##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939977_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 400108 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.82158317254341 34.0 31.0 34.0 31.0 34.0 2 32.88632819138832 34.0 31.0 34.0 31.0 34.0 3 32.932913113459364 34.0 31.0 34.0 31.0 34.0 4 36.33600427884471 37.0 37.0 37.0 35.0 37.0 5 36.241714737021006 37.0 37.0 37.0 35.0 37.0 6 36.391201875493614 37.0 37.0 37.0 35.0 37.0 7 36.39048956781669 37.0 37.0 37.0 35.0 37.0 8 36.405503014186166 37.0 37.0 37.0 35.0 37.0 9 38.23490407589951 39.0 39.0 39.0 37.0 39.0 10-11 38.22596398972277 39.0 39.0 39.0 37.0 39.0 12-13 38.21427589550821 39.0 39.0 39.0 37.0 39.0 14-15 39.72327096683895 41.0 40.0 41.0 37.0 41.0 16-17 39.759150029492034 41.0 40.0 41.0 37.0 41.0 18-19 39.814142681476 41.0 40.0 41.0 37.5 41.0 20-21 39.83139177422096 41.0 40.0 41.0 37.5 41.0 22-23 39.77010957041598 41.0 40.0 41.0 37.0 41.0 24-25 39.726633808871604 41.0 40.0 41.0 37.0 41.0 26-27 39.618919141831704 41.0 40.0 41.0 37.0 41.0 28-29 39.53425200195947 41.0 40.0 41.0 36.0 41.0 30-31 39.39294140582042 41.0 39.0 41.0 36.0 41.0 32-33 39.21477701020724 41.0 39.0 41.0 35.0 41.0 34-35 39.089198416427564 41.0 39.0 41.0 35.0 41.0 36-37 39.058594179571514 41.0 39.0 41.0 35.0 41.0 38-39 39.09700880762195 41.0 39.0 41.0 35.0 41.0 40-41 39.03181141091905 41.0 39.0 41.0 35.0 41.0 42-43 38.9055130114869 41.0 39.0 41.0 35.0 41.0 44-45 38.72661006528237 41.0 39.0 41.0 35.0 41.0 46-47 38.45873864056705 41.0 39.0 41.0 35.0 41.0 48-49 38.187736811061015 41.0 39.0 41.0 35.0 41.0 50-51 38.00305542503524 41.0 39.0 41.0 34.0 41.0 52-53 37.90141661751328 41.0 38.0 41.0 34.0 41.0 54-55 37.78272116528537 41.0 38.0 41.0 34.0 41.0 56-57 37.614809001569576 40.5 38.0 41.0 33.5 41.0 58-59 37.43362167214852 40.0 37.0 41.0 33.0 41.0 60-61 37.21150414388115 40.0 37.0 41.0 33.0 41.0 62-63 37.00772041548782 40.0 36.0 41.0 33.0 41.0 64-65 36.75313290411589 39.0 36.0 41.0 32.5 41.0 66-67 36.46838103711998 39.0 35.5 41.0 32.0 41.0 68-69 36.11783318504004 38.5 35.0 41.0 32.0 41.0 70-71 35.73229478040929 37.0 35.0 40.0 31.0 41.0 72-73 35.383926339888234 37.0 35.0 39.5 31.0 41.0 74-75 35.03979050656323 36.5 35.0 39.0 31.0 41.0 76-77 34.671132544213066 36.0 35.0 39.0 31.0 40.5 78-79 34.30708458716147 35.5 35.0 37.5 30.0 39.5 80-81 34.021213022483934 35.0 35.0 37.0 30.0 39.0 82-83 33.783214782008855 35.0 35.0 37.0 30.5 39.0 84-85 33.52771501694542 35.0 35.0 36.0 30.0 38.0 86-87 33.33491707239045 35.0 35.0 36.0 30.0 37.0 88-89 33.13284788107211 35.0 34.5 36.0 30.0 37.0 90-91 32.9577764003719 35.0 34.0 35.5 30.0 36.5 92-93 32.82415122916812 35.0 34.0 35.0 30.0 36.0 94-95 32.72216001679547 35.0 34.0 35.0 29.5 36.0 96-97 32.611984764113686 35.0 34.0 35.0 29.0 36.0 98-99 32.51533835864316 35.0 34.0 35.0 29.0 36.0 100-101 32.485176502344366 35.0 34.0 35.0 29.0 36.0 102-103 31.633219030861667 34.5 32.5 35.0 26.0 35.0 104-105 32.31004878682755 35.0 34.0 35.0 29.0 35.0 106-107 32.350082977596045 35.0 34.0 35.0 29.0 35.0 108-109 32.37040374098993 35.0 34.0 35.0 29.0 35.0 110-111 32.354003169144335 35.0 34.0 35.0 29.0 35.0 112-113 32.27991817209353 35.0 34.0 35.0 29.0 35.0 114-115 32.211737830785694 35.0 34.0 35.0 29.0 35.0 116-117 32.15934947564158 35.0 34.0 35.0 29.0 35.0 118-119 32.0657434992552 35.0 34.0 35.0 29.0 35.0 120-121 31.978836964019713 35.0 34.0 35.0 29.0 35.0 122-123 31.810219990602537 35.0 34.0 35.0 27.0 35.0 124-125 29.793670708908593 33.5 29.5 34.5 13.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13824995221810354 1101 2 0.1414211944570738 1101 3 0.1756168140727894 1101 4 0.10111994273519542 1101 5 0.03992015755242306 1101 6 0.11495620332571121 1101 7 0.12077076140710119 1101 8 0.16582282900747458 1101 9 0.17153623273843976 1101 10-11 0.17691439993479463 1101 12-13 -0.018737805218023595 1101 14-15 0.12866479006738984 1101 16-17 0.22209371295841152 1101 18-19 0.11141993019517571 1101 20-21 0.10951561590178471 1101 22-23 0.06405223511450231 1101 24-25 0.0945707389219308 1101 26-27 0.09399456399762585 1101 28-29 0.0813690827003768 1101 30-31 0.19225387232012991 1101 32-33 0.16626641062975267 1101 34-35 0.11568622650315064 1101 36-37 0.01638482630043825 1101 38-39 0.0679696822128335 1101 40-41 0.11592980822600651 1101 42-43 -0.01909874049122351 1101 44-45 0.05203341833044561 1101 46-47 -0.05121613535060732 1101 48-49 0.08808104067993128 1101 50-51 -0.01063039575180369 1101 52-53 -0.13267258007236649 1101 54-55 -0.04123957061954542 1101 56-57 0.05087734658577858 1101 58-59 0.06987639424022518 1101 60-61 -0.11039795797879748 1101 62-63 -0.05735446589523008 1101 64-65 0.005231344220483436 1101 66-67 0.02568554500005149 1101 68-69 0.011815159775430573 1101 70-71 -0.09129073221746609 1101 72-73 0.030766631361842656 1101 74-75 -0.01671333595164981 1101 76-77 -0.046171303935679475 1101 78-79 -0.05388856464620062 1101 80-81 -0.06681527624433414 1101 82-83 -0.1536540270832134 1101 84-85 0.07159060370445758 1101 86-87 0.013308592976947864 1101 88-89 0.007763376166217029 1101 90-91 -0.11518386797024505 1101 92-93 0.012347770189265361 1101 94-95 -0.006325212128409419 1101 96-97 0.02120795821674193 1101 98-99 0.02420933754318355 1101 100-101 -0.09327789268469644 1101 102-103 0.08672103251453933 1101 104-105 0.01674615815073821 1101 106-107 -0.06945861448460278 1101 108-109 0.011103840735842141 1101 110-111 -0.033017551910745624 1101 112-113 -0.15863264788877984 1101 114-115 -0.04031152179090469 1101 116-117 -0.06069741937247386 1101 118-119 -0.2378578796639843 1101 120-121 -0.0632190902886336 1101 122-123 0.17442542644574033 1101 124-125 0.42313516279010344 1102 1 0.02585642750523931 1102 2 0.052253523202423935 1102 3 0.08601198145260724 1102 4 0.013376357747048928 1102 5 -0.04337895355279642 1102 6 -0.0349106611675154 1102 7 -0.0018530016249442838 1102 8 -0.03511905502553958 1102 9 -0.06846866876778535 1102 10-11 -0.06670536240839198 1102 12-13 -0.0879235590564349 1102 14-15 0.057727078938327736 1102 16-17 0.22583773510792327 1102 18-19 -0.04154514988483271 1102 20-21 -0.16606329172469714 1102 22-23 0.07578604358484142 1102 24-25 0.1245974322598471 1102 26-27 0.13813461460236454 1102 28-29 0.03985862394588935 1102 30-31 -0.08317765893690421 1102 32-33 -0.005536350110510568 1102 34-35 0.029214383669284416 1102 36-37 0.06131971575160122 1102 38-39 0.07336299496866872 1102 40-41 0.009822134056939547 1102 42-43 -0.02938693580961882 1102 44-45 -0.010404484884681153 1102 46-47 -0.031717744899275147 1102 48-49 0.0918763961716067 1102 50-51 0.04273475321424769 1102 52-53 0.1332987129979628 1102 54-55 0.13324411531313274 1102 56-57 -0.06374007098281709 1102 58-59 -0.09108343990340018 1102 60-61 -0.06497291790567061 1102 62-63 0.06513516808148268 1102 64-65 0.11524697605112522 1102 66-67 0.016377650925569753 1102 68-69 -0.05174710922452874 1102 70-71 -0.14864994638679008 1102 72-73 0.10798356937765874 1102 74-75 0.04185800745847246 1102 76-77 0.019980814972207384 1102 78-79 0.004328413111558405 1102 80-81 0.1889986387344038 1102 82-83 0.182791321122437 1102 84-85 0.10436114697681376 1102 86-87 0.1417197763595226 1102 88-89 -0.07590169199121277 1102 90-91 -0.05024387830314936 1102 92-93 0.08828634239375788 1102 94-95 0.025739911667734816 1102 96-97 -0.15593843785146078 1102 98-99 -0.33376951974351243 1102 100-101 -0.2781226341633598 1102 102-103 -0.33133936199047653 1102 104-105 -0.2039839013297069 1102 106-107 -0.365272794409627 1102 108-109 -0.4613549932355667 1102 110-111 -0.6321952248875462 1102 112-113 -0.5135828379877374 1102 114-115 -0.48360639375561476 1102 116-117 -0.5525420416253155 1102 118-119 -0.7490684192270862 1102 120-121 -0.847128902574191 1102 122-123 -0.6733828054352422 1102 124-125 -0.568502470179812 1103 1 -0.06813122627303869 1103 2 0.06475360170077948 1103 3 0.13284410172465044 1103 4 0.024187151585238098 1103 5 0.012276382137578423 1103 6 0.09632917329514612 1103 7 -0.008393484798041584 1103 8 -0.00578578867747126 1103 9 0.06872134121720563 1103 10-11 0.15469140597121367 1103 12-13 0.10026997393485715 1103 14-15 0.15052155767783404 1103 16-17 0.22404797254975506 1103 18-19 0.1860953778753256 1103 20-21 0.17968900513725572 1103 22-23 0.15601293523826598 1103 24-25 0.18184942454817588 1103 26-27 0.30283829900129433 1103 28-29 0.3119527717363155 1103 30-31 0.174731835596468 1103 32-33 0.3079044527078949 1103 34-35 0.33980412613187383 1103 36-37 0.21377451639718004 1103 38-39 0.23962565675378755 1103 40-41 0.16237741260051308 1103 42-43 0.03766950136971303 1103 44-45 -0.08037635107324093 1103 46-47 -0.04527127135950337 1103 48-49 -0.1118974908786754 1103 50-51 -0.09879307602356846 1103 52-53 -0.2485863779659354 1103 54-55 -0.13136913897758973 1103 56-57 -0.13950668594522142 1103 58-59 -0.21626320114279451 1103 60-61 -0.16266098427131936 1103 62-63 -0.11372491016568631 1103 64-65 -0.11641894749775616 1103 66-67 -0.3191007808656323 1103 68-69 -0.2662611415909666 1103 70-71 -0.2617911994717801 1103 72-73 -0.2781168834008696 1103 74-75 -0.22912146365099773 1103 76-77 -0.23475577593831076 1103 78-79 -0.41413848256097907 1103 80-81 -0.4958210348380234 1103 82-83 -0.2508618668528726 1103 84-85 -0.17310542842243137 1103 86-87 -0.2824602297947507 1103 88-89 -0.36938978222036667 1103 90-91 -0.3213787761610831 1103 92-93 -0.2697949065465224 1103 94-95 -0.22054868597479782 1103 96-97 -0.29034332610119407 1103 98-99 -0.23264793520705496 1103 100-101 -0.2377320891335657 1103 102-103 -0.23250521960073556 1103 104-105 -0.25613036042577875 1103 106-107 -0.28215088332000704 1103 108-109 -0.28690338276325633 1103 110-111 -0.23192581852352845 1103 112-113 -0.3364450137110211 1103 114-115 -0.37604637391122964 1103 116-117 -0.31599823012185624 1103 118-119 -0.39230437273094054 1103 120-121 -0.28786107245719705 1103 122-123 -0.1541616975408182 1103 124-125 -0.14766649406843513 1104 1 0.06698121553391445 1104 2 0.15980173982222823 1104 3 0.025622487080624978 1104 4 0.049451448099333106 1104 5 -0.059004492296921285 1104 6 0.03155038155900769 1104 7 -0.03152428542673391 1104 8 -0.09283481127339854 1104 9 -0.0889840451128805 1104 10-11 -0.020419601552788436 1104 12-13 0.001261123213822657 1104 14-15 0.03932279594211252 1104 16-17 0.038415901983029244 1104 18-19 0.026332691941455266 1104 20-21 0.1718856094723762 1104 22-23 0.167348252663011 1104 24-25 0.14746996108952004 1104 26-27 0.07151684631170951 1104 28-29 0.28888770907607864 1104 30-31 0.2186522687499206 1104 32-33 0.2569037415425939 1104 34-35 0.29500370062793024 1104 36-37 0.3600997639603918 1104 38-39 0.25456909222001656 1104 40-41 0.1680742051852704 1104 42-43 0.1698864844750574 1104 44-45 0.11795061762951065 1104 46-47 0.21047183661212188 1104 48-49 0.017458396554019373 1104 50-51 -0.14273741389281724 1104 52-53 -0.09349258183729603 1104 54-55 -0.05744231131266275 1104 56-57 -0.2095026819053345 1104 58-59 -0.08979829027749986 1104 60-61 -0.06007837853777431 1104 62-63 -0.15249238301889534 1104 64-65 -0.18752800678742432 1104 66-67 -0.03015103455442869 1104 68-69 -0.05623729663695798 1104 70-71 -0.10122322342430579 1104 72-73 -0.11520773924945615 1104 74-75 -0.23579386744945197 1104 76-77 -0.14148256296522277 1104 78-79 -0.09712161180780043 1104 80-81 -0.014105154337698877 1104 82-83 -0.04379623047945813 1104 84-85 -0.1530762646691528 1104 86-87 -0.08404810054194911 1104 88-89 -0.045249306157948865 1104 90-91 0.013336548554590877 1104 92-93 0.05990490998478748 1104 94-95 -0.15675446972078788 1104 96-97 -0.12176129408082659 1104 98-99 -0.040636293610461394 1104 100-101 -0.0793648409846881 1104 102-103 -0.09859249571812967 1104 104-105 -0.13972485277925983 1104 106-107 -0.09755418575649344 1104 108-109 0.023729434825831675 1104 110-111 -0.07680214150013853 1104 112-113 0.017588309462801988 1104 114-115 0.004064006113992491 1104 116-117 -0.047282331692990454 1104 118-119 -0.09219710031877781 1104 120-121 -0.21575996746170745 1104 122-123 -0.1510854594259463 1104 124-125 -0.3475397990746778 1105 1 -0.08933632056822916 1105 2 0.04875995447287096 1105 3 -0.009552801600889893 1105 4 0.11643821893549244 1105 5 -0.17034467983818047 1105 6 0.051886943061511204 1105 7 -0.05208690919582182 1105 8 0.03087550349570023 1105 9 0.07841958454287123 1105 10-11 0.08740757836564228 1105 12-13 0.11184174153933668 1105 14-15 0.118969053707346 1105 16-17 0.1200424652036034 1105 18-19 0.0072349516598748664 1105 20-21 0.05070267084376212 1105 22-23 0.1013606770451787 1105 24-25 0.08765840659494728 1105 26-27 0.07277827375929036 1105 28-29 -0.13172374414259735 1105 30-31 -0.18416808408806418 1105 32-33 -0.120285671509329 1105 34-35 -0.0516492214529265 1105 36-37 0.026843291797689517 1105 38-39 -0.09590880341156094 1105 40-41 -0.08630009967325947 1105 42-43 -0.012536722172988846 1105 44-45 -0.14260422959761598 1105 46-47 -0.3024724887728638 1105 48-49 -0.2619590861974288 1105 50-51 -0.13331513862132027 1105 52-53 -0.18139011233309787 1105 54-55 -0.1654322560630419 1105 56-57 -0.1296665318361505 1105 58-59 -0.3185597403313096 1105 60-61 -0.25424325931253833 1105 62-63 -0.22850355953735146 1105 64-65 -0.028529372203514924 1105 66-67 -0.25018195987250635 1105 68-69 -0.21830133555583586 1105 70-71 -0.18909362644810557 1105 72-73 -0.20066831851141131 1105 74-75 -0.10116926644935376 1105 76-77 -0.14841363205889024 1105 78-79 -0.07515740198585519 1105 80-81 -0.23066828448627064 1105 82-83 -0.248165561968122 1105 84-85 -0.22543427495951107 1105 86-87 -0.30759655472817826 1105 88-89 -0.23568415953396737 1105 90-91 -0.25157225792706583 1105 92-93 -0.46784197261655436 1105 94-95 -0.26624546792132975 1105 96-97 -0.3113642383157895 1105 98-99 -0.4017119882998088 1105 100-101 -0.3284357250689993 1105 102-103 -0.33578154707043595 1105 104-105 -0.18456656398019788 1105 106-107 -0.17727730469237457 1105 108-109 -0.1015316670920825 1105 110-111 -0.19105853228042236 1105 112-113 -0.10071459588944975 1105 114-115 -0.0478082415779113 1105 116-117 -0.03490316197448351 1105 118-119 0.060905830352446344 1105 120-121 -0.26994257151816825 1105 122-123 -0.08069871210026136 1105 124-125 -0.32282667209097937 1106 1 -0.017514599531850195 1106 2 0.05489053128407306 1106 3 0.02588819719088775 1106 4 -0.01445183342052303 1106 5 -0.01490547099582784 1106 6 0.02701559567707079 1106 7 -0.03360568129050989 1106 8 -0.031101495654077382 1106 9 -0.0615007627073183 1106 10-11 0.03293941709119963 1106 12-13 0.018589850905243566 1106 14-15 -0.07715123089461429 1106 16-17 -0.030425159086007625 1106 18-19 0.019397658063418532 1106 20-21 -0.006853153204211537 1106 22-23 -0.008188703184828228 1106 24-25 -0.08454367869614288 1106 26-27 -0.010915286897628107 1106 28-29 -0.029897995719991854 1106 30-31 -0.0816621548805685 1106 32-33 -0.018088508579189977 1106 34-35 0.020870579862688032 1106 36-37 0.024981744386408877 1106 38-39 0.02093462076170738 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-0.3709000411100476 2216 54-55 -0.21287105611963852 2216 56-57 -0.2928640225205612 2216 58-59 -0.25997170203122266 2216 60-61 -0.3734133456292241 2216 62-63 -0.4465781786590952 2216 64-65 -0.1973480600012607 2216 66-67 -0.40571118019229857 2216 68-69 -0.6279954537578121 2216 70-71 -0.5449600950109144 2216 72-73 -0.38412782513845656 2216 74-75 -0.2269027224184086 2216 76-77 -0.24105195100611354 2216 78-79 -0.28299193809263556 2216 80-81 -0.3228667656899802 2216 82-83 -0.2612097579966104 2216 84-85 -0.26540880446283666 2216 86-87 -0.32413468972180226 2216 88-89 -0.16772981775763185 2216 90-91 -0.27525981983451686 2216 92-93 -0.16684437816346076 2216 94-95 -0.23234612355077644 2216 96-97 -0.25972516468718254 2216 98-99 -0.30452907239961036 2216 100-101 -0.2964737893112499 2216 102-103 -0.09230836184332247 2216 104-105 -0.06477386552257514 2216 106-107 -0.18714948053312952 2216 108-109 -0.24063713381534768 2216 110-111 -0.18085736252427864 2216 112-113 -0.29574549065855393 2216 114-115 -0.20362282579564095 2216 116-117 -0.40246601278340677 2216 118-119 -0.36195040284409785 2216 120-121 -0.3622672260155966 2216 122-123 -0.6277495587584419 2216 124-125 -0.5276884710627279 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41.0 3 1.0 4 7.0 5 18.0 6 42.0 7 119.0 8 214.0 9 309.0 10 768.0 11 1110.0 12 1257.0 13 1958.0 14 3352.0 15 3565.0 16 1305.0 17 883.0 18 864.0 19 730.0 20 684.0 21 705.0 22 732.0 23 786.0 24 977.0 25 1186.0 26 1484.0 27 1913.0 28 2525.0 29 3066.0 30 3987.0 31 5105.0 32 6791.0 33 8744.0 34 12756.0 35 22140.0 36 49961.0 37 145516.0 38 113125.0 39 1382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.41668145652158 19.468365125272154 14.681951460946033 22.433001957260235 2 14.075449628600278 20.90460575644576 42.64298639367371 22.376958221280255 3 20.108320753396583 26.32989092944905 28.797974546872346 24.763813770282024 4 11.841552780749199 17.437041998660362 38.69305287572355 32.02835234486688 5 12.209746075519197 38.40898682956004 34.04645063775159 15.334816457169168 6 28.916192627990444 37.30192847931059 19.250302418347047 14.531576474351926 7 27.565557299529125 32.64493586731583 21.80986133743889 17.979645495716156 8 25.184450198446417 35.91880192348066 20.15905705459526 18.737690823477664 9 26.030471772621393 18.234326731782417 22.130024893278815 33.60517660231738 10-11 24.5370000099973 26.453607525967985 28.62014756015876 20.38924490387595 12-13 27.254766213122455 24.076124446399472 28.486933527947457 20.182175812530616 14-15 21.821733132054344 27.55056134843592 27.306502244394014 23.321203275115717 16-17 20.19457270858457 29.530063795354998 29.475453471879433 20.799910024181003 18-19 20.709408459715878 27.597048796824858 31.630334809601408 20.063207933857857 20-21 21.985025887892863 26.59985678240298 30.304018715765558 21.111098613938598 22-23 20.666049329489347 27.851904060304093 30.837991479747217 20.64405513045934 24-25 21.561678346846353 26.873994021614163 31.780919151829007 19.78340847971048 26-27 20.199921021324243 27.545687664330632 32.03172643386286 20.22266488048227 28-29 19.70542953402581 28.55641476801264 30.479770461975264 21.25838523598628 30-31 20.563197936557128 26.816134643646215 31.213822267987645 21.406845151809012 32-33 20.166929928919192 27.48245473722095 31.94449998500405 20.406115348855806 34-35 20.927974446899338 27.60692113129455 31.52423845561698 19.94086596618913 36-37 20.410489167924663 27.087436392174112 31.610840073180242 20.891234366720983 38-39 20.81088106211323 28.078793725694062 30.78718745938597 20.32313775280674 40-41 21.855848920791388 26.784518180091375 31.245313765283374 20.114319133833867 42-43 20.22228998170494 28.13915242884421 30.829551021224272 20.80900656822658 44-45 20.431483499455148 27.94557969348276 30.193472762354162 21.42946404470793 46-47 22.040923950533355 27.542688474111987 29.60000799784058 20.81637957751407 48-49 21.505818429024163 27.902966199126233 29.407060093774678 21.184155278074922 50-51 20.235536405170603 28.85658372239495 29.915672768352547 20.9922071040819 52-53 20.74964759514931 28.487183460465676 29.673488158197287 21.089680786187728 54-55 20.564947464184673 27.6785267977646 29.853439571315743 21.90308616673498 56-57 21.395723154748218 27.855603986923533 28.646890339608305 22.101782518719943 58-59 21.98606376278405 28.108160796584926 28.3219780659222 21.58379737470883 60-61 21.024323432673178 27.212152718765932 30.894658442220603 20.868865406340287 62-63 20.93797174762814 27.30025393143851 30.36855049136733 21.393223829566015 64-65 21.044068101612563 28.56228818219081 28.900571845601686 21.49307187059494 66-67 21.16141141891689 28.91956671698641 28.25024743319304 21.668774430903657 68-69 21.55830425785038 27.42759454947164 29.795455227088684 21.218645965589293 70-71 22.596648904795703 27.2341467803693 29.21098803323103 20.958216281603967 72-73 21.77349615603787 27.757505473522148 29.504533775880514 20.964464594559466 74-75 20.767142871424717 29.12126225919002 29.59550921251262 20.516085656872647 76-77 21.316369580213344 27.328371339738272 30.004523778579784 21.350735301468603 78-79 21.273506153338598 27.52319373769082 29.65349355673968 21.549806552230898 80-81 21.712137722814838 27.89684285242984 29.330330810681115 21.0606886140742 82-83 21.33036580123367 27.765003449068754 30.283198536395172 20.62143221330241 84-85 20.896108050826275 28.210383196536938 30.020644426004978 20.87286432663181 86-87 20.28014935967289 29.216611514890978 28.590530556749677 21.912708568686455 88-89 20.65629779959411 28.280364301638556 28.931938376638307 22.131399522129026 90-91 21.26034730969948 27.99484633995161 29.44596904804751 21.2988373023014 92-93 21.0569396263009 28.802973197236746 29.536150239435354 20.603936937027004 94-95 20.758348089821084 28.8013316454494 29.324317400492117 21.116002864237394 96-97 21.147165265378348 28.343222329970907 29.46466953922441 21.044942865426336 98-99 21.431240400849287 28.011212182939037 29.523082325565703 21.034465090645977 100-101 20.663972387386373 27.773320636729675 29.880132064672697 21.68257491121125 102-103 20.87152122567826 28.091875882580826 29.388535509428777 21.648067382312146 104-105 21.636992798041486 27.726103131845953 29.02540493052353 21.61149913958903 106-107 21.038864629912442 27.65814922478198 29.59438213761713 21.708604007688443 108-109 20.537109008885167 28.17622873995576 29.113107809200084 22.173554441958988 110-111 20.092705184266862 28.969932676890313 29.20413261665319 21.73322952218963 112-113 20.951867955253558 27.521611491802194 28.823737460210737 22.702783092733515 114-115 20.453182097669977 28.169225308811697 28.033878054998723 23.343714538519603 116-117 21.255258047343045 28.03629583530159 27.562165553197815 23.14628056415755 118-119 20.909682796955313 27.18563682419556 29.107625094818506 22.797055284030627 120-121 20.287924419839793 27.94179028005149 29.17496657127504 22.59531872883368 122-123 20.994843827714362 27.890943082156344 28.418807157157033 22.69540593297226 124-125 21.549254939990302 27.934126797666597 28.019355064458562 22.49726319788454 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 78.0 1 60.0 2 168.0 3 229.5 4 98.0 5 38.0 6 96.5 7 270.0 8 472.0 9 500.5 10 363.0 11 240.0 12 215.5 13 258.5 14 299.0 15 347.0 16 424.0 17 497.5 18 553.0 19 624.0 20 727.0 21 869.0 22 1050.0 23 1264.5 24 1562.0 25 1967.5 26 2420.0 27 2980.5 28 3546.0 29 4224.5 30 5289.5 31 6669.0 32 8540.5 33 10663.0 34 13311.5 35 16107.0 36 18157.5 37 18917.0 38 17834.5 39 15836.5 40 14200.0 41 13271.5 42 12496.0 43 12052.0 44 11915.0 45 12021.5 46 12089.5 47 13483.0 48 17393.5 49 16757.5 50 12755.0 51 11239.5 52 10334.5 53 9725.5 54 9339.0 55 8625.0 56 7642.5 57 6829.0 58 6038.5 59 5154.0 60 4223.5 61 3449.5 62 2854.0 63 2135.5 64 1526.5 65 1169.5 66 909.0 67 738.5 68 531.5 69 339.5 70 259.5 71 194.5 72 153.5 73 162.5 74 132.0 75 88.5 76 61.5 77 32.5 78 15.0 79 8.0 80 6.5 81 5.5 82 5.0 83 2.5 84 1.5 85 0.5 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 2.5 99 3.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014746018574984756 2 0.0 3 0.0 4 0.0 5 0.12521619162826036 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.249662591100403E-4 18-19 0.0 20-21 0.00437381906885141 22-23 6.248312955502015E-4 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 9.997300728803223E-4 92-93 0.0 94-95 3.7489877733012087E-4 96-97 0.0 98-99 0.0018744938866506044 100-101 2.499325182200806E-4 102-103 7.497975546602417E-4 104-105 0.0021244264048706847 106-107 0.006748177991942175 108-109 7.497975546602417E-4 110-111 0.005623481659951813 112-113 0.007872874323932539 114-115 0.006498245473722095 116-117 0.001749527627540564 118-119 3.7489877733012087E-4 120-121 7.497975546602417E-4 122-123 0.001249662591100403 124-125 0.0014995951093204835 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 400108.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.29707100375627 #Duplication Level Percentage of deduplicated Percentage of total 1 90.30419494691961 68.89945573802173 2 6.520819219651762 9.95038814008858 3 1.3415417214625545 3.070671119307898 4 0.5411517501253437 1.6515317401248124 5 0.32793238908144257 1.2510140387089126 6 0.18708117777472325 0.8564247542486645 7 0.1279897603126016 0.6835670681227017 8 0.10719996635895389 0.6543234755911511 9 0.07380937556889161 0.5068295251670373 >10 0.41912565892958675 5.558974858574836 >50 0.030147807417861966 1.6766380580192692 >100 0.014746210135365898 2.080921346034392 >500 0.0029492420270731796 1.4123662962571524 >1k 0.0013107742342547465 1.7468938417328241 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3037 0.7590450578343847 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1455 0.36365181401021723 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1400 0.3499055255081128 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1095 0.27367610745098825 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 857 0.21419216811460906 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 762 0.1904485788837014 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 745 0.18619972607396001 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 727 0.18170094074599857 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 529 0.1322143021384226 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 511 0.12771551681046117 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 511 0.12771551681046117 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 506 0.1264658542193608 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 501 0.12521619162826036 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 421 0.10522159017065393 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 401 0.10022293980625231 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.499325182200806E-4 0.0 12-13 0.0 0.0 0.0 2.499325182200806E-4 0.0 14-15 0.0 0.0 0.0 2.499325182200806E-4 0.0 16-17 0.0 0.0 0.0 2.499325182200806E-4 0.0 18-19 0.0 0.0 0.0 2.499325182200806E-4 0.0 20-21 0.0 0.0 0.0 2.499325182200806E-4 0.0 22-23 0.0 0.0 0.0 2.499325182200806E-4 0.0 24-25 0.0 0.0 0.0 3.7489877733012087E-4 0.0 26-27 0.0 0.0 0.0 7.497975546602417E-4 0.0 28-29 0.0 0.0 0.0 0.001749527627540564 0.0 30-31 0.0 0.0 0.0 0.004123886550631329 0.0 32-33 0.0 0.0 0.0 0.007997840583042579 0.0 34-35 0.0 0.0 0.0 0.01274655842922411 0.0 36-37 0.0 0.0 0.0 0.019869635198496407 0.0 38-39 0.0 0.0 0.0 0.03299109240505063 0.0 40-41 0.0 0.0 0.0 0.04861187479380567 0.0 42-43 0.0 0.0 0.0 0.06685694862387156 0.0 44-45 0.0 0.0 0.0 0.08997570655922901 0.0 46-47 1.249662591100403E-4 0.0 0.0 0.11559378967678727 0.0 48-49 2.499325182200806E-4 0.0 0.0 0.14346126545832627 0.0 50-51 2.499325182200806E-4 0.0 0.0 0.17470283023583633 0.0 52-53 2.499325182200806E-4 0.0 0.0 0.20731902386355683 0.0 54-55 2.499325182200806E-4 0.0 0.0 0.2459335979285593 0.0 56-57 2.499325182200806E-4 1.249662591100403E-4 0.0 0.29392064142681473 0.0 58-59 2.499325182200806E-4 2.499325182200806E-4 0.0 0.34690633528947185 0.0 60-61 2.499325182200806E-4 2.499325182200806E-4 0.0 0.40101672548411926 0.0 62-63 2.499325182200806E-4 2.499325182200806E-4 0.0 0.4698731342537515 0.0 64-65 2.499325182200806E-4 2.499325182200806E-4 0.0 0.5352304877683025 0.0 66-67 2.499325182200806E-4 2.499325182200806E-4 0.0 0.6092105131614464 0.0 68-69 2.499325182200806E-4 2.499325182200806E-4 0.0 0.709933318004139 0.0 70-71 2.499325182200806E-4 3.7489877733012087E-4 0.0 0.8206534235756346 0.0 72-73 2.499325182200806E-4 4.998650364401612E-4 0.0 0.9296240015195898 0.0 74-75 2.499325182200806E-4 4.998650364401612E-4 0.0 1.0304717726213921 0.0 76-77 2.499325182200806E-4 4.998650364401612E-4 0.0 1.1434412708568686 0.0 78-79 2.499325182200806E-4 4.998650364401612E-4 0.0 1.268657462485129 0.0 80-81 4.998650364401612E-4 4.998650364401612E-4 0.0 1.4118687954252351 0.0 82-83 4.998650364401612E-4 6.248312955502015E-4 0.0 1.5589540823977526 0.0 84-85 4.998650364401612E-4 7.497975546602417E-4 0.0 1.70591440311116 0.0 86-87 4.998650364401612E-4 7.497975546602417E-4 0.0 1.8556239815249884 0.0 88-89 4.998650364401612E-4 7.497975546602417E-4 0.0 2.019579713477361 0.0 90-91 4.998650364401612E-4 7.497975546602417E-4 0.0 2.2011556879642495 0.0 92-93 4.998650364401612E-4 7.497975546602417E-4 0.0 2.409724374418907 0.0 94-95 4.998650364401612E-4 7.497975546602417E-4 0.0 2.6201675547602146 0.0 96-97 4.998650364401612E-4 7.497975546602417E-4 0.0 2.8333599928019435 0.0 98-99 4.998650364401612E-4 7.497975546602417E-4 0.0 3.0599238205684465 0.0 100-101 4.998650364401612E-4 7.497975546602417E-4 0.0 3.3139802253391584 0.0 102-103 4.998650364401612E-4 7.497975546602417E-4 0.0 3.574909774360923 0.0 104-105 7.497975546602417E-4 7.497975546602417E-4 0.0 3.8474611854799203 0.0 106-107 7.497975546602417E-4 7.497975546602417E-4 0.0 4.106516240615034 0.0 108-109 7.497975546602417E-4 7.497975546602417E-4 0.0 4.391564277645036 0.0 110-111 7.497975546602417E-4 7.497975546602417E-4 0.0 4.7134773611125 0.0 112-113 7.497975546602417E-4 7.497975546602417E-4 0.0 5.080628230377798 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 490 0.0 47.40524 2 GTACCTG 535 0.0 43.43418 1 GTACATG 2320 0.0 32.102795 1 TACATGG 2350 0.0 31.68108 2 ACATGGG 2340 0.0 30.289278 3 ACCTGGG 735 0.0 29.172457 3 GAGTACT 1670 0.0 29.034224 12-13 GATCTAC 110 7.6796935E-4 27.083088 1 GATCGCG 45 0.009526582 26.444832 94-95 TAGGACC 475 0.0 26.33198 4 AGTACTT 1770 0.0 26.217445 12-13 CATGGGG 1255 0.0 26.10218 4 GTACTTT 1925 0.0 24.879076 14-15 AGAGTAC 2800 0.0 24.859758 10-11 TCGTTAC 60 0.0012883481 24.788933 52-53 AGGGCGC 65 0.0020529341 22.882092 62-63 GTGTAAG 210 2.7491678E-6 22.698204 1 GTACAGC 135 0.0025540337 22.034607 6 CATGACT 190 3.2729407E-5 21.943316 4 TATACTG 275 5.433867E-8 21.658337 5 >>END_MODULE