##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939980_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1401499 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.788395853297075 34.0 31.0 34.0 30.0 34.0 2 31.91231388677409 34.0 31.0 34.0 30.0 34.0 3 32.05565184134987 34.0 31.0 34.0 30.0 34.0 4 35.26971407043458 37.0 35.0 37.0 33.0 37.0 5 35.31854963863692 37.0 35.0 37.0 33.0 37.0 6 35.37516544785262 37.0 37.0 37.0 33.0 37.0 7 35.32699202782164 37.0 37.0 37.0 33.0 37.0 8 35.295634888073415 37.0 37.0 37.0 33.0 37.0 9 36.951982127707545 39.0 38.0 39.0 34.0 39.0 10-11 36.92641985474124 39.0 38.0 39.0 34.0 39.0 12-13 36.858859692372235 39.0 38.0 39.0 34.0 39.0 14-15 38.36307803287765 41.0 39.0 41.0 34.0 41.0 16-17 38.373117640469246 41.0 39.0 41.0 34.0 41.0 18-19 38.40798102602999 41.0 39.0 41.0 34.0 41.0 20-21 38.46016336793676 41.0 39.0 41.0 34.0 41.0 22-23 38.45482872267479 41.0 39.0 41.0 34.0 41.0 24-25 38.4768587062852 41.0 39.0 41.0 34.0 41.0 26-27 38.44151333679154 41.0 39.0 41.0 34.0 41.0 28-29 38.3856356658121 41.0 39.0 41.0 34.0 41.0 30-31 38.316193946624296 41.0 39.0 41.0 33.0 41.0 32-33 38.19507541567992 40.5 38.5 41.0 33.0 41.0 34-35 38.08800541420294 40.0 38.0 41.0 33.0 41.0 36-37 38.26681788570666 41.0 39.0 41.0 33.0 41.0 38-39 38.243430070231945 41.0 39.0 41.0 33.0 41.0 40-41 38.19925130164203 41.0 39.0 41.0 33.0 41.0 42-43 38.0956904000645 41.0 38.0 41.0 33.0 41.0 44-45 37.97353298147198 41.0 38.0 41.0 33.0 41.0 46-47 37.86219397944629 40.0 38.0 41.0 33.0 41.0 48-49 37.74075650428577 40.0 38.0 41.0 33.0 41.0 50-51 37.60096832034843 40.0 38.0 41.0 32.0 41.0 52-53 37.46070243360859 40.0 37.0 41.0 32.0 41.0 54-55 37.2712681207764 40.0 37.0 41.0 31.5 41.0 56-57 37.04199182446794 40.0 36.5 41.0 31.0 41.0 58-59 36.825582822392306 40.0 36.0 41.0 31.0 41.0 60-61 36.548833070876256 39.0 35.5 41.0 31.0 41.0 62-63 36.277577079969376 39.0 35.0 41.0 31.0 41.0 64-65 35.95902637104986 39.0 35.0 41.0 30.0 41.0 66-67 35.60757909923589 37.5 35.0 40.5 30.0 41.0 68-69 35.21727450394185 37.0 35.0 40.0 29.5 41.0 70-71 34.81032237625571 36.5 35.0 39.0 29.0 41.0 72-73 34.415810856804036 36.0 34.0 39.0 29.0 41.0 74-75 34.01856690586294 35.5 34.0 39.0 29.0 40.5 76-77 33.603954765576006 35.0 34.0 37.0 29.0 39.5 78-79 33.17644286581724 35.0 34.0 37.0 28.0 39.0 80-81 32.75462451275384 35.0 34.0 36.5 26.5 39.0 82-83 32.59804930292494 35.0 34.0 36.0 27.5 37.5 84-85 32.252932752716916 35.0 34.0 36.0 27.0 37.0 86-87 31.649026149858116 35.0 34.0 35.5 14.0 37.0 88-89 16.37487112013637 18.0 16.5 18.5 2.0 19.0 90-91 2.769872472260059 2.0 2.0 2.0 2.0 2.0 92-93 2.297706241674093 2.0 2.0 2.0 2.0 2.0 94-95 2.1042330390531854 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.028643901057261445 1101 2 0.14043354762545235 1101 3 0.16640192813503063 1101 4 0.18638742796790808 1101 5 0.15561896084621907 1101 6 0.1265304853127418 1101 7 0.15131236740536025 1101 8 0.21219085574971075 1101 9 0.2788482963336705 1101 10-11 0.17158393879291367 1101 12-13 0.19208385524678562 1101 14-15 0.363944542890863 1101 16-17 0.380196864544196 1101 18-19 0.26098488502184125 1101 20-21 0.29720082405805215 1101 22-23 0.33691584387708673 1101 24-25 0.3331039469660979 1101 26-27 0.4100153902360404 1101 28-29 0.4288098789781216 1101 30-31 0.380227317717754 1101 32-33 0.40493866958091473 1101 34-35 0.43052215724065945 1101 36-37 0.47093251874905917 1101 38-39 0.4657120571218698 1101 40-41 0.4544557245833758 1101 42-43 0.4335703917873346 1101 44-45 0.4755958086313541 1101 46-47 0.38857821406104165 1101 48-49 0.34057363120926 1101 50-51 0.42728164418263503 1101 52-53 0.44983675632673226 1101 54-55 0.38198828434467913 1101 56-57 0.49449426481812253 1101 58-59 0.40799129453987604 1101 60-61 0.32322637244128316 1101 62-63 0.3225672862306368 1101 64-65 0.35820183098256564 1101 66-67 0.4159573801129355 1101 68-69 0.3922682841072742 1101 70-71 0.3856216726860566 1101 72-73 0.3388213519941843 1101 74-75 0.4083094743131923 1101 76-77 0.3243036169277289 1101 78-79 0.3836031473346182 1101 80-81 0.39640225527872985 1101 82-83 0.43835043521973915 1101 84-85 0.5555863647204617 1101 86-87 0.695012900308182 1101 88-89 1.4624634418182474 1101 90-91 -0.04618673360750147 1101 92-93 -0.15625872749542946 1101 94-95 -0.0011081577815108545 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.008109567523241878 1102 2 0.1130698922847948 1102 3 0.08993977105050277 1102 4 0.13145010036843274 1102 5 0.13558786716851046 1102 6 0.12019163126865351 1102 7 0.15277156596861374 1102 8 0.10058019613406088 1102 9 0.06989314446884265 1102 10-11 0.20676951807137556 1102 12-13 0.04138947887513211 1102 14-15 0.038874160558492576 1102 16-17 0.09811227267537248 1102 18-19 -0.050720091744246076 1102 20-21 0.07721089572791584 1102 22-23 0.13902911689552866 1102 24-25 0.14166120946286753 1102 26-27 0.23479918962747348 1102 28-29 0.19836367302136182 1102 30-31 0.22176307722476452 1102 32-33 0.18363442134752006 1102 34-35 0.28666133014476713 1102 36-37 0.21512315113108116 1102 38-39 0.18612024163608254 1102 40-41 0.09838930844716742 1102 42-43 0.20161432417763336 1102 44-45 0.24156814125142034 1102 46-47 0.21369202189886494 1102 48-49 0.15059898053062426 1102 50-51 0.13526670580875333 1102 52-53 0.181927417608307 1102 54-55 0.1784860808177129 1102 56-57 0.16388700356571206 1102 58-59 0.13831471551485208 1102 60-61 0.1479989980184513 1102 62-63 0.22142991891333708 1102 64-65 0.2802196542321198 1102 66-67 0.2974001633272252 1102 68-69 0.1613286526704556 1102 70-71 0.23847015728829035 1102 72-73 0.1411421024275512 1102 74-75 0.1766705906585031 1102 76-77 0.1339278517119169 1102 78-79 0.11599829505498604 1102 80-81 0.10907676359281027 1102 82-83 0.21419572157159195 1102 84-85 0.20775873197935368 1102 86-87 0.3321152204491362 1102 88-89 1.0363359404710408 1102 90-91 0.08551876121724522 1102 92-93 -0.12814718844980533 1102 94-95 0.010902422804799805 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.10251094003140082 1103 2 0.2756832989476017 1103 3 0.3513687012176092 1103 4 0.27929956941764544 1103 5 0.35128491567387243 1103 6 0.2291801271277123 1103 7 0.32458778812508626 1103 8 0.3127601152296364 1103 9 0.33823599621285894 1103 10-11 0.33776680053819774 1103 12-13 0.35705743759250197 1103 14-15 0.3752438544692822 1103 16-17 0.4489819827471564 1103 18-19 0.4125621314117254 1103 20-21 0.4071690263236576 1103 22-23 0.42636834609073304 1103 24-25 0.3571956535122709 1103 26-27 0.4428830428060948 1103 28-29 0.533831583504373 1103 30-31 0.5040928957492454 1103 32-33 0.503293648155875 1103 34-35 0.4512080888710841 1103 36-37 0.543060908930002 1103 38-39 0.5484451124102847 1103 40-41 0.4766118726746882 1103 42-43 0.4965436260475826 1103 44-45 0.538197455304271 1103 46-47 0.45813674259960635 1103 48-49 0.36177940434645706 1103 50-51 0.40398126039935534 1103 52-53 0.4919883818477544 1103 54-55 0.4178814856257347 1103 56-57 0.4620135714928537 1103 58-59 0.3605135802672308 1103 60-61 0.3940057408821289 1103 62-63 0.4406496910184572 1103 64-65 0.48858918404666696 1103 66-67 0.46794737456135493 1103 68-69 0.4617848899436794 1103 70-71 0.4190635392372073 1103 72-73 0.39007261761515366 1103 74-75 0.4112087467637551 1103 76-77 0.4582589730610849 1103 78-79 0.4500794679950175 1103 80-81 0.5853340267161613 1103 82-83 0.6273374684009312 1103 84-85 0.6808235773435172 1103 86-87 0.7066888810135801 1103 88-89 1.0450291874135047 1103 90-91 -0.5751555748767174 1103 92-93 -0.32156692922747254 1103 94-95 -0.11521921228991605 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.02568819373863107 1104 2 0.09354823428255443 1104 3 0.12561340936967014 1104 4 0.13485457429517567 1104 5 0.0842368306029826 1104 6 0.08928991083615756 1104 7 0.10719863860431644 1104 8 0.13694371826760943 1104 9 0.0099984224303995 1104 10-11 0.19875961660756758 1104 12-13 0.241286395009908 1104 14-15 0.2826887661841795 1104 16-17 0.22116374356566126 1104 18-19 0.21990091001054424 1104 20-21 0.10762051514885229 1104 22-23 0.029933717683562122 1104 24-25 0.2253481032168949 1104 26-27 0.2372891748342454 1104 28-29 0.18990504502749417 1104 30-31 0.12599802144687544 1104 32-33 0.1401275208178987 1104 34-35 0.13143771354909006 1104 36-37 0.135691910195618 1104 38-39 0.1588309268368988 1104 40-41 0.1882265586935219 1104 42-43 0.09594526736358233 1104 44-45 0.0906805229623302 1104 46-47 0.04410166548386485 1104 48-49 0.01935577365102148 1104 50-51 -0.015263042802892812 1104 52-53 0.022054757353487275 1104 54-55 -0.008040258728847505 1104 56-57 -0.13829177275679427 1104 58-59 -0.15664275332921562 1104 60-61 -0.1614438561625846 1104 62-63 -0.2756770394167134 1104 64-65 -0.30025136605650715 1104 66-67 -0.13521643187021226 1104 68-69 -0.22264666326250193 1104 70-71 -0.2194138252768596 1104 72-73 -0.07845262768168482 1104 74-75 -0.07717971011151548 1104 76-77 -0.09010617804563736 1104 78-79 0.012039570163260294 1104 80-81 -0.0783014797672692 1104 82-83 -0.026184567517518076 1104 84-85 0.01739719419812502 1104 86-87 0.053079448463105194 1104 88-89 0.7379802803549289 1104 90-91 -0.612170768352069 1104 92-93 -0.32156692922747254 1104 94-95 -0.11521921228991605 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.16692736085217774 1105 2 -0.22422636132395013 1105 3 -0.06970197654921861 1105 4 0.07502964938593237 1105 5 -0.01812554160564872 1105 6 0.05803315302226508 1105 7 0.08445433336607522 1105 8 -0.02908006734658386 1105 9 0.123899093467287 1105 10-11 0.043078727935686345 1105 12-13 0.20855594631824204 1105 14-15 0.04108984407838534 1105 16-17 0.19283699660208242 1105 18-19 0.1781250646442203 1105 20-21 0.2183750434602416 1105 22-23 -0.03097671154796444 1105 24-25 0.08773862810893007 1105 26-27 0.06762919789618138 1105 28-29 0.17077117017884547 1105 30-31 0.06442135300687823 1105 32-33 0.03407140877626347 1105 34-35 0.14807951343011894 1105 36-37 0.009280051468579131 1105 38-39 0.0823847635262851 1105 40-41 0.0871114647476432 1105 42-43 0.16773023777277984 1105 44-45 0.07756040823399246 1105 46-47 0.19320205348135744 1105 48-49 0.149012564741426 1105 50-51 0.006904595649722012 1105 52-53 0.06527676012196082 1105 54-55 -0.0014019732064625146 1105 56-57 0.07492084499607898 1105 58-59 0.058406099092060515 1105 60-61 0.055381555368342106 1105 62-63 0.1177712459803999 1105 64-65 0.11754738338012061 1105 66-67 0.11864729467817625 1105 68-69 0.215612412799139 1105 70-71 0.07121619027660131 1105 72-73 0.07836505084218004 1105 74-75 0.10246038363938226 1105 76-77 0.1688983910932791 1105 78-79 0.07749325921175654 1105 80-81 -0.08805222972952009 1105 82-83 0.07269688798944429 1105 84-85 0.04331423028210679 1105 86-87 0.09373953813479119 1105 88-89 0.9267073664174816 1105 90-91 -0.7624373703879392 1105 92-93 -0.32156692922747254 1105 94-95 -0.11521921228991605 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.16002097957007422 1106 2 -0.053908787981356454 1106 3 0.034766920298203274 1106 4 0.008852186675461837 1106 5 0.018401297381473114 1106 6 -0.05450035990411095 1106 7 -0.08167951723262235 1106 8 -0.05157245861153825 1106 9 -0.06112172459303622 1106 10-11 -0.15597127633197516 1106 12-13 -0.10116511155023034 1106 14-15 -0.09435253824199208 1106 16-17 -0.26472116944430724 1106 18-19 -0.3095151693411893 1106 20-21 -0.24927260341494417 1106 22-23 -0.2773244602626903 1106 24-25 -0.3792396371579372 1106 26-27 -0.41289477882687464 1106 28-29 -0.16971291256958665 1106 30-31 -0.18237678137688818 1106 32-33 -0.1822760008630695 1106 34-35 -0.2725155755888693 1106 36-37 -0.15044094793861973 1106 38-39 -0.22800345442202996 1106 40-41 -0.22178482144134648 1106 42-43 -0.46688772363575737 1106 44-45 -0.24600989203480594 1106 46-47 -0.39557490155140584 1106 48-49 -0.27454405558883366 1106 50-51 -0.3580387495278714 1106 52-53 -0.35457446762329425 1106 54-55 -0.2054636288064131 1106 56-57 -0.45781934023262494 1106 58-59 -0.23947386120104142 1106 60-61 -0.11038854917211438 1106 62-63 -0.09311003436831555 1106 64-65 -0.09037587333386199 1106 66-67 -0.03417083112347541 1106 68-69 -0.0040266005148339445 1106 70-71 0.0427441458740887 1106 72-73 0.03929608173662302 1106 74-75 -0.04955062578807912 1106 76-77 -0.02670906248553706 1106 78-79 0.014421610241321048 1106 80-81 -0.06970941982336143 1106 82-83 -0.10239276237704331 1106 84-85 -0.16327540780751804 1106 86-87 -0.2564195349967555 1106 88-89 0.808773902364738 1106 90-91 -0.11279708261815502 1106 92-93 -0.30741824817231667 1106 94-95 -0.11521921228991605 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 -0.14776712068535147 1107 2 -0.1432423915210599 1107 3 0.016953565348359234 1107 4 -0.03678229165736724 1107 5 -0.009294855555332049 1107 6 -0.026155711679024307 1107 7 -7.256887461153383E-4 1107 8 0.027729733437638515 1107 9 0.04247539770911857 1107 10-11 0.04407263220316082 1107 12-13 0.008587649621119908 1107 14-15 0.05026697741309505 1107 16-17 0.06326376655055554 1107 18-19 0.08109853687693658 1107 20-21 0.02031398083877889 1107 22-23 0.020118711960094515 1107 24-25 -0.04856108141098758 1107 26-27 -0.009241926460880734 1107 28-29 0.04388860058608657 1107 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122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15747.0 3 2914.0 4 2036.0 5 1740.0 6 1982.0 7 1999.0 8 2190.0 9 2623.0 10 3087.0 11 3803.0 12 4472.0 13 4995.0 14 5539.0 15 6061.0 16 6890.0 17 8181.0 18 10127.0 19 12875.0 20 13733.0 21 19248.0 22 22555.0 23 28847.0 24 44814.0 25 76956.0 26 163179.0 27 448637.0 28 469110.0 29 16522.0 30 637.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.896570743182835 18.7128210580243 13.969828019855882 23.42078017893698 2 14.607443073127119 20.51772005671103 40.2320100521516 24.642826818010246 3 19.788934026892925 25.027750235743163 28.472991079236117 26.710324658127803 4 12.363944497707392 16.7344966151654 37.915929888985424 32.985628998141785 5 13.870466639348592 38.16475368758308 33.74429425849276 14.220485414575574 6 31.31746793968458 36.4000259721912 18.021489847655975 14.26101624046824 7 28.698985871556097 32.259245279518574 21.122455313917456 17.919313535007873 8 25.795665926268946 34.86374232161422 20.09184451790547 19.24874723421137 9 25.936586469201906 18.053241564924413 20.472651068605828 35.537520897267854 10-11 24.9452368550614 26.65841354558363 27.296662837408757 21.099686761946217 12-13 26.343153442294494 23.960347575916284 27.279950166895233 22.416548814893993 14-15 22.749249196752906 25.760739037273662 25.535337520754563 25.954674245218868 16-17 22.50941137184837 28.878962194683393 26.282379685707273 22.329246747760966 18-19 22.565161763583053 28.46042199319679 27.097424926698142 21.876991316522012 20-21 23.616823840302594 27.17667914993876 26.384942155606716 22.821554854151927 22-23 23.030546709830162 27.195961735092506 26.525426812691894 23.24806474238544 24-25 23.428628918037045 27.222531018573687 26.571442434136593 22.77739762925268 26-27 22.93672703298397 27.097058221233123 27.062844853974212 22.9033698918087 28-29 22.598837806674048 27.984363761944447 26.63055266973388 22.78624576164762 30-31 22.757240640200244 27.263380137980835 26.504799503959685 23.474579717859235 32-33 22.685923818113316 27.4157181509938 26.70843417853025 23.189923852362632 34-35 23.486940402340924 27.254088125705493 26.43146832888809 22.827503143065492 36-37 23.21919117972341 26.830184559664016 26.948094110730093 23.00253014988248 38-39 22.861985631099273 27.243972346751583 26.941153721836404 22.952888300312736 40-41 23.12787950615734 27.22167479249004 26.808581383218964 22.841864318133656 42-43 22.764661266258486 27.47526041759573 26.24390028105621 23.51617803508957 44-45 22.871456113387246 27.137855351916308 26.47642023035352 23.514268304342924 46-47 23.355814024840544 27.491671417532228 26.309686985149472 22.842827572477752 48-49 23.13337095976659 27.34072923452565 26.41250748306989 23.113392322637864 50-51 22.512153955212938 27.494535313451994 27.143411141717237 22.849899589617827 52-53 23.11910318879999 27.67576002551554 26.29695062215528 22.908186163529194 54-55 22.5684448771633 27.11922336082462 26.73409327105131 23.57823849096077 56-57 23.030840550011096 27.235160353307425 26.483179795347695 23.250819301333784 58-59 23.652995828038407 26.865734474302155 26.264057270108648 23.217212427550788 60-61 22.866159733257035 27.100982590783158 27.25902765538898 22.77383002057083 62-63 22.824668444287152 27.21732230989819 27.003265788987363 22.9547434568273 64-65 22.592238738664815 27.72656277314504 26.47650836711264 23.204690121077505 66-67 22.70390489040663 27.39017295053368 26.277007689623755 23.62891446943594 68-69 22.63308917730323 27.168212219170325 26.823936875956345 23.374761727570103 70-71 23.122884854002752 27.109437823359134 26.501552980059206 23.26612434257891 72-73 22.93722649819943 26.86384364170078 26.945434852254625 23.25349500784517 74-75 22.481500165180282 27.964664976571513 26.449465893304243 23.104368964943962 76-77 22.85206767896374 27.011364260695153 26.647610879494028 23.488957180847077 78-79 22.893940231787784 27.172649255528462 26.855203095260606 23.07820741742315 80-81 23.13751204959832 27.220176396843666 26.675687959820166 22.966623593737847 82-83 22.854599650015967 27.14516845434421 26.945666851588573 23.05456504405125 84-85 22.702664326790217 27.257860069061795 26.919151179969152 23.120324424178833 86-87 22.687105931527654 27.38779673425091 26.82596430715837 23.099133027063072 88-89 32.070918917009585 17.337736882075554 32.57536956252981 18.015974638385053 90-91 30.943526377350565 13.4324271767639 15.264170164845742 40.35987628103979 92-93 23.01682721036403 16.35149508807993 32.92946434973827 27.70221335181777 94-95 15.066171172390503 6.435302807070461 26.15517581410457 52.34335020643447 96-97 26.066811909949166 6.815229795621952 14.078362209081163 53.03959608534772 98-99 57.33563285649025 14.30631906910375 18.57429564271159 9.78375243169441 100-101 31.167712363057117 6.7918793277201654 11.835307990341962 50.20510031888076 102-103 23.53931747132309 1.9411652008904137 36.0357442615772 38.483773066209295 104-105 43.527860858718306 4.601754858463152 37.21901710916127 14.65136717365728 106-107 5.161491610032692 1.547769231812694 6.0312034307883415 87.25953572736628 108-109 18.278209326597445 9.27520887067182 18.903484586081166 53.54309721664957 110-111 40.329514361383026 2.473815899214258 22.93133166527662 34.2653380741261 112-113 27.905820300101908 2.734841598016268 37.044753642751324 32.314584459130494 114-115 27.64871205760306 3.7897175193103614 22.130308830795066 46.43126159229151 116-117 18.779458427035518 3.912705857108105 19.091344626814777 58.2164910890416 118-119 19.367258458832918 4.13017733434309 14.343929005270727 62.15863520155326 120-121 24.85923382462062 4.1160466165194745 28.10867730718308 42.91604225167683 122-123 33.87286291667774 1.5477339757499535 32.28335414692328 32.29604896064903 124-125 38.313481090586755 1.6464400107888215 17.799838740025884 42.24024015859854 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 27.0 1 66.0 2 150.0 3 233.5 4 292.5 5 328.0 6 304.0 7 252.0 8 241.5 9 235.0 10 211.5 11 182.0 12 171.0 13 188.0 14 204.5 15 234.5 16 279.5 17 341.5 18 447.0 19 633.0 20 927.0 21 1362.5 22 2017.0 23 2896.5 24 4101.5 25 5699.5 26 7566.0 27 9593.0 28 11921.5 29 14427.5 30 17188.5 31 20309.5 32 23297.0 33 26314.0 34 29399.0 35 32288.0 36 34908.5 37 37307.0 38 39632.0 39 42219.0 40 44763.5 41 46859.0 42 49934.0 43 55306.5 44 60378.5 45 62048.0 46 61023.0 47 59240.0 48 58523.5 49 58535.5 50 57965.5 51 55543.0 52 51091.5 53 46213.0 54 41636.0 55 37524.0 56 33516.0 57 29326.5 58 25204.5 59 21078.0 60 17316.5 61 14143.5 62 11427.0 63 9016.0 64 6771.5 65 5053.5 66 3740.5 67 2721.5 68 1988.0 69 1441.5 70 1037.5 71 742.0 72 549.5 73 412.5 74 309.0 75 228.5 76 150.5 77 89.0 78 52.0 79 35.0 80 25.0 81 19.5 82 18.0 83 14.5 84 13.5 85 13.5 86 8.5 87 5.5 88 7.0 89 8.0 90 7.0 91 6.0 92 5.0 93 4.0 94 4.0 95 4.5 96 4.0 97 5.0 98 6.5 99 2.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 5.708173890955326E-4 3 0.04352482591853437 4 0.04937570415676358 5 0.05115950849768713 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.421695627324743E-4 12-13 0.0014983956463757735 14-15 0.0 16-17 2.1405652091082477E-4 18-19 0.007277921710968041 20-21 0.0016767760804681272 22-23 4.2811304182164954E-4 24-25 0.0 26-27 0.0 28-29 7.848739100063574E-4 30-31 0.0 32-33 7.848739100063574E-4 34-35 7.135217363694158E-5 36-37 7.135217363694158E-5 38-39 0.0 40-41 0.0 42-43 0.0 44-45 7.135217363694158E-5 46-47 0.0 48-49 1.4270434727388316E-4 50-51 3.567608681847079E-5 52-53 0.0 54-55 0.001748128254105069 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.2108478136623714E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.0702826045541238E-4 80-81 0.0 82-83 4.637891286401203E-4 84-85 3.567608681847079E-5 86-87 9.632543440987115E-4 88-89 19.199371530054606 90-91 26.24803870712715 92-93 40.296782231025496 94-95 44.645625862023444 96-97 48.417729873514006 98-99 93.04947773776506 100-101 51.71298730858888 102-103 53.07367325984536 104-105 74.8315910321734 106-107 81.58996189080406 108-109 12.594978662132474 110-111 39.044729964131264 112-113 52.003426331378044 114-115 86.78568447069888 116-117 66.41563782778297 118-119 71.92334778690531 120-121 80.38357501503747 122-123 75.83152039352152 124-125 50.13399938209018 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1401499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.91945792379358 #Duplication Level Percentage of deduplicated Percentage of total 1 72.71665605627315 37.75409364472643 2 14.330781881119933 14.880928537837393 3 5.2822432117463345 8.227536125265242 4 2.549502279290401 5.294751052649351 5 1.4208888072948427 3.688588832236691 6 0.8846711684118784 2.7558988502852295 7 0.5725392080389966 2.0808147725051946 8 0.4096222887752953 1.7013893749373572 9 0.30075130987925 1.4053360480921369 >10 1.4174852708632566 12.824196278922722 >50 0.07532626521603815 2.642077671119282 >100 0.03431624614252062 3.280771358508034 >500 0.003294320177697329 1.1313538212182033 >1k 0.0017844234295860532 1.7949151081071917 >5k 1.372633407373887E-4 0.5373485235896222 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7540 0.5379953892225395 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4060 0.28968982496598283 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3018 0.21534086003628972 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2578 0.1839459036360354 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2458 0.17538364279960242 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1891 0.13492696034745655 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1734 0.12372466908645671 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1725 0.12308249952372424 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1433 0.1022476648217373 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.13521736369416E-5 0.0 4 0.0 0.0 0.0 1.427043472738832E-4 0.0 5 0.0 0.0 0.0 1.427043472738832E-4 7.13521736369416E-5 6 0.0 0.0 0.0 1.427043472738832E-4 7.13521736369416E-5 7 0.0 0.0 0.0 1.427043472738832E-4 7.13521736369416E-5 8 0.0 0.0 0.0 1.427043472738832E-4 7.13521736369416E-5 9 7.13521736369416E-5 0.0 0.0 1.427043472738832E-4 7.13521736369416E-5 10-11 7.13521736369416E-5 0.0 0.0 2.140565209108248E-4 7.13521736369416E-5 12-13 7.13521736369416E-5 0.0 0.0 2.854086945477664E-4 7.13521736369416E-5 14-15 7.13521736369416E-5 0.0 0.0 2.854086945477664E-4 7.13521736369416E-5 16-17 7.13521736369416E-5 0.0 0.0 3.5676086818470796E-4 7.13521736369416E-5 18-19 7.13521736369416E-5 0.0 0.0 3.5676086818470796E-4 7.13521736369416E-5 20-21 7.13521736369416E-5 0.0 0.0 3.5676086818470796E-4 7.13521736369416E-5 22-23 7.13521736369416E-5 0.0 0.0 4.994652154585911E-4 7.13521736369416E-5 24-25 7.13521736369416E-5 0.0 0.0 8.205499968248283E-4 7.13521736369416E-5 26-27 7.13521736369416E-5 0.0 0.0 0.0012843391254649486 7.13521736369416E-5 28-29 7.13521736369416E-5 0.0 0.0 0.002568678250929897 7.13521736369416E-5 30-31 7.13521736369416E-5 0.0 0.0 0.006136286932776977 7.13521736369416E-5 32-33 7.13521736369416E-5 0.0 0.0 0.013699617338292785 7.13521736369416E-5 34-35 7.13521736369416E-5 0.0 0.0 0.02561543033566203 7.13521736369416E-5 36-37 7.13521736369416E-5 0.0 0.0 0.043952938960356014 7.13521736369416E-5 38-39 7.13521736369416E-5 0.0 0.0 0.069639721469655 7.13521736369416E-5 40-41 7.13521736369416E-5 0.0 0.0 0.10231901699537424 7.13521736369416E-5 42-43 7.13521736369416E-5 0.0 0.0 0.13481993208700113 7.13521736369416E-5 44-45 7.13521736369416E-5 0.0 0.0 0.17163765368366302 7.13521736369416E-5 46-47 7.13521736369416E-5 0.0 0.0 0.21534086003628972 7.13521736369416E-5 48-49 7.13521736369416E-5 0.0 0.0 0.2627900555048559 7.13521736369416E-5 50-51 7.13521736369416E-5 0.0 0.0 0.3167322987743837 7.13521736369416E-5 52-53 7.13521736369416E-5 0.0 0.0 0.3687837094425326 7.13521736369416E-5 54-55 7.13521736369416E-5 0.0 0.0 0.4223691918438758 7.13521736369416E-5 56-57 7.13521736369416E-5 0.0 0.0 0.49161647635852757 7.13521736369416E-5 58-59 7.13521736369416E-5 0.0 0.0 0.566143821722313 1.427043472738832E-4 60-61 7.13521736369416E-5 0.0 0.0 0.6514453453052766 1.427043472738832E-4 62-63 7.13521736369416E-5 0.0 0.0 0.7374603906246098 1.427043472738832E-4 64-65 7.13521736369416E-5 0.0 0.0 0.8206213489984653 1.427043472738832E-4 66-67 7.13521736369416E-5 0.0 0.0 0.9191587007910815 1.427043472738832E-4 68-69 7.13521736369416E-5 0.0 0.0 1.0331081220892773 1.427043472738832E-4 70-71 7.13521736369416E-5 0.0 0.0 1.1666437150508133 1.427043472738832E-4 72-73 1.070282604554124E-4 0.0 0.0 1.3028906906105533 1.427043472738832E-4 74-75 1.427043472738832E-4 0.0 0.0 1.4363906074852713 1.427043472738832E-4 76-77 1.427043472738832E-4 0.0 0.0 1.5778819678073264 1.427043472738832E-4 78-79 1.427043472738832E-4 0.0 0.0 1.6164834937449117 1.427043472738832E-4 80-81 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 82-83 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 84-85 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 86-87 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 88-89 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 90-91 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 92-93 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 94-95 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 96-97 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 98-99 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 100-101 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 102-103 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 104-105 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 106-107 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 108-109 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 110-111 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 112-113 1.427043472738832E-4 0.0 0.0 1.617268367654918 1.427043472738832E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCGCC 535 0.0 1322.7244 108-109 CCGCTCC 540 0.0 1263.6742 108-109 CCGCTCG 250 0.0 1112.0333 108-109 CGTCGTC 60 2.1623002E-5 1000.40875 110-111 CGTTTCG 65 2.7488333E-5 923.4543 110-111 CTCCGCC 440 0.0 682.0968 110-111 CGTCGCT 140 3.1724085E-7 643.1199 110-111 CCTCGCC 680 0.0 631.8371 108-109 CGTCGCC 285 0.0 620.75226 108-109 CCGTTGC 85 1.02966005E-4 594.6702 108-109 CCGCGCT 130 4.6994865E-7 583.23425 108-109 CGGCTCG 115 1.5204192E-4 521.9524 110-111 GTCGGGT 115 1.5204192E-4 521.9524 110-111 CCGTTCC 245 1.8189894E-11 515.7854 108-109 TTATGCG 60 3.6733974E-5 500.20438 110-111 CCGCACC 310 0.0 489.16425 108-109 CGCCGCC 495 0.0 485.04666 110-111 CTTGTCG 125 1.9520559E-4 480.19617 110-111 CCTCTCG 300 6.184564E-11 421.22473 108-109 TTTCGCG 75 7.79764E-5 400.1635 110-111 >>END_MODULE