FastQCFastQC Report
Fri 27 May 2016
SRR939979_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939979_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310255
Sequences flagged as poor quality0
Sequence length125
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT27730.2116381925655693No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24350.1858416873051429No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA20240.15447374747663623No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17340.1323406512472763No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC17070.1302799836673014No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT13660.10425451534243334No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT953.2880856E-431.3133186
GTACATG50850.029.9981961
TACATGG49400.029.1844522
GTATCAA79150.028.7579941
TAGGACC19550.028.3399684
ACATGGG48400.027.8180243
GTATAGC7600.027.4410421
TCAACGC87950.026.0110874
ATCAACG88050.025.9815463
AGAGTAC66350.025.95914310-11
CAACGCA88950.025.7856435
AACGCAG89600.025.630796
TATCAAC91450.025.211022
GAGTACT40450.025.1512912-13
AGTACTT42150.024.5603412-13
CATGGGG27500.023.6133164
ACGCAGA97800.023.5426247
CGCAGAG98000.023.4945768
GTACTTT44350.022.94131714-15
AGGACCG1300.002037360322.9134145
Produced by FastQC (version 0.11.5)