FastQCFastQC Report
Fri 27 May 2016
SRR939981_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939981_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784405
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44550.5679464052370906No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT22250.28365448970876017No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14730.18778564644539492No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14540.18536342833102798No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14190.18090144759403626No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10150.12939744137276024No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8990.11460916235873049No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8640.11014718162173877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCG650.03471.7834108-109
TTGCGCG108.7386876E-43054.4683110-111
CATCGCG201.3694771E-62507.3994108-109
CCGCGTA100.00129779112507.3994108-109
CCGCTCC1700.02064.917108-109
CTCGACG200.00349471931527.2341110-111
CGTCGCT200.00349471931527.2341110-111
CGTCGCG200.00349471931527.2341110-111
CCGCGCC2200.01481.6449108-109
CCGCGCG950.01319.6838108-109
CGTGACG250.00545990841221.7874110-111
CGCTAGC250.00545990841221.7874110-111
CGTCGGC501.1800523E-51221.7874110-111
CGGCGCG300.0022715751018.1561110-111
CGCCGCC1201.2187229E-101018.1561110-111
GGACGCC502.1379095E-51002.9597108-109
CGTCGCC806.899427E-8940.2748108-109
CCGCACC603.693215E-5835.79974108-109
TTGTTCG1001.6833837E-7752.2198108-109
CTCCGCC1251.8569517E-7733.0723110-111
Produced by FastQC (version 0.11.5)