FastQCFastQC Report
Fri 27 May 2016
SRR939977_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939977_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400108
Sequences flagged as poor quality0
Sequence length125
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT24250.6060863566836954No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14560.3639017465284373No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9090.22718865906205324No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6580.164455596988813No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT6470.16170633928839212No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5120.12796544932868126No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC4440.11097003808971577No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4270.1067211852799744No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA4210.10522159017065393No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4120.10297219750667319No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA4100.10247233247023303No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCG100.00126166762538.3518108-109
CGACGCC304.404428E-61692.2346108-109
CCGTTCG150.00283838481692.2346108-109
CGCGGAT200.0035301051516.2722110-111
CGTTGTT200.0035301051516.2722110-111
CCGCTCC1650.01384.5555108-109
CTCCGCT250.00551519361213.0177110-111
CGTGACG250.00551519361213.0177110-111
AACGGCG250.0078823621015.3408108-109
CGCTAGT300.0079410221010.84814110-111
CGGCTCG300.0079410221010.84814110-111
CGCCGCC1201.200533E-101010.84814110-111
CCGCGCC1350.0940.1303108-109
GGTCGCC552.7120655E-5923.037108-109
CCGCTCG552.7120655E-5923.037108-109
CGTGTCT703.2643264E-5866.4412110-111
CCGTGCC654.475347E-5781.0313108-109
CGTGGCC756.872979E-5676.8938108-109
CGTCGCC756.872979E-5676.8938108-109
CCTCTCG808.3400446E-5634.58795108-109
Produced by FastQC (version 0.11.5)