##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939980_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1401499 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.84975016036401 34.0 31.0 34.0 31.0 34.0 2 32.91017260804325 34.0 31.0 34.0 31.0 34.0 3 32.95112661514564 34.0 33.0 34.0 31.0 34.0 4 36.36508409923946 37.0 37.0 37.0 35.0 37.0 5 36.258944173345824 37.0 37.0 37.0 35.0 37.0 6 36.39336667382567 37.0 37.0 37.0 35.0 37.0 7 36.385712012637896 37.0 37.0 37.0 35.0 37.0 8 36.408366327767624 37.0 37.0 37.0 35.0 37.0 9 38.235783257783275 39.0 39.0 39.0 37.0 39.0 10-11 38.23010612208785 39.0 39.0 39.0 37.0 39.0 12-13 38.217521382462635 39.0 39.0 39.0 37.0 39.0 14-15 39.744329821141505 41.0 40.0 41.0 37.0 41.0 16-17 39.774388351329534 41.0 40.0 41.0 37.5 41.0 18-19 39.82082541621507 41.0 40.0 41.0 37.5 41.0 20-21 39.83410869362019 41.0 40.0 41.0 37.5 41.0 22-23 39.7847668817459 41.0 40.0 41.0 37.0 41.0 24-25 39.73953959296439 41.0 40.0 41.0 37.0 41.0 26-27 39.63512424910756 41.0 40.0 41.0 37.0 41.0 28-29 39.551187335845405 41.0 40.0 41.0 37.0 41.0 30-31 39.425804799004496 41.0 39.0 41.0 36.0 41.0 32-33 39.26046076379647 41.0 39.0 41.0 35.0 41.0 34-35 39.13844819011644 41.0 39.0 41.0 35.0 41.0 36-37 39.11874143327965 41.0 39.0 41.0 35.0 41.0 38-39 39.16609394655294 41.0 39.0 41.0 35.0 41.0 40-41 39.11371003475564 41.0 39.0 41.0 35.0 41.0 42-43 38.97928682075406 41.0 39.0 41.0 35.0 41.0 44-45 38.79516717457523 41.0 39.0 41.0 35.0 41.0 46-47 38.5508184451077 41.0 39.0 41.0 35.0 41.0 48-49 38.28415325305262 41.0 39.0 41.0 35.0 41.0 50-51 38.08955125904478 41.0 38.5 41.0 34.0 41.0 52-53 37.954818376609616 41.0 38.0 41.0 34.0 41.0 54-55 37.81033093851654 40.5 38.0 41.0 34.0 41.0 56-57 37.627625492419185 40.0 37.0 41.0 34.0 41.0 58-59 37.42890647799249 40.0 37.0 41.0 33.0 41.0 60-61 37.1843454758084 40.0 36.0 41.0 33.0 41.0 62-63 36.93824076934767 39.0 36.0 41.0 33.0 41.0 64-65 36.64716956630008 39.0 35.0 41.0 32.5 41.0 66-67 36.33236199240956 38.5 35.0 41.0 32.0 41.0 68-69 35.97755010884774 37.0 35.0 40.5 32.0 41.0 70-71 35.59500898680626 37.0 35.0 39.5 32.0 41.0 72-73 35.22261664118204 36.5 35.0 39.0 31.0 41.0 74-75 34.86665563086381 36.0 35.0 39.0 31.0 41.0 76-77 34.49818444394181 35.5 35.0 37.5 31.0 39.5 78-79 34.155727189245226 35.0 35.0 37.0 31.0 39.0 80-81 33.90133992246873 35.0 35.0 37.0 31.0 39.0 82-83 33.706294474701735 35.0 35.0 36.0 31.0 38.0 84-85 33.48896324578183 35.0 35.0 36.0 31.0 37.0 86-87 33.32591924789101 35.0 35.0 36.0 31.0 37.0 88-89 33.16089487042089 35.0 35.0 35.0 31.0 36.0 90-91 33.034392461214736 35.0 34.5 35.0 30.5 36.0 92-93 32.92230961277889 35.0 34.0 35.0 30.0 36.0 94-95 32.84231383682757 35.0 34.0 35.0 30.0 36.0 96-97 32.78851822227487 35.0 34.0 35.0 30.0 36.0 98-99 32.73517533726388 35.0 34.0 35.0 30.0 35.0 100-101 32.68274397627113 35.0 34.0 35.0 30.0 35.0 102-103 31.832069805258513 34.5 32.5 35.0 28.0 35.0 104-105 32.51795149336532 35.0 34.0 35.0 29.0 35.0 106-107 32.57938107697544 35.0 34.0 35.0 30.0 35.0 108-109 32.59133149577703 35.0 34.0 35.0 30.0 35.0 110-111 32.566144892004914 35.0 34.0 35.0 30.0 35.0 112-113 32.497496608987944 35.0 34.0 35.0 29.0 35.0 114-115 32.44260894941773 35.0 34.0 35.0 29.0 35.0 116-117 32.386016686419325 35.0 34.0 35.0 29.0 35.0 118-119 32.31693672275185 35.0 34.0 35.0 29.0 35.0 120-121 32.23551854121908 35.0 34.0 35.0 29.0 35.0 122-123 32.08136930529383 35.0 34.0 35.0 29.0 35.0 124-125 30.091036454539033 33.5 30.5 34.5 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1125678726713275 1101 2 0.10163122741269603 1101 3 0.148753332291939 1101 4 0.10886290674382337 1101 5 0.01363946936541538 1101 6 0.06398304096578045 1101 7 0.12788890788116447 1101 8 0.08436031581300796 1101 9 0.1747150842825036 1101 10-11 0.10102061656871086 1101 12-13 0.11396511017677113 1101 14-15 0.21892065918365233 1101 16-17 0.16941572430391005 1101 18-19 0.21826064593295058 1101 20-21 0.24960435753261123 1101 22-23 0.18392229245024083 1101 24-25 0.2259937726743786 1101 26-27 0.18275627651974702 1101 28-29 0.22163906564761504 1101 30-31 0.19615908330155207 1101 32-33 0.3038314304528811 1101 34-35 0.26651439181353 1101 36-37 0.2308041527996636 1101 38-39 0.193187968712472 1101 40-41 0.30017093220305924 1101 42-43 0.2775303959829287 1101 44-45 0.34716959656615387 1101 46-47 0.3602789452154056 1101 48-49 0.3746122981004021 1101 50-51 0.4403624303885607 1101 52-53 0.3912652876508602 1101 54-55 0.43491791436072447 1101 56-57 0.49436817294281354 1101 58-59 0.5041632939277321 1101 60-61 0.41257353688011733 1101 62-63 0.37728337889365093 1101 64-65 0.3845384271246388 1101 66-67 0.40725346583164423 1101 68-69 0.35678721041698225 1101 70-71 0.4480036453821654 1101 72-73 0.3956069940239928 1101 74-75 0.42534030887241414 1101 76-77 0.41125119211316985 1101 78-79 0.44727426279993665 1101 80-81 0.45887194517930396 1101 82-83 0.4332665427437803 1101 84-85 0.3990555928184065 1101 86-87 0.46011891552599593 1101 88-89 0.37169508460713985 1101 90-91 0.37839125012198593 1101 92-93 0.41455839687228035 1101 94-95 0.38804208193543843 1101 96-97 0.39910194666430243 1101 98-99 0.38046498543332063 1101 100-101 0.3945137974985542 1101 102-103 0.4436311035065792 1101 104-105 0.4454198482426577 1101 106-107 0.45912169534380354 1101 108-109 0.3827971529577425 1101 110-111 0.40226056063635696 1101 112-113 0.42477152961857456 1101 114-115 0.4489647849360807 1101 116-117 0.476565865707677 1101 118-119 0.5232300669903225 1101 120-121 0.5358603126861254 1101 122-123 0.5121146302606334 1101 124-125 0.6231057906624748 1102 1 0.017619401731629125 1102 2 0.053283402827361215 1102 3 0.052362931411039426 1102 4 -0.011938723007780538 1102 5 -0.08302875317023961 1102 6 -0.019754538958736134 1102 7 -0.01643741015910649 1102 8 -0.04605866666899061 1102 9 0.05903715627336226 1102 10-11 0.11056610135734246 1102 12-13 0.007213536065684423 1102 14-15 0.04730979132873614 1102 16-17 0.11711859125070134 1102 18-19 0.004925866775771226 1102 20-21 0.058434489652803734 1102 22-23 0.13007123736282722 1102 24-25 0.11040460030356769 1102 26-27 0.06136624386681433 1102 28-29 0.10915875314650947 1102 30-31 -0.0362468573075958 1102 32-33 0.037862399187019946 1102 34-35 -0.07633962382676174 1102 36-37 0.015338476471541185 1102 38-39 -0.028119707850301268 1102 40-41 -0.15762658710859512 1102 42-43 -0.14095142295150964 1102 44-45 -0.18375863250110314 1102 46-47 -0.15369124304812942 1102 48-49 -0.2567593790230944 1102 50-51 -0.20587791124573585 1102 52-53 -0.2212027082595185 1102 54-55 -0.3196250029901222 1102 56-57 -0.2341188062622166 1102 58-59 -0.3052947303692264 1102 60-61 -0.31795825033592706 1102 62-63 -0.3336572835814806 1102 64-65 -0.23049078478116058 1102 66-67 -0.26978838365809565 1102 68-69 -0.41666141974957327 1102 70-71 -0.38997553983345057 1102 72-73 -0.365548408845342 1102 74-75 -0.37931538781744933 1102 76-77 -0.29083892063569294 1102 78-79 -0.2730327225100524 1102 80-81 -0.29516967423143825 1102 82-83 -0.2951150470766777 1102 84-85 -0.29624274548303475 1102 86-87 -0.251045350209651 1102 88-89 -0.32391002568603255 1102 90-91 -0.24497172260019795 1102 92-93 -0.23360180921348928 1102 94-95 -0.2808380580826082 1102 96-97 -0.3463510727796546 1102 98-99 -0.2614441064454027 1102 100-101 -0.2839715056343337 1102 102-103 -0.28798276604117135 1102 104-105 -0.1992663174071012 1102 106-107 -0.3379454577562413 1102 108-109 -0.32816471436946415 1102 110-111 -0.322193687419265 1102 112-113 -0.3204594102668139 1102 114-115 -0.30624145066175856 1102 116-117 -0.368198110321444 1102 118-119 -0.3536781792338104 1102 120-121 -0.279167350122691 1102 122-123 -0.4538840877981265 1102 124-125 -0.33038765157829886 1103 1 0.08289091885109201 1103 2 0.1275849371272031 1103 3 0.16009609103441136 1103 4 0.06654104964433571 1103 5 -0.06697864911830465 1103 6 0.05325336998276953 1103 7 0.07751738381308826 1103 8 0.08829164896764041 1103 9 0.12901425738896677 1103 10-11 0.16061843811265675 1103 12-13 0.1584523383620322 1103 14-15 0.1858630840689699 1103 16-17 0.2524797148624103 1103 18-19 0.19536576901419522 1103 20-21 0.18057989881186387 1103 22-23 0.14524504263031446 1103 24-25 0.1901343636320263 1103 26-27 0.18241581336280888 1103 28-29 0.11398156650195546 1103 30-31 0.07692037416506992 1103 32-33 0.1557200481851666 1103 34-35 0.21147598172566262 1103 36-37 0.23061956587167742 1103 38-39 0.19239791633449954 1103 40-41 0.19871113289846676 1103 42-43 0.16744595349769753 1103 44-45 0.2823750114548105 1103 46-47 0.1836621580950748 1103 48-49 0.11806962523594677 1103 50-51 0.1771072977159065 1103 52-53 0.22704034313492372 1103 54-55 0.24545842503005133 1103 56-57 0.2206920728212367 1103 58-59 0.1655916130941506 1103 60-61 0.17449705208598942 1103 62-63 0.14420128535012822 1103 64-65 0.22258353341139525 1103 66-67 0.23348869284307483 1103 68-69 0.2105586656302023 1103 70-71 0.2303336075374034 1103 72-73 0.22914680725200043 1103 74-75 0.24312315689753916 1103 76-77 0.22871339498601628 1103 78-79 0.1982305450438986 1103 80-81 0.10588215780362731 1103 82-83 0.14848308620427986 1103 84-85 0.1223082101289279 1103 86-87 0.15148919080570522 1103 88-89 0.09396937849544429 1103 90-91 0.05293654760166788 1103 92-93 0.14928792557218173 1103 94-95 0.11994500420185972 1103 96-97 0.04389800542215028 1103 98-99 0.0871735070482842 1103 100-101 0.15912849610608504 1103 102-103 0.18361913648069006 1103 104-105 0.1692254641062121 1103 106-107 0.20581888512533908 1103 108-109 0.2550124374667959 1103 110-111 0.1769375970316176 1103 112-113 0.2152929336987981 1103 114-115 0.21302978799135275 1103 116-117 0.29016495509576856 1103 118-119 0.28420816174325836 1103 120-121 0.2534766132490347 1103 122-123 0.26912531292737896 1103 124-125 0.2964372353569047 1104 1 0.061460732803844564 1104 2 0.04865381123374135 1104 3 0.0720991662354038 1104 4 0.03650489427415948 1104 5 -0.023060814040462674 1104 6 0.017710864153308137 1104 7 -0.023525936146825188 1104 8 -0.09367176532744992 1104 9 -0.08315043509697517 1104 10-11 0.03944089239865889 1104 12-13 0.08340038994901278 1104 14-15 0.06642609728242377 1104 16-17 0.0018757352331988386 1104 18-19 0.05149676967540984 1104 20-21 0.10599237344206358 1104 22-23 0.04832701293480568 1104 24-25 0.11505026099478499 1104 26-27 0.0896502720537029 1104 28-29 0.07199189695308661 1104 30-31 0.005286323083367961 1104 32-33 0.12324222674264718 1104 34-35 0.06980362360013714 1104 36-37 0.14990013323658502 1104 38-39 0.14572581810214302 1104 40-41 0.1411827531691685 1104 42-43 0.186390231194153 1104 44-45 0.21804287040655623 1104 46-47 0.06568178184230078 1104 48-49 0.06930327160959848 1104 50-51 0.1797427352974239 1104 52-53 0.07741235300360927 1104 54-55 0.09406400753525901 1104 56-57 0.03196877684402466 1104 58-59 0.028808147618242685 1104 60-61 -0.013912800645243806 1104 62-63 0.044158251877099985 1104 64-65 0.06199663420279222 1104 66-67 0.11507798185999008 1104 68-69 0.07492973021304294 1104 70-71 0.1262479660908511 1104 72-73 0.06162426605288829 1104 74-75 0.005372405322766838 1104 76-77 -0.049218169703806325 1104 78-79 -0.0012549873684122304 1104 80-81 0.019077236031471045 1104 82-83 0.11491274642116878 1104 84-85 0.0948886349756819 1104 86-87 0.10127650149166101 1104 88-89 0.054253955467942205 1104 90-91 0.06891743880554912 1104 92-93 0.048001031319685694 1104 94-95 0.13021755356040643 1104 96-97 0.12111577051015843 1104 98-99 0.09397811453103344 1104 100-101 0.1747373170010107 1104 102-103 0.11433258552893832 1104 104-105 0.1543045743770719 1104 106-107 0.13437256152570853 1104 108-109 0.12074425431342917 1104 110-111 0.1294695004287263 1104 112-113 0.04189801575855512 1104 114-115 0.044988064863403565 1104 116-117 0.006245451866448093 1104 118-119 0.04072779334771326 1104 120-121 -0.02842345964192816 1104 122-123 0.08265039503548621 1104 124-125 -0.03575953882494787 1105 1 0.006345568428997694 1105 2 0.09187144173355932 1105 3 -0.0369712114553451 1105 4 -0.030874601865598095 1105 5 -0.07772433487952668 1105 6 0.019670822207885408 1105 7 -0.02817377590341863 1105 8 0.08180226540557811 1105 9 0.10941065790401439 1105 10-11 0.11155484648227088 1105 12-13 0.13055778902110404 1105 14-15 0.0826726204425654 1105 16-17 0.09714912373905094 1105 18-19 0.011780971865185563 1105 20-21 0.03207196338470908 1105 22-23 0.09591395786052459 1105 24-25 0.06695365134643794 1105 26-27 0.08491699580407897 1105 28-29 0.09300159322695123 1105 30-31 0.06906106096586484 1105 32-33 0.1516086786324493 1105 34-35 0.07521424698401802 1105 36-37 0.153692308199183 1105 38-39 0.09952405515614515 1105 40-41 0.12683426728580827 1105 42-43 0.183937270305627 1105 44-45 0.15465773711262187 1105 46-47 0.2586877827219496 1105 48-49 0.18890278125375914 1105 50-51 0.24983126546596424 1105 52-53 0.3033076222798172 1105 54-55 0.21906232362097455 1105 56-57 0.2869384510249233 1105 58-59 0.2994247533055443 1105 60-61 0.3369517150385519 1105 62-63 0.2871182494002724 1105 64-65 0.47007949365704604 1105 66-67 0.44904427001424096 1105 68-69 0.4836641758672826 1105 70-71 0.49622981290404766 1105 72-73 0.40925613899334223 1105 74-75 0.34641952348959393 1105 76-77 0.43338941823846255 1105 78-79 0.4830724383603382 1105 80-81 0.3396906156935202 1105 82-83 0.359862244354737 1105 84-85 0.32308442658437997 1105 86-87 0.2895792411552094 1105 88-89 0.3656710361017872 1105 90-91 0.3299801647898022 1105 92-93 0.1916900869167435 1105 94-95 0.2627251819875269 1105 96-97 0.3154124178309132 1105 98-99 0.2621911068809766 1105 100-101 0.29881852019262567 1105 102-103 0.3571748863065132 1105 104-105 0.36598429837111013 1105 106-107 0.3133769037793357 1105 108-109 0.34216267498855046 1105 110-111 0.294763826192991 1105 112-113 0.2135458387350866 1105 114-115 0.31504206592644834 1105 116-117 0.21258805385788548 1105 118-119 0.25636808373253217 1105 120-121 0.06442036552120811 1105 122-123 0.06755755995485657 1105 124-125 -0.01067796056358361 1106 1 2.6138356282956465E-5 1106 2 0.07199250334355867 1106 3 -0.06101539882607199 1106 4 0.02364708551390038 1106 5 -0.07691663389257997 1106 6 0.021929999751272078 1106 7 0.01475223433666173 1106 8 -0.08462856847436484 1106 9 -0.02874362607982306 1106 10-11 0.03716567912284319 1106 12-13 0.052311814372245635 1106 14-15 -0.039092512550993774 1106 16-17 -0.06874480178362319 1106 18-19 -0.019164452195376214 1106 20-21 -0.01169600614995403 1106 22-23 -0.03129801220261896 1106 24-25 0.004986920489287172 1106 26-27 0.02124798951714979 1106 28-29 -0.01621751612720601 1106 30-31 0.04978497525227965 1106 32-33 0.0922689636662426 1106 34-35 0.11596117162859798 1106 36-37 0.11632257453705819 1106 38-39 0.02109416133351516 1106 40-41 0.05814647739095591 1106 42-43 0.07434716569502342 1106 44-45 -0.017685987127869396 1106 46-47 0.17120469264598626 1106 48-49 0.16384487536004144 1106 50-51 0.1934303988360142 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-0.17359426618656215 2216 18-19 -0.18761283794008676 2216 20-21 -0.21960728287477593 2216 22-23 -0.23235485228522634 2216 24-25 -0.11808212626851855 2216 26-27 -0.12945315746630115 2216 28-29 -0.2920265576410017 2216 30-31 -0.25039272775690335 2216 32-33 -0.34094570960710513 2216 34-35 -0.3339791155670184 2216 36-37 -0.1974676383046443 2216 38-39 -0.15977104275221876 2216 40-41 -0.15924841754704744 2216 42-43 -0.2081381309838335 2216 44-45 -0.23283208254203913 2216 46-47 -0.2613467283082258 2216 48-49 -0.34737261513279805 2216 50-51 -0.4916608258697792 2216 52-53 -0.47505706697109673 2216 54-55 -0.5076284308549717 2216 56-57 -0.6051907460692831 2216 58-59 -0.5824532786668613 2216 60-61 -0.6253966693168138 2216 62-63 -0.6096809683133984 2216 64-65 -0.5497262149630799 2216 66-67 -0.5242852681045207 2216 68-69 -0.5998759421838713 2216 70-71 -0.6976053240641633 2216 72-73 -0.6791773544108821 2216 74-75 -0.6581027289067052 2216 76-77 -0.6975413989947157 2216 78-79 -0.6473047587556593 2216 80-81 -0.6012197999914335 2216 82-83 -0.531598931417264 2216 84-85 -0.529216808131487 2216 86-87 -0.5141648106630896 2216 88-89 -0.5132965747379927 2216 90-91 -0.4772306521165177 2216 92-93 -0.47879698983284413 2216 94-95 -0.5096967732380264 2216 96-97 -0.5395677117693083 2216 98-99 -0.4434702546567948 2216 100-101 -0.4133616671529623 2216 102-103 -0.3729417756842288 2216 104-105 -0.4277224096252681 2216 106-107 -0.3855327437832372 2216 108-109 -0.42696547887562275 2216 110-111 -0.4682881255732809 2216 112-113 -0.48974305971750454 2216 114-115 -0.5406455806500929 2216 116-117 -0.7221378871601232 2216 118-119 -0.6031022931649659 2216 120-121 -0.6542963525806016 2216 122-123 -0.6683390867279115 2216 124-125 -0.7541502822815609 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 104.0 3 2.0 4 18.0 5 51.0 6 115.0 7 342.0 8 558.0 9 969.0 10 1955.0 11 3071.0 12 3706.0 13 6122.0 14 10163.0 15 11108.0 16 4199.0 17 3302.0 18 3228.0 19 2655.0 20 2445.0 21 2404.0 22 2432.0 23 2472.0 24 3144.0 25 4084.0 26 5097.0 27 6651.0 28 8450.0 29 11018.0 30 13912.0 31 18139.0 32 23857.0 33 31370.0 34 45467.0 35 78498.0 36 196093.0 37 558304.0 38 333754.0 39 2240.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.06212979746799 18.747198949516864 14.290995247134722 22.899676005880423 2 14.800010560121699 20.783675193489255 39.85803771533194 24.558276531057103 3 19.718958058478815 25.40800956689944 28.8347690579872 26.03826331663455 4 12.365331691281977 16.919812286701596 37.88201061863048 32.832845403385946 5 13.487976989206905 37.954734294926524 33.68264852742498 14.874640188441592 6 30.923817997729575 36.85461067043216 17.507682845296358 14.71388848654191 7 27.907761618096057 32.8912828335946 21.07472070975434 18.126234838555007 8 25.867731621642253 34.24076649359008 19.903760188198493 19.987741696569174 9 26.12973680323711 17.30026207653377 20.741862819738007 35.82813830049112 10-11 24.89013549064252 26.444435565062836 27.134946225434337 21.530482718860306 12-13 26.84286610265152 23.733623784248152 27.57308424765198 21.850425865448354 14-15 22.905688837451898 25.110221270225665 26.76437871165088 25.21971118067155 16-17 21.751975386336618 28.443101595578447 27.318519184472613 22.48640383361232 18-19 21.7989452721693 27.23965554024655 29.466057414240037 21.495341773344112 20-21 22.868521973299597 25.84496155560051 29.097617128673768 22.188899342426126 22-23 22.156681146432096 25.966895374656794 29.319562810025857 22.55686066888525 24-25 22.677361881813688 25.972512288628103 29.29199378665272 22.05813204290549 26-27 22.217283066202686 25.83383933916471 29.839764423663524 22.10911317096908 28-29 21.74692953758797 26.751570996483053 29.42324611005787 22.078253355871105 30-31 22.035406375602122 26.002123440687434 29.254462543319686 22.708007640390754 32-33 21.982641443197604 26.194631605159902 29.42535099918016 22.39737595246233 34-35 22.78927776616323 26.016857664543462 29.119357202538136 22.074507366755167 36-37 22.524489849796538 25.671370439793396 29.56609316167903 22.238046548731038 38-39 22.263804683413973 26.08503466645356 29.424922886138344 22.22623776399412 40-41 22.498481982505876 26.091242305559977 29.226100054299003 22.184175657635148 42-43 22.308043031068877 26.34960852629934 28.5427246112912 22.799623831340586 44-45 22.419709182810692 26.095238027283646 28.534626139583402 22.95042665032226 46-47 23.02420479786286 26.505762758303785 28.091101028256176 22.378931415577178 48-49 22.991382797989868 26.543900495112734 27.801589583724283 22.663127123173116 50-51 22.392452652481378 26.86016900475848 28.250216375466554 22.497161967293593 52-53 22.92898532214436 27.208795725148573 27.331057674675474 22.531161278031593 54-55 22.40483225460739 26.64614815993447 27.713184240588113 23.23583534487003 56-57 22.873901444096642 26.643900566464907 27.521675006546566 22.96052298289189 58-59 23.414643892004204 26.289137559142034 27.412078067840216 22.884140481013542 60-61 22.684176014396016 26.504621123525595 28.435161209533504 22.37604165254488 62-63 22.595699319086208 26.49923403441601 28.218714390805843 22.68635225569194 64-65 22.31103982236163 27.065520560485595 27.755603107815276 22.8678365093375 66-67 22.488135917328517 26.796594218047957 27.47511771324846 23.240152151375064 68-69 22.396769458986412 26.621531660029724 27.91546765284884 23.066231228135017 70-71 22.90986293960966 26.66209537074233 27.506441317475073 22.921600372172936 72-73 22.841400529005014 26.36994389578587 27.899698822475077 22.88895675273404 74-75 22.381607122088564 27.464058126334734 27.35067952242563 22.803655229151072 76-77 22.66091520579037 26.578078186284827 27.53640923040259 23.224597377522212 78-79 22.781464703149982 26.722280929205088 27.596880197559898 22.89937417008503 80-81 22.949748804672712 26.827275652711847 27.37240625929808 22.850569283317363 82-83 22.720744003384947 26.656458549025007 27.745078662203827 22.877718785386218 84-85 22.595734995173025 26.874653496006772 27.48275239582761 23.04685911299259 86-87 22.60947028859814 26.935837984900452 27.416394874345258 23.038296852156158 88-89 22.530804517163407 26.901196504599717 27.02916662801757 23.5388323502193 90-91 22.640118730779385 26.70925229577093 27.29516443214008 23.355464541309605 92-93 22.880394491897604 26.89973378504016 27.34129671159237 22.878575011469863 94-95 22.517852453963215 26.80640148185357 27.38211101343715 23.293635050746065 96-97 22.288706592013266 26.726526383536488 27.780469340327752 23.204297684122498 98-99 22.59893455641956 26.931812181676257 26.95485921331847 23.51439404858571 100-101 22.263091161677604 26.492098817052312 27.46719762197476 23.777612399295325 102-103 22.672487113769858 26.18787352942855 27.4187011593326 23.720938197468993 104-105 22.969975161847568 26.375891651141337 27.27273375940885 23.381399427602247 106-107 22.551480000970443 26.52160169200053 27.536359601489345 23.390558705539675 108-109 22.2743134030755 26.615319135880206 27.324204751281755 23.786162709762536 110-111 22.174992837695946 27.099232964508868 27.24729553896383 23.47847865883136 112-113 22.520419070834162 26.488490679941144 26.959845467273112 24.03124478195158 114-115 22.069187950299302 26.791653376660534 26.965582987079618 24.173575685960543 116-117 22.409259092107504 26.686355425866935 26.923070062180848 23.981315419844716 118-119 22.189094946332386 26.515183407456455 27.362527354678605 23.93319429153255 120-121 21.865491157099417 27.310761428092857 27.079186709302196 23.744560705505528 122-123 21.94212636672043 26.896087684166325 26.813318306410096 24.34846764270315 124-125 22.063790953018373 26.992780509802465 26.807120166908792 24.13630837027037 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 138.0 1 111.5 2 171.0 3 263.0 4 167.0 5 99.5 6 328.5 7 799.5 8 1332.0 9 1502.0 10 1143.5 11 836.0 12 858.5 13 1011.0 14 1160.5 15 1288.0 16 1414.0 17 1536.0 18 1747.5 19 1923.0 20 2057.0 21 2357.5 22 2850.0 23 3480.0 24 4074.0 25 5071.0 26 6418.0 27 8259.0 28 10271.0 29 12367.5 30 15116.5 31 17933.5 32 21146.0 33 23920.5 34 26120.0 35 28494.0 36 31096.0 37 33535.5 38 35513.0 39 36492.5 40 37467.0 41 40387.0 42 43222.0 43 44896.5 44 47063.0 45 49672.5 46 51394.5 47 58225.0 48 73662.5 49 71706.5 50 58255.0 51 53442.5 52 49653.5 53 47914.5 54 47112.5 55 43788.0 56 39206.0 57 35378.0 58 32099.5 59 28434.5 60 23702.5 61 19683.5 62 15896.5 63 11738.0 64 8469.5 65 6390.0 66 4686.5 67 3719.0 68 2776.0 69 1820.5 70 1314.0 71 1016.0 72 776.0 73 591.0 74 543.0 75 465.0 76 284.0 77 122.0 78 59.5 79 42.5 80 20.5 81 13.0 82 18.5 83 12.5 84 5.0 85 3.0 86 3.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 2.0 99 3.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01705316949922904 2 0.0 3 0.0 4 0.0 5 0.1290047299355904 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.135217363694158E-5 18-19 0.0 20-21 0.0042454543313980245 22-23 2.1405652091082477E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 6.421695627324743E-4 92-93 0.0 94-95 7.848739100063574E-4 96-97 0.0 98-99 0.0012129869518280071 100-101 0.0 102-103 2.1405652091082477E-4 104-105 0.0018551565145604812 106-107 0.005779526064592269 108-109 2.4973260772929556E-4 110-111 0.004602215199582732 112-113 0.0080627956209744 114-115 0.004994652154585911 116-117 0.0016054239068311858 118-119 1.4270434727388316E-4 120-121 5.351413022770619E-4 122-123 0.0010346065177356532 124-125 0.0010702826045541239 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1401499.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.652772010741444 #Duplication Level Percentage of deduplicated Percentage of total 1 71.24885883809883 35.37703343913622 2 14.911991709275805 14.808434491334763 3 5.707356093367537 8.501581526642827 4 2.6545531691392554 5.272236931906506 5 1.5176718386656065 3.7678306896193074 6 0.9470367420848659 2.8213799664321115 7 0.6093523184916021 2.117922222099616 8 0.4417720521635223 1.75481655894342 9 0.3132699565050059 1.3999249555342155 >10 1.5153466591681006 13.145548569562285 >50 0.08606149407377009 2.9058120195788346 >100 0.04040276920488066 3.7081489098991685 >500 0.003882084398987947 1.2486321630735886 >1k 0.0021567135549933037 2.248251805575383 >5k 2.8756180733244046E-4 0.9224457506617403 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7689 0.5486268630944439 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5232 0.3733145724684784 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4071 0.2904746988759892 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3598 0.2567251207457158 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3086 0.22019280784360173 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2596 0.18523024276150035 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2395 0.1708884558604751 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2272 0.1621121385031313 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1999 0.14263299510024624 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1848 0.13185881688106807 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1685 0.12022841257824657 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1593 0.11366401260364796 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.13521736369416E-5 0.0 12-13 0.0 0.0 0.0 1.427043472738832E-4 0.0 14-15 0.0 0.0 0.0 1.427043472738832E-4 0.0 16-17 0.0 0.0 0.0 1.427043472738832E-4 0.0 18-19 0.0 0.0 0.0 1.427043472738832E-4 0.0 20-21 0.0 0.0 0.0 1.427043472738832E-4 0.0 22-23 0.0 0.0 0.0 2.854086945477664E-4 0.0 24-25 0.0 0.0 0.0 6.064934759140036E-4 0.0 26-27 0.0 0.0 0.0 9.989304309171822E-4 0.0 28-29 0.0 0.0 0.0 0.0021762412959267187 0.0 30-31 0.0 0.0 0.0 0.005708173890955327 0.0 32-33 0.0 0.0 0.0 0.013235828209652665 0.0 34-35 0.0 0.0 0.0 0.025080289033384967 0.0 36-37 0.0 0.0 0.0 0.043382121571260486 0.0 38-39 0.0 0.0 0.0 0.06906890408055946 0.0 40-41 0.0 0.0 0.0 0.10153414308536789 0.0 42-43 0.0 0.0 0.0 0.13382100165608396 0.0 44-45 0.0 0.0 0.0 0.17024628629774263 0.0 46-47 0.0 0.0 0.0 0.21398516873718781 0.0 48-49 0.0 0.0 0.0 0.2614700402925725 0.0 50-51 0.0 0.0 0.0 0.3151982270411895 0.0 52-53 0.0 0.0 0.0 0.36707125727524603 0.0 54-55 0.0 0.0 0.0 0.4206210635897707 0.0 56-57 7.13521736369416E-5 0.0 0.0 0.48993970027805944 0.0 58-59 7.13521736369416E-5 0.0 0.0 0.5643600173813895 0.0 60-61 7.13521736369416E-5 0.0 0.0 0.6500183018325378 0.0 62-63 7.13521736369416E-5 0.0 0.0 0.7358192906309602 7.13521736369416E-5 64-65 7.13521736369416E-5 0.0 0.0 0.819622418567548 7.13521736369416E-5 66-67 7.13521736369416E-5 0.0 0.0 0.9180884181865274 7.13521736369416E-5 68-69 7.13521736369416E-5 0.0 0.0 1.0323232481792708 7.13521736369416E-5 70-71 7.13521736369416E-5 0.0 0.0 1.165858841140807 7.13521736369416E-5 72-73 7.13521736369416E-5 0.0 0.0 1.3028906906105533 7.13521736369416E-5 74-75 7.13521736369416E-5 0.0 0.0 1.4360695227039049 7.13521736369416E-5 76-77 7.13521736369416E-5 0.0 0.0 1.5780960243282371 7.13521736369416E-5 78-79 7.13521736369416E-5 0.0 0.0 1.7537650758223873 7.13521736369416E-5 80-81 7.13521736369416E-5 0.0 0.0 1.9526235837485437 7.13521736369416E-5 82-83 7.13521736369416E-5 0.0 0.0 2.1586173090383936 7.13521736369416E-5 84-85 7.13521736369416E-5 0.0 0.0 2.366822951710989 7.13521736369416E-5 86-87 7.13521736369416E-5 0.0 0.0 2.5741010161263045 7.13521736369416E-5 88-89 7.13521736369416E-5 0.0 0.0 2.8104194152118556 7.13521736369416E-5 90-91 7.13521736369416E-5 0.0 0.0 3.0774192489612906 7.13521736369416E-5 92-93 7.13521736369416E-5 0.0 0.0 3.3661101434963565 7.13521736369416E-5 94-95 7.13521736369416E-5 0.0 0.0 3.644205240246336 7.13521736369416E-5 96-97 7.13521736369416E-5 0.0 0.0 3.9217295196072204 7.13521736369416E-5 98-99 7.13521736369416E-5 0.0 0.0 4.221694057576923 7.13521736369416E-5 100-101 7.13521736369416E-5 0.0 0.0 4.573067836652042 7.13521736369416E-5 102-103 7.13521736369416E-5 0.0 0.0 4.957620376468339 7.13521736369416E-5 104-105 7.13521736369416E-5 0.0 0.0 5.323585675052212 7.13521736369416E-5 106-107 7.13521736369416E-5 0.0 0.0 5.696864571433872 7.13521736369416E-5 108-109 7.13521736369416E-5 0.0 0.0 6.089908019912965 7.13521736369416E-5 110-111 7.13521736369416E-5 0.0 0.0 6.529009296474703 7.13521736369416E-5 112-113 7.13521736369416E-5 0.0 0.0 7.009245101138139 7.13521736369416E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 7115 0.0 27.88906 1 GAGTACT 6040 0.0 27.62884 12-13 AGTACTT 6275 0.0 27.06818 12-13 TACATGG 7025 0.0 27.054955 2 TAGGACG 560 0.0 26.598333 4 GTAGGAC 825 0.0 25.998661 3 GTACTTT 6360 0.0 25.865923 14-15 ACATGGG 7025 0.0 25.528343 3 CATGGGG 3295 0.0 25.134012 4 GTAAGGT 480 0.0 24.825111 4 TACTTTT 7265 0.0 23.176123 14-15 ACACCGT 325 6.0936145E-10 21.966688 6 ACTTTTT 7635 0.0 21.62555 16-17 AGAGTAC 12000 0.0 20.822592 10-11 CGTTTAA 230 6.062046E-6 20.726534 1 GTCCTAC 850 0.0 20.33029 1 ATAACGC 105 7.1569644E-5 19.83104 64-65 AGGACGT 730 0.0 19.588034 5 GTATAGG 305 1.6201375E-7 19.537306 1 TAAGGTG 740 0.0 19.32333 5 >>END_MODULE