107 9 -0.19817566538927878 1107 10-11 -0.23624668024356765 1107 12-13 -0.22309625767292118 1107 14-15 -0.18660666054954334 1107 16-17 -0.21699083560866939 1107 18-19 -0.24190718576556236 1107 20-21 -0.3561763225036998 1107 22-23 -0.2481812591211181 1107 24-25 -0.23028977775092585 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8665.0 3 1479.0 4 936.0 5 738.0 6 819.0 7 851.0 8 1003.0 9 1162.0 10 1462.0 11 1745.0 12 2199.0 13 2716.0 14 3129.0 15 3519.0 16 3936.0 17 4666.0 18 5906.0 19 7343.0 20 7822.0 21 10977.0 22 13301.0 23 16528.0 24 25297.0 25 42869.0 26 75678.0 27 208448.0 28 332200.0 29 11964.0 30 492.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.89039293100207 21.416306323243717 16.307200601616845 23.38610014413737 2 12.784891357752354 20.74881431049523 45.268271650740495 21.19802268101192 3 18.400977995110026 26.69939188765595 29.9247696069212 24.974860510312833 4 11.52666998534182 18.931119099895046 40.03927238256216 29.50293853220097 5 12.05033806151315 38.4790742932809 34.62233914491503 14.848248500290914 6 27.594284639969917 37.529986839631505 21.361784796640972 13.513943723757599 7 26.632073698063547 32.20429905370683 23.474713291972176 17.688913956257444 8 23.552171460800903 39.37381713354641 21.454784733972552 15.619226671680138 9 24.685467193081408 19.661089177163625 23.061979068747256 32.59146456100771 10-11 23.02320130652271 27.21249669421177 31.10944133118922 18.6548606680763 12-13 25.64814750984387 24.909771048147384 30.03021314651026 19.411868295498486 14-15 20.265526101397505 30.526602744876858 26.249044306573914 22.95882684715172 16-17 19.510018531133493 31.04055730018243 30.431229566088213 19.018194602595862 18-19 20.84937221186737 28.7265872065841 31.465428095477442 18.95861248607109 20-21 21.535589326304272 29.19028540523332 28.97288333800224 20.30124193046017 22-23 20.353752700240836 31.12131358765576 29.28925462385888 19.235679088244527 24-25 21.04017045810616 29.32299304380523 30.79582628313593 18.841010214952686 26-27 19.009274926364604 31.05270414238265 30.07119132669048 19.866829604562263 28-29 19.301078907656134 31.008442753219928 28.316375130977985 21.374103208145954 30-31 20.03935576862819 29.36278749138309 29.990098389421572 20.60775835056715 32-33 19.236100927377763 30.397284672008585 30.951091758884292 19.415522641729368 34-35 19.472606971995955 30.83208727465974 30.425619384119052 19.269686369225255 36-37 18.894552728649156 30.422861970122167 29.92915952768005 20.753425773548628 38-39 20.399260512627688 30.8327379833302 28.944225104969608 19.823776399072507 40-41 22.02494203171022 28.22422761170646 30.731841824904432 19.018988531678886 42-43 19.419001065363165 30.90110923105847 30.652566271855612 19.027323431722753 44-45 18.93037538384408 31.138747884940777 29.47690668672056 20.45397004449458 46-47 20.836874099141443 29.283449269912893 29.397505796828977 20.48217083411669 48-49 20.01719623638057 29.804386544321044 29.46428459520536 20.71413262409303 50-51 18.87104021497789 31.279458093287015 29.63484968029685 20.214652011438247 52-53 18.97305257880554 30.04756533182929 30.465313028764808 20.514069060600363 54-55 19.524941451183416 29.3333801264291 30.33270517780228 20.808973244585214 56-57 20.104280253180423 30.03866641599298 28.15078022184621 21.706273108980383 58-59 19.974681957761483 30.714420003760107 27.834618035971676 21.476280002506737 60-61 19.47226922353826 28.48423889202231 31.36228614401203 20.6812057404274 62-63 20.249482985523596 28.450836623425456 30.616343924296547 20.6833364667544 64-65 20.918092373253117 29.75578116187253 28.603622234755903 20.722504230118442 66-67 20.585260387290845 31.420693112740487 27.831484614902553 20.162561885066115 68-69 21.33257211900011 28.65621020545267 30.439381937411635 19.571835738135583 70-71 22.16826471141192 28.46286896033089 29.52979883436736 19.83906749388983 72-73 20.920473773265652 30.355517954502726 28.575860124083473 20.14814814814815 74-75 20.175847590399197 30.700006266842138 30.12095005326816 19.00319608949051 76-77 20.5704079714232 29.23569593281945 30.66315723506925 19.5307388606881 78-79 20.09879062328837 29.053492578169553 30.01658208508126 20.831134713460816 80-81 20.61107977690042 29.87873660462493 29.626057529610826 19.88412608886382 82-83 20.156747336103713 29.653585379105827 30.736503165717128 19.453164119073328 84-85 20.0942032300578 29.500551169111468 30.681287636327404 19.72395796450333 86-87 18.862611871596695 31.623854493131816 28.51604727983049 20.997486355440998 88-89 28.862113133128055 18.865348256795894 35.68861197678469 16.583926633291362 90-91 30.36414213270493 15.506762129354264 17.427184754997715 36.70191098294309 92-93 22.07343476116721 17.896134785667194 36.88120451598861 23.149225937176993 94-95 15.280410921625545 7.109148222060958 27.897428338171267 49.713012518142236 96-97 26.19171657697249 7.144016192534012 15.094608954939808 51.569658275553685 98-99 56.703852392906015 14.086472672569258 18.99643850761688 10.213236426907846 100-101 30.192780919866095 7.081239754886683 13.021547176749968 49.704432148497254 102-103 22.57780880211025 2.0442051991113317 37.63898863659245 37.73899736218596 104-105 40.06953411642469 4.588652251991238 42.58040506619978 12.761408565384299 106-107 5.47318622739388 1.7403218879731692 6.379135224305882 86.40735666032707 108-109 17.831035380279022 11.188637698284943 22.563343985969208 48.41698293546683 110-111 40.016247938196216 2.772933523132257 25.661369140420394 31.549449398251138 112-113 26.74783177875961 2.675568646702667 39.228015054819174 31.348584519718543 114-115 28.048090052286273 3.9835576552531298 23.902773218389164 44.06557907407143 116-117 19.41630878858971 4.140000783878969 20.27092350274451 56.17276692478681 118-119 19.739079837435998 4.2422554070053495 15.49817239062988 60.52049236492877 120-121 25.027945844316825 4.202286274346516 29.951859479646775 40.817908401689884 122-123 33.95125836502346 1.6187345290989592 33.945011439682034 30.484995666195548 124-125 38.472605082597234 2.0058250669027546 19.888855912593815 39.632713937906196 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 8.0 2 18.5 3 31.0 4 42.0 5 44.0 6 42.0 7 39.5 8 34.5 9 32.0 10 34.0 11 41.5 12 50.5 13 62.5 14 82.0 15 100.5 16 136.5 17 214.5 18 336.5 19 474.0 20 665.5 21 1015.0 22 1492.0 23 2179.5 24 3003.0 25 4062.0 26 5467.0 27 7371.0 28 9844.5 29 12926.5 30 16965.5 31 22098.0 32 28376.0 33 35196.0 34 41434.5 35 45493.0 36 46914.5 37 46154.0 38 43495.5 39 40481.0 40 37719.0 41 34671.0 42 31453.5 43 28526.0 44 26240.0 45 24423.0 46 22895.5 47 21465.5 48 20026.0 49 18584.5 50 17088.5 51 15598.5 52 14097.0 53 12386.5 54 10593.5 55 9116.0 56 7634.0 57 6271.5 58 5167.0 59 4168.0 60 3252.0 61 2462.5 62 1939.0 63 1455.0 64 1047.0 65 770.5 66 596.5 67 452.5 68 333.0 69 253.0 70 189.0 71 131.5 72 90.5 73 66.0 74 59.5 75 52.5 76 34.0 77 22.5 78 15.5 79 9.0 80 3.5 81 4.0 82 3.5 83 1.5 84 2.0 85 1.5 86 1.0 87 0.5 88 0.5 89 2.5 90 2.5 91 1.5 92 1.5 93 1.0 94 1.0 95 1.0 96 1.0 97 2.5 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 2.5067368552986153E-4 3 0.037601052829479224 4 0.04299053706837125 5 0.04537193708090493 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.773578993545153E-4 12-13 0.002318731591151219 14-15 0.0 16-17 4.3867894967725764E-4 18-19 0.006141505295481606 20-21 0.0024440684339161497 22-23 5.640157924421883E-4 24-25 0.0 26-27 0.0 28-29 7.520210565895845E-4 30-31 0.0 32-33 0.0011280315848843767 34-35 1.2533684276493077E-4 36-37 1.2533684276493077E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.2533684276493077E-4 50-51 6.266842138246538E-5 52-53 0.0 54-55 0.0014413736917967036 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.5067368552986153E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.2533684276493077E-4 80-81 0.0 82-83 6.893526352071192E-4 84-85 6.266842138246538E-5 86-87 5.640157924421883E-4 88-89 19.221846211693926 90-91 26.309017985836935 92-93 40.401015228426395 94-95 44.731465814376136 96-97 48.634643103340224 98-99 93.08472770570908 100-101 51.944037099705454 102-103 53.316099517453154 104-105 74.9088801153099 106-107 81.61346117691295 108-109 12.599674124208812 110-111 38.982139499906 112-113 52.12915961646926 114-115 86.82778717804098 116-117 66.4224478285392 118-119 72.10522027950115 120-121 80.4342294917591 122-123 75.92348185749202 124-125 50.16707401140565 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 797850.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 79.81084665685991 #Duplication Level Percentage of deduplicated Percentage of total 1 91.07781333629585 72.68997394025217 2 6.386406147084736 10.194089633868147 3 1.1693245160254901 2.7997433892185195 4 0.43213417331075343 1.3795597696517385 5 0.239217290052475 0.954606722702383 6 0.1550213956108487 0.7423433300177917 7 0.09798235127362019 0.5474038088804238 8 0.0696672194840315 0.44481598169998726 9 0.04819371046048163 0.34617427518479443 >10 0.27635499622821996 4.111259683507702 >50 0.02794705297072591 1.562306780670337 >100 0.01836790483954007 2.979778946335251 >500 9.419438179666121E-4 0.5213548086207909 >1k 6.279625453110748E-4 0.7265889293900424 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1708 0.21407532744250174 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1557 0.19514946418499718 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1354 0.16970608510371624 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1180 0.14789747446261828 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 986 0.12358212696622173 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 6.266842138246537E-5 0.0 0.0 0.0 0.0 12-13 1.2533684276493074E-4 0.0 0.0 0.0 0.0 14-15 1.2533684276493074E-4 0.0 0.0 0.0 0.0 16-17 1.2533684276493074E-4 0.0 0.0 0.0 0.0 18-19 1.2533684276493074E-4 0.0 0.0 3.1334210691232685E-4 0.0 20-21 1.2533684276493074E-4 0.0 0.0 3.7601052829479227E-4 0.0 22-23 1.2533684276493074E-4 0.0 0.0 3.7601052829479227E-4 0.0 24-25 1.2533684276493074E-4 0.0 0.0 3.7601052829479227E-4 0.0 26-27 1.2533684276493074E-4 0.0 0.0 5.640157924421884E-4 0.0 28-29 1.2533684276493074E-4 0.0 0.0 6.893526352071192E-4 0.0 30-31 1.2533684276493074E-4 0.0 0.0 0.0011280315848843769 0.0 32-33 1.2533684276493074E-4 0.0 0.0 0.003446763176035595 0.0 34-35 1.2533684276493074E-4 0.0 0.0 0.006454847402393934 0.0 36-37 1.2533684276493074E-4 0.0 0.0 0.010528294792254183 0.0 38-39 1.2533684276493074E-4 0.0 0.0 0.021933947483862883 0.0 40-41 1.2533684276493074E-4 0.0 0.0 0.03427962649620856 0.0 42-43 1.2533684276493074E-4 0.0 0.0 0.04649996866578931 0.0 44-45 1.2533684276493074E-4 0.0 0.0 0.06385912138873222 0.0 46-47 1.2533684276493074E-4 0.0 0.0 0.08209563201102965 0.0 48-49 1.2533684276493074E-4 0.0 0.0 0.10321489001692047 0.0 50-51 1.2533684276493074E-4 0.0 0.0 0.1281569217271417 0.0 52-53 1.2533684276493074E-4 0.0 0.0 0.15284827975183304 0.0 54-55 1.2533684276493074E-4 0.0 0.0 0.18104906937394247 0.0 56-57 1.2533684276493074E-4 0.0 0.0 0.21771009588268472 0.0 58-59 1.2533684276493074E-4 0.0 0.0 0.2586325750454346 0.0 60-61 1.2533684276493074E-4 0.0 0.0 0.31033402268596855 0.0 62-63 1.2533684276493074E-4 0.0 0.0 0.3578993545152598 0.0 64-65 1.2533684276493074E-4 0.0 0.0 0.40935012847026386 0.0 66-67 1.2533684276493074E-4 0.0 0.0 0.4675064235131917 0.0 68-69 1.2533684276493074E-4 0.0 0.0 0.5517327818512252 0.0 70-71 1.2533684276493074E-4 0.0 0.0 0.6403459296860312 0.0 72-73 2.506736855298615E-4 0.0 0.0 0.7240709406530049 0.0 74-75 3.7601052829479227E-4 0.0 0.0 0.8069812621420066 0.0 76-77 3.7601052829479227E-4 0.0 0.0 0.9051826784483299 0.0 78-79 3.7601052829479227E-4 0.0 0.0 0.9335088049132043 0.0 80-81 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 82-83 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 84-85 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 86-87 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 88-89 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 90-91 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 92-93 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 94-95 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 96-97 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 98-99 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 100-101 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 102-103 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 104-105 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 106-107 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 108-109 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 110-111 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 112-113 3.7601052829479227E-4 0.0 0.0 0.933884815441499 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTGCG 25 6.4392225E-10 3026.1077 108-109 CCGGTCG 10 0.0012831108 2521.7563 108-109 CTGTTCG 55 0.0 2292.5059 108-109 CGCGTCT 40 2.07001E-9 2257.9163 110-111 CCGCTCG 35 2.4756446E-9 2161.5056 108-109 CCGCTCC 185 0.0 2044.6674 108-109 CGTGTCG 15 0.0020240392 2007.0367 110-111 CTCCGCC 85 0.0 1770.9148 110-111 CCGCGCG 75 0.0 1681.171 108-109 CGTCGCA 15 0.0028866206 1681.1709 108-109 CCGTTCG 15 0.0028866206 1681.1709 108-109 CCGCGCC 190 0.0 1592.6882 108-109 CGTATCT 20 0.003597897 1505.2775 110-111 CGGCTCG 20 0.003597897 1505.2775 110-111 AAGCGCG 35 7.212633E-6 1441.0037 108-109 CGAGGCG 35 7.212633E-6 1441.0037 108-109 CCGCGCT 35 7.212633E-6 1441.0037 108-109 CGTCGAG 20 0.005131097 1260.8782 108-109 CGTCGCT 25 0.0056210975 1204.222 110-111 CGCCGCC 100 5.2750693E-11 1204.222 110-111 >>END_MODULE