##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939978_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 814691 Sequences flagged as poor quality 0 Sequence length 125 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.889172704743274 34.0 31.0 34.0 31.0 34.0 2 32.9621009683426 34.0 33.0 34.0 31.0 34.0 3 33.00968097106756 34.0 33.0 34.0 31.0 34.0 4 36.38134458340647 37.0 37.0 37.0 35.0 37.0 5 36.298881416389776 37.0 37.0 37.0 35.0 37.0 6 36.44022089356578 37.0 37.0 37.0 35.0 37.0 7 36.43785680705936 37.0 37.0 37.0 35.0 37.0 8 36.462834375241655 37.0 37.0 37.0 35.0 37.0 9 38.31261177550753 39.0 39.0 39.0 37.0 39.0 10-11 38.29305528108203 39.0 39.0 39.0 37.0 39.0 12-13 38.28484664737919 39.0 39.0 39.0 37.0 39.0 14-15 39.83429300188661 41.0 40.0 41.0 38.0 41.0 16-17 39.86906876349438 41.0 40.0 41.0 38.0 41.0 18-19 39.92367167429123 41.0 40.0 41.0 38.0 41.0 20-21 39.945794172269 41.0 40.0 41.0 38.0 41.0 22-23 39.90032662690517 41.0 40.0 41.0 38.0 41.0 24-25 39.8798924991193 41.0 40.0 41.0 38.0 41.0 26-27 39.795279437234484 41.0 40.0 41.0 37.0 41.0 28-29 39.71806549968025 41.0 40.0 41.0 37.0 41.0 30-31 39.58884718255142 41.0 40.0 41.0 37.0 41.0 32-33 39.44917459503051 41.0 40.0 41.0 36.0 41.0 34-35 39.367413534702116 41.0 40.0 41.0 35.5 41.0 36-37 39.35423000867814 41.0 39.5 41.0 35.5 41.0 38-39 39.39340928032837 41.0 40.0 41.0 36.0 41.0 40-41 39.291307379116745 41.0 40.0 41.0 35.0 41.0 42-43 39.20071536324815 41.0 40.0 41.0 35.0 41.0 44-45 39.04701598520175 41.0 40.0 41.0 35.0 41.0 46-47 38.80003645553958 41.0 40.0 41.0 35.0 41.0 48-49 38.54901122020496 41.0 40.0 41.0 35.0 41.0 50-51 38.400090954730075 41.0 39.5 41.0 35.0 41.0 52-53 38.31907803081168 41.0 39.0 41.0 35.0 41.0 54-55 38.23958838381668 41.0 39.0 41.0 35.0 41.0 56-57 38.114276455735975 41.0 39.0 41.0 34.5 41.0 58-59 37.988461269364706 41.0 39.0 41.0 34.0 41.0 60-61 37.838241124549064 41.0 38.5 41.0 34.0 41.0 62-63 37.65575844093037 40.0 38.0 41.0 34.0 41.0 64-65 37.44569904417749 40.0 37.0 41.0 34.0 41.0 66-67 37.19261842833663 40.0 37.0 41.0 33.5 41.0 68-69 36.86612040638721 39.0 36.5 41.0 33.0 41.0 70-71 36.493437389145086 39.0 36.0 41.0 32.0 41.0 72-73 36.15293282974772 38.5 35.0 40.5 32.0 41.0 74-75 35.7923089858609 37.0 35.0 39.5 32.0 41.0 76-77 35.38997178071195 37.0 35.0 39.0 31.5 41.0 78-79 34.993368651427346 36.5 35.0 39.0 31.0 40.5 80-81 34.67915811025284 36.0 35.0 38.0 31.0 39.5 82-83 34.390206225427804 36.0 35.0 37.0 31.0 39.0 84-85 34.06851493388291 35.0 35.0 37.0 31.0 39.0 86-87 33.80874650634412 35.0 35.0 36.5 31.0 37.5 88-89 33.55682215711233 35.0 35.0 36.0 31.0 37.0 90-91 33.33622256782019 35.0 35.0 36.0 31.0 37.0 92-93 33.18775400734757 35.0 35.0 36.0 31.0 36.5 94-95 33.07922083342028 35.0 35.0 35.0 31.0 36.0 96-97 32.9427586655554 35.0 34.0 35.0 30.5 36.0 98-99 32.819859308621304 35.0 34.0 35.0 30.0 36.0 100-101 32.80730853783827 35.0 34.0 35.0 30.0 36.0 102-103 32.004352570483775 34.5 33.0 35.0 28.0 35.0 104-105 32.64092889205846 35.0 34.0 35.0 30.0 35.0 106-107 32.694068057705316 35.0 34.0 35.0 30.0 35.0 108-109 32.69499049332815 35.0 34.0 35.0 30.0 35.0 110-111 32.68719244474285 35.0 34.0 35.0 30.0 35.5 112-113 32.618463932951265 35.0 34.0 35.0 30.0 36.0 114-115 32.559913513221574 35.0 34.0 35.0 30.0 36.0 116-117 32.50208729444661 35.0 34.0 35.0 30.0 35.0 118-119 32.425245277043686 35.0 34.0 35.0 29.0 35.0 120-121 32.33887756216774 35.0 34.0 35.0 29.0 35.0 122-123 32.18167992527228 35.0 34.0 35.0 29.0 35.0 124-125 30.184056286371153 33.5 30.5 34.5 23.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.050828888722776355 1101 2 0.10890206744215192 1101 3 0.10865506103149869 1101 4 0.023164017559906824 1101 5 -0.024758846257888933 1101 6 0.03669470638421757 1101 7 0.0457413619240441 1101 8 0.0851982640956237 1101 9 0.11666045293240046 1101 10-11 0.09572946108136904 1101 12-13 0.06151541065082 1101 14-15 0.2563110961111619 1101 16-17 0.23838210822217576 1101 18-19 0.23993732076711183 1101 20-21 0.22532412528480705 1101 22-23 0.2537317000158765 1101 24-25 0.2399147176076326 1101 26-27 0.25372465747327766 1101 28-29 0.271021747306726 1101 30-31 0.3521520532369422 1101 32-33 0.3131696226945806 1101 34-35 0.3260973614866174 1101 36-37 0.36694786920516975 1101 38-39 0.3051734828245358 1101 40-41 0.3366696172840662 1101 42-43 0.39539641799318304 1101 44-45 0.4723612358939562 1101 46-47 0.44303007748228396 1101 48-49 0.48411929509121876 1101 50-51 0.5971931319396973 1101 52-53 0.5868553176198361 1101 54-55 0.5330085922062509 1101 56-57 0.6137632054730489 1101 58-59 0.6271357812181222 1101 60-61 0.6026376519391405 1101 62-63 0.5573914479007627 1101 64-65 0.7061834370091233 1101 66-67 0.6707383016712569 1101 68-69 0.6319687523077491 1101 70-71 0.6925425847654694 1101 72-73 0.6442174195137795 1101 74-75 0.6069980773187069 1101 76-77 0.5927801367204992 1101 78-79 0.6102636185276538 1101 80-81 0.6592164506605798 1101 82-83 0.5956525581493892 1101 84-85 0.574603941115079 1101 86-87 0.5282446768135003 1101 88-89 0.4954847371365787 1101 90-91 0.47589979692838114 1101 92-93 0.534872324292273 1101 94-95 0.47505134550171846 1101 96-97 0.4889686235722266 1101 98-99 0.5080215889854358 1101 100-101 0.36479273587568883 1101 102-103 0.5495495894618045 1101 104-105 0.45817281959583767 1101 106-107 0.4623977430368953 1101 108-109 0.4460857661218114 1101 110-111 0.5128297322885302 1101 112-113 0.5245910516287537 1101 114-115 0.557668652658478 1101 116-117 0.6047539086817437 1101 118-119 0.6028703524742767 1101 120-121 0.6612773230996538 1101 122-123 0.7086379284747579 1101 124-125 0.7483541321808396 1102 1 0.05943530022646115 1102 2 0.10168457778392082 1102 3 0.0880909499493896 1102 4 0.04350191471957743 1102 5 -0.06331566470935002 1102 6 0.012223263914528104 1102 7 0.04393639629115853 1102 8 -0.058663508124361385 1102 9 -0.0014153050028866687 1102 10-11 0.037673160707242914 1102 12-13 -0.024863110099957453 1102 14-15 0.05149772259717622 1102 16-17 0.15166940781324456 1102 18-19 0.07346878902203002 1102 20-21 0.08339268550438561 1102 22-23 0.11662589359487185 1102 24-25 0.18199129813900328 1102 26-27 0.1763472361538021 1102 28-29 0.14058687872436337 1102 30-31 0.08077167373540561 1102 32-33 0.009178054304413763 1102 34-35 0.1648538967751918 1102 36-37 0.22020859261515113 1102 38-39 0.14299787438027067 1102 40-41 0.21987995668404636 1102 42-43 0.14033270634474349 1102 44-45 0.05402643928874795 1102 46-47 0.1269158948185023 1102 48-49 0.13667013390193716 1102 50-51 0.15119616921753476 1102 52-53 0.22844071337555505 1102 54-55 0.21743977492540978 1102 56-57 0.16542896253828587 1102 58-59 0.03980890210019794 1102 60-61 0.08894459101166063 1102 62-63 0.11152736608409697 1102 64-65 0.032868374800294475 1102 66-67 0.17335518545582573 1102 68-69 0.1547811608532541 1102 70-71 0.27542704956389485 1102 72-73 0.14236301902021609 1102 74-75 0.21091134088956665 1102 76-77 0.24364584000059608 1102 78-79 0.17588582545803177 1102 80-81 0.08384054798003149 1102 82-83 0.05147282090555905 1102 84-85 0.13942648973920768 1102 86-87 0.06916764543620957 1102 88-89 0.13073495662736434 1102 90-91 0.19621080152384707 1102 92-93 0.12451156845302336 1102 94-95 0.13517441853085188 1102 96-97 0.16803750808448115 1102 98-99 0.10571080585815196 1102 100-101 0.17498936797750275 1102 102-103 0.06632382638529322 1102 104-105 0.12082458461809864 1102 106-107 0.12382550090890021 1102 108-109 0.13382315232276198 1102 110-111 0.1646520989923701 1102 112-113 0.18327853630394486 1102 114-115 0.2308278961864616 1102 116-117 0.014509581826345652 1102 118-119 0.022072829754016254 1102 120-121 -0.044082602233928014 1102 122-123 0.0409884967779135 1102 124-125 -0.01173888445300264 1103 1 0.01369403858264917 1103 2 0.005963452201250163 1103 3 0.049512262697135156 1103 4 0.029818651103816762 1103 5 -0.08156310218340934 1103 6 0.04074163556295218 1103 7 -0.04351274279579087 1103 8 -0.08730763371783468 1103 9 0.020827713338675835 1103 10-11 0.06829077459171629 1103 12-13 0.0847781285532605 1103 14-15 -0.01538177426360221 1103 16-17 0.021200075574412836 1103 18-19 -0.0016545347087486562 1103 20-21 -0.04135599947966995 1103 22-23 -0.04108614067192207 1103 24-25 0.07879437054704397 1103 26-27 0.029580038337442716 1103 28-29 -0.03571205151845902 1103 30-31 -0.22970247264218813 1103 32-33 -0.11299846103062094 1103 34-35 -0.028877384788657423 1103 36-37 -0.01302331463244144 1103 38-39 -0.12936395443698245 1103 40-41 -0.17333449931056322 1103 42-43 -0.16032723184294895 1103 44-45 -0.24333882268835083 1103 46-47 -0.20179488808081913 1103 48-49 -0.19006253172680942 1103 50-51 -0.22487023195143507 1103 52-53 -0.15861642829128186 1103 54-55 -0.14684780197352154 1103 56-57 -0.20121090747997528 1103 58-59 -0.21149608439090173 1103 60-61 -0.12818772528342492 1103 62-63 -0.13868749571772554 1103 64-65 -0.2712077503117669 1103 66-67 -0.06799093723373772 1103 68-69 -0.12948408893419838 1103 70-71 -0.02267614890669023 1103 72-73 -0.0863031713758815 1103 74-75 -0.014933872205510568 1103 76-77 -0.09719595536455472 1103 78-79 -0.09725945815183223 1103 80-81 -0.27490536887424355 1103 82-83 -0.23681154039555707 1103 84-85 -0.2290194538088386 1103 86-87 -0.1734668765676446 1103 88-89 -0.19994584282827077 1103 90-91 -0.1170291601481992 1103 92-93 -0.13048811523336923 1103 94-95 -0.13133175112742634 1103 96-97 -0.20620546088129998 1103 98-99 -0.09294185248766951 1103 100-101 -0.10182168170533856 1103 102-103 -0.23369191214525387 1103 104-105 -0.25019970055173246 1103 106-107 -0.15355722775112213 1103 108-109 -0.1066588941799651 1103 110-111 -0.04329053560678631 1103 112-113 -0.08225516189584425 1103 114-115 -0.13971651810103936 1103 116-117 -0.21875897150952284 1103 118-119 -0.2015898194727157 1103 120-121 -0.1677130029029712 1103 122-123 -0.23955235319531454 1103 124-125 -0.22817415357415882 1104 1 0.04393734244504088 1104 2 0.022095311837439624 1104 3 0.021327580097874943 1104 4 3.926528448090494E-4 1104 5 0.016846185884226372 1104 6 -9.334588654326126E-4 1104 7 -0.003308199145976687 1104 8 -4.380115662243611E-4 1104 9 -0.04287043345270547 1104 10-11 0.0635412626527625 1104 12-13 0.11730329572259279 1104 14-15 0.07383488588559572 1104 16-17 0.13898347685423573 1104 18-19 0.11325705994363489 1104 20-21 0.1450091555836508 1104 22-23 0.15600699321187506 1104 24-25 0.09025687562242268 1104 26-27 0.07934503488394284 1104 28-29 0.13662968260775443 1104 30-31 0.18040140233028978 1104 32-33 0.20291525067550253 1104 34-35 0.20053228234097276 1104 36-37 0.29881810260993547 1104 38-39 0.20414355263859818 1104 40-41 0.16169899448735947 1104 42-43 0.24274901355549616 1104 44-45 0.366180502731126 1104 46-47 0.3455668853899354 1104 48-49 0.3450591351870713 1104 50-51 0.3219218248269584 1104 52-53 0.3985301638215333 1104 54-55 0.2749972560619227 1104 56-57 0.37730436811907 1104 58-59 0.3403140489999643 1104 60-61 0.4127157607384788 1104 62-63 0.4851961067795898 1104 64-65 0.4201773541999714 1104 66-67 0.4786954915977546 1104 68-69 0.5591213524782503 1104 70-71 0.5705753489874894 1104 72-73 0.5004638193663169 1104 74-75 0.39966068877927086 1104 76-77 0.4093327507155635 1104 78-79 0.3562245410870446 1104 80-81 0.35331796905877866 1104 82-83 0.4396215911209822 1104 84-85 0.5042265304563429 1104 86-87 0.4353355272547361 1104 88-89 0.4582724609217479 1104 90-91 0.4420595628323909 1104 92-93 0.37979525233540556 1104 94-95 0.33479651266376464 1104 96-97 0.38688572225658646 1104 98-99 0.5104581687877499 1104 100-101 0.5099195762710664 1104 102-103 0.5485991505318282 1104 104-105 0.5591635801773833 1104 106-107 0.5227961209544532 1104 108-109 0.44135200422692833 1104 110-111 0.4321000255997447 1104 112-113 0.36147960258379896 1104 114-115 0.4176177321727721 1104 116-117 0.475828307889131 1104 118-119 0.42783175889348257 1104 120-121 0.40051029008198213 1104 122-123 0.42818932129981135 1104 124-125 0.5426630190728474 1105 1 0.040248575968547584 1105 2 0.1017234841766097 1105 3 -0.031102087421302826 1105 4 0.02308266530464209 1105 5 -0.057765264046913956 1105 6 -2.121000878076984E-4 1105 7 -0.00750519567034047 1105 8 0.02497349082737088 1105 9 0.053922798890397416 1105 10-11 0.07359110198648722 1105 12-13 0.08426014535207571 1105 14-15 0.01906080233913343 1105 16-17 0.009130699622943439 1105 18-19 -0.03652325351954744 1105 20-21 0.09212863882845568 1105 22-23 0.07452624037563282 1105 24-25 -0.013684366426232941 1105 26-27 0.08537821738771356 1105 28-29 0.09276615057287074 1105 30-31 0.08680510093346783 1105 32-33 0.10416287347044317 1105 34-35 0.09509913918404322 1105 36-37 0.18076118084456994 1105 38-39 0.21575906841012227 1105 40-41 0.22468673138813955 1105 42-43 0.27880055791905534 1105 44-45 0.2107112916353202 1105 46-47 0.35488716868720616 1105 48-49 0.26571786690718113 1105 50-51 0.24185100051061426 1105 52-53 0.25841441852675473 1105 54-55 0.19936114877619815 1105 56-57 0.1327883342807894 1105 58-59 0.10222466608961156 1105 60-61 0.19262755823339717 1105 62-63 0.2548695279875446 1105 64-65 0.23579641118731587 1105 66-67 0.201915046978435 1105 68-69 0.22179595409146913 1105 70-71 0.2748100529025024 1105 72-73 0.2390696295833763 1105 74-75 0.22276384308447206 1105 76-77 0.22570595551587758 1105 78-79 0.2309376370277647 1105 80-81 0.17615881108120846 1105 82-83 0.17342380765976628 1105 84-85 0.09688014708694226 1105 86-87 -0.025120896117286406 1105 88-89 0.05250814850781893 1105 90-91 0.18209989334587107 1105 92-93 0.0720947473842628 1105 94-95 0.0433593692445271 1105 96-97 0.06740575635718216 1105 98-99 0.027787173968413015 1105 100-101 0.08604751908699626 1105 102-103 0.022695812608027666 1105 104-105 0.10356813129549636 1105 106-107 0.11856469594297891 1105 108-109 0.1301512697525311 1105 110-111 0.15537696179228533 1105 112-113 0.0867976562266577 1105 114-115 0.1227459373009765 1105 116-117 0.036832308151446114 1105 118-119 0.010869990908695115 1105 120-121 -0.14365654052986088 1105 122-123 -0.0020192169649959624 1105 124-125 -0.08507486537859421 1106 1 0.0703681000652665 1106 2 0.024128769554096152 1106 3 0.018505895472493705 1106 4 -0.04314081185967922 1106 5 -0.10428919628004252 1106 6 0.023890098497638235 1106 7 0.024991103468863685 1106 8 0.005564532002871658 1106 9 0.02783310099029279 1106 10-11 0.05794331427883037 1106 12-13 0.09029254844435286 1106 14-15 0.06774219934554537 1106 16-17 0.019621954625954174 1106 18-19 0.02569644054263165 1106 20-21 0.04165849243884878 1106 22-23 -0.0017512419424861037 1106 24-25 -0.02416739729414985 1106 26-27 0.04413966264237956 1106 28-29 0.0040153966938234475 1106 30-31 0.0835469727466176 1106 32-33 -0.03878355999630401 1106 34-35 0.10810149676943581 1106 36-37 0.12254526988454728 1106 38-39 0.07272628152487925 1106 40-41 0.015143993299425063 1106 42-43 0.03642433115223298 1106 44-45 0.040120798735486574 1106 46-47 0.26126224407510534 1106 48-49 0.12606657023522416 1106 50-51 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-0.21529500240181676 2215 110-111 -0.1925480945178677 2215 112-113 -0.17440692594065155 2215 114-115 -0.15765585552317418 2215 116-117 -0.04356379677881961 2215 118-119 -0.08928813254134838 2215 120-121 -0.07717643678267194 2215 122-123 -0.2701909003540699 2215 124-125 -0.2934366381813618 2216 1 -0.001466780440367188 2216 2 -0.11091413430175834 2216 3 -0.007354924858006484 2216 4 0.010638238769068664 2216 5 0.06505265198734378 2216 6 0.016147653307044152 2216 7 0.028258819140290825 2216 8 0.018271095963633854 2216 9 0.031411348406187756 2216 10-11 0.019125748935493903 2216 12-13 -0.019818935743764143 2216 14-15 0.05360477533255903 2216 16-17 -0.022824881900476157 2216 18-19 -0.15406029130278398 2216 20-21 -0.1674632403941203 2216 22-23 -0.04395996755530973 2216 24-25 -0.0997846202844741 2216 26-27 -0.1419390785518999 2216 28-29 -0.12246209798434649 2216 30-31 -0.22013167627569885 2216 32-33 -0.18754856537300668 2216 34-35 -0.16847914272844378 2216 36-37 -0.07305900513905073 2216 38-39 -0.009977210037000361 2216 40-41 -0.07750419508251838 2216 42-43 -0.012539831509336352 2216 44-45 -0.09551025004981284 2216 46-47 -0.08167233577434985 2216 48-49 0.10737266894700781 2216 50-51 0.01569778116724052 2216 52-53 0.007366189937933143 2216 54-55 -0.001744835920675314 2216 56-57 -0.09940297275336718 2216 58-59 -0.10496921534470971 2216 60-61 -0.13710329382590203 2216 62-63 -0.12756097440105663 2216 64-65 -0.15005412585494327 2216 66-67 -0.193164155002286 2216 68-69 -0.22017314862933546 2216 70-71 -0.20803268930378493 2216 72-73 -0.11543198893081552 2216 74-75 -0.12462571514846132 2216 76-77 -0.20469625533421265 2216 78-79 -0.2254106099176525 2216 80-81 -0.16129803973342405 2216 82-83 -0.13226270651286143 2216 84-85 -0.15844718229748622 2216 86-87 -0.16984136103157255 2216 88-89 -0.03711231774963153 2216 90-91 -0.053580285563747054 2216 92-93 0.021982928872759544 2216 94-95 -0.014874122134592938 2216 96-97 -0.07699794540639715 2216 98-99 -0.09898300597096465 2216 100-101 -0.07641895774563778 2216 102-103 0.008330659688652986 2216 104-105 -0.017662051419762292 2216 106-107 -0.10987694128598946 2216 108-109 -0.09051288071779595 2216 110-111 -0.1203245062873961 2216 112-113 -0.10255898997912283 2216 114-115 -0.15154296860133343 2216 116-117 -0.2641632011992243 2216 118-119 -0.1426034345657783 2216 120-121 -0.09666254447876099 2216 122-123 -0.10981606769007612 2216 124-125 -0.16891132604847314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93.0 3 1.0 4 11.0 5 14.0 6 48.0 7 117.0 8 263.0 9 499.0 10 1090.0 11 1797.0 12 2204.0 13 4050.0 14 7129.0 15 7794.0 16 2565.0 17 1445.0 18 1122.0 19 1073.0 20 1039.0 21 1070.0 22 1133.0 23 1185.0 24 1553.0 25 1873.0 26 2477.0 27 3094.0 28 4114.0 29 5125.0 30 6786.0 31 8652.0 32 11444.0 33 15301.0 34 21952.0 35 35419.0 36 75262.0 37 276010.0 38 305918.0 39 3969.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.131464321316855 20.028040090946146 15.109115730441914 22.73137985729509 2 12.732925735033282 20.36477633851362 46.07673338725971 20.825564539193387 3 19.671752848626042 26.490902685803576 29.13116752241034 24.706176943160045 4 11.00515410137095 17.606429922510497 40.176827778875676 31.21158819724288 5 11.366675844707547 39.38817704440861 35.24847087283169 13.996676238052158 6 28.382908366484962 38.04338086464684 20.158194947532255 13.41551582133594 7 27.162568384823203 32.61813374641429 22.086656167798587 18.132641700963923 8 23.852233546215682 39.17963988800662 20.58854215892897 16.379584406848732 9 26.198890131350417 17.39849832635932 22.32416953176112 34.078442010529145 10-11 23.767293366442 26.945921828030507 30.13443133654355 19.152353468983947 12-13 26.59087924133199 24.69230665368833 29.74403792358084 18.97277618139884 14-15 20.46579623440053 29.583056643561793 27.35626145372908 22.5948856683086 16-17 18.751783652810484 30.424621374620177 31.439362555473522 19.384232417095816 18-19 19.722446915456292 27.89542292722026 33.43200059900011 18.95012955832334 20-21 20.999845333490455 27.8552542422814 31.452662227983346 19.692238196244798 22-23 19.49729190640583 30.08300728807058 31.681043344840816 18.738657460682777 24-25 20.242828262494612 28.030750309012863 33.50933053145303 18.217090897039494 26-27 18.171368040152647 29.784359959788436 33.0467625148676 18.99750948519132 28-29 18.306327184171668 29.77748618801484 31.009671151393597 20.906515476419894 30-31 19.6042425901354 28.187496854635686 32.348951933923416 19.8593086213055 32-33 18.484370147700172 29.350698608429454 33.31146410111318 18.853467142757193 34-35 18.752385873908022 29.782518770920507 33.15244675588659 18.31264859928488 36-37 18.186527161831908 29.04058103010835 32.83435069247113 19.93854111558861 38-39 19.664326720192076 29.744528907278955 31.55840680699799 19.03273756553098 40-41 21.494161590099807 27.490422749238668 32.79758828807486 18.21782737258666 42-43 18.664315673058866 30.082448437505754 32.751190328603116 18.50204556083227 44-45 18.573851926681403 30.13535193097751 31.604559274620687 19.6862368677204 46-47 20.51207144794775 28.395367077824595 30.981439588752053 20.111121885475598 48-49 20.10302065445672 29.1430738770896 30.416071860374057 20.337833608079627 50-51 18.917049531662926 30.91073793622367 30.32198710922301 19.850225422890396 52-53 18.85512421273833 30.131485434354865 31.087676186431416 19.925714166475387 54-55 19.122403463399007 29.043711051183823 31.606952820148987 20.226932665268183 56-57 19.725147325795916 29.467614101542793 29.319152905825646 21.488085666835648 58-59 20.11903899760768 30.056917285203838 28.902553237976115 20.921490479212366 60-61 19.418037022625757 28.269122894447097 31.907925827092726 20.40491425583442 62-63 19.956216528720706 28.262003630824445 31.510720015318693 20.271059825136156 64-65 20.297695690758825 29.481607136939036 29.843216630599823 20.377480541702315 66-67 20.159913390475655 30.845498477336807 28.875671880504388 20.118916251683153 68-69 20.983047560363378 28.469260124390722 31.2144727264693 19.333219588776604 70-71 21.87639239908137 28.390089003069875 30.28381312669466 19.449705471154093 72-73 20.667590534325285 29.58520469724104 30.052375685996285 19.69482908243739 74-75 19.518627307776814 30.303084236845628 31.269401527695777 18.908886927681785 76-77 20.0599982079095 28.428876715220863 31.720431427375534 19.790693649494102 78-79 19.470326786474875 28.582309120881416 31.05944462378988 20.887919468853834 80-81 20.17605447955114 29.36150024978796 30.695564330525315 19.766880940135582 82-83 19.736808188626117 29.311419912580348 31.78020869262088 19.17156320617265 84-85 19.527035403607012 28.979330813768655 31.723193210677426 19.77044057194691 86-87 18.295525542813166 30.81794201728017 29.831371648882826 21.055160791023837 88-89 19.683475084418507 29.062122939863087 30.690899985393234 20.563501990325168 90-91 19.80624413116726 29.215279525215266 31.422512995820473 19.555963347797 92-93 18.878936922096845 30.418772270713685 31.39540021922422 19.306890587965253 94-95 18.784622277321912 30.356763657119007 30.9223810429798 19.936233022579277 96-97 20.255532465683306 29.380219003278548 30.41920188144953 19.945046649588615 98-99 20.31957332940541 28.893369175627242 31.392387194972258 19.39467029999509 100-101 19.613104370987738 29.124390872601847 31.439995581141293 19.822509175269122 102-103 18.912750087917036 30.087266482833623 30.889413561134106 20.110569868115235 104-105 19.566737062534482 29.097981596421164 30.696035307179457 20.639246033864893 106-107 19.674408127766945 28.438100534039908 30.482317638661026 21.405173699532124 108-109 18.957111241222112 29.750861985371102 30.11271785920762 21.17930891419916 110-111 18.65392747696408 29.93194022229137 30.85963752598504 20.55449477475951 112-113 19.351553998191807 28.646143100287308 30.297824335015704 21.70447856650518 114-115 19.035525050312987 29.173492190822937 29.17416732697028 22.616815431893798 116-117 19.71202119119456 29.205833470401803 28.187639779152008 22.89450555925163 118-119 19.675987583022277 28.135084344862037 30.031386132901922 22.15754193921376 120-121 19.06284253952745 28.12828730543141 30.860195387921983 21.94867476711915 122-123 19.876504491610568 28.767665559286375 29.818500513388297 21.537329435714764 124-125 20.301528509572748 28.927158739754443 28.936487527349612 21.834825223323197 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21.0 1 12.0 2 22.0 3 28.5 4 13.5 5 13.0 6 30.0 7 68.5 8 115.0 9 153.0 10 172.5 11 193.0 12 252.5 13 330.0 14 438.0 15 580.0 16 702.5 17 858.5 18 1059.5 19 1288.0 20 1558.5 21 1909.0 22 2343.0 23 2785.0 24 3460.5 25 4446.0 26 5542.0 27 6864.0 28 8454.5 29 10425.5 30 13327.5 31 17172.0 32 22259.0 33 29129.5 34 37632.5 35 45761.5 36 51265.0 37 52478.5 38 48267.0 39 41413.5 40 36038.5 41 32471.5 42 29487.5 43 27355.5 44 26068.0 45 24960.5 46 24000.0 47 24348.0 48 26134.5 49 24872.0 50 21476.5 51 18911.5 52 16332.5 53 14238.0 54 12131.0 55 10000.5 56 8161.0 57 6559.0 58 5077.0 59 3865.5 60 2776.0 61 1955.5 62 1447.0 63 1039.5 64 701.0 65 446.5 66 296.0 67 185.5 68 121.5 69 87.5 70 60.0 71 54.5 72 46.0 73 41.0 74 39.0 75 25.0 76 13.5 77 9.5 78 4.5 79 2.0 80 1.5 81 0.5 82 0.0 83 0.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 1.5 98 5.5 99 5.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01755266720756704 2 0.0 3 0.0 4 0.0 5 0.14177154283034918 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.137296226422042E-5 18-19 0.0 20-21 0.00429610735849543 22-23 4.2961073584954295E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 7.364755471706451E-4 92-93 0.0 94-95 7.364755471706451E-4 96-97 0.0 98-99 0.0013502051698128493 100-101 1.2274592452844084E-4 102-103 3.068648113211021E-4 104-105 0.0015343240566055105 106-107 0.006321415113214704 108-109 2.454918490568817E-4 110-111 0.0048484640188734135 112-113 0.008162603981141317 114-115 0.004971209943401854 116-117 0.001595697018869731 118-119 0.0 120-121 4.909836981137634E-4 122-123 6.751025849064246E-4 124-125 0.0010433403584917472 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 814691.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.23167267864834 #Duplication Level Percentage of deduplicated Percentage of total 1 88.24298148578522 68.1515306229819 2 8.65420727867505 13.36757807679615 3 1.697774705186859 3.9336594093924053 4 0.5271533895402227 1.6285175212964191 5 0.24799104692393192 0.9576381681632216 6 0.14289407125844766 0.6621568883491127 7 0.08177037664330294 0.4420684074607754 8 0.0589113687791201 0.3639858840480117 9 0.044788094199462176 0.31131534880019973 >10 0.2559245449628551 3.6931879399670526 >50 0.023677219293187828 1.2398828112610845 >100 0.01938422532261969 3.1533086305856233 >500 0.0014299838042281432 0.7320947613621829 >1k 0.001112209625510778 1.3630755295358914 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1979 0.24291418464178444 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1846 0.2265889766795018 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1805 0.22155639377383574 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1680 0.20621315320778064 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1542 0.1892742156228558 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA 1208 0.14827707683035654 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA 1048 0.128637728905806 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 896 0.109980348377483 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 817 0.10028342033973617 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2274592452844084E-4 0.0 12-13 0.0 0.0 0.0 1.2274592452844084E-4 0.0 14-15 0.0 0.0 0.0 1.2274592452844084E-4 0.0 16-17 0.0 0.0 0.0 1.2274592452844084E-4 0.0 18-19 0.0 0.0 0.0 1.2274592452844084E-4 0.0 20-21 0.0 0.0 0.0 1.2274592452844084E-4 0.0 22-23 0.0 0.0 0.0 2.454918490568817E-4 0.0 24-25 0.0 0.0 0.0 3.6823777358532253E-4 0.0 26-27 0.0 0.0 0.0 7.978485094348655E-4 0.0 28-29 0.0 0.0 0.0 0.001166086283020188 0.0 30-31 0.0 0.0 0.0 0.0027617833018899187 0.0 32-33 0.0 0.0 0.0 0.007978485094348654 0.0 34-35 0.0 0.0 0.0 0.01417715428303492 0.0 36-37 0.0 0.0 0.0 0.025285660452858814 0.0 38-39 0.0 0.0 0.0 0.04609109466042954 0.0 40-41 0.0 0.0 0.0 0.0718063658491379 0.0 42-43 0.0 0.0 0.0 0.09715339926426093 0.0 44-45 0.0 0.0 0.0 0.12538496190580234 0.0 46-47 0.0 0.0 0.0 0.15803537783036758 0.0 48-49 0.0 0.0 0.0 0.1974368196039971 0.0 50-51 0.0 0.0 0.0 0.23898631505687432 0.0 52-53 0.0 0.0 0.0 0.28170189679277174 0.0 54-55 0.0 0.0 0.0 0.3281612292267866 0.0 56-57 0.0 0.0 0.0 0.3813102145476015 0.0 58-59 0.0 0.0 0.0 0.44765438675522373 0.0 60-61 0.0 0.0 0.0 0.5279915943590883 0.0 62-63 0.0 0.0 0.0 0.6088197856610666 0.0 64-65 0.0 0.0 0.0 0.6869475666234193 0.0 66-67 0.0 0.0 0.0 0.7807868259254123 0.0 68-69 0.0 0.0 0.0 0.8961066220198823 0.0 70-71 0.0 0.0 0.0 1.0301451716049397 0.0 72-73 0.0 0.0 0.0 1.1566348468314982 0.0 74-75 0.0 0.0 0.0 1.27692585286937 0.0 76-77 0.0 0.0 0.0 1.411946369850655 0.0 78-79 0.0 0.0 0.0 1.5727435309829128 0.0 80-81 0.0 0.0 0.0 1.7515229700585866 0.0 82-83 0.0 0.0 0.0 1.934291651681435 0.0 84-85 0.0 0.0 0.0 2.1190856410590024 0.0 86-87 0.0 0.0 0.0 2.2981105719837336 0.0 88-89 0.0 0.0 0.0 2.5043237251915142 0.0 90-91 0.0 0.0 0.0 2.7317719233427153 0.0 92-93 0.0 0.0 0.0 2.9724766813429877 0.0 94-95 0.0 0.0 0.0 3.20649178645646 0.0 96-97 0.0 0.0 0.0 3.440875129343518 0.0 98-99 0.0 0.0 0.0 3.706497310023064 0.0 100-101 0.0 0.0 0.0 3.996054945985656 0.0 102-103 0.0 0.0 0.0 4.301753671023738 0.0 104-105 0.0 0.0 0.0 4.60309491574106 0.0 106-107 0.0 0.0 0.0 4.908977759665935 0.0 108-109 0.0 0.0 0.0 5.2325360167229045 0.0 110-111 0.0 0.0 0.0 5.591445100044066 0.0 112-113 0.0 0.0 0.0 6.007308292346424 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGGGCG 105 6.3027983E-9 45.395805 5 TACACCG 70 5.4064585E-5 42.55857 5 TACCTGG 845 0.0 42.306744 2 GTACATG 5580 0.0 35.563553 1 GTACCTG 1035 0.0 35.122437 1 TACATGG 5560 0.0 34.93477 2 CATGGGG 2585 0.0 33.88222 4 ACCTGGG 1040 0.0 33.801323 3 ACATGGG 5350 0.0 33.076363 3 GTATCAA 6080 0.0 32.442894 1 ACCGCAT 75 0.0039116796 31.730259 8 TAGGACC 1485 0.0 30.894367 4 ATGCCGG 285 0.0 29.225235 9 TAGCCCT 885 0.0 28.949442 4 AATGCCG 290 0.0 28.721352 8 ATCAACG 6920 0.0 28.41338 3 TCAACGC 6985 0.0 28.148975 4 GTGACGA 85 0.00721257 27.997286 6 CAACGCA 7045 0.0 27.909239 5 TATCAAC 7055 0.0 27.869677 2 >>END_MODULE