FastQCFastQC Report
Fri 27 May 2016
SRR939976_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939976_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1746718
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA41130.23547017892985586No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC34190.19573852218847002No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA26680.15274360257351216No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA21540.12331698648551169No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA21080.1206834760963132No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17510.10024514546709887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGCT350.04283.2524110-111
CCGCTCC4000.02809.9458108-109
CCGCTCG1100.02724.796108-109
CCGTTCG600.02497.7295108-109
CCGCGCC2900.02325.4724108-109
CGTCGCC1200.01665.153108-109
CCGCGCG1150.01520.3572108-109
CGACGTT200.003636161499.1383110-111
CCGCGCT501.083572E-81498.6377108-109
CGTCGCG702.3646862E-111427.2742108-109
CGACGGC200.0052408581248.8649108-109
CGTCGCA250.0056808711199.3107110-111
CGACGCC451.5867481E-51110.102108-109
CATCGCG451.5867481E-51110.102108-109
CGTCGAC250.008187752999.0918108-109
CCGCGCA250.008187752999.0918108-109
CGCCGCC1900.0946.82416110-111
CGGCGCG601.01614205E-7832.5765108-109
CCGCGAC306.468159E-5832.5765108-109
CGGCGCC1551.8189894E-12805.7192108-109
Produced by FastQC (version 0.11.5)