FastQCFastQC Report
Fri 27 May 2016
SRR939980_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939980_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401499
Sequences flagged as poor quality0
Sequence length125
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT75400.5379953892225395No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40600.28968982496598283No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30180.21534086003628972No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25780.1839459036360354No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT24580.17538364279960242No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT18910.13492696034745655No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17340.12372466908645671No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17250.12308249952372424No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14330.1022476648217373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCC5350.01322.7244108-109
CCGCTCC5400.01263.6742108-109
CCGCTCG2500.01112.0333108-109
CGTCGTC602.1623002E-51000.40875110-111
CGTTTCG652.7488333E-5923.4543110-111
CTCCGCC4400.0682.0968110-111
CGTCGCT1403.1724085E-7643.1199110-111
CCTCGCC6800.0631.8371108-109
CGTCGCC2850.0620.75226108-109
CCGTTGC851.02966005E-4594.6702108-109
CCGCGCT1304.6994865E-7583.23425108-109
CGGCTCG1151.5204192E-4521.9524110-111
GTCGGGT1151.5204192E-4521.9524110-111
CCGTTCC2451.8189894E-11515.7854108-109
TTATGCG603.6733974E-5500.20438110-111
CCGCACC3100.0489.16425108-109
CGCCGCC4950.0485.04666110-111
CTTGTCG1251.9520559E-4480.19617110-111
CCTCTCG3006.184564E-11421.22473108-109
TTTCGCG757.79764E-5400.1635110-111
Produced by FastQC (version 0.11.5)