FastQCFastQC Report
Fri 27 May 2016
SRR939979_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939979_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310255
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24860.18973405940065102No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19810.15119194355297252No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC18220.1390569011375648No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13940.10639150394388878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCC2550.03199.08108-109
CGGCGTC108.734308E-43058.026110-111
CGTCGAC100.0013372512472.0164108-109
CGTCGCT401.9645086E-92293.5195110-111
CCGCGCG750.01977.6133108-109
CCGCTCG557.2759576E-121797.8302108-109
CCGCGCC2500.01680.9712108-109
CCTCGCG750.01648.0111108-109
CCGTTCG150.00300841081648.0111108-109
TACGTCG150.00300841081648.0111108-109
CATCGCG457.386916E-91648.011108-109
CGGCGTA200.00349296581529.013110-111
CGTCGCC1150.01504.7057108-109
CTCCGCC1850.01322.3895110-111
CCGTCGT200.00534756851236.0082108-109
AAGCTCG1100.01123.6438108-109
CGTCGCG451.6341357E-51098.674108-109
CAGCGCG704.321737E-81059.4357108-109
CGCCGGG300.0078574691019.342110-111
CCGCGCA250.008354454988.8066108-109
Produced by FastQC (version 0.11.5)