##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939982_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 797850 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.81373566459861 34.0 31.0 34.0 31.0 34.0 2 32.92414363602181 34.0 31.0 34.0 31.0 34.0 3 32.948979131415676 34.0 33.0 34.0 31.0 34.0 4 36.35203108353701 37.0 37.0 37.0 35.0 37.0 5 36.252776837751455 37.0 37.0 37.0 35.0 37.0 6 36.39052704142382 37.0 37.0 37.0 35.0 37.0 7 36.38697875540515 37.0 37.0 37.0 35.0 37.0 8 36.41841448893902 37.0 37.0 37.0 35.0 37.0 9 38.25388356207307 39.0 39.0 39.0 37.0 39.0 10-11 38.243609701071634 39.0 39.0 39.0 37.0 39.0 12-13 38.22768064172463 39.0 39.0 39.0 37.0 39.0 14-15 39.76091307889955 41.0 40.0 41.0 37.0 41.0 16-17 39.78273234317228 41.0 40.0 41.0 37.5 41.0 18-19 39.8300770821583 41.0 40.0 41.0 38.0 41.0 20-21 39.84511624992167 41.0 40.0 41.0 38.0 41.0 22-23 39.79294917591026 41.0 40.0 41.0 37.5 41.0 24-25 39.771950241273416 41.0 40.0 41.0 37.0 41.0 26-27 39.68341542896535 41.0 40.0 41.0 37.0 41.0 28-29 39.6058657642414 41.0 40.0 41.0 37.0 41.0 30-31 39.475456539449766 41.0 39.5 41.0 36.5 41.0 32-33 39.3473616594598 41.0 39.0 41.0 36.0 41.0 34-35 39.27493576486808 41.0 39.0 41.0 35.0 41.0 36-37 39.25832675314909 41.0 39.5 41.0 35.5 41.0 38-39 39.30572288024065 41.0 40.0 41.0 35.0 41.0 40-41 39.215539261766 41.0 40.0 41.0 35.0 41.0 42-43 39.13786614025193 41.0 40.0 41.0 35.0 41.0 44-45 39.00569029266153 41.0 40.0 41.0 35.0 41.0 46-47 38.79358526038729 41.0 39.0 41.0 35.0 41.0 48-49 38.591264022059285 41.0 39.0 41.0 35.0 41.0 50-51 38.46034091621232 41.0 39.0 41.0 35.0 41.0 52-53 38.363272544964595 41.0 39.0 41.0 35.0 41.0 54-55 38.27498088613147 41.0 39.0 41.0 35.0 41.0 56-57 38.13788619414677 41.0 39.0 41.0 34.5 41.0 58-59 37.995500407344736 40.5 38.0 41.0 34.0 41.0 60-61 37.83856614651877 40.0 38.0 41.0 34.0 41.0 62-63 37.64057153600301 40.0 37.5 41.0 34.0 41.0 64-65 37.40463432976124 40.0 37.0 41.0 33.5 41.0 66-67 37.14442564391803 39.5 36.5 41.0 33.0 41.0 68-69 36.81896847778404 39.0 36.0 41.0 33.0 41.0 70-71 36.455322429028016 39.0 35.5 41.0 32.0 41.0 72-73 36.11628814940151 37.5 35.0 40.0 32.0 41.0 74-75 35.76030519521213 37.0 35.0 39.5 32.0 41.0 76-77 35.35797142319985 37.0 35.0 39.0 31.0 41.0 78-79 34.96950178605001 36.0 35.0 39.0 31.0 40.5 80-81 34.67388168202043 36.0 35.0 37.5 31.0 39.0 82-83 34.41219903490631 35.5 35.0 37.0 31.0 39.0 84-85 34.09444507112866 35.0 35.0 37.0 31.0 39.0 86-87 33.83966347057718 35.0 35.0 36.5 31.0 37.0 88-89 33.594583568339914 35.0 35.0 36.0 31.0 37.0 90-91 33.38171962148273 35.0 34.5 36.0 31.0 37.0 92-93 33.2397280190512 35.0 34.0 35.5 31.0 36.0 94-95 33.13033903615968 35.0 34.0 35.0 31.0 36.0 96-97 32.984382402707276 35.0 34.0 35.0 30.0 36.0 98-99 32.84536943034405 35.0 34.0 35.0 30.0 36.0 100-101 32.84021369931691 35.0 34.0 35.0 30.0 36.0 102-103 32.00204737732656 34.5 32.5 35.0 28.0 35.0 104-105 32.67747383593407 35.0 34.0 35.0 29.5 35.0 106-107 32.74873472457229 35.0 34.0 35.0 30.0 35.0 108-109 32.74566271855612 35.0 34.0 35.0 30.0 35.0 110-111 32.72833490004386 35.0 34.0 35.0 30.0 35.0 112-113 32.642098765432095 35.0 34.0 35.0 29.5 35.0 114-115 32.579617722629564 35.0 34.0 35.0 29.0 35.0 116-117 32.52426959954879 35.0 34.0 35.0 29.0 35.0 118-119 32.43986714294667 35.0 34.0 35.0 29.0 35.0 120-121 32.33769129535627 35.0 34.0 35.0 29.0 35.0 122-123 32.163631635019115 35.0 34.0 35.0 29.0 35.0 124-125 30.10241524096008 33.5 30.5 34.5 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.030632335667029054 1101 2 0.10895030750348411 1101 3 0.018270308950448566 1101 4 0.03390367829047847 1101 5 -0.05728005257468283 1101 6 -0.09531795610172367 1101 7 0.05011702905056126 1101 8 0.030540282354820647 1101 9 0.1441180103265225 1101 10-11 0.17257668401516213 1101 12-13 0.07691398446974063 1101 14-15 0.19629397645820035 1101 16-17 0.10802336166332793 1101 18-19 0.17952598963704247 1101 20-21 0.13650660011799687 1101 22-23 0.20596421556152222 1101 24-25 0.14537711074729032 1101 26-27 0.09685517310494873 1101 28-29 0.17694375345274693 1101 30-31 0.26775974143077264 1101 32-33 0.218018798647023 1101 34-35 0.29994735370006254 1101 36-37 0.21041911603727215 1101 38-39 0.24499193497008775 1101 40-41 0.3219005023037198 1101 42-43 0.28658069200614733 1101 44-45 0.35688814746487907 1101 46-47 0.37122775899871385 1101 48-49 0.37185183280813305 1101 50-51 0.46497739128820825 1101 52-53 0.4088558120811072 1101 54-55 0.396581179644798 1101 56-57 0.38812086972027515 1101 58-59 0.3809968615444319 1101 60-61 0.36803454364067534 1101 62-63 0.35711597560590036 1101 64-65 0.325307192855405 1101 66-67 0.29472535184375204 1101 68-69 0.33991956635193077 1101 70-71 0.32599862424576287 1101 72-73 0.2879088696154213 1101 74-75 0.33943741527484406 1101 76-77 0.317756450826856 1101 78-79 0.28383230916600155 1101 80-81 0.24704309359486842 1101 82-83 0.29975247109560144 1101 84-85 0.3673386006722623 1101 86-87 0.29523167508047976 1101 88-89 0.2749307077190082 1101 90-91 0.2918207371489885 1101 92-93 0.31241410726090635 1101 94-95 0.4099163484276289 1101 96-97 0.3334333808867598 1101 98-99 0.36592626777982673 1101 100-101 0.4045971065768441 1101 102-103 0.5786539865755884 1101 104-105 0.3232590088198677 1101 106-107 0.3756480240843203 1101 108-109 0.39240491284544987 1101 110-111 0.4513452659786168 1101 112-113 0.4766550467750932 1101 114-115 0.5035522555911527 1101 116-117 0.5199021077696315 1101 118-119 0.562283057975236 1101 120-121 0.5288518405380245 1101 122-123 0.5268922742478779 1101 124-125 0.5628105508419949 1102 1 -0.0035603261615619886 1102 2 0.11446280164586398 1102 3 0.06721150479122429 1102 4 0.061079862795970996 1102 5 -0.12186583977531029 1102 6 -0.0050124733706127245 1102 7 0.005105187161937863 1102 8 -0.05968920964715352 1102 9 -0.009120461246610034 1102 10-11 0.11885123851951107 1102 12-13 0.13081674166669188 1102 14-15 0.11464510428726982 1102 16-17 0.16247071908572508 1102 18-19 0.010999133246855308 1102 20-21 0.029818782698399104 1102 22-23 0.11484816505301865 1102 24-25 0.10123599596329313 1102 26-27 -0.07461949031944926 1102 28-29 0.11074474639827514 1102 30-31 0.058983128844168675 1102 32-33 0.1618062399125222 1102 34-35 0.06464522722615129 1102 36-37 0.2402850557259697 1102 38-39 0.20739393859231114 1102 40-41 0.08738836278473627 1102 42-43 0.046199851801986824 1102 44-45 0.16162680268227092 1102 46-47 0.13853306441811952 1102 48-49 0.19819085278103188 1102 50-51 0.2463726444006653 1102 52-53 0.2214990862683166 1102 54-55 0.10064795016546668 1102 56-57 0.1234546589979928 1102 58-59 0.17922247801921998 1102 60-61 0.18652435455041427 1102 62-63 0.1639744555380105 1102 64-65 0.11716067006452136 1102 66-67 0.22611997170012899 1102 68-69 0.1937229023449376 1102 70-71 0.14483197657678915 1102 72-73 0.06422540899761486 1102 74-75 0.13293894142198326 1102 76-77 0.12106553045247637 1102 78-79 0.13827365065566966 1102 80-81 0.06246794509632991 1102 82-83 0.17608547513577832 1102 84-85 0.1844209999528843 1102 86-87 0.17216152297236675 1102 88-89 0.14067408702673134 1102 90-91 0.13737591275089756 1102 92-93 0.1742487134389421 1102 94-95 0.06060121487391257 1102 96-97 0.11118107900092156 1102 98-99 0.15843833130807639 1102 100-101 0.06528583588520576 1102 102-103 0.12594238405776537 1102 104-105 0.20217728079068564 1102 106-107 0.14142243293977685 1102 108-109 0.15972446652656913 1102 110-111 0.05468632503608717 1102 112-113 -0.0273328833640889 1102 114-115 0.05182536627165035 1102 116-117 -0.01614916064229277 1102 118-119 0.08889722771318986 1102 120-121 0.07498929510190067 1102 122-123 0.21381363650834828 1102 124-125 0.3542951563867831 1103 1 0.09407101607272494 1103 2 0.09012998918903747 1103 3 0.11547112233074586 1103 4 0.03595192058563157 1103 5 -0.010195391893148553 1103 6 0.08153386980001898 1103 7 0.07165432403170513 1103 8 0.07952526969635443 1103 9 0.007589543573288893 1103 10-11 0.08881244908153008 1103 12-13 0.07960877379116482 1103 14-15 0.004942525795073038 1103 16-17 0.04944409912397418 1103 18-19 0.10211862374980996 1103 20-21 0.04267670621243269 1103 22-23 0.09969695224990005 1103 24-25 0.07566213639118757 1103 26-27 0.1797544908246138 1103 28-29 0.08423607211596362 1103 30-31 0.006344157662248051 1103 32-33 0.03080976427143156 1103 34-35 0.06451782226565683 1103 36-37 0.07069216829976455 1103 38-39 -2.911132753169454E-4 1103 40-41 0.11415338198757752 1103 42-43 0.13579393652782557 1103 44-45 0.06218492850925372 1103 46-47 0.14371560656152838 1103 48-49 -0.02657605754122727 1103 50-51 0.010827734919587328 1103 52-53 0.07650494996649826 1103 54-55 0.03119919919457459 1103 56-57 0.16195474956295897 1103 58-59 0.12294402296554807 1103 60-61 0.12338675412306799 1103 62-63 0.014546089360806036 1103 64-65 0.009046048580053423 1103 66-67 0.08085452423241435 1103 68-69 -0.013648625869883801 1103 70-71 0.03927389679979143 1103 72-73 0.007997992678113519 1103 74-75 0.06459654135812798 1103 76-77 0.08630584997798252 1103 78-79 0.05373163311369922 1103 80-81 -0.04068862792513528 1103 82-83 0.1341254956433744 1103 84-85 0.14618876680487602 1103 86-87 0.06841589388351821 1103 88-89 0.0381123263208778 1103 90-91 0.02536200872520311 1103 92-93 0.04093208601710785 1103 94-95 0.10624749572357217 1103 96-97 0.09178827876269224 1103 98-99 0.15707678673015124 1103 100-101 0.14528387108580887 1103 102-103 0.10409742207970396 1103 104-105 0.06814073859202097 1103 106-107 0.06401586068581366 1103 108-109 0.08116472588008605 1103 110-111 0.09938896492826643 1103 112-113 0.16744013625626764 1103 114-115 0.10784095265681515 1103 116-117 0.0396484558104504 1103 118-119 0.00466758257438471 1103 120-121 0.07036280653611016 1103 122-123 0.07559038310755994 1103 124-125 0.17215134535136656 1104 1 0.012463049755808697 1104 2 0.08453619115388022 1104 3 0.044214615539921454 1104 4 -0.01571032777513892 1104 5 0.0012669559723264001 1104 6 -0.049260608431474395 1104 7 -0.010111810210531758 1104 8 -0.029371490460178507 1104 9 -0.057439750908656606 1104 10-11 0.05034756904410642 1104 12-13 0.11126055188081807 1104 14-15 0.04794823617697119 1104 16-17 0.02812261724645282 1104 18-19 0.08814192567233192 1104 20-21 0.16238197921919095 1104 22-23 0.09016603524721489 1104 24-25 0.07314110302741028 1104 26-27 0.09082401788024441 1104 28-29 0.10132340605820644 1104 30-31 0.08062850349308093 1104 32-33 0.11731573826654085 1104 34-35 0.12422361507309176 1104 36-37 0.007034776362615958 1104 38-39 -0.08456417200537913 1104 40-41 -0.0633696934503476 1104 42-43 0.05068325610871227 1104 44-45 0.0010155254632238098 1104 46-47 -0.12562914753561216 1104 48-49 -0.16925652120345092 1104 50-51 -0.1969815279287772 1104 52-53 -0.209506470797308 1104 54-55 -0.23214779747127068 1104 56-57 -0.1627614021297319 1104 58-59 -0.17466897799237557 1104 60-61 -0.10864039432458128 1104 62-63 -0.12319144099183887 1104 64-65 -0.06008707237756994 1104 66-67 -0.09815443861668882 1104 68-69 -0.07667817282645473 1104 70-71 -0.04918058722376628 1104 72-73 0.017271979623693312 1104 74-75 -0.11819285767801091 1104 76-77 0.01506827878385053 1104 78-79 -0.07720987445682681 1104 80-81 -0.1451956712245348 1104 82-83 -0.17185629648090384 1104 84-85 -0.15875045364259677 1104 86-87 -0.13794312502980688 1104 88-89 -0.20470811224239327 1104 90-91 -0.2065181103834064 1104 92-93 -0.2453195569243789 1104 94-95 -0.18975969237745005 1104 96-97 -0.1786840715532776 1104 98-99 -0.22201831451959464 1104 100-101 -0.12389751707907237 1104 102-103 -0.1755850539537711 1104 104-105 -0.137183505654896 1104 106-107 -0.03352622460605659 1104 108-109 -0.07390333088214618 1104 110-111 -0.14154830997946988 1104 112-113 -0.11889913023069454 1104 114-115 -0.016167898806422443 1104 116-117 -0.12175077339937701 1104 118-119 -0.049475971525730245 1104 120-121 -0.01659205248651574 1104 122-123 -0.02609228599474278 1104 124-125 0.09207716236667096 1105 1 -0.04517657089751026 1105 2 0.04519214478929001 1105 3 -0.009722277095605136 1105 4 -0.048518254247454706 1105 5 -0.1584025792109074 1105 6 -0.036684504930711626 1105 7 -0.06683379826813507 1105 8 -0.012442852127428239 1105 9 -0.0142846753905701 1105 10-11 -0.007351324528073633 1105 12-13 0.040790905469393124 1105 14-15 -0.03798359264869333 1105 16-17 -0.08777130618171469 1105 18-19 -0.027139510927597144 1105 20-21 -0.054635934374068995 1105 22-23 0.024486518797147028 1105 24-25 -0.03496977391908729 1105 26-27 -0.03754575732173038 1105 28-29 -0.0032113381169480704 1105 30-31 0.016934263942673056 1105 32-33 -0.09934507428467754 1105 34-35 -0.058722614876558055 1105 36-37 0.10939325312178028 1105 38-39 0.018580951516462108 1105 40-41 -0.07505597140374931 1105 42-43 -0.02772676613692937 1105 44-45 -0.10939309179470769 1105 46-47 0.026228802353159608 1105 48-49 -0.06449127123271836 1105 50-51 -0.15113721812898717 1105 52-53 -0.24175240218707472 1105 54-55 -0.25345165533335035 1105 56-57 -0.11269847038871461 1105 58-59 -0.2733935125901965 1105 60-61 -0.0632805125777125 1105 62-63 -0.08247533632025039 1105 64-65 -0.08684696583266316 1105 66-67 -0.10148543030383905 1105 68-69 -0.07369989159474954 1105 70-71 -0.10027457504720161 1105 72-73 -0.19094939375179365 1105 74-75 -0.25877139063668153 1105 76-77 -0.2021501382633346 1105 78-79 -0.14886058865891272 1105 80-81 -0.16181989555423826 1105 82-83 -0.08667379443195955 1105 84-85 -0.22305425296352155 1105 86-87 -0.19836857286963294 1105 88-89 -0.13596835694441722 1105 90-91 -0.12402045202958334 1105 92-93 -0.2573080535875292 1105 94-95 -0.22279373405875447 1105 96-97 -0.13620807342658736 1105 98-99 -0.3091363334869257 1105 100-101 -0.06486022636369171 1105 102-103 -0.1304864121085565 1105 104-105 -0.07846160540902503 1105 106-107 -0.09949405624168861 1105 108-109 -0.1480757053113706 1105 110-111 -0.2004597372128103 1105 112-113 -0.22843621605202458 1105 114-115 -0.139772424037659 1105 116-117 -0.2597491049764784 1105 118-119 -0.2563911453929606 1105 120-121 -0.5934814682457983 1105 122-123 -0.5917432636320257 1105 124-125 -0.6250131959051934 1106 1 0.005676469834902775 1106 2 0.008391274710454866 1106 3 -0.039558824949935456 1106 4 0.028861683961046936 1106 5 -0.21855700116505972 1106 6 0.0051340847691889735 1106 7 -0.0735255109376709 1106 8 -0.0757294442808174 1106 9 0.056745731933538934 1106 10-11 0.049984002262633 1106 12-13 0.045384802422972825 1106 14-15 -0.014961573747761747 1106 16-17 0.08164985286324367 1106 18-19 -0.03857704358395608 1106 20-21 0.07704583940543586 1106 22-23 0.020801320360106956 1106 24-25 -0.001964139988281488 1106 26-27 -0.012951973955452445 1106 28-29 -0.009175382052518444 1106 30-31 -0.029978716815634243 1106 32-33 0.11281899123248706 1106 34-35 0.08629785923689326 1106 36-37 0.10530623955945373 1106 38-39 0.11317788693071407 1106 40-41 0.09972508904741773 1106 42-43 0.1684214880260484 1106 44-45 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2216 7 -0.13337908151274291 2216 8 -0.0998301923599243 2216 9 -0.15010574575806146 2216 10-11 -0.16507063720379023 2216 12-13 -0.09400015054145427 2216 14-15 -0.10629167723324429 2216 16-17 -0.20540106431216287 2216 18-19 -0.2676301043355096 2216 20-21 -0.2061433818825904 2216 22-23 -0.2364024714522941 2216 24-25 -0.1893061779788212 2216 26-27 -0.21779203855241036 2216 28-29 -0.39842093014901536 2216 30-31 -0.3438424404101781 2216 32-33 -0.4542981160983999 2216 34-35 -0.3910732908351662 2216 36-37 -0.26412933366611924 2216 38-39 -0.29074862316799255 2216 40-41 -0.25386721145878255 2216 42-43 -0.23901367965809328 2216 44-45 -0.204849812498189 2216 46-47 -0.32741132944879325 2216 48-49 -0.23389467295950084 2216 50-51 -0.2790104160712019 2216 52-53 -0.20684906027963024 2216 54-55 -0.2976089253778156 2216 56-57 -0.4238949071497018 2216 58-59 -0.39290321827900954 2216 60-61 -0.39975747714471765 2216 62-63 -0.5283980248010423 2216 64-65 -0.5109119146871279 2216 66-67 -0.36391788429279615 2216 68-69 -0.514684368474569 2216 70-71 -0.37877787009615815 2216 72-73 -0.38290157316995277 2216 74-75 -0.3474766258264097 2216 76-77 -0.42595549608271455 2216 78-79 -0.36237764215734813 2216 80-81 -0.43519305860183977 2216 82-83 -0.23939819175205912 2216 84-85 -0.23779162111171814 2216 86-87 -0.307632969392138 2216 88-89 -0.3741982775840853 2216 90-91 -0.33602395146130704 2216 92-93 -0.2891612319887571 2216 94-95 -0.2741530040633364 2216 96-97 -0.3205476180019673 2216 98-99 -0.2872349755877863 2216 100-101 -0.3927961099951318 2216 102-103 -0.3665397809347013 2216 104-105 -0.5010061458967598 2216 106-107 -0.3885891141667628 2216 108-109 -0.45337931341489934 2216 110-111 -0.516750788238717 2216 112-113 -0.34210855060168655 2216 114-115 -0.643352237999494 2216 116-117 -0.6417149431452103 2216 118-119 -0.6196752773145562 2216 120-121 -0.6550218662438425 2216 122-123 -0.5651062075414188 2216 124-125 -0.7108508412163559 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76.0 3 3.0 4 13.0 5 21.0 6 50.0 7 124.0 8 260.0 9 513.0 10 1070.0 11 1515.0 12 1941.0 13 3291.0 14 5675.0 15 5821.0 16 2254.0 17 1393.0 18 1167.0 19 1086.0 20 1089.0 21 1139.0 22 1144.0 23 1320.0 24 1627.0 25 2129.0 26 2779.0 27 3329.0 28 4540.0 29 5874.0 30 7603.0 31 9743.0 32 12477.0 33 17004.0 34 24146.0 35 39130.0 36 85699.0 37 279240.0 38 268448.0 39 3117.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.881597338437835 21.437534394598654 16.62598859008157 23.05487967688194 2 13.161997869273673 21.081656953061355 44.535564329134544 21.220780848530428 3 18.38102400200539 26.878360594096634 30.143260011280315 24.597355392617658 4 11.508428902675941 19.20849783793946 39.70746380898665 29.575609450397945 5 11.825736034111928 38.15130015334305 34.51059472509932 15.512369087445697 6 27.37795324935765 38.043115873911134 20.746756909193458 13.83217396753776 7 26.026947421194457 32.737231309143326 23.29184683837814 17.943974431284076 8 23.778028451463307 38.700256940527666 21.182803785172652 16.33891082283637 9 24.820454972739235 18.92836999435984 23.263520711913266 32.98765432098765 10-11 23.039857115999247 27.042363852854546 30.70852917215015 19.209249858996053 12-13 25.9867769630883 24.80322115685906 30.11499655323682 19.095005326815816 14-15 20.4453218023438 29.94535313655449 27.082972989910388 22.526352071191326 16-17 19.00996427899981 30.7426834618036 30.964028326126463 19.283323933070125 18-19 20.273422322491697 27.79006078836874 33.18944663783919 18.747070251300368 20-21 20.944158561783556 28.16112376013933 31.017715115473827 19.877002562603288 22-23 19.702111365754273 30.109726345289868 31.376257522574775 18.811904766381087 24-25 20.358463370307703 28.380146644106034 32.90286394685718 18.358526038729085 26-27 18.43234943911763 30.103340226859686 32.10835370057028 19.355956633452404 28-29 18.690229993106474 30.001065363163505 30.421695807482607 20.887008836247414 30-31 19.52140126590211 28.395939086294415 32.02650874224478 20.056150905558688 32-33 18.721125524848027 29.368490317728895 32.93726891019615 18.97311524722692 34-35 19.003634768440183 29.78598734097888 32.457103465563705 18.75327442501723 36-37 18.455160744500844 29.469198470890518 31.903678636335155 20.171962148273483 38-39 19.966597731403148 29.77658707777151 30.896095757347876 19.36071943347747 40-41 21.525600050134738 27.38403208623175 32.54772200288274 18.542645860750767 42-43 19.079839568841262 29.91383092059911 32.39330701259635 18.61302249796328 44-45 18.72726703014351 30.266842138246535 30.990662405214014 20.01522842639594 46-47 20.631823024378015 28.61051576110798 30.58594973992605 20.171711474587955 48-49 20.01422573165382 29.289026759415933 30.24321614338535 20.4535313655449 50-51 18.946982515510435 30.685153850974494 30.380710659898476 19.987152973616595 52-53 18.93206743122141 29.594535313655452 31.173591527229426 20.299805727893713 54-55 19.469135802469136 28.880428652002255 31.006266842138245 20.64416870339036 56-57 20.03603434229492 29.5368176975622 28.890768941530364 21.53637901861252 58-59 19.9167136679827 30.17534624302814 28.57962022936642 21.328319859622734 60-61 19.3949363915523 28.1168766058783 31.966096384032088 20.52209061853732 62-63 20.185247853606565 27.966409726139 31.313216770069563 20.53512565018487 64-65 20.677006956194774 29.29228551732782 29.4235131917027 20.607194334774707 66-67 20.373754465125025 30.91583631008335 28.636397819138935 20.07401140565269 68-69 21.248166948674562 28.23757598546093 31.11925800589083 19.39499905997368 70-71 21.943222410227488 28.020241900106534 30.26959954878737 19.76693614087861 72-73 20.73334586701761 29.92648994171837 29.298991038415746 20.04117315284828 74-75 20.02845146330764 30.261891332957326 30.82408974117942 18.88556746255562 76-77 20.381462680955067 28.790624804161187 31.344237638653883 19.483674876229866 78-79 19.91326690480667 28.494516513129035 30.8702763677383 20.721940214326 80-81 20.41868772325625 29.44237638653882 30.3477470702513 19.791188819953625 82-83 19.98527292097512 29.229617095945354 31.39775647051451 19.38735351256502 84-85 19.89446637839193 29.10785235319922 31.3270038227737 19.670677445635146 86-87 18.57072131353011 31.06298176348938 29.287272043617225 21.07902487936329 88-89 19.73077646174093 29.1779783167262 30.491257755217145 20.59998746631572 90-91 19.950529145126833 29.205163669316097 31.44363524926881 19.40067193628826 92-93 19.06078836874099 30.44507112865827 31.163564579808234 19.330575922792505 94-95 19.101991806663836 30.645797076745 30.399570843154077 19.852640273437082 96-97 20.79031146205427 29.720624177476967 29.75339976186 19.73566459860876 98-99 20.600195904063412 28.67989113098562 31.481770456338648 19.23814250861232 100-101 19.96303814190521 29.013867903658525 31.30985229670508 19.71324165773119 102-103 19.20722994856163 30.255574996741235 30.4769830844973 20.060211970199838 104-105 19.618139441899 29.515103772041805 30.47282599499518 20.393930791064015 106-107 19.9060416733256 28.610034714192334 30.335215382793066 21.148708229689 108-109 19.22086760778042 29.684877714956087 30.133020157949687 20.961234519313805 110-111 19.068055792313015 29.79496525514655 30.57722582650636 20.55975312603407 112-113 19.813547424760902 28.583649832662733 30.226939420148156 21.375863322428206 114-115 19.68916138501735 29.339585452416898 28.593856052102662 22.377397110463086 116-117 19.953900329202785 29.483860415009843 27.728676238803313 22.83356301698406 118-119 19.704780490495043 28.468052145336138 29.72017191265995 22.106995451508872 120-121 19.50046688266518 27.847576910302124 30.721694063383236 21.930262143649458 122-123 20.37631581750989 28.989942225294403 29.42580628584285 21.20793567135286 124-125 20.894806833977366 29.346345180782542 28.428551195294276 21.330296789945816 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 22.0 1 17.0 2 35.5 3 52.0 4 31.5 5 18.0 6 53.5 7 135.0 8 225.0 9 265.0 10 221.5 11 200.5 12 246.5 13 292.0 14 357.5 15 446.5 16 553.5 17 715.5 18 856.0 19 1031.5 20 1291.5 21 1619.0 22 2000.0 23 2505.5 24 3195.5 25 4007.5 26 5099.0 27 6474.0 28 7981.0 29 9758.0 30 12280.5 31 16198.0 32 21575.5 33 28841.0 34 38215.5 35 47523.0 36 54297.5 37 55899.0 38 50530.0 39 41589.0 40 34480.5 41 29975.5 42 26448.5 43 24193.0 44 23229.5 45 22584.5 46 21854.0 47 21546.0 48 21507.0 49 20454.0 50 18996.0 51 17383.5 52 15504.5 53 14054.5 54 12740.5 55 11028.5 56 9180.5 57 7775.5 58 6531.5 59 5253.5 60 4059.0 61 3160.5 62 2466.0 63 1777.5 64 1283.5 65 1005.5 66 718.0 67 498.5 68 373.0 69 266.5 70 197.5 71 150.0 72 134.0 73 117.5 74 89.5 75 64.0 76 43.0 77 31.5 78 17.5 79 8.0 80 5.5 81 3.0 82 2.0 83 1.0 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 3.0 97 3.0 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015416431660086482 2 0.0 3 0.0 4 0.0 5 0.11831797957009463 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.003572100018800526 22-23 1.8800526414739614E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 8.146894779720499E-4 92-93 0.0 94-95 8.146894779720499E-4 96-97 0.0 98-99 0.0013160368490317729 100-101 6.266842138246538E-5 102-103 2.5067368552986153E-4 104-105 0.0024440684339161497 106-107 0.006204173716864072 108-109 1.2533684276493077E-4 110-111 0.0042614526540076456 112-113 0.007520210565895844 114-115 0.005076142131979695 116-117 0.0016920473773265653 118-119 3.7601052829479227E-4 120-121 6.266842138246537E-4 122-123 0.0011907000062668421 124-125 0.0016293789559440998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 797850.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.2563984218205 #Duplication Level Percentage of deduplicated Percentage of total 1 90.74831495741492 71.01636291416315 2 6.551140270754063 10.253372862907261 3 1.2127267212532582 2.847108764255491 4 0.47979408053730327 1.5018782690783294 5 0.25431912314818067 0.995104931368603 6 0.15660886829542753 0.7353387596230435 7 0.10369237142162202 0.5680214071891693 8 0.07547988855322157 0.47254273851644124 9 0.05928793085649185 0.4175693944839875 >10 0.3030586127300781 4.443780121598165 >50 0.0334755675744798 1.819331453569456 >100 0.02033988515553776 3.3790967345252976 >500 8.007828661352493E-4 0.47801916968912656 >1k 9.609394393622992E-4 1.072472479032474 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1720 0.21557936955568088 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1635 0.20492573792066177 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1495 0.1873785799335715 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1292 0.16193520085229055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1240 0.15541768502851414 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1175 0.14727079024879364 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 987 0.12370746380898665 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 1.880052641473961E-4 0.0 28-29 0.0 0.0 0.0 4.386789496772576E-4 0.0 30-31 0.0 0.0 0.0 0.0010026947421194461 0.0 32-33 0.0 0.0 0.0 0.003446763176035595 0.0 34-35 0.0 0.0 0.0 0.006454847402393934 0.0 36-37 0.0 0.0 0.0 0.010528294792254183 0.0 38-39 0.0 0.0 0.0 0.021871279062480417 0.0 40-41 0.0 0.0 0.0 0.033966284389296234 0.0 42-43 0.0 0.0 0.0 0.04637463182302438 0.0 44-45 0.0 0.0 0.0 0.06348311086043743 0.0 46-47 0.0 0.0 0.0 0.08153161621858745 0.0 48-49 0.0 0.0 0.0 0.10258820580309583 0.0 50-51 0.0 0.0 0.0 0.1270915585636398 0.0 52-53 0.0 0.0 0.0 0.1514695744814188 0.0 54-55 0.0 0.0 0.0 0.17973303252491069 0.0 56-57 0.0 0.0 0.0 0.21670740114056525 0.0 58-59 0.0 0.0 0.0 0.25744187503916777 0.0 60-61 0.0 0.0 0.0 0.30857930688725954 0.0 62-63 0.0 0.0 0.0 0.35595663345240336 0.0 64-65 0.0 0.0 0.0 0.40715673372187755 0.0 66-67 0.0 0.0 0.0 0.4655637024503353 0.0 68-69 0.0 0.0 0.0 0.5498527292097513 0.0 70-71 0.0 0.0 0.0 0.63802719809488 0.0 72-73 0.0 0.0 0.0 0.7208748511624992 0.0 74-75 0.0 0.0 0.0 0.8041611831797957 0.0 76-77 0.0 0.0 0.0 0.9016105784295294 0.0 78-79 0.0 0.0 0.0 1.012784357962023 0.0 80-81 0.0 0.0 0.0 1.133170395437739 0.0 82-83 0.0 0.0 0.0 1.2622673434856178 0.0 84-85 0.0 0.0 0.0 1.392241649432851 0.0 86-87 0.0 0.0 0.0 1.525976060663032 0.0 88-89 0.0 0.0 0.0 1.6707401140565268 0.0 90-91 0.0 0.0 0.0 1.8291032148900168 0.0 92-93 0.0 0.0 0.0 1.9949238578680204 0.0 94-95 0.0 0.0 0.0 2.164504606128972 0.0 96-97 0.0 0.0 0.0 2.339913517578492 0.0 98-99 0.0 0.0 0.0 2.5460299555054204 0.0 100-101 0.0 0.0 0.0 2.763364040859811 0.0 102-103 0.0 0.0 0.0 2.9991853105220283 0.0 104-105 0.0 0.0 0.0 3.231873159115122 0.0 106-107 0.0 0.0 0.0 3.464561007708216 0.0 108-109 0.0 0.0 0.0 3.7099078774205676 0.0 110-111 0.0 0.0 0.0 3.9916024315347496 0.0 112-113 0.0 0.0 0.0 4.303064485805603 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3330 0.0 45.4349 1 GGTATCA 1980 0.0 37.90578 1 ATCAACG 3985 0.0 37.5185 3 TCAACGC 3985 0.0 37.5185 4 TATCAAC 4025 0.0 37.145645 2 CAACGCA 4070 0.0 36.58859 5 AACGCAG 4135 0.0 35.970554 6 ACGCAGA 4705 0.0 31.612804 7 CGCAGAG 4730 0.0 31.445719 8 TAGGACC 1395 0.0 29.889864 4 TACATGG 3500 0.0 29.783113 2 CGAAATG 140 3.0661467E-6 29.783112 5 GTACATG 3615 0.0 29.000431 1 GCAGAGT 5160 0.0 28.59464 9 CCTACCG 105 5.8657554E-4 28.36487 3 ACATGGG 3500 0.0 27.911032 3 GTACCTG 750 0.0 27.003355 1 ACACCGT 90 0.00953092 26.442354 6 TATCGTG 90 0.00953092 26.442354 7 TACCTGG 760 0.0 25.864283 2 >>END_MODULE