##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939981_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 784405 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.608463740032253 33.0 31.0 34.0 30.0 34.0 2 31.799626468469732 34.0 31.0 34.0 30.0 34.0 3 31.877966101694916 34.0 31.0 34.0 30.0 34.0 4 35.16904915190495 37.0 35.0 37.0 33.0 37.0 5 35.19810812016752 37.0 35.0 37.0 33.0 37.0 6 35.2668264480721 37.0 37.0 37.0 33.0 37.0 7 35.180832605605524 37.0 37.0 37.0 33.0 37.0 8 35.18975274252459 37.0 37.0 37.0 33.0 37.0 9 36.85609219727054 39.0 38.0 39.0 34.0 39.0 10-11 36.824037327656 39.0 38.0 39.0 34.0 39.0 12-13 36.75935518004093 39.0 38.0 39.0 33.0 39.0 14-15 38.23957840656294 41.0 39.0 41.0 33.5 41.0 16-17 38.25025018963418 41.0 39.0 41.0 33.5 41.0 18-19 38.26415117190737 41.0 39.0 41.0 33.5 41.0 20-21 38.30523262855285 41.0 39.0 41.0 34.0 41.0 22-23 38.286685449480814 41.0 39.0 41.0 34.0 41.0 24-25 38.29934408883166 41.0 39.0 41.0 34.0 41.0 26-27 38.243830674205284 41.0 39.0 41.0 33.0 41.0 28-29 38.19002556077537 41.0 39.0 41.0 33.0 41.0 30-31 38.11778545521765 41.0 39.0 41.0 33.0 41.0 32-33 38.00597841676175 40.0 38.0 41.0 33.0 41.0 34-35 37.90626207125146 40.0 38.0 41.0 33.0 41.0 36-37 38.090629203026495 41.0 39.0 41.0 33.0 41.0 38-39 38.06035020174527 41.0 39.0 41.0 33.0 41.0 40-41 38.010352432735644 41.0 39.0 41.0 33.0 41.0 42-43 37.92893849478267 41.0 38.0 41.0 33.0 41.0 44-45 37.82444336790306 41.0 38.0 41.0 33.0 41.0 46-47 37.71682804163665 40.0 38.0 41.0 32.0 41.0 48-49 37.61425475360305 40.0 38.0 41.0 32.0 41.0 50-51 37.489023527387005 40.0 38.0 41.0 32.0 41.0 52-53 37.39117037754731 40.0 38.0 41.0 31.5 41.0 54-55 37.23591448295204 40.0 37.5 41.0 31.0 41.0 56-57 37.025998049476996 40.0 37.0 41.0 30.5 41.0 58-59 36.85212485896953 40.0 37.0 41.0 30.5 41.0 60-61 36.61524531332666 40.0 36.0 41.0 30.0 41.0 62-63 36.38633167815095 39.5 36.0 41.0 30.0 41.0 64-65 36.10241010702379 39.0 35.0 41.0 30.0 41.0 66-67 35.77856081998458 39.0 35.0 41.0 29.5 41.0 68-69 35.38591798879405 38.5 35.0 40.5 29.0 41.0 70-71 34.96784632938342 37.0 35.0 40.0 28.5 41.0 72-73 34.581252031794804 37.0 34.5 39.5 28.0 41.0 74-75 34.16923464281844 36.5 34.0 39.0 28.0 41.0 76-77 33.715483710583186 36.0 34.0 39.0 27.0 40.5 78-79 33.24145435074993 35.5 34.0 37.5 26.0 39.5 80-81 32.76451131749543 35.0 34.0 37.0 26.0 39.0 82-83 32.538317578291824 35.0 34.0 37.0 26.0 39.0 84-85 32.10558639988271 35.0 34.0 36.0 24.5 38.0 86-87 31.432248009637878 35.0 34.0 36.0 7.5 37.0 88-89 16.073441015801787 18.0 16.5 18.5 2.0 19.0 90-91 2.7413772222257635 2.0 2.0 2.0 2.0 2.0 92-93 2.2882624409584333 2.0 2.0 2.0 2.0 2.0 94-95 2.1003072392450326 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29271162209965595 1101 2 0.3586772351716192 1101 3 0.34676757684836446 1101 4 0.3335541777527027 1101 5 0.24495833556474622 1101 6 0.278913317360292 1101 7 0.2405779451212453 1101 8 0.3105001020613898 1101 9 0.39069790933894666 1101 10-11 0.248626816071301 1101 12-13 0.37501540793228116 1101 14-15 0.17780469201860427 1101 16-17 0.38563208908257707 1101 18-19 0.40987710232131747 1101 20-21 0.3815763217407948 1101 22-23 0.5227761023003055 1101 24-25 0.3968771975880969 1101 26-27 0.4015433016078589 1101 28-29 0.47276818033228807 1101 30-31 0.46119011161905377 1101 32-33 0.5244686443631466 1101 34-35 0.5106338553587264 1101 36-37 0.5156280299467184 1101 38-39 0.5194603306204044 1101 40-41 0.502026515190245 1101 42-43 0.5645572993090937 1101 44-45 0.5515912655299147 1101 46-47 0.5050801594875836 1101 48-49 0.5586964216294987 1101 50-51 0.599707671959294 1101 52-53 0.5536127040068379 1101 54-55 0.4622124226253419 1101 56-57 0.44516082770849863 1101 58-59 0.4610026896033901 1101 60-61 0.40945654204744386 1101 62-63 0.48124663703192283 1101 64-65 0.5310889056538741 1101 66-67 0.4473805087183891 1101 68-69 0.46270068971497835 1101 70-71 0.4708120012680439 1101 72-73 0.5097156200062685 1101 74-75 0.5182480612081548 1101 76-77 0.5025472594848424 1101 78-79 0.5075405935060999 1101 80-81 0.49055563701625715 1101 82-83 0.4396926531947827 1101 84-85 0.42075190227038206 1101 86-87 0.512663172928594 1101 88-89 0.8883186329191588 1101 90-91 0.0672292807005368 1101 92-93 -0.18892774001669554 1101 94-95 -0.032838790936205786 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.12861052919680915 1102 2 0.09420481175117956 1102 3 -0.03380914574226779 1102 4 0.1251311078569799 1102 5 0.11818568536744323 1102 6 0.07575166918935139 1102 7 0.09541925505321558 1102 8 -8.919011397807708E-4 1102 9 0.0761576512779456 1102 10-11 0.08331491313808215 1102 12-13 0.17565679872016204 1102 14-15 -0.003482454784489164 1102 16-17 -0.06854873219195667 1102 18-19 -0.03569438282326587 1102 20-21 0.0013455930206589528 1102 22-23 0.02690033270162928 1102 24-25 0.10394150780363987 1102 26-27 0.04926036212329876 1102 28-29 0.04382672315657743 1102 30-31 0.0017305069201540846 1102 32-33 -0.0024242149351607623 1102 34-35 0.042201972333593574 1102 36-37 0.06325406555143331 1102 38-39 0.11817027366576838 1102 40-41 0.1614689235390685 1102 42-43 0.09426234056792993 1102 44-45 0.13574301009136747 1102 46-47 0.13548057815256698 1102 48-49 0.07980080760818709 1102 50-51 0.041114416070165305 1102 52-53 0.061438283412229566 1102 54-55 0.08366825900868236 1102 56-57 0.00573951795841765 1102 58-59 0.08103197300336973 1102 60-61 0.03525693706744448 1102 62-63 0.08441481650890381 1102 64-65 -0.005236317009924107 1102 66-67 0.06108682647552399 1102 68-69 -0.10504755571992774 1102 70-71 0.05411426267075825 1102 72-73 -0.04202017791677548 1102 74-75 -0.11368266726395859 1102 76-77 0.010961069807450485 1102 78-79 -0.11309800287489935 1102 80-81 -0.03199101015018613 1102 82-83 0.0733227613581704 1102 84-85 0.1306664248360505 1102 86-87 0.18255939130239796 1102 88-89 1.3604311962583608 1102 90-91 0.07191983320769024 1102 92-93 -0.11357961727113297 1102 94-95 0.026503084904493157 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.10877061362752016 1103 2 0.2704899088385986 1103 3 0.35939469456674544 1103 4 0.3105559851076549 1103 5 0.29852023642153824 1103 6 0.1949893657480004 1103 7 0.11664431395105623 1103 8 0.16760646880739927 1103 9 0.1806993965626873 1103 10-11 0.26070315686231993 1103 12-13 0.3023015102885651 1103 14-15 0.22476861297895567 1103 16-17 0.2811058874772101 1103 18-19 0.38916184012880706 1103 20-21 0.43960577549275115 1103 22-23 0.3469560076389371 1103 24-25 0.3743381957756924 1103 26-27 0.3172748518411481 1103 28-29 0.22845092946116807 1103 30-31 0.2747872017019617 1103 32-33 0.17450263204692362 1103 34-35 0.1621105340940332 1103 36-37 0.21781105593448302 1103 38-39 0.1991799763418669 1103 40-41 0.1777083904701513 1103 42-43 0.24234481452793233 1103 44-45 0.25167369301515663 1103 46-47 0.13592013841438444 1103 48-49 0.11414676054005923 1103 50-51 0.13892122468473644 1103 52-53 0.18158704645649237 1103 54-55 0.15792644546473156 1103 56-57 0.10085760883537631 1103 58-59 0.20711217111686864 1103 60-61 0.24789573014091815 1103 62-63 0.19897829313537585 1103 64-65 0.18600636473631482 1103 66-67 0.0912555415927514 1103 68-69 0.15363669120052492 1103 70-71 0.16826405930842014 1103 72-73 0.30611721074750164 1103 74-75 0.29786502967481 1103 76-77 0.27585739100751994 1103 78-79 0.40354584332187216 1103 80-81 0.2759332868162403 1103 82-83 0.2657122627019177 1103 84-85 0.25299600526021493 1103 86-87 0.31098134974353187 1103 88-89 0.9543013727213854 1103 90-91 -0.5202551853735371 1103 92-93 -0.29689808292679754 1103 94-95 -0.10373777147374064 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.09279116026202772 1104 2 0.07058725432622381 1104 3 0.1518648087943575 1104 4 0.2215579909908456 1104 5 -0.07912993157412984 1104 6 0.14115526667734457 1104 7 0.08013739715313761 1104 8 0.1206596809332865 1104 9 0.15094297258612954 1104 10-11 0.188591223705167 1104 12-13 0.24412303464971075 1104 14-15 0.2372253756203122 1104 16-17 0.27011323871278137 1104 18-19 0.26854936559550424 1104 20-21 0.2771559717170362 1104 22-23 0.05057359556244023 1104 24-25 0.007867425763457447 1104 26-27 0.08699576594516145 1104 28-29 0.2140350692234989 1104 30-31 0.2183244599403551 1104 32-33 0.13664247143472608 1104 34-35 0.19391766107327868 1104 36-37 0.10472830991106008 1104 38-39 0.1529915868749896 1104 40-41 0.0989658771480535 1104 42-43 0.07779712899945679 1104 44-45 0.03793587004712862 1104 46-47 0.050556480677336424 1104 48-49 0.19229600108013045 1104 50-51 -0.07723332975521657 1104 52-53 0.0028582149719085237 1104 54-55 0.16095374859370537 1104 56-57 0.07544713405201975 1104 58-59 -0.025411443813325718 1104 60-61 -0.010531770667022045 1104 62-63 0.09026272674268654 1104 64-65 -0.04369163622141059 1104 66-67 -0.1974096064423705 1104 68-69 -0.22418214082662047 1104 70-71 -0.20574076393246088 1104 72-73 -0.14329498134235052 1104 74-75 -0.11503120302336356 1104 76-77 -0.1796903975278923 1104 78-79 -0.08628202500852211 1104 80-81 -0.11286679244405917 1104 82-83 -0.11298252145137155 1104 84-85 -0.1323549142143179 1104 86-87 0.06442402245117407 1104 88-89 0.8253901560521868 1104 90-91 -0.41071438401101457 1104 92-93 -0.29689808292679754 1104 94-95 -0.10373777147374064 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.0693314579397537 1105 2 -0.013661101064343484 1105 3 0.0867502747151363 1105 4 0.2727280901610598 1105 5 0.22773573371993905 1105 6 0.25210393785114604 1105 7 0.3184375446401617 1105 8 0.12827098351256438 1105 9 0.3500679234111317 1105 10-11 0.25368639156849326 1105 12-13 0.3402330074154847 1105 14-15 0.3519865332187777 1105 16-17 0.3817309936595237 1105 18-19 0.3562619952542221 1105 20-21 0.3644064703021641 1105 22-23 0.14290297920033623 1105 24-25 0.2545204879798888 1105 26-27 0.2646223184701384 1105 28-29 0.07717705928065755 1105 30-31 0.057222226066336646 1105 32-33 0.11074680293573635 1105 34-35 0.11819233932828155 1105 36-37 0.06320524436819852 1105 38-39 0.027981144307595684 1105 40-41 0.10456802975991053 1105 42-43 0.16740233160044937 1105 44-45 0.1065397405562507 1105 46-47 0.1887651892469293 1105 48-49 0.15369381657097847 1105 50-51 0.1298963236426829 1105 52-53 0.08812120818386404 1105 54-55 0.12572204511096885 1105 56-57 0.19482200779189185 1105 58-59 0.1322629698460105 1105 60-61 0.0986276140493132 1105 62-63 0.001804687636600022 1105 64-65 0.06713893900835899 1105 66-67 0.10101200898863283 1105 68-69 0.11841988875280407 1105 70-71 0.11010512959436625 1105 72-73 0.0020591014356838855 1105 74-75 0.034586485090940755 1105 76-77 -0.022717344667995576 1105 78-79 0.09063709104143669 1105 80-81 0.05170958867091002 1105 82-83 0.13229250159822925 1105 84-85 0.0516374273966278 1105 86-87 0.11417848921191265 1105 88-89 0.6386314834050317 1105 90-91 -0.7242443059537726 1105 92-93 -0.29689808292679754 1105 94-95 -0.10373777147374064 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.020426324697929488 1106 2 -0.044772060973464534 1106 3 -0.02702149837143608 1106 4 -0.09136613760436063 1106 5 -0.07833588080175957 1106 6 7.372033408046264E-4 1106 7 -0.2525024847061701 1106 8 -0.04860132443461396 1106 9 -0.05947022682316572 1106 10-11 -0.07361694066040769 1106 12-13 -0.02000359740299018 1106 14-15 0.003981018947939674 1106 16-17 -0.08572918762509119 1106 18-19 -0.02949290369025448 1106 20-21 -0.08788872660227298 1106 22-23 -0.05762241484519848 1106 24-25 0.018539131185271174 1106 26-27 -0.27267370675824765 1106 28-29 0.07613943562372327 1106 30-31 0.06306043073334422 1106 32-33 0.1286770580595089 1106 34-35 0.005751801154382008 1106 36-37 0.10449793263439489 1106 38-39 0.08566355307362983 1106 40-41 0.1834769792084856 1106 42-43 -0.06065521916396932 1106 44-45 0.07362273861560453 1106 46-47 0.02319432613337824 1106 48-49 0.08415676090761082 1106 50-51 0.10206611821834599 1106 52-53 -0.10290656433471668 1106 54-55 -0.013817998435193601 1106 56-57 -0.08649307480383328 1106 58-59 -0.0312844162002861 1106 60-61 0.058574453401796234 1106 62-63 -0.0330026678800337 1106 64-65 -0.0724173751867454 1106 66-67 0.00600547114606087 1106 68-69 0.034394559789873824 1106 70-71 0.13082432186018167 1106 72-73 0.1345132773716955 1106 74-75 -0.07105588012892383 1106 76-77 0.11874633332699602 1106 78-79 0.22727060502827356 1106 80-81 0.22920683745209658 1106 82-83 0.14429432391091268 1106 84-85 0.010097721096627765 1106 86-87 0.18021735535476324 1106 88-89 0.6248857433567494 1106 90-91 -0.31081525189971826 1106 92-93 -0.29689808292679754 1106 94-95 -0.10373777147374064 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.10373676737027537 1107 2 -0.06234996473640564 1107 3 0.04932971138820008 1107 4 0.055479340522552434 1107 5 0.03299202296118153 1107 6 0.0815549238274258 1107 7 0.038717696078137465 1107 8 0.15466986901365232 1107 9 0.008900165782343095 1107 10-11 0.10750860021995123 1107 12-13 0.030848359960913285 1107 14-15 0.039230277007341385 1107 16-17 0.009677435469086504 1107 18-19 0.017980675295270032 1107 20-21 -0.10435761323827819 1107 22-23 0.07670281566611692 1107 24-25 -0.008372144612742716 1107 26-27 -0.03873622516517372 1107 28-29 0.003120940913596826 1107 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0.02945997287891089 2216 5 -0.11937683316447334 2216 6 -0.022782152301161318 2216 7 -0.011725385849693737 2216 8 -0.09379581463707609 2216 9 -0.07329380412123498 2216 10-11 -0.10832133853808301 2216 12-13 -0.10778457026103894 2216 14-15 -0.20348517332512728 2216 16-17 -0.2532492280411276 2216 18-19 -0.17238861212015877 2216 20-21 -0.17871915882400202 2216 22-23 -0.20308662462549876 2216 24-25 -0.1748122752353254 2216 26-27 -0.27961572363337694 2216 28-29 -0.3050489706244761 2216 30-31 -0.3690721137530204 2216 32-33 -0.3773195037042356 2216 34-35 -0.4277622630515694 2216 36-37 -0.3517055577979207 2216 38-39 -0.32767481630212814 2216 40-41 -0.3122895726906876 2216 42-43 -0.34723466492794586 2216 44-45 -0.44358400569071677 2216 46-47 -0.4091923150258552 2216 48-49 -0.40540432524942105 2216 50-51 -0.5873391578940499 2216 52-53 -0.566405831674345 2216 54-55 -0.5121647239700877 2216 56-57 -0.33993430394935586 2216 58-59 -0.4196874919372462 2216 60-61 -0.39496476308257655 2216 62-63 -0.5075774375469351 2216 64-65 -0.6355141581851953 2216 66-67 -0.4939595119979643 2216 68-69 -0.4001625278612053 2216 70-71 -0.346039113598664 2216 72-73 -0.4892485625156766 2216 74-75 -0.5978452713982847 2216 76-77 -0.6165444921370238 2216 78-79 -0.5387168343955508 2216 80-81 -0.629272090182134 2216 82-83 -0.6680596228778377 2216 84-85 -0.7094612580632571 2216 86-87 -0.9610870315090665 2216 88-89 -1.2880156635427067 2216 90-91 -0.7242443059537726 2216 92-93 -0.29689808292679754 2216 94-95 -0.10373777147374064 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11835.0 3 1893.0 4 1283.0 5 984.0 6 1007.0 7 1054.0 8 1251.0 9 1377.0 10 1689.0 11 2030.0 12 2293.0 13 2716.0 14 3151.0 15 3529.0 16 3955.0 17 4853.0 18 5955.0 19 7596.0 20 8089.0 21 11055.0 22 13717.0 23 16586.0 24 25236.0 25 42251.0 26 78523.0 27 217857.0 28 301378.0 29 10835.0 30 427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.80135899184732 19.49630611737559 13.91207348244848 23.790261408328604 2 13.69341368359725 20.68398863333584 42.9762149110338 22.64638277203311 3 20.028643319258233 25.400859809699195 28.277434650254357 26.293062220788215 4 11.675101008039832 17.446715708280617 39.815660327306865 31.06252295637269 5 13.06147227887057 38.071898550780084 34.89249142622472 13.97413774412462 6 29.59402349551571 37.392800912793774 19.45041145836653 13.562764133323984 7 27.73898687540238 31.599492609047626 22.469515110179053 18.19200540537095 8 23.48289467813183 37.29705955469432 21.07202274335324 18.148023023820603 9 24.713253995066324 18.902607709027862 21.864470522242975 34.51966777366284 10-11 23.968493055506862 26.935607347540824 29.095351406204884 20.000548190747434 12-13 26.006101564402357 24.384712177248787 28.53813605239237 21.071050205956485 14-15 21.405523932152395 27.56681816153645 26.63878991082413 24.388867995487026 16-17 21.04550916143179 29.676027294660585 28.292617629342075 20.985845914565544 18-19 21.246728178400176 29.149141517360256 28.90161140002728 20.702518904212283 20-21 22.311718975096444 28.191477155380127 27.804040357681043 21.692763511842383 22-23 21.281315456331424 29.151501089371834 27.8258968354035 21.741286618893238 24-25 21.98213932853564 28.40312083681262 28.543482002281984 21.071257832369756 26-27 21.15947756579828 28.69506186217579 28.797177478470942 21.348283093554986 28-29 20.69193675360231 29.63420736429785 27.70392373715965 21.969932144940195 30-31 21.43586540116394 28.433781018733946 28.12054997099713 22.009803609104992 32-33 20.96442727051363 28.95480964261537 28.686895472040163 21.39386761483083 34-35 21.552273093792806 28.888985211074132 28.500920124757062 21.057821570376 36-37 21.173732651626363 28.444352938251804 28.724055922748942 21.657858487372888 38-39 21.398257277809297 29.158597918167274 28.233565568806927 21.209579235216502 40-41 22.118612196505634 28.28889412994563 28.5984918505109 20.994001823037845 42-43 20.834900338473112 29.27403573409145 28.32133910416175 21.569724823273692 44-45 21.096463047103278 28.963518798986236 28.037019189091332 21.902998964819147 46-47 21.915273360062724 28.624881279441105 27.943536820902466 21.516308539593705 48-49 21.581089798343196 28.569215784763426 28.140099081848334 21.70959533504504 50-51 20.564631790975323 29.30412223277516 28.68849637623422 21.442749600015297 52-53 20.974114137467254 29.25032349360343 28.294758447485673 21.48080392144364 54-55 20.729952039408765 28.458720999905662 28.71324869899566 22.098078261689917 56-57 21.520643035166785 28.532008337529717 27.762444145562558 22.18490448174094 58-59 22.044989514345268 28.65350169873981 27.443922463523307 21.857586323391615 60-61 21.28485922450775 28.148787934804087 29.225081431148446 21.341271409539715 62-63 21.24344008735289 28.20113857072467 29.07772711655785 21.47769422536459 64-65 21.22802790398825 29.112995344235877 27.975571261747774 21.6834054900281 66-67 21.31864279294497 29.254657989176508 27.452591454669463 21.974107763209055 68-69 21.549104635827444 28.37488199601862 28.516454902240753 21.559558465913184 70-71 22.267196155047454 28.22610768671796 28.016139621751517 21.490556536483066 72-73 21.674645113174954 28.39406939017472 28.478783281595604 21.45250221505472 74-75 20.954545164806447 29.43893779361427 28.46093535864764 21.14558168293165 76-77 21.41508531944595 28.121569852308436 28.703858338485862 21.759486489759755 78-79 21.319666880204995 28.25883395323192 28.52852967704718 21.892969489515906 80-81 21.770641441602233 28.563879628508232 28.284559634372552 21.380919295516986 82-83 21.39731449934504 28.387966560321775 29.063835619057937 21.150883321275245 84-85 21.231916206444637 28.527901438544422 28.870836966665138 21.3693453883458 86-87 20.755143942421757 29.42228613572142 28.076278041569697 21.746291880287128 88-89 30.38219344292817 18.253472370142113 34.26676701552094 17.09756717140878 90-91 30.362586726848566 14.32160956041686 16.175643098976003 39.140160613758574 92-93 22.468152135517105 16.94582794842871 34.63506621431689 25.95095370173729 94-95 14.936887848982213 6.599082968124967 26.58989422583119 51.87413495706162 96-97 25.705709936040016 6.959355259645897 14.297577033719861 53.03735777059423 98-99 57.40337656161402 14.064129062940134 18.59669660331803 9.935797772127819 100-101 30.554250326014078 6.9026551008663795 12.28333922886452 50.25975534425502 102-103 22.889641484596922 1.9710128389567447 36.42943600151606 38.70990967493027 104-105 41.82512009034947 4.641045703968742 39.421793771254784 14.112040434426998 106-107 4.991806203725839 1.5012143448600819 5.90888775711717 87.59809169429691 108-109 17.544505867619158 10.057045993468996 20.295127859320104 52.10332027959175 110-111 39.65044690930896 2.5734566951629563 24.06005674141841 33.71603965410967 112-113 27.1850036117957 2.7157516784227074 37.76557321322342 32.33367149655817 114-115 27.66560568094966 3.8060040826988386 22.391958437738843 46.136431798612655 116-117 18.74347596688543 3.9176462996633123 19.14070655041393 58.19817118303733 118-119 19.230874056308085 4.1336204155424126 14.469034186403567 62.16647134174593 120-121 24.472712271275853 4.080571968724927 28.748834655951455 42.69788110404777 122-123 33.55368716617054 1.5588384318196213 32.774399898663624 32.11307450334621 124-125 37.780198477513245 1.7482673763602068 18.856129982210838 41.615404163915706 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 6.0 1 8.5 2 27.0 3 57.0 4 71.5 5 85.5 6 86.5 7 69.5 8 68.0 9 68.5 10 64.5 11 59.0 12 66.0 13 79.0 14 90.0 15 118.5 16 161.0 17 242.5 18 341.5 19 460.5 20 676.0 21 1018.0 22 1476.5 23 2118.5 24 2917.0 25 3905.0 26 5207.0 27 6722.0 28 8507.0 29 10619.5 30 13053.0 31 15872.0 32 19150.0 33 22525.0 34 25666.5 35 28421.5 36 30187.5 37 30827.5 38 31288.0 39 31609.0 40 31602.0 41 31391.5 42 31467.0 43 32478.0 44 33524.0 45 33389.0 46 31909.5 47 30286.5 48 29089.0 49 27955.5 50 26481.0 51 24203.5 52 21209.0 53 18345.0 54 15931.0 55 13722.5 56 11572.0 57 9668.5 58 8074.0 59 6530.5 60 5192.0 61 4138.5 62 3235.5 63 2409.0 64 1794.5 65 1340.0 66 961.5 67 682.5 68 490.0 69 372.0 70 259.5 71 180.5 72 137.0 73 97.5 74 74.0 75 61.5 76 46.0 77 26.0 78 14.0 79 7.5 80 3.0 81 3.0 82 6.0 83 6.0 84 3.5 85 5.5 86 6.0 87 3.5 88 2.0 89 2.0 90 3.0 91 3.0 92 3.0 93 2.0 94 1.0 95 1.0 96 1.5 97 3.5 98 3.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 7.64910983484297E-4 3 0.03544087556810576 4 0.0393929156494413 5 0.041815133763808236 6 0.0 7 0.0 8 0.0 9 0.0 10-11 8.286535654413219E-4 12-13 0.002039762622624792 14-15 0.0 16-17 3.187129097851238E-4 18-19 0.007139169179186772 20-21 0.0024222181143669407 22-23 0.001019881311312396 24-25 0.0 26-27 0.0 28-29 0.0015935645489256187 30-31 0.0 32-33 0.001529821966968594 34-35 6.374258195702475E-5 36-37 1.9122774587107424E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 1.274851639140495E-4 46-47 0.0 48-49 3.824554917421485E-4 50-51 0.0 52-53 0.0 54-55 0.0014023368030545446 56-57 0.0 58-59 0.0 60-61 0.0 62-63 6.374258195702475E-5 64-65 1.274851639140495E-4 66-67 0.0 68-69 4.4619807369917326E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.187129097851238E-4 80-81 0.0 82-83 9.561387293553713E-4 84-85 1.274851639140495E-4 86-87 0.0014660793850115692 88-89 19.20130544807848 90-91 26.253848458385654 92-93 40.27109720106323 94-95 44.64249972909403 96-97 48.41593309578598 98-99 93.030258603655 100-101 51.706325176407596 102-103 53.07787431237689 104-105 74.8273532167694 106-107 81.60178734199808 108-109 12.608983879501023 110-111 39.08994715739956 112-113 51.99622643914814 114-115 86.8227510023521 116-117 66.41416105200757 118-119 71.93465110497766 120-121 80.37690988711189 122-123 75.84564096353287 124-125 50.08567003015024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 784405.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.42873464345303 #Duplication Level Percentage of deduplicated Percentage of total 1 86.7680929518732 66.31575551737184 2 9.334006928236995 14.267726773567546 3 2.0802042305528046 4.769621314233261 4 0.7298013926074309 2.2311118791206326 5 0.32089218071623615 1.2262691664560095 6 0.1887143986145601 0.865392161706662 7 0.11752967072735511 0.6287850811727402 8 0.07973785296670965 0.4875410564345061 9 0.058595843321915864 0.4030565544414051 >10 0.2903658094014643 3.9327037783242043 >50 0.019369468753605575 1.0501605312470401 >100 0.010351774748713549 1.542787424841583 >500 0.001335712845148202 0.7425541713739962 >1k 0.0010017846338611516 1.5365345897085902 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4455 0.5679464052370906 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2225 0.28365448970876017 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1473 0.18778564644539492 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1454 0.18536342833102798 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1419 0.18090144759403626 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1015 0.12939744137276024 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 899 0.11460916235873049 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 864 0.11014718162173877 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 1.274851639140495E-4 0.0 0.0 0.0 5 0.0 1.274851639140495E-4 0.0 0.0 0.0 6 0.0 1.274851639140495E-4 0.0 0.0 0.0 7 0.0 1.274851639140495E-4 0.0 0.0 0.0 8 0.0 1.274851639140495E-4 0.0 0.0 0.0 9 0.0 1.274851639140495E-4 0.0 0.0 0.0 10-11 0.0 1.274851639140495E-4 0.0 0.0 0.0 12-13 0.0 1.274851639140495E-4 0.0 0.0 0.0 14-15 0.0 1.274851639140495E-4 0.0 0.0 0.0 16-17 1.274851639140495E-4 1.274851639140495E-4 0.0 1.274851639140495E-4 0.0 18-19 1.274851639140495E-4 1.274851639140495E-4 0.0 2.54970327828099E-4 0.0 20-21 1.274851639140495E-4 1.274851639140495E-4 0.0 4.4619807369917326E-4 0.0 22-23 1.274851639140495E-4 1.274851639140495E-4 0.0 5.09940655656198E-4 0.0 24-25 1.274851639140495E-4 1.274851639140495E-4 0.0 8.286535654413217E-4 0.0 26-27 1.274851639140495E-4 1.9122774587107424E-4 0.0 0.0014023368030545444 0.0 28-29 2.54970327828099E-4 2.54970327828099E-4 0.0 0.0024222181143669407 0.0 30-31 2.54970327828099E-4 2.54970327828099E-4 0.0 0.0045894659009057814 0.0 32-33 2.54970327828099E-4 2.54970327828099E-4 0.0 0.010645011186823133 0.0 34-35 2.54970327828099E-4 2.54970327828099E-4 0.0 0.022182418521044613 0.0 36-37 2.54970327828099E-4 2.54970327828099E-4 0.0 0.037225667862902455 0.0 38-39 3.824554917421485E-4 2.54970327828099E-4 0.0 0.054117452081514014 0.0 40-41 3.824554917421485E-4 2.54970327828099E-4 0.0 0.07534373187320326 0.0 42-43 3.824554917421485E-4 2.54970327828099E-4 0.0 0.09389282322269746 0.0 44-45 3.824554917421485E-4 2.54970327828099E-4 0.0 0.1171588656370115 0.0 46-47 5.09940655656198E-4 2.54970327828099E-4 0.0 0.146862908828985 0.0 48-49 5.09940655656198E-4 2.54970327828099E-4 0.0 0.17981782370076682 0.0 50-51 5.09940655656198E-4 2.54970327828099E-4 0.0 0.2119440850071073 0.0 52-53 5.09940655656198E-4 2.54970327828099E-4 0.0 0.24502648504280317 0.0 54-55 5.09940655656198E-4 2.54970327828099E-4 0.0 0.2799574199552527 0.0 56-57 5.09940655656198E-4 2.54970327828099E-4 0.0 0.3268082176936659 0.0 58-59 5.09940655656198E-4 2.54970327828099E-4 0.0 0.3767186593660163 0.0 60-61 5.09940655656198E-4 2.54970327828099E-4 0.0 0.4334495573077683 0.0 62-63 5.09940655656198E-4 2.54970327828099E-4 0.0 0.4906903959051765 0.0 64-65 5.09940655656198E-4 2.54970327828099E-4 0.0 0.5499072545432525 0.0 66-67 5.09940655656198E-4 2.54970327828099E-4 0.0 0.615562113958988 0.0 68-69 6.374258195702476E-4 2.54970327828099E-4 0.0 0.6931368362006871 0.0 70-71 6.374258195702476E-4 2.54970327828099E-4 0.0 0.7902167885212359 0.0 72-73 7.011684015272722E-4 2.54970327828099E-4 0.0 0.8787552348595432 0.0 74-75 7.64910983484297E-4 2.54970327828099E-4 0.0 0.9697158993122175 0.0 76-77 7.64910983484297E-4 2.54970327828099E-4 0.0 1.071449060115629 0.0 78-79 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0975835187180092 0.0 80-81 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 82-83 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 84-85 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 86-87 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 88-89 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 90-91 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 92-93 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 94-95 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 96-97 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 98-99 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 100-101 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 102-103 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 104-105 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 106-107 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 108-109 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 110-111 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 112-113 7.64910983484297E-4 2.54970327828099E-4 0.0 1.0981572019556225 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCG 65 0.0 3471.7834 108-109 TTGCGCG 10 8.7386876E-4 3054.4683 110-111 CATCGCG 20 1.3694771E-6 2507.3994 108-109 CCGCGTA 10 0.0012977911 2507.3994 108-109 CCGCTCC 170 0.0 2064.917 108-109 CTCGACG 20 0.0034947193 1527.2341 110-111 CGTCGCT 20 0.0034947193 1527.2341 110-111 CGTCGCG 20 0.0034947193 1527.2341 110-111 CCGCGCC 220 0.0 1481.6449 108-109 CCGCGCG 95 0.0 1319.6838 108-109 CGTGACG 25 0.0054599084 1221.7874 110-111 CGCTAGC 25 0.0054599084 1221.7874 110-111 CGTCGGC 50 1.1800523E-5 1221.7874 110-111 CGGCGCG 30 0.002271575 1018.1561 110-111 CGCCGCC 120 1.2187229E-10 1018.1561 110-111 GGACGCC 50 2.1379095E-5 1002.9597 108-109 CGTCGCC 80 6.899427E-8 940.2748 108-109 CCGCACC 60 3.693215E-5 835.79974 108-109 TTGTTCG 100 1.6833837E-7 752.2198 108-109 CTCCGCC 125 1.8569517E-7 733.0723 110-111 >>END_MODULE