FastQCFastQC Report
Fri 27 May 2016
SRR939981_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939981_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784405
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT47860.6101439944926409No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT21820.278172627660456No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18460.23533761258533537No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14550.185490913494942No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC14210.18115641792186435No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT14180.1807739624301222No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.14954009727118006No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10640.13564421440454866No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC10340.13181965948712718No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG9950.12684773809447925No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA8360.10657759703214538No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8320.1060676563764892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8040.10249807178689581No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG350.007797812650.9959038
TAGGACC6650.027.7700164
ACCCGCC1107.7526685E-427.0432827
CTCGTAC900.00953088326.442328
CACCGTC1608.74217E-626.0291587
TACATGG32300.024.8981652
GTATAAC2451.5954356E-824.3194181
GTACATG34350.024.2840181
ACATGGG31450.024.2451783
GAGTACT27150.023.22833612-13
GTAGGAC17950.022.8992233
GTACTTT27400.022.36570514-15
AGTACTT26500.022.11426212-13
CATGGGG14850.022.0633934
GACCCGG1754.7841453E-420.398366
CCTAGAC4403.6379788E-1220.3083523
TGTAGGA22700.019.944532
GTGTAGG4503.6379788E-1219.8608571
CTAGTAC2701.0918575E-619.8570563
GTACTAG3352.0252628E-819.5644261
Produced by FastQC (version 0.11.5)