FastQCFastQC Report
Fri 27 May 2016
SRR939980_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939980_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1401499
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76890.5486268630944439No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52320.3733145724684784No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40710.2904746988759892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35980.2567251207457158No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30860.22019280784360173No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25960.18523024276150035No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23950.1708884558604751No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22720.1621121385031313No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT19990.14263299510024624No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC18480.13185881688106807No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC16850.12022841257824657No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG15930.11366401260364796No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG71150.027.889061
GAGTACT60400.027.6288412-13
AGTACTT62750.027.0681812-13
TACATGG70250.027.0549552
TAGGACG5600.026.5983334
GTAGGAC8250.025.9986613
GTACTTT63600.025.86592314-15
ACATGGG70250.025.5283433
CATGGGG32950.025.1340124
GTAAGGT4800.024.8251114
TACTTTT72650.023.17612314-15
ACACCGT3256.0936145E-1021.9666886
ACTTTTT76350.021.6255516-17
AGAGTAC120000.020.82259210-11
CGTTTAA2306.062046E-620.7265341
GTCCTAC8500.020.330291
ATAACGC1057.1569644E-519.8310464-65
AGGACGT7300.019.5880345
GTATAGG3051.6201375E-719.5373061
TAAGGTG7400.019.323335
Produced by FastQC (version 0.11.5)