##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939981_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 784405 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.83463644418381 34.0 31.0 34.0 31.0 34.0 2 32.903684958662936 34.0 31.0 34.0 31.0 34.0 3 32.953181073552564 34.0 33.0 34.0 31.0 34.0 4 36.35213697006011 37.0 37.0 37.0 35.0 37.0 5 36.256996067082696 37.0 37.0 37.0 35.0 37.0 6 36.39537101369828 37.0 37.0 37.0 35.0 37.0 7 36.39342304039367 37.0 37.0 37.0 35.0 37.0 8 36.414612349487825 37.0 37.0 37.0 35.0 37.0 9 38.252149081150684 39.0 39.0 39.0 37.0 39.0 10-11 38.23917937799989 39.0 39.0 39.0 37.0 39.0 12-13 38.22437580076618 39.0 39.0 39.0 37.0 39.0 14-15 39.7524461215826 41.0 40.0 41.0 37.0 41.0 16-17 39.78924662642385 41.0 40.0 41.0 37.5 41.0 18-19 39.82407939776009 41.0 40.0 41.0 37.5 41.0 20-21 39.834895239066554 41.0 40.0 41.0 38.0 41.0 22-23 39.787547249188876 41.0 40.0 41.0 37.5 41.0 24-25 39.750592487299286 41.0 40.0 41.0 37.0 41.0 26-27 39.6542047794188 41.0 40.0 41.0 37.0 41.0 28-29 39.578659620986606 41.0 40.0 41.0 37.0 41.0 30-31 39.477070518418415 41.0 39.5 41.0 36.5 41.0 32-33 39.339181290277345 41.0 39.0 41.0 36.0 41.0 34-35 39.23627845309502 41.0 39.0 41.0 35.0 41.0 36-37 39.228046098635275 41.0 39.0 41.0 35.0 41.0 38-39 39.29212970340576 41.0 39.5 41.0 35.0 41.0 40-41 39.24087110612503 41.0 40.0 41.0 35.0 41.0 42-43 39.13810722777137 41.0 39.5 41.0 35.0 41.0 44-45 39.00081399277159 41.0 39.0 41.0 35.0 41.0 46-47 38.82441914572192 41.0 39.0 41.0 35.0 41.0 48-49 38.62483028537554 41.0 39.0 41.0 35.0 41.0 50-51 38.47776340028429 41.0 39.0 41.0 35.0 41.0 52-53 38.38156245816893 41.0 39.0 41.0 35.0 41.0 54-55 38.27381454733205 41.0 39.0 41.0 35.0 41.0 56-57 38.127539345108715 41.0 38.0 41.0 34.0 41.0 58-59 37.96966363039501 40.0 38.0 41.0 34.0 41.0 60-61 37.76574537388211 40.0 37.5 41.0 34.0 41.0 62-63 37.56759135905559 40.0 37.0 41.0 34.0 41.0 64-65 37.32319018874179 39.5 36.5 41.0 33.5 41.0 66-67 37.03739841026 39.0 36.0 41.0 33.0 41.0 68-69 36.69491334195983 39.0 35.5 41.0 33.0 41.0 70-71 36.319871112499285 38.5 35.0 40.5 32.0 41.0 72-73 35.94731803086416 37.0 35.0 40.0 32.0 41.0 74-75 35.58536087862775 37.0 35.0 39.0 32.0 41.0 76-77 35.187925880125704 36.0 35.0 39.0 31.5 41.0 78-79 34.809175107246894 36.0 35.0 38.0 31.0 39.5 80-81 34.50388511037028 35.5 35.0 37.0 31.0 39.0 82-83 34.24619871112499 35.0 35.0 37.0 31.0 39.0 84-85 33.969345554911044 35.0 35.0 36.5 31.0 38.0 86-87 33.76139048068281 35.0 35.0 36.0 31.0 37.0 88-89 33.54644921947208 35.0 35.0 36.0 31.0 37.0 90-91 33.36985358328924 35.0 35.0 35.5 31.0 36.5 92-93 33.229419751276446 35.0 34.0 35.0 31.0 36.0 94-95 33.11992975567468 35.0 34.0 35.0 31.0 36.0 96-97 33.02169861232399 35.0 34.0 35.0 31.0 36.0 98-99 32.942710079614486 35.0 34.0 35.0 30.0 36.0 100-101 32.89936767358699 35.0 34.0 35.0 30.0 36.0 102-103 32.0439677207565 34.5 32.5 35.0 28.0 35.0 104-105 32.7150477113226 35.0 34.0 35.0 30.0 35.0 106-107 32.77187677284056 35.0 34.0 35.0 30.0 35.0 108-109 32.78529904832325 35.0 34.0 35.0 30.0 35.0 110-111 32.76137008305658 35.0 34.0 35.0 30.0 35.0 112-113 32.68576564402318 35.0 34.0 35.0 30.0 35.0 114-115 32.62872623198476 35.0 34.0 35.0 30.0 35.0 116-117 32.57438313116312 35.0 34.0 35.0 30.0 35.0 118-119 32.4982604649384 35.0 34.0 35.0 29.5 35.0 120-121 32.41066795851633 35.0 34.0 35.0 29.0 35.0 122-123 32.24867702271148 35.0 34.0 35.0 29.0 35.0 124-125 30.23508200483169 33.5 30.5 34.5 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0182033937431072 1101 2 0.10674113479082337 1101 3 0.15189994907500903 1101 4 0.08401665212097953 1101 5 7.880352400633228E-4 1101 6 0.05453413164067911 1101 7 0.03207034653597418 1101 8 0.09814942687066974 1101 9 3.1924480160228086E-4 1101 10-11 0.138525482086024 1101 12-13 0.11563319044246612 1101 14-15 0.21948602477105084 1101 16-17 0.24871753999492796 1101 18-19 0.2260411784722436 1101 20-21 0.18019244184375793 1101 22-23 0.16139995741272628 1101 24-25 0.20326176304973131 1101 26-27 0.19952858819547714 1101 28-29 0.18369271182775293 1101 30-31 0.2223734442603984 1101 32-33 0.1446579013054361 1101 34-35 0.23788142057132688 1101 36-37 0.20426274828144386 1101 38-39 -0.07319165911690106 1101 40-41 -0.11332826961941578 1101 42-43 -0.11623742218658606 1101 44-45 -0.04419770682441282 1101 46-47 -0.08545916243242857 1101 48-49 -0.07841798761299401 1101 50-51 0.0030104634539824815 1101 52-53 -0.010808596419337846 1101 54-55 -0.014242231182990395 1101 56-57 0.0414378054479414 1101 58-59 0.09692184976092477 1101 60-61 0.07100047417679889 1101 62-63 0.0949985330011458 1101 64-65 -0.04872185035800669 1101 66-67 -0.0010068163767655847 1101 68-69 -0.026922227141753297 1101 70-71 0.010101287976461037 1101 72-73 -0.04254488309662463 1101 74-75 -0.057199230222828135 1101 76-77 -0.004010514580279789 1101 78-79 -0.011042693037929041 1101 80-81 0.00810105905587477 1101 82-83 0.01427852445394251 1101 84-85 -0.030402066028301533 1101 86-87 -0.10180192904566354 1101 88-89 -0.11361681637671239 1101 90-91 -0.07115872961531977 1101 92-93 -0.08989258842159131 1101 94-95 -0.08938887563227382 1101 96-97 0.020506031543916947 1101 98-99 0.011124751854644899 1101 100-101 -0.05766587097951259 1101 102-103 0.0661503343764096 1101 104-105 -0.1353175784688574 1101 106-107 -3.6244859420264675E-4 1101 108-109 -0.027014813554686157 1101 110-111 0.032578795092852886 1101 112-113 0.043754233743648285 1101 114-115 0.08184887803200525 1101 116-117 0.1594938201657925 1101 118-119 0.044531440242089104 1101 120-121 0.17292458624982032 1101 122-123 0.10408933661913977 1101 124-125 0.22714971320071342 1102 1 0.08064267946624426 1102 2 0.08738283974279426 1102 3 0.1531549519314197 1102 4 0.11247330450420634 1102 5 0.0026622720486884077 1102 6 0.017824884716802103 1102 7 0.09634831892095264 1102 8 0.05849943873449348 1102 9 0.032558267453659084 1102 10-11 0.10953516412716624 1102 12-13 0.05854172156827531 1102 14-15 0.022056217709142345 1102 16-17 0.19676554757455733 1102 18-19 0.15411661753722683 1102 20-21 0.13271150044280233 1102 22-23 0.14652883496231084 1102 24-25 0.15361763655403848 1102 26-27 0.13827857889456396 1102 28-29 0.07169693485449358 1102 30-31 0.06406946735641839 1102 32-33 0.02750280593596699 1102 34-35 0.1424309299379587 1102 36-37 0.18592755338810463 1102 38-39 0.15286097659578246 1102 40-41 0.07517390348664321 1102 42-43 -0.005638021909291524 1102 44-45 0.04685531332590642 1102 46-47 0.10791296740993772 1102 48-49 0.06642819186233595 1102 50-51 0.022804041113275275 1102 52-53 0.05730879181885484 1102 54-55 0.06669677220584447 1102 56-57 0.04449139770759558 1102 58-59 0.00839661039683648 1102 60-61 -0.029859426001692668 1102 62-63 0.03711958703819107 1102 64-65 0.11856143222680515 1102 66-67 0.09485084744946448 1102 68-69 0.08201417033865255 1102 70-71 0.1801847646816057 1102 72-73 0.20355183479808403 1102 74-75 0.06330625979461502 1102 76-77 0.12196308406187484 1102 78-79 0.1628682116802409 1102 80-81 0.2678829299691614 1102 82-83 0.05721690116810407 1102 84-85 0.05048443888794907 1102 86-87 0.11412966255094403 1102 88-89 0.08788885635194532 1102 90-91 0.16190596586460515 1102 92-93 0.19980638450986277 1102 94-95 0.15637507635353387 1102 96-97 0.10037067642689124 1102 98-99 0.18157117075607943 1102 100-101 0.25020625431574217 1102 102-103 0.21084811265758674 1102 104-105 0.2085234412746999 1102 106-107 0.22571540292027237 1102 108-109 0.25500780791313815 1102 110-111 0.11094702682029123 1102 112-113 0.08994172852480631 1102 114-115 0.0899782871802941 1102 116-117 0.07284571122691119 1102 118-119 -0.023471030079740274 1102 120-121 -0.10030805473751059 1102 122-123 0.004910627684729718 1102 124-125 0.14722347562156202 1103 1 0.07933249064539183 1103 2 0.10275167811482788 1103 3 0.028033537056892044 1103 4 2.9687868313743593E-4 1103 5 -0.16079672348939056 1103 6 -0.0641816128492394 1103 7 -0.04340919909616758 1103 8 -0.013596531787875676 1103 9 0.10711367266998906 1103 10-11 0.050908258186588284 1103 12-13 0.03241882100432747 1103 14-15 0.11149803683353099 1103 16-17 0.11473842666596568 1103 18-19 0.09231371684388279 1103 20-21 0.024978798372458755 1103 22-23 0.05627340475533771 1103 24-25 0.15571555618446808 1103 26-27 0.13501038566921153 1103 28-29 0.14707970004844384 1103 30-31 0.13723744731214538 1103 32-33 0.15989900380035493 1103 34-35 0.1567390814960774 1103 36-37 0.12956840636955036 1103 38-39 0.12356005938273285 1103 40-41 0.17766301961870568 1103 42-43 0.15904139025265351 1103 44-45 0.12181469342866791 1103 46-47 0.04607569447900062 1103 48-49 -0.0471409161497931 1103 50-51 0.03956717919342623 1103 52-53 0.029527936265559163 1103 54-55 0.14505455565751646 1103 56-57 0.09799684024813615 1103 58-59 -0.011088404520720019 1103 60-61 0.09447466244252212 1103 62-63 0.08683518710063254 1103 64-65 0.08760741257559346 1103 66-67 0.18016476302963724 1103 68-69 0.19338224200759413 1103 70-71 0.2260907076819194 1103 72-73 0.15270154651609857 1103 74-75 0.041731817211207556 1103 76-77 0.08456164139840183 1103 78-79 0.18592874806507353 1103 80-81 0.1674537218165426 1103 82-83 0.14574280125569317 1103 84-85 0.11643060953761619 1103 86-87 0.07994101315343016 1103 88-89 0.10449630841480229 1103 90-91 0.15226596854968477 1103 92-93 0.1706967386894931 1103 94-95 0.2412037368148603 1103 96-97 0.11215388384999869 1103 98-99 0.2283610868312067 1103 100-101 0.27208398134841616 1103 102-103 0.2515310930621766 1103 104-105 0.19142547716255365 1103 106-107 0.3025430152305546 1103 108-109 0.3213324775555009 1103 110-111 0.2781950560779407 1103 112-113 0.3224234121659606 1103 114-115 0.4584130887069122 1103 116-117 0.45517700621916646 1103 118-119 0.3524678374803827 1103 120-121 0.35331894421028665 1103 122-123 0.4073250145284959 1103 124-125 0.4963649380025643 1104 1 0.021292406006715225 1104 2 0.03975546578215017 1104 3 0.0971794994601467 1104 4 0.06156163088854072 1104 5 -0.08958747708342685 1104 6 0.001852124674933009 1104 7 -0.03264458095704015 1104 8 -0.053445276715173407 1104 9 -0.06484500818071126 1104 10-11 0.021710902398268672 1104 12-13 0.03710170510422728 1104 14-15 0.02581749515857723 1104 16-17 0.08571366647161938 1104 18-19 0.14657085204009235 1104 20-21 0.15750351414014574 1104 22-23 0.09914788018660659 1104 24-25 0.0582120197439977 1104 26-27 0.06057568538401625 1104 28-29 0.1271219093034972 1104 30-31 0.02231669581755824 1104 32-33 0.019694373835086765 1104 34-35 -0.02751320215200792 1104 36-37 -0.07420290564790122 1104 38-39 -0.057064571541843634 1104 40-41 -0.035188236004401574 1104 42-43 -0.09600732573834847 1104 44-45 0.019883824451135013 1104 46-47 -0.10435414324968662 1104 48-49 0.011669361583649618 1104 50-51 -0.0620794378149796 1104 52-53 -0.08350487891404867 1104 54-55 -0.1352265353353559 1104 56-57 -0.1287536682994741 1104 58-59 -0.09872765063574462 1104 60-61 -0.12721395937969504 1104 62-63 -0.20368998315568376 1104 64-65 -0.2243337649588284 1104 66-67 -0.27386072450376986 1104 68-69 -0.20158645882330006 1104 70-71 -0.18332569904293905 1104 72-73 -0.10526517516622391 1104 74-75 -0.25559652880403405 1104 76-77 -0.26841364715862426 1104 78-79 -0.18959129936529706 1104 80-81 -0.08362765445049547 1104 82-83 -0.038649446017572586 1104 84-85 -0.026543413056288045 1104 86-87 -0.0913862587589378 1104 88-89 0.025000900872960585 1104 90-91 0.030907283452897616 1104 92-93 0.0037696715329005315 1104 94-95 -0.0704299925316505 1104 96-97 -0.06826669898055115 1104 98-99 0.03270845138690959 1104 100-101 0.050235344932232806 1104 102-103 0.0229351090746448 1104 104-105 0.20074131451853106 1104 106-107 0.12907343490912382 1104 108-109 0.10665107958622855 1104 110-111 0.16924005752868254 1104 112-113 0.19042002158646198 1104 114-115 0.18686203225674802 1104 116-117 0.21576922534244147 1104 118-119 0.29328052290270534 1104 120-121 0.15312653354128258 1104 122-123 0.21216618333484405 1104 124-125 0.36316753189024453 1105 1 0.11189136129429755 1105 2 0.14084711279146944 1105 3 0.0907005509165657 1105 4 0.08125821165022273 1105 5 -0.050305196639357064 1105 6 0.07578929915162291 1105 7 0.05228349063872173 1105 8 0.16630051701867643 1105 9 0.16906698262071984 1105 10-11 0.14115488694782385 1105 12-13 0.2034183875423352 1105 14-15 0.17440877383006637 1105 16-17 0.15383376131291016 1105 18-19 0.08861460297992352 1105 20-21 0.11071853381774588 1105 22-23 0.14760329156301566 1105 24-25 0.14308085350288025 1105 26-27 0.24222395541134034 1105 28-29 0.21997038347351605 1105 30-31 0.11800554831990695 1105 32-33 0.2287241947697467 1105 34-35 0.41082362722632126 1105 36-37 0.37713435054663336 1105 38-39 0.2885487147455166 1105 40-41 0.3277543959478777 1105 42-43 0.43868630072607573 1105 44-45 0.4208685395703 1105 46-47 0.37133176254999967 1105 48-49 0.46278941237628857 1105 50-51 0.46801300358892206 1105 52-53 0.461204878469033 1105 54-55 0.3871141034915553 1105 56-57 0.5820548066603877 1105 58-59 0.40287177095994053 1105 60-61 0.5227553584586389 1105 62-63 0.4712477031966813 1105 64-65 0.4213191651116617 1105 66-67 0.4512126772517959 1105 68-69 0.45440575216061063 1105 70-71 0.47594661729318233 1105 72-73 0.48158964504693813 1105 74-75 0.6208918218475077 1105 76-77 0.44395323858677926 1105 78-79 0.40811771526644947 1105 80-81 0.3177098295818652 1105 82-83 0.425794181586042 1105 84-85 0.3203077921299098 1105 86-87 0.29144585665459743 1105 88-89 0.33895042416026655 1105 90-91 0.25602891088335866 1105 92-93 0.2298349273264293 1105 94-95 0.3740542813415715 1105 96-97 0.4240020038475194 1105 98-99 0.37023653239997145 1105 100-101 0.5070775429293874 1105 102-103 0.3569203117667783 1105 104-105 0.48610858151257474 1105 106-107 0.431686507028914 1105 108-109 0.3392032582475437 1105 110-111 0.3090903376932701 1105 112-113 0.36942347408910337 1105 114-115 0.4843616639189108 1105 116-117 0.41326019916691337 1105 118-119 0.4146311303943193 1105 120-121 0.25528825891163365 1105 122-123 0.3225490572205345 1105 124-125 0.18936865497887467 1106 1 0.04606327401262433 1106 2 0.059653616317767444 1106 3 3.097003462073644E-4 1106 4 0.03228503751286382 1106 5 0.0368966067401999 1106 6 0.05717123932296886 1106 7 -0.013420348051106146 1106 8 -0.02061669791955012 1106 9 -0.03612512936867773 1106 10-11 0.08869831478897794 1106 12-13 0.08079464186145202 1106 14-15 -0.08693038932608488 1106 16-17 -0.005163759631841458 1106 18-19 -0.09538498877470403 1106 20-21 0.040779149380767876 1106 22-23 0.04744191630859973 1106 24-25 0.03106778965094037 1106 26-27 -0.008159883988476224 1106 28-29 0.005383450875420692 1106 30-31 0.04699599892813211 1106 32-33 0.028672128693152388 1106 34-35 0.21254358273348828 1106 36-37 0.1669870863822922 1106 38-39 0.1582378270189082 1106 40-41 0.028164416300981543 1106 42-43 0.057922053554207764 1106 44-45 0.08728547642834883 1106 46-47 0.10408931213110861 1106 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-0.5204460067851571 2216 36-37 -0.37236048674008515 2216 38-39 -0.37853974830623827 2216 40-41 -0.21639210868609382 2216 42-43 -0.23849114295200025 2216 44-45 -0.30219803392466815 2216 46-47 -0.3269681849478303 2216 48-49 -0.2444486260036598 2216 50-51 -0.3187198969014702 2216 52-53 -0.26668277185410716 2216 54-55 -0.4762713431057932 2216 56-57 -0.4719187883480558 2216 58-59 -0.542368342683055 2216 60-61 -0.4970908958281086 2216 62-63 -0.601179845671922 2216 64-65 -0.3978468571725955 2216 66-67 -0.4337877137217987 2216 68-69 -0.5292664456447227 2216 70-71 -0.4730530488277722 2216 72-73 -0.5377474126719335 2216 74-75 -0.5172112238988902 2216 76-77 -0.4567990442646206 2216 78-79 -0.5252715541838668 2216 80-81 -0.5051192429666997 2216 82-83 -0.4912821799019369 2216 84-85 -0.4384596356733965 2216 86-87 -0.41772625985305467 2216 88-89 -0.4563361098401586 2216 90-91 -0.4153227158771031 2216 92-93 -0.4462955759372633 2216 94-95 -0.4078482334254332 2216 96-97 -0.4125474493479686 2216 98-99 -0.4396466595829551 2216 100-101 -0.3766158791570149 2216 102-103 -0.3688930064075393 2216 104-105 -0.5197966713108144 2216 106-107 -0.6416697592849232 2216 108-109 -0.5604608147540517 2216 110-111 -0.5743284719845008 2216 112-113 -0.5567200616278853 2216 114-115 -0.5479554069813517 2216 116-117 -0.6930929976938813 2216 118-119 -0.5951131108128109 2216 120-121 -0.6186035171138613 2216 122-123 -0.6860213323008786 2216 124-125 -0.6876406700517528 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59.0 3 8.0 4 7.0 5 25.0 6 46.0 7 127.0 8 220.0 9 437.0 10 1018.0 11 1512.0 12 2018.0 13 3122.0 14 4611.0 15 5046.0 16 2021.0 17 1431.0 18 1250.0 19 1167.0 20 1192.0 21 1201.0 22 1316.0 23 1445.0 24 1746.0 25 2107.0 26 2722.0 27 3656.0 28 4490.0 29 5939.0 30 7597.0 31 9928.0 32 12662.0 33 16796.0 34 24529.0 35 40390.0 36 89765.0 37 289065.0 38 240936.0 39 2789.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6934473719106 19.695370115364607 14.191580729724492 23.419601783000296 2 14.014189098743635 21.044358462783894 42.28019964176669 22.66125279670578 3 20.182686239888834 25.607307449595552 28.491149342495266 25.718856968020347 4 11.835595132616442 17.639612190131373 39.367673586986314 31.157119090265873 5 12.756270923819745 38.12292835837278 34.66152391138703 14.459276806420446 6 29.44639567570324 37.69838285069575 18.966350290984888 13.888871182616121 7 27.426903194140785 31.937073323092026 22.383717594864898 18.252305887902295 8 23.876058923642763 36.78546159190724 20.695176598823313 18.643302885626685 9 24.836787118899036 18.322805183546762 22.14634021965694 34.69406747789726 10-11 23.98875580854278 26.84531587636489 28.870800160631305 20.295128154461025 12-13 26.291137868830518 24.24557467124763 28.753067611756684 20.71021984816517 14-15 21.60809785761182 27.126293177631457 27.408927786028904 23.856681178727825 16-17 20.62548165580597 29.353068924348246 29.016634933423934 21.004814486421846 18-19 20.74279230754521 28.275826900644436 30.595037002568827 20.386343789241526 20-21 21.657375800400956 27.28633398034735 29.798087005855002 21.258203213396694 22-23 20.703680756344944 28.191212691419214 29.894926593949034 21.2101799582868 24-25 21.48023023820603 27.410330122831954 30.517717250654957 20.59172238830706 26-27 20.60122003301866 27.807956349079877 30.80379395847808 20.787029659423382 28-29 20.149348869525312 28.601487751862877 29.786398607862008 21.462764770749803 30-31 20.991133406849777 27.41383596483959 30.186510794806253 21.408519833504375 32-33 20.497255881846748 27.95781515926084 30.67713744812947 20.867791510762935 34-35 21.148832554611456 27.891522874025537 30.45703431263187 20.502610258731142 36-37 20.668978397638973 27.53858019772949 30.680707032719067 21.11173437191247 38-39 21.04716313639 28.17192649205449 30.132775798216482 20.64813457333903 40-41 21.703329275055616 27.462152841963018 30.383347887889546 20.451169995091824 42-43 20.584519476545918 28.441876326642486 29.87232360834008 21.101280588471518 44-45 20.75503088328096 28.165169778367044 29.50784352470981 21.571955813642187 46-47 21.746674230786393 28.006195778966227 29.113340684977786 21.133789305269598 48-49 21.575397913067867 28.0191355231035 29.072864145435073 21.33260241839356 50-51 20.514912577048847 28.906623491691153 29.459207934676602 21.119255996583398 52-53 20.920060427967694 28.775441258023598 29.083764126949724 21.22073418705898 54-55 20.745214525659577 27.98375839011735 29.432117337344867 21.83890974687821 56-57 21.42796132100127 28.09734767116477 28.590842740676052 21.88384826715791 58-59 21.98513522988762 28.199272059714055 28.27990642588969 21.53568628450864 60-61 21.15590798120869 27.75817339257144 29.999936257418042 21.08598236880183 62-63 21.160624932273507 27.7414728360987 29.8289786526093 21.26892357901849 64-65 21.111351916420727 28.678934988940664 28.786150011792376 21.423563082846233 66-67 21.31717671355996 28.697866535781895 28.32350635194829 21.66145039870985 68-69 21.371485393387342 27.943919276394208 29.328471899082743 21.3561234311357 70-71 22.179868817766334 27.808657517481407 28.712336101886144 21.29913756286612 72-73 21.60357213429287 27.987200489543028 29.175680930131755 21.233546446032342 74-75 20.91629961563223 28.973107004672332 29.184668634187695 20.92592474550774 76-77 21.309081405651416 27.68939514663981 29.367673586986314 21.63384986072246 78-79 21.257258686520355 27.78067452400227 29.285700626589577 21.676366162887795 80-81 21.608480313103563 28.147640568328864 28.979226292540204 21.264652826027373 82-83 21.28441302643405 28.028059484577483 29.698051389269576 20.989476099718896 84-85 21.17643309259885 28.12386458525889 29.518106080404895 21.18159624173737 86-87 20.672229269318784 28.93008076185134 28.667142611278614 21.730547357551263 88-89 20.939565658046543 28.299921596624195 28.79896227076574 21.961550474563523 90-91 21.319652318471793 27.995043319955737 29.202979107491622 21.48232525408085 92-93 21.225196167795975 28.73184133196499 29.214245192215753 20.82871730802328 94-95 21.00101988781234 28.68663946965834 28.900433452320247 21.411907190209075 96-97 21.106379995028078 28.349194612476975 29.24108081921966 21.303344573275286 98-99 21.40562819874565 28.220227323287727 29.00179056450909 21.372353913457538 100-101 20.901105107256658 27.975844064948717 29.39176074558566 21.731290082208965 102-103 21.11332157488426 28.159658235811936 29.03650622354269 21.690513965761113 104-105 21.689939558050916 27.795264849456075 28.895038048723276 21.619757543769737 106-107 21.182983237203516 27.778776473948053 29.311371367083318 21.726868921765117 108-109 20.810351344753094 28.32277643882175 28.84374754988045 22.023124666544707 110-111 20.61609229400163 28.751611180027613 28.98600520422304 21.64629132174771 112-113 21.10954857414606 27.86015449991521 28.606198611056215 22.424098314882507 114-115 20.692079726095336 28.218912094629957 28.225860430248588 22.863147749026115 116-117 21.324441509400284 28.090773606530416 27.85045978344984 22.73432510061946 118-119 21.203909982438862 27.516612963370207 28.913982298628575 22.365494755562352 120-121 20.66230492977408 28.230411386071086 28.87740519150379 22.229878492651043 122-123 21.04257354867538 28.026869098560613 28.467909364599876 22.462647988164132 124-125 21.275404962302108 28.144465663939293 28.14523058564956 22.434898788109038 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 38.0 1 30.5 2 69.5 3 115.5 4 68.5 5 38.0 6 110.5 7 243.0 8 426.5 9 475.0 10 346.5 11 282.5 12 307.0 13 357.0 14 417.5 15 475.5 16 590.0 17 721.0 18 861.0 19 1009.5 20 1160.5 21 1436.0 22 1812.5 23 2239.0 24 2802.0 25 3666.5 26 4625.5 27 5772.0 28 7065.5 29 8786.0 30 10907.0 31 13158.0 32 16114.5 33 19634.0 34 23588.5 35 27473.5 36 30442.0 37 32003.5 38 31655.5 39 29938.5 40 28557.0 41 28156.5 42 27910.5 43 27590.0 44 27378.5 45 27420.0 46 27443.5 47 30234.0 48 37930.0 49 36857.5 50 28791.0 51 24987.5 52 22048.0 53 20085.0 54 18718.0 55 16576.5 56 14082.0 57 11841.5 58 10032.5 59 8476.5 60 6838.0 61 5429.5 62 4169.0 63 3155.5 64 2354.5 65 1698.0 66 1204.0 67 860.5 68 638.0 69 438.0 70 317.0 71 243.0 72 196.5 73 165.0 74 138.5 75 102.0 76 50.5 77 20.5 78 10.0 79 6.0 80 2.0 81 2.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 2.5 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013895882866631397 2 0.0 3 0.0 4 0.0 5 0.11575652883395694 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.9122774587107424E-4 18-19 0.0 20-21 0.004143267827206609 22-23 1.274851639140495E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0011473664752264454 92-93 0.0 94-95 6.374258195702476E-4 96-97 0.0 98-99 0.001976020040667767 100-101 1.9122774587107424E-4 102-103 5.736832376132227E-4 104-105 0.002039762622624792 106-107 0.006246773031788427 108-109 5.736832376132227E-4 110-111 0.005354376884390079 112-113 0.008668991146155366 114-115 0.005673089794175203 116-117 0.001784792294796693 118-119 3.187129097851238E-4 120-121 0.001019881311312396 122-123 0.0012111090571834703 124-125 0.0014023368030545446 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 784405.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.53304566792869 #Duplication Level Percentage of deduplicated Percentage of total 1 85.9262427881161 64.04344577800185 2 10.005172464005014 14.914319523503766 3 2.108478923896244 4.714540677738716 4 0.7722878112576653 2.3024385082100904 5 0.35359040211128745 1.3177084794150928 6 0.19706493459603958 0.8812709861876399 7 0.12860064307170477 0.6709498322091858 8 0.09470452958369457 0.5646893622736969 9 0.06413255410225084 0.4301995125333572 >10 0.30982442306222446 4.1525846357797 >50 0.023461605920667483 1.1985195159077497 >100 0.013527861514564546 1.9388190867644612 >500 0.001369910005944373 0.7800712368597938 >1k 0.00154114875668742 2.090442864614948 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4786 0.6101439944926409 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2182 0.278172627660456 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1846 0.23533761258533537 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1455 0.185490913494942 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1421 0.18115641792186435 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1418 0.1807739624301222 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1173 0.14954009727118006 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1064 0.13564421440454866 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1034 0.13181965948712718 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 995 0.12684773809447925 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 836 0.10657759703214538 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 832 0.1060676563764892 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 804 0.10249807178689581 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 2.54970327828099E-4 0.0 0.0 0.0 12-13 0.0 2.54970327828099E-4 0.0 0.0 0.0 14-15 0.0 2.54970327828099E-4 0.0 0.0 0.0 16-17 0.0 2.54970327828099E-4 0.0 0.0 0.0 18-19 0.0 2.54970327828099E-4 0.0 1.274851639140495E-4 0.0 20-21 0.0 2.54970327828099E-4 0.0 1.9122774587107424E-4 0.0 22-23 0.0 2.54970327828099E-4 0.0 2.54970327828099E-4 0.0 24-25 0.0 2.54970327828099E-4 0.0 4.4619807369917326E-4 0.0 26-27 0.0 2.54970327828099E-4 0.0 8.923961473983465E-4 0.0 28-29 0.0 2.54970327828099E-4 0.0 0.0019122774587107426 0.0 30-31 0.0 2.54970327828099E-4 0.0 0.004079525245249584 0.0 32-33 0.0 2.54970327828099E-4 0.0 0.010007585367252887 0.0 34-35 0.0 2.54970327828099E-4 0.0 0.021417507537560318 0.0 36-37 0.0 2.54970327828099E-4 0.0 0.036269529133547085 0.0 38-39 0.0 2.54970327828099E-4 0.0 0.05309757077020162 0.0 40-41 0.0 2.54970327828099E-4 0.0 0.07432385056189086 0.0 42-43 6.374258195702475E-5 3.824554917421485E-4 0.0 0.09268171416551399 0.0 44-45 1.274851639140495E-4 3.824554917421485E-4 0.0 0.11607524174374206 0.0 46-47 1.274851639140495E-4 3.824554917421485E-4 0.0 0.14577928493571563 0.0 48-49 2.54970327828099E-4 3.824554917421485E-4 0.0 0.17879794238945443 0.0 50-51 2.54970327828099E-4 3.824554917421485E-4 0.0 0.21060549078600976 0.0 52-53 2.54970327828099E-4 3.824554917421485E-4 0.0 0.24349666307583456 0.0 54-55 2.54970327828099E-4 3.824554917421485E-4 0.0 0.27823637024241304 0.0 56-57 2.54970327828099E-4 4.4619807369917326E-4 0.0 0.3248321976529981 0.0 58-59 2.54970327828099E-4 5.09940655656198E-4 0.0 0.3746788967433915 0.0 60-61 2.54970327828099E-4 5.09940655656198E-4 0.0 0.4323659334144989 0.0 62-63 2.54970327828099E-4 5.09940655656198E-4 0.0 0.4899254849216922 0.0 64-65 2.54970327828099E-4 5.09940655656198E-4 0.0 0.5495885416334674 0.0 66-67 2.54970327828099E-4 5.09940655656198E-4 0.0 0.6151796584672459 0.0 68-69 2.54970327828099E-4 5.09940655656198E-4 0.0 0.692881865872859 0.0 70-71 3.187129097851237E-4 5.09940655656198E-4 0.0 0.790408016267107 0.0 72-73 3.824554917421485E-4 5.09940655656198E-4 0.0 0.8792651755151994 0.0 74-75 3.824554917421485E-4 5.09940655656198E-4 0.0 0.9702895825498308 0.0 76-77 3.824554917421485E-4 5.09940655656198E-4 0.0 1.0728513969186837 0.0 78-79 3.824554917421485E-4 5.09940655656198E-4 0.0 1.1958745800957413 0.0 80-81 3.824554917421485E-4 5.09940655656198E-4 0.0 1.3345784384342272 0.0 82-83 3.824554917421485E-4 5.09940655656198E-4 0.0 1.480867664025599 0.0 84-85 3.824554917421485E-4 5.09940655656198E-4 0.0 1.625117126994346 0.0 86-87 3.824554917421485E-4 5.09940655656198E-4 0.0 1.7775893830355494 0.0 88-89 3.824554917421485E-4 5.09940655656198E-4 0.0 1.9458697994020946 0.0 90-91 3.824554917421485E-4 5.09940655656198E-4 0.0 2.1354402381422863 0.0 92-93 5.09940655656198E-4 5.09940655656198E-4 0.0 2.3345720641760312 0.0 94-95 5.09940655656198E-4 5.09940655656198E-4 0.0 2.5267559487764615 0.0 96-97 5.09940655656198E-4 5.09940655656198E-4 0.0 2.728118765178702 0.0 98-99 6.374258195702476E-4 5.09940655656198E-4 0.0 2.95013417813502 0.0 100-101 6.374258195702476E-4 5.09940655656198E-4 0.0 3.2005150400622124 0.0 102-103 6.374258195702476E-4 6.374258195702476E-4 0.0 3.479388836124196 0.0 104-105 0.001019881311312396 6.374258195702476E-4 0.0 3.742518214442794 0.0 106-107 0.001019881311312396 6.374258195702476E-4 0.0 4.012021850957095 0.0 108-109 0.001019881311312396 6.374258195702476E-4 0.0 4.3051739853774516 0.0 110-111 0.001019881311312396 6.374258195702476E-4 0.0 4.626245370694985 0.0 112-113 0.001019881311312396 6.374258195702476E-4 0.0 4.975618462401438 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTCG 35 0.0077978126 50.995903 8 TAGGACC 665 0.0 27.770016 4 ACCCGCC 110 7.7526685E-4 27.043282 7 CTCGTAC 90 0.009530883 26.44232 8 CACCGTC 160 8.74217E-6 26.029158 7 TACATGG 3230 0.0 24.898165 2 GTATAAC 245 1.5954356E-8 24.319418 1 GTACATG 3435 0.0 24.284018 1 ACATGGG 3145 0.0 24.245178 3 GAGTACT 2715 0.0 23.228336 12-13 GTAGGAC 1795 0.0 22.899223 3 GTACTTT 2740 0.0 22.365705 14-15 AGTACTT 2650 0.0 22.114262 12-13 CATGGGG 1485 0.0 22.063393 4 GACCCGG 175 4.7841453E-4 20.39836 6 CCTAGAC 440 3.6379788E-12 20.308352 3 TGTAGGA 2270 0.0 19.94453 2 GTGTAGG 450 3.6379788E-12 19.860857 1 CTAGTAC 270 1.0918575E-6 19.857056 3 GTACTAG 335 2.0252628E-8 19.564426 1 >>END_MODULE