##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939977_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 400108 Sequences flagged as poor quality 0 Sequence length 125 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.560346206524237 33.0 31.0 34.0 29.0 34.0 2 31.565442330570747 34.0 31.0 34.0 28.0 34.0 3 31.75822277984944 34.0 31.0 34.0 29.0 34.0 4 34.97603647015306 37.0 35.0 37.0 32.0 37.0 5 35.04307087086487 37.0 35.0 37.0 32.0 37.0 6 35.06825657072591 37.0 35.0 37.0 32.0 37.0 7 35.00997730612735 37.0 36.0 37.0 32.0 37.0 8 35.03095914103192 37.0 36.0 37.0 32.0 37.0 9 36.61241964669539 39.0 38.0 39.0 33.0 39.0 10-11 36.582151569076345 39.0 38.0 39.0 33.0 39.0 12-13 36.508388984974054 39.0 38.0 39.0 32.0 39.0 14-15 37.987478380837175 41.0 39.0 41.0 33.0 41.0 16-17 37.99988628070421 41.0 39.0 41.0 33.0 41.0 18-19 38.019114838993474 41.0 39.0 41.0 33.0 41.0 20-21 38.09166025173203 41.0 39.0 41.0 33.0 41.0 22-23 38.079307337019 41.0 39.0 41.0 33.0 41.0 24-25 38.10135513411379 41.0 39.0 41.0 33.0 41.0 26-27 38.0747860577644 41.0 39.0 41.0 33.0 41.0 28-29 38.02152918711948 41.0 39.0 41.0 33.0 41.0 30-31 37.94041733731892 40.0 38.0 41.0 33.0 41.0 32-33 37.8412153718496 40.0 38.0 41.0 33.0 41.0 34-35 37.740605036640105 40.0 38.0 41.0 32.0 41.0 36-37 37.89830495766143 41.0 38.0 41.0 33.0 41.0 38-39 37.85492542013657 41.0 38.0 41.0 33.0 41.0 40-41 37.78903446069562 41.0 38.0 41.0 32.0 41.0 42-43 37.69577838983474 40.5 38.0 41.0 32.0 41.0 44-45 37.57168689453847 40.0 38.0 41.0 32.0 41.0 46-47 37.44443250322413 40.0 38.0 41.0 32.0 41.0 48-49 37.32917487278435 40.0 38.0 41.0 31.0 41.0 50-51 37.1871907085087 40.0 37.5 41.0 31.0 41.0 52-53 37.07425120217542 40.0 37.0 41.0 31.0 41.0 54-55 36.89547947054295 40.0 37.0 41.0 30.0 41.0 56-57 36.649438401631556 40.0 36.0 41.0 30.0 41.0 58-59 36.45833499955012 40.0 36.0 41.0 30.0 41.0 60-61 36.216858948084024 39.5 35.5 41.0 30.0 41.0 62-63 35.972848580883166 39.0 35.0 41.0 29.0 41.0 64-65 35.67395303268118 39.0 35.0 41.0 29.0 41.0 66-67 35.333938586581624 38.5 35.0 41.0 28.5 41.0 68-69 34.91172758355244 37.5 35.0 40.0 28.0 41.0 70-71 34.484159276995214 37.0 34.0 40.0 27.5 41.0 72-73 34.114340378097914 36.5 34.0 39.0 27.0 41.0 74-75 33.70940346106551 36.0 34.0 39.0 26.0 41.0 76-77 33.259359972807346 35.5 34.0 38.5 26.0 40.0 78-79 32.780260579643496 35.0 33.5 37.0 25.5 39.0 80-81 32.295797634638646 35.0 33.0 37.0 23.0 39.0 82-83 32.09868710448178 35.0 33.0 36.5 23.0 39.0 84-85 31.684392713967227 35.0 33.0 36.0 16.5 37.5 86-87 31.019869635198496 35.0 34.0 36.0 2.0 37.0 88-89 15.791533785877812 18.0 16.5 18.5 2.0 19.0 90-91 2.719228308356744 2.0 2.0 2.0 2.0 2.0 92-93 2.2738885500914754 2.0 2.0 2.0 2.0 2.0 94-95 2.0930786187729313 2.0 2.0 2.0 2.0 2.0 96-97 2.0 2.0 2.0 2.0 2.0 2.0 98-99 2.0 2.0 2.0 2.0 2.0 2.0 100-101 2.0 2.0 2.0 2.0 2.0 2.0 102-103 2.0 2.0 2.0 2.0 2.0 2.0 104-105 2.0 2.0 2.0 2.0 2.0 2.0 106-107 2.0 2.0 2.0 2.0 2.0 2.0 108-109 2.0 2.0 2.0 2.0 2.0 2.0 110-111 2.0 2.0 2.0 2.0 2.0 2.0 112-113 2.0 2.0 2.0 2.0 2.0 2.0 114-115 2.0 2.0 2.0 2.0 2.0 2.0 116-117 2.0 2.0 2.0 2.0 2.0 2.0 118-119 2.0 2.0 2.0 2.0 2.0 2.0 120-121 2.0 2.0 2.0 2.0 2.0 2.0 122-123 2.0 2.0 2.0 2.0 2.0 2.0 124-125 2.0 2.0 2.0 2.0 2.0 2.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.15657785211885766 1101 2 -0.07301290246995151 1101 3 -0.0868531693448702 1101 4 -0.12180081678106802 1101 5 -0.06075398872779658 1101 6 -0.24277061287647683 1101 7 -0.3163847895032674 1101 8 -0.1792407423155069 1101 9 -0.07076977718070054 1101 10-11 -0.07882102961207238 1101 12-13 -0.17247783863689392 1101 14-15 -0.2708155463802484 1101 16-17 -0.30733938538600114 1101 18-19 -0.1965169768219326 1101 20-21 -0.21824191445144692 1101 22-23 -0.11661713180031086 1101 24-25 -0.10428792960567534 1101 26-27 -0.03553430128311419 1101 28-29 -0.08938364370809637 1101 30-31 -0.1127670352895862 1101 32-33 -0.036926692948149764 1101 34-35 0.12413868716961218 1101 36-37 0.07471976020445936 1101 38-39 -0.021232529668260725 1101 40-41 -0.003735568724486882 1101 42-43 -0.04343256849526256 1101 44-45 0.07307571719866246 1101 46-47 -0.055917146491353265 1101 48-49 -0.016701614548324528 1101 50-51 -0.03284060397709254 1101 52-53 -0.1133517895096503 1101 54-55 -0.04837270835388097 1101 56-57 0.10765256014887825 1101 58-59 -0.1255944927819499 1101 60-61 -0.03590837263017477 1101 62-63 -0.25520584960110426 1101 64-65 -0.16574860963718407 1101 66-67 -0.2194318198509606 1101 68-69 -0.18207776237057516 1101 70-71 -0.05855036804641145 1101 72-73 -0.2443303824405021 1101 74-75 -0.09678451542271915 1101 76-77 -0.19683017007817227 1101 78-79 -0.2335763777225921 1101 80-81 -0.18084412055110732 1101 82-83 -6.296453997833851E-4 1101 84-85 0.09591864375543224 1101 86-87 0.080917367066629 1101 88-89 0.4737489414344047 1101 90-91 0.2325738631169747 1101 92-93 -0.07091722247750365 1101 94-95 0.05608805859673982 1101 96-97 0.0 1101 98-99 0.0 1101 100-101 0.0 1101 102-103 0.0 1101 104-105 0.0 1101 106-107 0.0 1101 108-109 0.0 1101 110-111 0.0 1101 112-113 0.0 1101 114-115 0.0 1101 116-117 0.0 1101 118-119 0.0 1101 120-121 0.0 1101 122-123 0.0 1101 124-125 0.0 1102 1 -0.14428434462750062 1102 2 0.02044358672686286 1102 3 -0.07062520956371543 1102 4 -0.19189566755007093 1102 5 -0.020916003379319648 1102 6 -0.22191271681489866 1102 7 -0.12246729377552867 1102 8 0.03205060586365249 1102 9 -0.22622038457004834 1102 10-11 -0.1279967154917614 1102 12-13 -0.24516088339957776 1102 14-15 -0.30105088491535525 1102 16-17 -0.44185095691487675 1102 18-19 -0.33088117198136047 1102 20-21 -0.3673983917256791 1102 22-23 -0.24984205794653747 1102 24-25 -0.24316614704709139 1102 26-27 -0.3625757123870059 1102 28-29 -0.4148519362617833 1102 30-31 -0.20910315939043045 1102 32-33 -0.3881096780979121 1102 34-35 -0.37891978647486013 1102 36-37 -0.2946137011862362 1102 38-39 -0.1655984469785281 1102 40-41 -0.1542002182667872 1102 42-43 -0.3083573237511317 1102 44-45 -0.32161807113575946 1102 46-47 -0.44839697745563 1102 48-49 -0.594844208637376 1102 50-51 -0.3089527159963765 1102 52-53 -0.31221550114211283 1102 54-55 -0.2729660158107947 1102 56-57 -0.3069817477451977 1102 58-59 -0.24579730916092757 1102 60-61 -0.28761893206424816 1102 62-63 -0.3538221676426119 1102 64-65 -0.3130421833651056 1102 66-67 -0.4425894496415239 1102 68-69 -0.2906709638489744 1102 70-71 -0.26810962990607123 1102 72-73 -0.1876467812906384 1102 74-75 -0.3644912066410768 1102 76-77 -0.38172127715621684 1102 78-79 -0.6497705568527721 1102 80-81 -0.5857184697768005 1102 82-83 -0.2403199231959654 1102 84-85 -0.21278848462429067 1102 86-87 0.0018143427048187277 1102 88-89 0.7077561214786492 1102 90-91 -0.10576750692025039 1102 92-93 -0.15203880632637823 1102 94-95 0.007109426514394723 1102 96-97 0.0 1102 98-99 0.0 1102 100-101 0.0 1102 102-103 0.0 1102 104-105 0.0 1102 106-107 0.0 1102 108-109 0.0 1102 110-111 0.0 1102 112-113 0.0 1102 114-115 0.0 1102 116-117 0.0 1102 118-119 0.0 1102 120-121 0.0 1102 122-123 0.0 1102 124-125 0.0 1103 1 0.3239363801949331 1103 2 0.27464324886993197 1103 3 0.4069462396415702 1103 4 0.2151842563379205 1103 5 0.4314731726391159 1103 6 0.2917051782987343 1103 7 0.3418944517767599 1103 8 0.3263221097037885 1103 9 0.4611803150073186 1103 10-11 0.5077567745452285 1103 12-13 0.37339832634175707 1103 14-15 0.5066987869615076 1103 16-17 0.49169390332664875 1103 18-19 0.31499389631220964 1103 20-21 0.44810377796893164 1103 22-23 0.5000449044182176 1103 24-25 0.40744394218984326 1103 26-27 0.5405934233146681 1103 28-29 0.600356335581175 1103 30-31 0.5259664529532841 1103 32-33 0.6429725316622594 1103 34-35 0.43754320311939665 1103 36-37 0.6559872822411847 1103 38-39 0.546317289744259 1103 40-41 0.6189976164345055 1103 42-43 0.5781324808821537 1103 44-45 0.5397491190626198 1103 46-47 0.5373979597142835 1103 48-49 0.676956355294827 1103 50-51 0.38865120012970067 1103 52-53 0.3187605788382939 1103 54-55 0.4464211946087602 1103 56-57 0.48544661465346195 1103 58-59 0.687870008518793 1103 60-61 0.6130099967785583 1103 62-63 0.4630019143975872 1103 64-65 0.6472486373509199 1103 66-67 0.6722662075781329 1103 68-69 0.44275175294779245 1103 70-71 0.6669314090353993 1103 72-73 0.6584838776559749 1103 74-75 0.5856309368036818 1103 76-77 0.6202113524131789 1103 78-79 0.5099704613651213 1103 80-81 0.41369279253959235 1103 82-83 0.6798315472352741 1103 84-85 0.6435375410352577 1103 86-87 0.5837587784272245 1103 88-89 0.6387654847627715 1103 90-91 -0.5325834879299278 1103 92-93 -0.3035814560541459 1103 94-95 -0.10632070052734788 1103 96-97 0.0 1103 98-99 0.0 1103 100-101 0.0 1103 102-103 0.0 1103 104-105 0.0 1103 106-107 0.0 1103 108-109 0.0 1103 110-111 0.0 1103 112-113 0.0 1103 114-115 0.0 1103 116-117 0.0 1103 118-119 0.0 1103 120-121 0.0 1103 122-123 0.0 1103 124-125 0.0 1104 1 -0.0592920711978131 1104 2 0.019290086574841325 1104 3 -0.014780756551605378 1104 4 0.16991470078635018 1104 5 0.12834942390158233 1104 6 -0.08371838338439375 1104 7 -0.09398712426115452 1104 8 -0.01973152052611482 1104 9 -0.043280902579382996 1104 10-11 -0.06239609611232311 1104 12-13 0.0808329917653765 1104 14-15 -0.003659425204794786 1104 16-17 0.08149645037411801 1104 18-19 -0.020682099169796686 1104 20-21 0.11639148317596693 1104 22-23 -0.15554342051858328 1104 24-25 0.035217636245334916 1104 26-27 0.06193585239237365 1104 28-29 -0.03631738297665521 1104 30-31 -0.1381080460935138 1104 32-33 -0.12216363557512722 1104 34-35 0.052036333501696674 1104 36-37 0.05567263641943043 1104 38-39 -0.004487701560400126 1104 40-41 -0.09589830840906899 1104 42-43 -0.3515383511812402 1104 44-45 -0.15994938270931414 1104 46-47 -0.3149545631296604 1104 48-49 -0.33521263909354104 1104 50-51 -0.26097588630378254 1104 52-53 -0.17252914034105515 1104 54-55 -0.06119843355337906 1104 56-57 -0.23855850095571896 1104 58-59 -0.2261611632306213 1104 60-61 -0.1311692049234594 1104 62-63 -0.21229584871863239 1104 64-65 -0.5191864523814047 1104 66-67 -0.3917147642680874 1104 68-69 -0.28246951507785667 1104 70-71 -0.24967400179703247 1104 72-73 -0.4120260696938871 1104 74-75 -0.4685162325791481 1104 76-77 -0.36500806299699207 1104 78-79 -0.387009177793189 1104 80-81 -0.2845624620157494 1104 82-83 -0.3233985774875805 1104 84-85 -0.13956003203847445 1104 86-87 0.04369298069697436 1104 88-89 0.6775927194054852 1104 90-91 -0.5640346237027374 1104 92-93 -0.3035814560541459 1104 94-95 -0.10632070052734788 1104 96-97 0.0 1104 98-99 0.0 1104 100-101 0.0 1104 102-103 0.0 1104 104-105 0.0 1104 106-107 0.0 1104 108-109 0.0 1104 110-111 0.0 1104 112-113 0.0 1104 114-115 0.0 1104 116-117 0.0 1104 118-119 0.0 1104 120-121 0.0 1104 122-123 0.0 1104 124-125 0.0 1105 1 -0.13776280517678074 1105 2 -0.18565796431629877 1105 3 -0.10457268884143645 1105 4 -0.20705769248965566 1105 5 -0.1888701910691637 1105 6 -0.2635283102306474 1105 7 -0.22027454886939069 1105 8 -0.4226739469880556 1105 9 -0.22919451062358576 1105 10-11 -0.3184342192529641 1105 12-13 -0.34391992217723555 1105 14-15 -0.31095038542687803 1105 16-17 -0.35294549231457495 1105 18-19 -0.30144239178479637 1105 20-21 -0.24944056160244088 1105 22-23 -0.4817557924543152 1105 24-25 -0.29396067262806014 1105 26-27 -0.15187836059445914 1105 28-29 -0.26758360294428485 1105 30-31 -0.2829416190528562 1105 32-33 -0.3691230980894815 1105 34-35 -0.27006170518207995 1105 36-37 -0.4183392454132999 1105 38-39 -0.3584288711161463 1105 40-41 -0.15565560795965894 1105 42-43 -0.22047649150261606 1105 44-45 -0.5080219758243629 1105 46-47 -0.5488837965977282 1105 48-49 -0.5076348510675999 1105 50-51 -0.24543184485300173 1105 52-53 -0.16416483647719815 1105 54-55 -0.3510328655493282 1105 56-57 -0.38192719042754675 1105 58-59 -0.4620074047193725 1105 60-61 -0.4142172476763548 1105 62-63 -0.44365249359289294 1105 64-65 -0.6008672705459546 1105 66-67 -0.6301335943506459 1105 68-69 -0.5537306712507117 1105 70-71 -0.46819483117427296 1105 72-73 -0.6545592330284009 1105 74-75 -0.4741316192812661 1105 76-77 -0.43875861684955453 1105 78-79 -0.5628810999554261 1105 80-81 -0.6928535217052314 1105 82-83 -0.4897125870183672 1105 84-85 -0.37554191088718625 1105 86-87 -0.47559465188854233 1105 88-89 0.2933818123532461 1105 90-91 -0.742791100386675 1105 92-93 -0.3035814560541459 1105 94-95 -0.10632070052734788 1105 96-97 0.0 1105 98-99 0.0 1105 100-101 0.0 1105 102-103 0.0 1105 104-105 0.0 1105 106-107 0.0 1105 108-109 0.0 1105 110-111 0.0 1105 112-113 0.0 1105 114-115 0.0 1105 116-117 0.0 1105 118-119 0.0 1105 120-121 0.0 1105 122-123 0.0 1105 124-125 0.0 1106 1 -0.5174737283432016 1106 2 -0.5816753268258061 1106 3 -0.3764033596249874 1106 4 -0.4933243054578398 1106 5 -0.14726281647750028 1106 6 -0.34520370290080393 1106 7 -0.34293978357651156 1106 8 -0.07975543443603783 1106 9 -0.04921509504207222 1106 10-11 -0.37998610541218625 1106 12-13 -0.3065457779968028 1106 14-15 -0.43600520262829434 1106 16-17 -0.4818621254196884 1106 18-19 -0.3456809920443433 1106 20-21 -0.4064773003145703 1106 22-23 -0.4462789946738468 1106 24-25 -0.47568976378123295 1106 26-27 -0.5149606535196867 1106 28-29 -0.4140685897434082 1106 30-31 -0.22701464898975843 1106 32-33 -0.29052641844568683 1106 34-35 -0.41879755339436997 1106 36-37 -0.37320119556321174 1106 38-39 -0.4313111371921252 1106 40-41 -0.41941808166977523 1106 42-43 -0.8055527880972377 1106 44-45 -0.5070509282564473 1106 46-47 -0.4661657583823029 1106 48-49 -0.34495977163859237 1106 50-51 -0.395004494425649 1106 52-53 -0.7055081935273648 1106 54-55 -0.44910419503426624 1106 56-57 -0.5528315623790405 1106 58-59 -0.6447254429347922 1106 60-61 -0.5393973613381391 1106 62-63 -0.47286446835460794 1106 64-65 -0.3913178903159533 1106 66-67 -0.42998144864876053 1106 68-69 -0.5184820920230777 1106 70-71 -0.33136214669760733 1106 72-73 -0.11549358663290832 1106 74-75 -0.18011005039058858 1106 76-77 -0.12952247777047887 1106 78-79 -0.06602693608555654 1106 80-81 -0.1308004497045161 1106 82-83 -0.11177818866512013 1106 84-85 -0.16653846523452387 1106 86-87 -0.19467998772780604 1106 88-89 0.7923839155438301 1106 90-91 -0.1709962285918034 1106 92-93 -0.2787951312678212 1106 94-95 -0.10632070052734788 1106 96-97 0.0 1106 98-99 0.0 1106 100-101 0.0 1106 102-103 0.0 1106 104-105 0.0 1106 106-107 0.0 1106 108-109 0.0 1106 110-111 0.0 1106 112-113 0.0 1106 114-115 0.0 1106 116-117 0.0 1106 118-119 0.0 1106 120-121 0.0 1106 122-123 0.0 1106 124-125 0.0 1107 1 0.09482562541956696 1107 2 -0.010610077711778132 1107 3 0.09693414298081038 1107 4 0.4382579334414771 1107 5 0.32770070238846216 1107 6 0.19788572799350135 1107 7 0.22012046241056282 1107 8 0.14501004398798045 1107 9 0.33748492580429 1107 10-11 0.42989507032265806 1107 12-13 0.4130289562120737 1107 14-15 0.4353102028334561 1107 16-17 0.45484831860783004 1107 18-19 0.5569664959690002 1107 20-21 0.40944932049009 1107 22-23 0.37375435950583835 1107 24-25 0.5339412556058818 1107 26-27 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-0.022961332558146808 2216 36-37 0.1096181622561545 2216 38-39 0.005903000022016158 2216 40-41 -0.055742867994560186 2216 42-43 0.153450445905996 2216 44-45 0.1339111877056638 2216 46-47 -0.014577631794182366 2216 48-49 0.17637887470004188 2216 50-51 0.13835193893078213 2216 52-53 0.11606722804805258 2216 54-55 0.019791623861550534 2216 56-57 -0.011488296035771839 2216 58-59 0.3016594034500528 2216 60-61 0.23865761671683572 2216 62-63 0.08586734037719879 2216 64-65 -0.013262912260977089 2216 66-67 -0.045013788681103506 2216 68-69 0.15179972825874444 2216 70-71 -0.1543582196936839 2216 72-73 -0.3289378000771208 2216 74-75 -0.34395851423904844 2216 76-77 -0.32655989980790423 2216 78-79 -0.28448385454247926 2216 80-81 -0.37609959500365875 2216 82-83 -0.5073025591894904 2216 84-85 -0.4334707821668431 2216 86-87 -0.5028516208994382 2216 88-89 -1.214049440889525 2216 90-91 -0.742791100386675 2216 92-93 -0.3035814560541459 2216 94-95 -0.10632070052734788 2216 96-97 0.0 2216 98-99 0.0 2216 100-101 0.0 2216 102-103 0.0 2216 104-105 0.0 2216 106-107 0.0 2216 108-109 0.0 2216 110-111 0.0 2216 112-113 0.0 2216 114-115 0.0 2216 116-117 0.0 2216 118-119 0.0 2216 120-121 0.0 2216 122-123 0.0 2216 124-125 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6522.0 3 1091.0 4 707.0 5 678.0 6 681.0 7 653.0 8 666.0 9 782.0 10 977.0 11 1097.0 12 1381.0 13 1580.0 14 1746.0 15 2057.0 16 2298.0 17 2666.0 18 3252.0 19 4085.0 20 4446.0 21 6200.0 22 7302.0 23 8974.0 24 13830.0 25 23661.0 26 44398.0 27 112847.0 28 140529.0 29 4811.0 30 191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.996390974436906 19.515480820178553 14.231907385006048 23.2562208203785 2 13.716080948346319 20.626232482810845 43.1824486949741 22.47523787386874 3 19.949143261482146 26.168217288790764 28.462271250940752 25.420368198786342 4 11.656819085387657 17.28831867656509 39.04468043077646 32.01018180727079 5 12.596176687546103 38.811997549479266 33.80843429228711 14.783391470687521 6 29.456546732382254 36.68734441700741 19.73167244843892 14.124436402171414 7 28.28636268207584 32.094334529676985 21.952322872824336 17.666979915422836 8 25.02449338678557 36.91853199636098 20.16280604236856 17.89416857448489 9 25.944744918871905 19.098093514751017 21.74712827536565 33.21003329101143 10-11 24.44867666153052 26.89115745722066 28.714051668872337 19.946114212376486 12-13 26.574889496237812 24.43742119147862 28.261344990433628 20.72634432184994 14-15 21.615565816686765 28.32026392906906 25.998013033997115 24.06615722024706 16-17 20.94542328105142 30.164593676933514 28.338704819841716 20.551278222173348 18-19 21.614910092531954 29.022590594834007 28.95160443713476 20.410894875499277 20-21 22.786292358372197 28.205964538688022 27.27781693485096 21.729926168088816 22-23 21.592136044628397 29.481959690480476 27.60069881830724 21.32520544658389 24-25 22.336594119587712 28.438321653153647 28.623521649154725 20.601562578103913 26-27 20.787387405400544 29.244229058154296 28.895198296459956 21.073185239985204 28-29 20.426038697390307 30.143250075917667 27.338607788715198 22.092103437976828 30-31 21.262634088796027 28.41970168054625 28.175767542763452 22.14189668789427 32-33 20.77657697287192 29.06896241458428 28.941244982529458 21.213215630014346 34-35 21.580048386935527 29.104016915432833 28.62077239145431 20.69516230617733 36-37 20.977237367457498 28.63655392613235 28.728279274944857 21.657929431465295 38-39 21.379352574804802 29.62537614843992 27.999315184900077 20.9959560918552 40-41 22.588276165435335 28.191013426374877 28.637892768952383 20.582817639237405 42-43 20.80738200685815 29.605006648204984 28.25412138722545 21.333489957711418 44-45 20.793312788828988 29.38001584576126 27.93815154446186 21.888519820947895 46-47 22.17413798274466 28.711497895568193 27.70564447599148 21.408719645695662 48-49 21.50939812274982 28.79745767689353 27.877827528420557 21.815316671936095 50-51 20.228438607124335 29.832232587492115 28.598689102303755 21.3406397030798 52-53 20.730527757505474 29.422680876163437 28.31497995541204 21.53181141091905 54-55 20.60546984835136 28.454833448928714 28.724139439268686 22.21555726345124 56-57 21.5971687644336 28.670508962580104 27.356988613074467 22.375333659911824 58-59 22.05292071140792 28.971177781998858 26.896612914513078 22.079288592080136 60-61 20.981335039539324 28.175642576504345 29.547147270237033 21.295875113719294 62-63 21.15466324092495 28.213132454237353 29.048531896387974 21.58367240844972 64-65 21.268132604196865 29.380817179361575 27.499450148459914 21.851600067981643 66-67 21.345736651104204 29.834194767412797 26.74915272876323 22.070915852719768 68-69 21.798989272896318 28.185140012196708 28.592155118118107 21.423715596788867 70-71 22.667004908674656 28.07167064892479 27.94370519959611 21.31761924280444 72-73 21.77099683085567 28.68238075719556 28.15852219900627 21.388100212942508 74-75 20.85474421906085 29.932793145850624 28.394708428724247 20.81775420636428 76-77 21.443335299469144 28.331350535355455 28.679381566976915 21.545932598198487 78-79 21.355260773667077 28.297645007904126 28.450728866617098 21.8963653518117 80-81 21.943200335909303 28.734866586021774 28.113034480690214 21.20889859737871 82-83 21.467543438705935 28.5615739907624 29.176789034850938 20.794093535680723 84-85 20.976461355434033 28.90407090085677 29.005793435772343 21.113674307936858 86-87 20.38080051586523 30.103135555836037 27.653025647049706 21.863038281249022 88-89 30.258125380495986 18.60801593706964 33.94372764465044 17.190131037783928 90-91 30.23009700682553 14.591154074743853 16.181293887164585 38.99745503126603 92-93 22.4272114879556 17.135042491791477 34.86377723879738 25.57396878145554 94-95 15.087472459860388 6.738116458032673 26.791635940843555 51.38277514126338 96-97 26.255467112516506 7.074231575376489 14.537127903173044 52.13317340893396 98-99 57.56960296205762 14.202778726387116 18.49129176627065 9.736326545284612 100-101 30.82630080248511 6.974372249546984 12.409785141082061 49.789541806885836 102-103 22.895184196362433 2.0018331707992947 36.65142576990509 38.45155686293318 104-105 42.0078307288946 4.47655010124227 39.68995487629538 13.825664293567758 106-107 5.179728531478071 1.757042493690744 6.03369483664143 87.02953413818976 108-109 17.707908690533916 10.291124375462191 20.4949958304547 51.505971103549186 110-111 39.89191653098057 2.6390517236069164 24.37750369073016 33.09152805468235 112-113 27.24841076815482 2.7390110750409704 38.14286907234789 31.86970908445632 114-115 27.57506867481292 3.7889551956048124 22.971488112153075 45.66448801742919 116-117 18.8196887484348 4.0400840736986465 19.58999165225687 57.550235525609686 118-119 19.450988061479382 4.159814388450837 14.82181113491506 61.56738641515472 120-121 24.71118660953196 4.099904846445153 29.230661157552575 41.95824738647032 122-123 33.65999523222189 1.5842497486551759 33.19824628683368 31.557508732289257 124-125 38.01018814847016 1.8807352055742514 19.032200844931783 41.07687580102381 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21.0 1 41.5 2 93.5 3 141.0 4 167.5 5 177.0 6 159.5 7 132.0 8 99.0 9 79.0 10 80.5 11 83.0 12 81.0 13 81.5 14 85.0 15 87.0 16 111.0 17 156.5 18 199.5 19 259.5 20 367.5 21 529.5 22 740.5 23 1031.0 24 1440.5 25 1906.5 26 2482.0 27 3250.5 28 4166.0 29 5248.0 30 6706.5 31 8365.5 32 10345.0 33 12563.0 34 14589.0 35 16139.5 36 17090.0 37 17255.0 38 16757.0 39 16391.0 40 15993.5 41 15283.0 42 14828.0 43 15039.5 44 15307.5 45 14958.0 46 14212.5 47 13469.5 48 13066.5 49 12900.5 50 12570.5 51 11817.5 52 10690.0 53 9415.5 54 8320.5 55 7366.5 56 6370.0 57 5557.0 58 4767.5 59 3949.5 60 3154.0 61 2567.5 62 2102.0 63 1628.0 64 1242.0 65 920.5 66 704.5 67 562.5 68 427.0 69 294.5 70 213.0 71 165.5 72 126.5 73 91.0 74 77.0 75 69.5 76 51.0 77 33.0 78 17.0 79 11.5 80 11.0 81 8.5 82 8.0 83 7.5 84 5.5 85 6.5 86 4.0 87 2.5 88 4.0 89 2.0 90 2.0 91 2.5 92 2.0 93 3.0 94 2.5 95 1.5 96 1.5 97 1.0 98 2.0 99 1.5 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 0.0 2 2.499325182200806E-4 3 0.04023913543343297 4 0.04473792076139443 5 0.04823697601647555 6 0.0 7 0.0 8 0.0 9 0.0 10-11 6.248312955502015E-4 12-13 0.0021244264048706847 14-15 1.249662591100403E-4 16-17 3.7489877733012087E-4 18-19 0.0074979755466024174 20-21 0.002499325182200806 22-23 9.997300728803223E-4 24-25 0.0 26-27 0.0 28-29 8.74763813770282E-4 30-31 0.0 32-33 0.0014995951093204835 34-35 0.0 36-37 1.249662591100403E-4 38-39 0.0 40-41 0.0 42-43 0.0 44-45 2.499325182200806E-4 46-47 0.0 48-49 3.7489877733012087E-4 50-51 1.249662591100403E-4 52-53 0.0 54-55 0.0013746288502104432 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.249662591100403E-4 80-81 0.0 82-83 9.997300728803223E-4 84-85 0.0 86-87 8.74763813770282E-4 88-89 19.329406060363702 90-91 26.197801593569736 92-93 40.28399832045348 94-95 44.75466624011517 96-97 48.427049696581925 98-99 93.04725224189468 100-101 51.72553410579144 102-103 53.09941315844722 104-105 74.88140702010458 106-107 81.67869675187698 108-109 12.633963829765962 110-111 39.13755785937797 112-113 52.112429643996116 114-115 86.80731202575305 116-117 66.46705389544823 118-119 71.88434122786848 120-121 80.4316584522179 122-123 75.88626070960841 124-125 49.97988043228329 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 400108.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.88575358017303 #Duplication Level Percentage of deduplicated Percentage of total 1 90.99425323507634 71.78150237914095 2 6.138139305459502 9.684234893825053 3 1.1772107112924017 2.7859546224885783 4 0.5241680440488805 1.6539756462976503 5 0.29552641815401737 1.1656412099464493 6 0.180128281691041 0.8525733145379673 7 0.12227045256589626 0.6751777753874668 8 0.09851770522240869 0.6217314733967244 9 0.0736866530353091 0.5231544438141992 >10 0.3555533007498635 4.941557722034445 >50 0.02407412381873654 1.3900951747767383 >100 0.014571180168109994 2.273429062778178 >500 0.001267059145053043 0.681179119051804 >1k 6.335295725265215E-4 0.9697931625238634 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2425 0.6060863566836954 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1456 0.3639017465284373 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 909 0.22718865906205324 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 658 0.164455596988813 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 647 0.16170633928839212 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 512 0.12796544932868126 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 444 0.11097003808971577 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 427 0.1067211852799744 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 421 0.10522159017065393 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 412 0.10297219750667319 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 410 0.10247233247023303 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 3.7489877733012087E-4 0.0 26-27 0.0 0.0 0.0 7.497975546602417E-4 0.0 28-29 0.0 0.0 0.0 0.001749527627540564 0.0 30-31 0.0 0.0 0.0 0.004123886550631329 0.0 32-33 0.0 0.0 0.0 0.007997840583042579 0.0 34-35 0.0 0.0 0.0 0.01274655842922411 0.0 36-37 0.0 0.0 0.0 0.019869635198496407 0.0 38-39 0.0 0.0 0.0 0.033740889959710876 0.0 40-41 0.0 0.0 0.0 0.04998650364401612 0.0 42-43 0.0 0.0 0.0 0.06798164495586193 0.0 44-45 0.0 0.0 0.0 0.09122536915032942 0.0 46-47 0.0 0.0 0.0 0.11734331730432784 0.0 48-49 0.0 0.0 0.0 0.1454607256040869 0.0 50-51 0.0 0.0 0.0 0.17670229038159696 0.0 52-53 0.0 0.0 0.0 0.20944345026842753 0.0 54-55 0.0 2.499325182200806E-4 0.0 0.24893278814720027 0.0 56-57 0.0 2.499325182200806E-4 0.0 0.2975446629410059 0.0 58-59 0.0 2.499325182200806E-4 0.0 0.35128015435832327 0.0 60-61 0.0 2.499325182200806E-4 0.0 0.40676517340318114 0.0 62-63 0.0 2.499325182200806E-4 0.0 0.47637137972747357 0.0 64-65 0.0 2.499325182200806E-4 0.0 0.5409789356873644 0.0 66-67 0.0 3.7489877733012087E-4 0.0 0.6148339948213982 0.0 68-69 0.0 4.998650364401612E-4 0.0 0.7161816309596409 0.0 70-71 0.0 4.998650364401612E-4 0.0 0.8270267027902467 0.0 72-73 0.0 4.998650364401612E-4 0.0 0.9353724494386515 0.0 74-75 0.0 4.998650364401612E-4 0.0 1.0367200855768943 0.0 76-77 0.0 4.998650364401612E-4 0.0 1.1503144151079208 0.0 78-79 0.0 4.998650364401612E-4 0.0 1.1798064522578904 0.0 80-81 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 82-83 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 84-85 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 86-87 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 88-89 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 90-91 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 92-93 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 94-95 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 96-97 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 98-99 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 100-101 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 102-103 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 104-105 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 106-107 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 108-109 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 110-111 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 112-113 0.0 4.998650364401612E-4 0.0 1.1806812160716607 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTTCG 10 0.0012616676 2538.3518 108-109 CGACGCC 30 4.404428E-6 1692.2346 108-109 CCGTTCG 15 0.0028383848 1692.2346 108-109 CGCGGAT 20 0.003530105 1516.2722 110-111 CGTTGTT 20 0.003530105 1516.2722 110-111 CCGCTCC 165 0.0 1384.5555 108-109 CTCCGCT 25 0.0055151936 1213.0177 110-111 CGTGACG 25 0.0055151936 1213.0177 110-111 AACGGCG 25 0.007882362 1015.3408 108-109 CGCTAGT 30 0.007941022 1010.84814 110-111 CGGCTCG 30 0.007941022 1010.84814 110-111 CGCCGCC 120 1.200533E-10 1010.84814 110-111 CCGCGCC 135 0.0 940.1303 108-109 GGTCGCC 55 2.7120655E-5 923.037 108-109 CCGCTCG 55 2.7120655E-5 923.037 108-109 CGTGTCT 70 3.2643264E-5 866.4412 110-111 CCGTGCC 65 4.475347E-5 781.0313 108-109 CGTGGCC 75 6.872979E-5 676.8938 108-109 CGTCGCC 75 6.872979E-5 676.8938 108-109 CCTCTCG 80 8.3400446E-5 634.58795 108-109 >>END_MODULE