FastQCFastQC Report
Fri 27 May 2016
SRR939978_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939978_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814691
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19800.2430369305663129No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC19180.23542668324554955No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14570.1788408120379383No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA12050.1479088390567712No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA9660.11857256309447387No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA9380.11513567720767752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGGC52.1769406E-46120.596110-111
CGTCGCG53.4524038E-44862.355108-109
CCGTTCG300.04051.9626108-109
CGGCGCT108.7068224E-43060.298110-111
CGCTACG108.7068224E-43060.298110-111
CCGCTCC2000.02795.8545108-109
CCGCGCC1400.02257.5222108-109
CAGCGCG352.8685463E-92083.8667108-109
CGGCGCG352.8685463E-92083.8667108-109
CGGCGTG150.00195882352040.1987110-111
CGTCGCT150.00195882352040.1987110-111
CGCGTCT150.00195882352040.1987110-111
CGCCGCT150.00195882352040.1987110-111
CGTCGCC556.9267116E-91669.2535110-111
CCGCGCA150.00310631731620.785108-109
CGCCGAC150.00310631731620.785108-109
ACGCGTC150.00310631731620.785108-109
CGCGACT200.0034819771530.149110-111
CCGCGCG551.747867E-81326.0968108-109
CGTGGCG250.0054400021224.1193110-111
Produced by FastQC (version 0.11.5)