FastQCFastQC Report
Fri 27 May 2016
SRR939977_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939977_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400108
Sequences flagged as poor quality0
Sequence length125
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30370.7590450578343847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.36365181401021723No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT14000.3499055255081128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10950.27367610745098825No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8570.21419216811460906No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT7620.1904485788837014No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7450.18619972607396001No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.18170094074599857No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5290.1322143021384226No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC5110.12771551681046117No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5110.12771551681046117No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT5060.1264658542193608No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG5010.12521619162826036No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC4210.10522159017065393No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA4010.10022293980625231No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG4900.047.405242
GTACCTG5350.043.434181
GTACATG23200.032.1027951
TACATGG23500.031.681082
ACATGGG23400.030.2892783
ACCTGGG7350.029.1724573
GAGTACT16700.029.03422412-13
GATCTAC1107.6796935E-427.0830881
GATCGCG450.00952658226.44483294-95
TAGGACC4750.026.331984
AGTACTT17700.026.21744512-13
CATGGGG12550.026.102184
GTACTTT19250.024.87907614-15
AGAGTAC28000.024.85975810-11
TCGTTAC600.001288348124.78893352-53
AGGGCGC650.002052934122.88209262-63
GTGTAAG2102.7491678E-622.6982041
GTACAGC1350.002554033722.0346076
CATGACT1903.2729407E-521.9433164
TATACTG2755.433867E-821.6583375
Produced by FastQC (version 0.11.5)