FastQCFastQC Report
Fri 27 May 2016
SRR939982_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939982_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797850
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17200.21557936955568088No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA16350.20492573792066177No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC14950.1873785799335715No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA12920.16193520085229055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12400.15541768502851414No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA11750.14727079024879364No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA9870.12370746380898665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33300.045.43491
GGTATCA19800.037.905781
ATCAACG39850.037.51853
TCAACGC39850.037.51854
TATCAAC40250.037.1456452
CAACGCA40700.036.588595
AACGCAG41350.035.9705546
ACGCAGA47050.031.6128047
CGCAGAG47300.031.4457198
TAGGACC13950.029.8898644
TACATGG35000.029.7831132
CGAAATG1403.0661467E-629.7831125
GTACATG36150.029.0004311
GCAGAGT51600.028.594649
CCTACCG1055.8657554E-428.364873
ACATGGG35000.027.9110323
GTACCTG7500.027.0033551
ACACCGT900.0095309226.4423546
TATCGTG900.0095309226.4423547
TACCTGG7600.025.8642832
Produced by FastQC (version 0.11.5)